GPCRdb

Ligand source activities (1 row/activity)

Potency
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Ligands					Receptor			Assay information							Chemical information
Sel. page	Common name	GPCRdb ID	#Vendors	Reference ligand	Fold selectivity (Potency)	# tested GPCRs (Potency)	Species	p-value (-log)	Type	Activity Relation	Activity Value	Assay Type	Assay Description	Source	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles	DOI
	155524115	175963	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175963	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175963	0	None	-1	3	Rat	10.3	pEC50	=	10.3	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	155524115	175963	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175963	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175963	0	None	1	3	Human	9.9	pEC50	=	9.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	155524115	175963	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175963	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175963	0	None	-4	3	Mouse	9.6	pEC50	=	9.6	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	46235428	6511	0	None	263	3	Human	9.3	pEC50	=	9.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6511	0	None	263	3	Human	9.3	pEC50	=	9.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	58227401	175867	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175867	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175867	0	None	2	3	Human	9.2	pEC50	=	9.2	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	58227401	175867	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175867	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175867	0	None	-2	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-18	3	Rat	9.2	pEC50	=	9.2	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	51354300	58566	0	None	-	1	Human	9.0	pEC50	=	9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688946	58566	0	None	-	1	Human	9.0	pEC50	=	9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	155491015	175911	3	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175911	3	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175911	3	None	-10	4	Mouse	9.0	pEC50	=	9	Functional	Agonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assayAgonist activity at mouse H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	155513612	175736	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-14	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	58227401	175867	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175867	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175867	0	None	-2	3	Mouse	9.0	pEC50	=	9.0	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	45257132	3613	3	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3613	3	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3613	3	None	-	1	Human	9.0	pEC50	=	9.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	155552061	175894	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175894	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175894	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	155517880	176083	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	2	3	Rat	8.9	pEC50	=	8.9	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	45257132	3613	3	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3613	3	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3613	3	None	-	1	Human	8.9	pEC50	=	8.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	58227373	175924	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175924	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175924	0	None	-1	3	Rat	8.8	pEC50	=	8.8	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	14	3	Human	8.7	pEC50	=	8.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	136030655	56173	0	None	1	3	Mouse	8.0	pEC50	=	8	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56173	0	None	1	3	Mouse	8.0	pEC50	=	8	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136030655	56173	0	None	-1	3	Rat	8.0	pEC50	=	8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56173	0	None	-1	3	Rat	8.0	pEC50	=	8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25068753	187844	15	None	-1	2	Rat	8.0	pEC50	=	8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187844	15	None	-1	2	Rat	8.0	pEC50	=	8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	10105117	103705	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL309819	103705	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	127051301	139657	0	None	4	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139657	0	None	4	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	136141537	56172	1	None	1	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56172	1	None	1	3	Human	7.0	pEC50	=	7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	4024	1976	14	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	9989505	1976	14	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL82298	1976	14	None	-3388	2	Human	6.0	pEC50	=	6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	58227259	116627	0	None	-	1	Human	6.0	pEC50	=	6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	116627	0	None	-	1	Human	6.0	pEC50	=	6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1194	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	23786499	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	CHEMBL26116	46	3	None	-50	2	Human	5.0	pEC50	=	5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	24797598	61218	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771001	61218	0	None	-	1	Human	7.0	pEC50	=	7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	11846299	138677	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	CHEMBL378788	138677	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	46228114	198482	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596551	198482	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25130576	187033	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187033	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130232	186970	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cn(C)cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494576	186970	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cn(C)cn3)nc(N)n2)CC1	10.1021/jm8005959
	25131229	187002	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	CHEMBL494724	187002	0	None	-	1	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	25130576	187033	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187033	1	None	2	2	Mouse	7.0	pEC50	=	7.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	11846635	78255	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	CHEMBL211242	78255	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	46228132	200450	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL609161	200450	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as inhibition of 100 nM dopamine-induced stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	25129178	187036	1	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL494910	187036	1	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	1267	3732	43	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	3035905	3732	43	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	CHEMBL260374	3732	43	None	-17	5	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm900526h
	44480708	5683	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(/NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1021/jm900526h
	CHEMBL1078632	5683	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(/NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1021/jm900526h
	53233804	61182	0	None	-	1	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770966	61182	0	None	-	1	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	1204	1893	114	None	-1	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1893	114	None	-1	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1893	114	None	-1	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1893	114	None	-1	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1893	114	None	-1	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1893	114	None	-1	11	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	127052293	139695	1	None	10	2	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139695	1	None	10	2	Human	8.0	pEC50	=	8.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1247	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1375	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	774	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	CHEMBL90	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	DB05381	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	45257131	198216	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198216	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	56593499	139699	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	6	3	4	3.2	N#C/N=C(/NCCSc1ccccc1)N[C@H]1CC[C@@H](c2c[nH]cn2)CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805392	139699	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	6	3	4	3.2	N#C/N=C(/NCCSc1ccccc1)N[C@H]1CC[C@@H](c2c[nH]cn2)CC1	10.1021/acs.jmedchem.6b00120
	127050776	139704	0	None	3	3	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	139704	0	None	3	3	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	127050431	139772	0	None	3	2	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139772	0	None	3	2	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227165	116607	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393536	116607	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	25130578	186740	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	251	4	1	5	1.4	CN(C)CCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL493099	186740	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	251	4	1	5	1.4	CN(C)CCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	25129525	186990	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494648	186990	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	25128833	187808	30	None	-2	2	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	CHEMBL501882	187808	30	None	-2	2	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	25128831	172034	0	None	1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL449157	172034	0	None	1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	11043445	56170	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL1632410	56170	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	16061839	187824	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	284	3	2	6	1.6	CN1CCN(c2cc(Nc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL502104	187824	0	None	-	1	Rat	6.9	pEC50	=	6.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	284	3	2	6	1.6	CN1CCN(c2cc(Nc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	127050431	139772	0	None	3	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139772	0	None	3	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	54585780	61214	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770998	61214	0	None	-	1	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	1204	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	1247	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	1375	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	774	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	CHEMBL90	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	DB05381	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm900526h
	25130914	186661	0	None	15	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL492677	186661	0	None	15	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	11957573	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	1250	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	3692	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	CHEMBL19439	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm100064d
	11957573	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1250	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3692	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19439	1971	29	None	-54	5	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1253	2019	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	132937	2019	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19010	2019	7	None	-251	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10176414	3988	13	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1246	3988	13	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL281576	3988	13	None	-25	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1267	3732	43	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-17	5	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	44482032	5640	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	262	7	3	3	1.4	CC(C)CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1078346	5640	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	262	7	3	3	1.4	CC(C)CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	25130581	186632	0	None	3	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL492489	186632	0	None	3	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	44482029	5545	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1cccnc1	10.1021/jm900526h
	CHEMBL1077737	5545	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1cccnc1	10.1021/jm900526h
	25131234	187069	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccccc3C#N)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495083	187069	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccccc3C#N)nc(N)n2)CC1	10.1021/jm8005959
	25130235	186994	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	186994	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Agonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at mouse histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	186994	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	186994	1	None	4	3	Mouse	6.9	pEC50	=	6.9	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	25130234	186810	0	None	-	1	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	2	6	-0.7	CN1CCN(c2cc(N3CCNC3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493663	186810	0	None	-	1	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	2	6	-0.7	CN1CCN(c2cc(N3CCNC3=O)nc(N)n2)CC1	10.1021/jm8005959
	127051986	139761	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806098	139761	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	51354301	58569	0	None	-1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	CHEMBL1688969	58569	0	None	-1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	10934158	108077	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL320331	108077	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	10017821	103297	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL308913	103297	0	None	-	1	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1238	2043	11	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	6603865	2043	11	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL11919	2043	11	None	-1258	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	11043445	56170	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632410	56170	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	10945113	109128	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL322256	109128	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	44480707	5671	0	None	5	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	328	8	3	4	2.2	N#C/N=C(/NCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078529	5671	0	None	5	2	Human	5.9	pEC50	=	5.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	328	8	3	4	2.2	N#C/N=C(/NCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	10945113	109128	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL322256	109128	1	None	-1	2	Human	4.9	pEC50	=	4.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	127052294	139759	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139759	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1247	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	1375	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	774	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	CHEMBL90	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	DB05381	1893	114	None	-1	11	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2009.08.059
	10934158	108077	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL320331	108077	1	None	1	2	Human	6.9	pEC50	=	6.9	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	25129182	186690	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	291	2	1	7	-0.4	CN1CCN(c2cc(N3CCN(C)CC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492869	186690	0	None	-	1	Rat	5.9	pEC50	=	5.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	291	2	1	7	-0.4	CN1CCN(c2cc(N3CCN(C)CC3)nc(N)n2)CC1	10.1021/jm8005959
	127052293	139695	1	None	10	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139695	1	None	10	2	Human	7.9	pEC50	=	7.9	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11846634	77640	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209758	77640	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	11846464	78036	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	CHEMBL211170	78036	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	25071319	188464	8	None	1	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188464	8	None	1	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	44482030	5584	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	338	9	3	3	2.9	CC(CCN/C(=N\C#N)NCCCCc1c[nH]cn1)c1ccccc1	10.1021/jm900526h
	CHEMBL1077860	5584	0	None	-	1	Human	6.9	pEC50	=	6.9	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	338	9	3	3	2.9	CC(CCN/C(=N\C#N)NCCCCc1c[nH]cn1)c1ccccc1	10.1021/jm900526h
	25131561	186811	0	None	-3	2	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493664	186811	0	None	-3	2	Rat	4.9	pEC50	=	4.9	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	135897837	187035	12	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	209	1	2	6	-0.5	CN1CCN(c2cc(O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494909	187035	12	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	209	1	2	6	-0.5	CN1CCN(c2cc(O)nc(N)n2)CC1	10.1021/jm8005959
	127048828	139777	0	None	38	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806255	139777	0	None	38	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227255	116610	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393539	116610	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	44568832	186590	0	None	-	1	Rat	7.8	pEC50	=	7.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	2	1	6	1.2	CN1CCN(c2cc(-c3ccc(F)nc3F)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492242	186590	0	None	-	1	Rat	7.8	pEC50	=	7.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	2	1	6	1.2	CN1CCN(c2cc(-c3ccc(F)nc3F)nc(N)n2)CC1	10.1021/jm8005959
	25128822	186888	0	None	19	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	186888	0	None	19	2	Rat	7.8	pEC50	=	7.8	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	1251	1975	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	3035842	1975	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	CHEMBL18661	1975	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptorAgonist activity at human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm100064d
	1251	1975	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	3035842	1975	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL18661	1975	34	None	-204	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	25149712	178398	0	None	-6	4	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	299	7	4	3	1.6	N=C(NCCCc1c[nH]cn1)NC(=O)CCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL470790	178398	0	None	-6	4	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	299	7	4	3	1.6	N=C(NCCCc1c[nH]cn1)NC(=O)CCc1ccccc1	10.1016/j.bmcl.2010.10.041
	25149335	183633	0	None	-6	4	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL483406	183633	0	None	-6	4	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	1278	2110	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2110	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2110	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2110	79	None	1	4	Human	7.8	pEC50	=	7.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	127048890	139752	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805995	139752	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	46890548	6491	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083084	6491	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44481761	5682	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	324	9	3	3	2.4	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccc1	10.1021/jm900526h
	CHEMBL1078631	5682	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	324	9	3	3	2.4	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccc1	10.1021/jm900526h
	51354301	58569	0	None	-1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688969	58569	0	None	-1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	155513612	175736	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-18	3	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at rat H4R expressed in in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	1204	1893	114	None	-97	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-97	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-97	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-97	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-97	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-97	11	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1248	1391	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	3077	1391	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	90045	1391	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	CHEMBL12344	1391	27	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	5310982	93166	4	None	-3	2	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	CHEMBL24665	93166	4	None	-3	2	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	1269	121	33	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	-35	6	Mouse	5.8	pEC50	=	5.8	Functional	Agonist activity at mouse histamine H4 receptorAgonist activity at mouse histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	53233802	61203	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770987	61203	0	None	-	1	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	25131559	186992	0	None	23	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494667	186992	0	None	23	2	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	1204	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-1	11	Human	7.8	pEC50	=	7.8	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	44480959	5585	2	None	1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	206	5	3	3	0.1	N#C/N=C(\N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077861	5585	2	None	1	2	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	206	5	3	3	0.1	N#C/N=C(\N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	1484519	173314	52	None	-	1	Rat	4.8	pEC50	=	4.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	227	1	1	5	0.5	CN1CCN(c2cc(Cl)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL454007	173314	52	None	-	1	Rat	4.8	pEC50	=	4.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	227	1	1	5	0.5	CN1CCN(c2cc(Cl)nc(N)n2)CC1	10.1021/jm8005959
	21638785	188063	4	None	1	2	Mouse	7.8	pEC50	=	7.8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188063	4	None	1	2	Mouse	7.8	pEC50	=	7.8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	127051958	139735	0	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805813	139735	0	None	-	1	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	165	2	2	2	1.3	NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25129880	192518	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	2	1	5	2.6	CN1CCN(c2cc(-c3ccc4ccccc4c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL522841	192518	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	2	1	5	2.6	CN1CCN(c2cc(-c3ccc4ccccc4c3)nc(N)n2)CC1	10.1021/jm8005959
	25130231	187111	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	309	2	1	7	1.2	CN1CCN(c2cc(-n3cnc4ccccc43)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495280	187111	0	None	-	1	Human	6.8	pEC50	=	6.8	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	309	2	1	7	1.2	CN1CCN(c2cc(-n3cnc4ccccc43)nc(N)n2)CC1	10.1021/jm8005959
	25131228	187001	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL494723	187001	0	None	-	1	Rat	6.8	pEC50	=	6.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25128828	187659	2	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	237	4	2	5	1.3	CN(C)CCNc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL499631	187659	2	None	-	1	Rat	5.8	pEC50	=	5.8	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	237	4	2	5	1.3	CN(C)CCNc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	58227163	175879	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175879	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175879	0	None	4	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25071319	188464	8	None	-1	2	Mouse	7.7	pEC50	=	7.7	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188464	8	None	-1	2	Mouse	7.7	pEC50	=	7.7	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1204	1893	114	None	-1	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	1247	1893	114	None	-1	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	1375	1893	114	None	-1	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	774	1893	114	None	-1	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	CHEMBL90	1893	114	None	-1	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	DB05381	1893	114	None	-1	11	Human	5.8	pEC50	=	5.8	Functional	Agonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assayAgonist activity at human H4 receptor expressed in 293-EBNA cells by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0603318
	25130233	170036	6	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3cn[nH]c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL445091	170036	6	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3cn[nH]c3)nc(N)n2)CC1	10.1021/jm8005959
	25128831	172034	0	None	-1	2	Rat	4.7	pEC50	=	4.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL449157	172034	0	None	-1	2	Rat	4.7	pEC50	=	4.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	277	2	0	5	1.6	CN1CCN(c2cc(C(C)(C)C)nc(N(C)C)n2)CC1	10.1021/jm8005959
	25130574	186783	0	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(N)nc(-c3ccc(C#N)cc3)c2)CC1	10.1021/jm8005959
	CHEMBL493461	186783	0	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(N)nc(-c3ccc(C#N)cc3)c2)CC1	10.1021/jm8005959
	44568790	170838	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	2	2	5	1.4	CN[C@@H]1CCN(c2nc(N)nc3c2Cc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL446269	170838	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	2	2	5	1.4	CN[C@@H]1CCN(c2nc(N)nc3c2Cc2ccccc2-3)C1	10.1021/jm800670r
	155552061	175894	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175894	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175894	0	None	-2	3	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	45257133	198212	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198212	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	11846300	79841	3	None	-2	2	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79841	3	None	-2	2	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	1204	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3032915	15306	20	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL12160	15306	20	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	53321919	56169	0	None	-50	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632409	56169	0	None	-50	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10060213	102425	1	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL306077	102425	1	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	25128823	192105	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192105	0	None	-	1	Rat	7.7	pEC50	=	7.7	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	25131230	192497	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	329	4	1	7	1.5	COc1cccc(OC)c1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL522679	192497	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	329	4	1	7	1.5	COc1cccc(OC)c1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	10037650	5123	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106158	5123	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	170389	205243	31	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL4634086	205243	31	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL91353	205243	31	None	-4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	155513612	175736	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-14	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293T cells by CRE-luciferase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	136141537	56172	1	None	-1	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56172	1	None	-1	3	Mouse	6.7	pEC50	=	6.7	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	136192874	56174	0	None	-3	3	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56174	0	None	-3	3	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	46228133	198361	0	None	-	1	Human	6.7	pEC50	=	6.7	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595639	198361	0	None	-	1	Human	6.7	pEC50	=	6.7	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10197870	5214	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106612	5214	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10197870	5214	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106612	5214	0	None	-47	2	Human	5.7	pEC50	=	5.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10879463	5203	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106570	5203	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	1278	2110	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2110	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2110	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2110	79	None	-1	4	Mouse	8.6	pEC50	=	8.6	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1278	2110	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2110	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2110	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2110	79	None	-1	4	Rat	8.6	pEC50	=	8.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	58227260	116617	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393546	116617	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	11846636	77583	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209628	77583	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	1204	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1247	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1375	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	774	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	CHEMBL90	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	DB05381	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	16061961	197987	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197987	0	None	-	1	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	1204	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	1247	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	1375	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	774	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	CHEMBL90	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	DB05381	1893	114	None	-1	11	Human	7.7	pEC50	=	7.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm0300025
	127052635	139664	0	None	4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805003	139664	0	None	4	2	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	288	5	3	3	2.0	CC(C)CN/C(=N/C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130237	186689	2	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	1	1	5	0.4	CN1CCN(c2cc(I)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492864	186689	2	None	-	1	Rat	5.7	pEC50	=	5.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	319	1	1	5	0.4	CN1CCN(c2cc(I)nc(N)n2)CC1	10.1021/jm8005959
	127051301	139657	0	None	4	3	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139657	0	None	4	3	Human	6.7	pEC50	=	6.7	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25131555	186586	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3cccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492227	186586	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3cccnc3)nc(N)n2)CC1	10.1021/jm8005959
	25130914	186661	0	None	15	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL492677	186661	0	None	15	2	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10879463	5203	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106570	5203	0	None	-	1	Human	4.7	pEC50	=	4.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	1613	2306	44	None	-31	9	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	205	2306	44	None	-31	9	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	3964	2306	44	None	-31	9	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	CHEMBL831	2306	44	None	-31	9	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	DB00408	2306	44	None	-31	9	Human	6.7	pEC50	=	6.7	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	753256	178760	10	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL473662	178760	10	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1021/jm8005959
	25131560	186753	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cnn(C)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493229	186753	0	None	-	1	Rat	6.7	pEC50	=	6.7	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	273	2	1	7	0.2	CN1CCN(c2cc(-c3cnn(C)c3)nc(N)n2)CC1	10.1021/jm8005959
	1223	938	32	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	2790	938	32	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	CHEMBL14690	938	32	None	-21	4	Human	7.7	pEC50	=	7.7	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	155512725	175657	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175657	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175657	0	None	-1	3	Rat	7.6	pEC50	=	7.6	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	10037650	5123	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106158	5123	0	None	47	2	Human	6.7	pEC50	=	6.7	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	25129881	186589	4	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3ccncc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492241	186589	4	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	270	2	1	6	0.9	CN1CCN(c2cc(-c3ccncc3)nc(N)n2)CC1	10.1021/jm8005959
	44568855	192581	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	285	2	1	5	2.0	CC(C)(C)c1cc(N2CCNCC2)nc(-n2cccc2)n1	10.1021/jm8005959
	CHEMBL523335	192581	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	285	2	1	5	2.0	CC(C)(C)c1cc(N2CCNCC2)nc(-n2cccc2)n1	10.1021/jm8005959
	25128832	171436	0	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	5	1.2	CN1CCN(c2cc(C(C)(C)C)nc(O)n2)CC1	10.1021/jm8005959
	CHEMBL447151	171436	0	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	5	1.2	CN1CCN(c2cc(C(C)(C)C)nc(O)n2)CC1	10.1021/jm8005959
	25071940	186761	0	None	-	1	Rat	7.6	pEC50	=	7.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	186761	0	None	-	1	Rat	7.6	pEC50	=	7.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	127049156	139651	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804837	139651	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	11437850	187068	1	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3cccc(C#N)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL495082	187068	1	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3cccc(C#N)c3)nc(N)n2)CC1	10.1021/jm8005959
	25129883	192111	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ccncc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL522019	192111	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ccncc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25133972	186757	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(-c3ccc(C#N)cc3)cc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493262	186757	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	293	2	1	5	2.0	CN1CCN(c2cc(-c3ccc(C#N)cc3)cc(N)n2)CC1	10.1021/jm8005959
	25130575	186996	1	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.6	CC1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494687	186996	1	None	-	1	Rat	4.6	pEC50	=	4.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.6	CC1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	25130577	172019	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	4	1	6	1.0	CN(C)CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL449021	172019	0	None	-	1	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	306	4	1	6	1.0	CN(C)CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	10773349	7992	1	None	-89	2	Human	4.6	pEC50	=	4.6	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL109130	7992	1	None	-89	2	Human	4.6	pEC50	=	4.6	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	127052634	139766	0	None	6	2	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806146	139766	0	None	6	2	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	272	4	3	3	1.5	N#C/N=C(\NC[C@@H]1CC[C@H](c2c[nH]cn2)C1)NC1CC1	10.1021/acs.jmedchem.6b00120
	90667240	108978	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	356	9	3	4	2.8	Cc1[nH]cnc1CCCCN/C(=N\C#N)NCCSc1ccccc1	10.1039/C3MD00245D
	CHEMBL3220642	108978	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	356	9	3	4	2.8	Cc1[nH]cnc1CCCCN/C(=N\C#N)NCCSc1ccccc1	10.1039/C3MD00245D
	25130576	187033	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187033	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25128826	188219	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.5	CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL506187	188219	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.5	CCN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	25130576	187033	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187033	1	None	-2	2	Rat	6.6	pEC50	=	6.6	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	58227157	115404	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354815	115404	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	1204	1893	114	None	-1	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1247	1893	114	None	-1	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1375	1893	114	None	-1	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	774	1893	114	None	-1	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	CHEMBL90	1893	114	None	-1	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	DB05381	1893	114	None	-1	11	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1226	1980	16	None	-1	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	41376	1980	16	None	-1	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	CHEMBL12608	1980	16	None	-1	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	25133970	186995	2	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL494679	186995	2	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130230	171879	2	None	3	2	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL448085	171879	2	None	3	2	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	1269	121	33	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	-56	6	Rat	5.6	pEC50	=	5.6	Functional	Agonist activity at rat histamine H4 receptorAgonist activity at rat histamine H4 receptor	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	44480958	5582	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccncc1	10.1021/jm900526h
	CHEMBL1077859	5582	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccncc1	10.1021/jm900526h
	25131556	188274	7	None	3	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL507063	188274	7	None	3	2	Human	7.6	pEC50	=	7.6	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	127051047	139768	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806162	139768	0	None	-	1	Human	5.6	pEC50	=	5.6	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	45257134	198251	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	CHEMBL594949	198251	0	None	-	1	Human	6.6	pEC50	=	6.6	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	25131231	186594	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(I)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492288	186594	0	None	-	1	Rat	6.6	pEC50	=	6.6	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(I)cc3)nc(N)n2)CC1	10.1021/jm8005959
	10381810	203453	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL7828	203453	0	None	-	1	Human	7.6	pEC50	=	7.6	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	155512725	175657	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175657	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175657	0	None	1	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	127052294	139759	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139759	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1893	114	None	-97	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-97	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-97	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-97	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-97	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-97	11	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25128822	186888	0	None	-19	2	Human	6.5	pEC50	=	6.5	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL494093	186888	0	None	-19	2	Human	6.5	pEC50	=	6.5	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	155491015	175911	3	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175911	3	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175911	3	None	-31	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assayAgonist activity at human H4R expressed on HEK293T cells assessed as inhibition of forskolin-induced CRE-driven luciferase activity co-incubated with forskolin for 6 hrs and measured after 30 mins by CRE-luciferase reporter gene assay	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	46890072	6817	0	None	-	1	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084355	6817	0	None	-	1	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7220	0	None	3	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7220	0	None	3	4	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	58227373	175924	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175924	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175924	0	None	1	3	Human	8.5	pEC50	=	8.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	21638785	188063	4	None	-1	2	Rat	7.5	pEC50	=	7.5	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188063	4	None	-1	2	Rat	7.5	pEC50	=	7.5	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	25130911	184637	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	236	2	1	6	-0.1	CN1CCN(c2cc(N(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL485802	184637	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	236	2	1	6	-0.1	CN1CCN(c2cc(N(C)C)nc(N)n2)CC1	10.1021/jm8005959
	16352	116670	11	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL339414	116670	11	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	1267	3732	43	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-8	5	Mouse	6.5	pEC50	=	6.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	25130579	187119	2	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	238	4	1	5	1.3	CN(C)CCOc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL495341	187119	2	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	238	4	1	5	1.3	CN(C)CCOc1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	10678661	5275	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106931	5275	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	46228101	198106	0	None	-	1	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594022	198106	0	None	-	1	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	1249	1970	14	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	13499360	1970	14	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90019	1970	14	None	-18	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1269	121	33	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	35	6	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	25129528	186593	0	None	-	1	Rat	7.5	pEC50	=	7.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(Cl)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492287	186593	0	None	-	1	Rat	7.5	pEC50	=	7.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(Cl)c3)nc(N)n2)CC1	10.1021/jm8005959
	44568923	192491	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	327	2	1	5	2.3	CN1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL522661	192491	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	327	2	1	5	2.3	CN1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)CC1	10.1021/jm8005959
	67087450	116621	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	116621	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	44547127	115405	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115405	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227148	116618	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	116618	0	None	-	1	Human	5.5	pEC50	=	5.5	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	10678661	5275	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106931	5275	1	None	-309	2	Human	4.5	pEC50	=	4.5	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	25131557	192646	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	8	0.3	COc1ncc(-c2cc(N3CCN(C)CC3)nc(N)n2)cn1	10.1021/jm8005959
	CHEMBL523835	192646	0	None	-	1	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	8	0.3	COc1ncc(-c2cc(N3CCN(C)CC3)nc(N)n2)cn1	10.1021/jm8005959
	25130909	186787	0	None	1	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	CHEMBL493478	186787	0	None	1	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	44478385	5684	2	None	20	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078642	5684	2	None	20	2	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	46890549	6492	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083085	6492	0	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44480960	5539	2	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	248	6	3	3	1.2	CC(C)N/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077636	5539	2	None	-	1	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	248	6	3	3	1.2	CC(C)N/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	42625303	180174	0	None	-2089	7	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180174	0	None	-2089	7	Rat	5.5	pEC50	=	5.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	56954425	81655	0	None	-16	3	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	584	23	6	4	4.0	N/C(=N\C(=O)CCCCCCCCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165643	81655	0	None	-16	3	Human	6.5	pEC50	=	6.5	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	584	23	6	4	4.0	N/C(=N\C(=O)CCCCCCCCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	25130913	169609	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	278	2	1	7	-0.4	CN1CCN(c2cc(N3CCOCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL444484	169609	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	278	2	1	7	-0.4	CN1CCN(c2cc(N3CCOCC3)nc(N)n2)CC1	10.1021/jm8005959
	1274	3989	12	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	3989	12	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	3989	12	None	-776	4	Rat	4.5	pEC50	=	4.5	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	155517880	176083	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	-3	3	Human	7.5	pEC50	=	7.5	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	175894	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175894	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175894	0	None	-8	3	Mouse	7.5	pEC50	=	7.5	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	25129180	186658	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	4	1	6	0.7	CCN(CC)c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL492662	186658	0	None	-	1	Rat	6.5	pEC50	=	6.5	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	4	1	6	0.7	CCN(CC)c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	3228743	186788	5	None	-	1	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL493479	186788	5	None	-	1	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	12769844	108977	0	None	-3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	234	5	3	3	0.7	CN/C(=N/C#N)NCCCCc1nc[nH]c1C	10.1039/C3MD00245D
	CHEMBL3220641	108977	0	None	-3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	234	5	3	3	0.7	CN/C(=N/C#N)NCCCCc1nc[nH]c1C	10.1039/C3MD00245D
	25129181	186659	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	305	3	1	7	-0.0	CN1CCN(c2cc(N3CCC(N(C)C)C3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492663	186659	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	305	3	1	7	-0.0	CN1CCN(c2cc(N3CCC(N(C)C)C3)nc(N)n2)CC1	10.1021/jm8005959
	25128825	186971	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.2	CN1CCC(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494585	186971	0	None	-	1	Rat	5.4	pEC50	=	5.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	248	1	1	4	2.2	CN1CCC(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	44478385	5684	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/acs.jmedchem.6b00120
	CHEMBL1078642	5684	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/acs.jmedchem.6b00120
	44478385	5684	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1039/C3MD00245D
	CHEMBL1078642	5684	2	None	20	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1039/C3MD00245D
	25129527	186821	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493720	186821	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm8005959
	24798596	198175	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594491	198175	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	127052292	139723	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805663	139723	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	350	7	3	3	2.9	N#C/N=C(/NCCCc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1274	3989	12	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	3989	12	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	3989	12	None	7	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	11846465	141388	0	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	CHEMBL387054	141388	0	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	25131559	186992	0	None	-23	2	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494667	186992	0	None	-23	2	Rat	6.4	pEC50	=	6.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	2	6	0.2	CN1CCN(c2cc(-c3c[nH]cn3)nc(N)n2)CC1	10.1021/jm8005959
	25131561	186811	0	None	3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL493664	186811	0	None	3	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	286	2	1	7	-0.0	CN1CCN(c2cc(-n3ccccc3=O)nc(N)n2)CC1	10.1021/jm8005959
	46235428	6511	0	None	-263	3	Mouse	7.4	pEC50	=	7.4	Functional	Intrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6511	0	None	-263	3	Mouse	7.4	pEC50	=	7.4	Functional	Intrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at mouse histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1269	121	33	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	35	6	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	136030655	56173	0	None	-3	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56173	0	None	-3	3	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25129523	186694	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492884	186694	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25129523	186694	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	186694	40	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	70692530	75324	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL2047474	75324	0	None	-	1	Human	6.4	pEC50	=	6.4	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	91613	17789	31	None	-5	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL12620	17789	31	None	-5	2	Human	5.4	pEC50	=	5.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	58227326	116613	0	None	-	1	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393542	116613	0	None	-	1	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	58227373	175924	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175924	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175924	0	None	-3	3	Mouse	8.4	pEC50	=	8.4	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	25071004	192106	0	None	1	2	Mouse	8.4	pEC50	=	8.4	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192106	0	None	1	2	Mouse	8.4	pEC50	=	8.4	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	42625303	180174	0	None	-2	7	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180174	0	None	-2	7	Human	8.4	pEC50	=	8.4	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	25128824	192565	0	None	1	2	Mouse	8.3	pEC50	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192565	0	None	1	2	Mouse	8.3	pEC50	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	10702196	108068	1	None	-1	2	Human	4.4	pEC50	=	4.4	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL320279	108068	1	None	-1	2	Human	4.4	pEC50	=	4.4	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	56954320	81653	0	None	-12	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	472	15	6	4	0.9	N/C(=N\C(=O)CCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165641	81653	0	None	-12	4	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	472	15	6	4	0.9	N/C(=N\C(=O)CCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	1267	3732	43	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	3035905	3732	43	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	CHEMBL260374	3732	43	None	-17	5	Human	6.4	pEC50	=	6.4	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2015.12.035
	25129524	186591	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	CHEMBL492274	186591	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1cccc(-c2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1021/jm8005959
	25130909	186787	0	None	-1	2	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	CHEMBL493478	186787	0	None	-1	2	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2nc(N)ncc2N)CC1	10.1021/jm8005959
	1278	2110	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2110	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2110	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2110	79	None	-1	4	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	25128833	187808	30	None	2	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	CHEMBL501882	187808	30	None	2	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1021/jm8005959
	25129526	186820	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL493719	186820	0	None	-	1	Rat	7.4	pEC50	=	7.4	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	299	3	1	6	1.5	COc1ccccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	24797248	198174	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594490	198174	2	None	-	1	Human	7.4	pEC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	127050434	139707	0	None	3	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	160	3	4	3	0.2	CC(=N)SCCNC(=N)N	10.1021/acs.jmedchem.6b00120
	CHEMBL3805514	139707	0	None	3	2	Human	7.4	pEC50	=	7.4	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	160	3	4	3	0.2	CC(=N)SCCNC(=N)N	10.1021/acs.jmedchem.6b00120
	25129169	186588	0	None	1	2	Mouse	7.3	pEC50	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186588	0	None	1	2	Mouse	7.3	pEC50	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	24798595	169797	2	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	276	2	1	6	0.8	CN1CCN(c2cc(N3CCCCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL444782	169797	2	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	276	2	1	6	0.8	CN1CCN(c2cc(N3CCCCC3)nc(N)n2)CC1	10.1021/jm8005959
	90667239	108976	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	8	3	4	2.5	Cc1nc(CCCN/C(=N\C#N)NCCSc2ccccc2)c[nH]1	10.1039/C3MD00245D
	CHEMBL3220640	108976	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	342	8	3	4	2.5	Cc1nc(CCCN/C(=N\C#N)NCCSc2ccccc2)c[nH]1	10.1039/C3MD00245D
	25130573	186292	2	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL489663	186292	2	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(N)nc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130581	186632	0	None	-3	2	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL492489	186632	0	None	-3	2	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	264	2	0	5	1.5	COc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	25130235	186994	1	None	-4	3	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	186994	1	None	-4	3	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein by GTPgammaS assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	46890546	6422	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6422	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228143	198390	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595880	198390	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	117724523	116600	2	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393529	116600	2	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	25130914	186661	0	None	-15	2	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492677	186661	0	None	-15	2	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25129179	187075	4	None	-	1	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	222	2	2	6	-0.1	CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL495112	187075	4	None	-	1	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	222	2	2	6	-0.1	CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25130236	187108	0	None	1	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	CHEMBL495265	187108	0	None	1	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	25068753	187844	15	None	1	2	Mouse	8.2	pEC50	=	8.2	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187844	15	None	1	2	Mouse	8.2	pEC50	=	8.2	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	53233805	61180	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770964	61180	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	46890545	7289	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086460	7289	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1274	3989	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	3063228	3989	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	CHEMBL1196470	3989	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	1274	3989	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	3063228	3989	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	CHEMBL1196470	3989	12	None	7	4	Human	7.3	pEC50	=	7.3	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activityAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-induced cAMP-mediated CRE-beta galactosidase activity	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	136141537	56172	1	None	-5	3	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56172	1	None	-5	3	Rat	6.3	pEC50	=	6.3	Functional	Agonist activity at rat histamine H4 receptor by luciferase reporter gene assayAgonist activity at rat histamine H4 receptor by luciferase reporter gene assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	11313837	2454	7	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1254	2454	7	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL175782	2454	7	None	-15848	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	10583206	108859	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321920	108859	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-51	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-51	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-51	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-51	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-51	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-51	11	Mouse	5.3	pEC50	=	5.3	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	155513612	175736	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-18	3	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155512725	175657	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175657	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175657	0	None	-6	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	58227163	175879	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175879	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175879	0	None	-4	3	Mouse	7.3	pEC50	=	7.3	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25130580	173720	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.6	CNc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	CHEMBL455016	173720	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	263	2	1	5	1.6	CNc1nc(N2CCN(C)CC2)cc(C(C)(C)C)n1	10.1021/jm8005959
	25129878	186742	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	324	3	1	7	1.4	COc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL493103	186742	0	None	-	1	Rat	5.3	pEC50	=	5.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	324	3	1	7	1.4	COc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	127052295	139654	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	352	7	3	4	2.4	N#C/N=C(/NCCOc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804862	139654	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	352	7	3	4	2.4	N#C/N=C(/NCCOc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1893	114	None	-97	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-97	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-97	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-97	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-97	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-97	11	Rat	4.3	pEC50	=	4.3	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	53233803	61178	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770962	61178	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	25129169	186588	0	None	-1	2	Rat	7.3	pEC50	=	7.3	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186588	0	None	-1	2	Rat	7.3	pEC50	=	7.3	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	127049856	139702	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805431	139702	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assayAgonist activity at human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 90 mins by [35S]GTPgammaS binding assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	90667238	108975	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	220	4	3	3	0.3	CN/C(=N/C#N)NCCCc1c[nH]c(C)n1	10.1039/C3MD00245D
	CHEMBL3220639	108975	0	None	-	1	Human	6.3	pEC50	=	6.3	Functional	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation countingAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 assessed as [35S]GTPgammaS binding after 90 mins by liquid scintillation counting	ChEMBL	220	4	3	3	0.3	CN/C(=N/C#N)NCCCc1c[nH]c(C)n1	10.1039/C3MD00245D
	565544	109242	9	None	-4466	2	Human	6.3	pEC50	=	6.3	Functional	Inhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptor	ChEMBL	125	2	2	2	0.5	CC(CN)c1cnc[nH]1	10.1021/jm0300025
	CHEMBL322988	109242	9	None	-4466	2	Human	6.3	pEC50	=	6.3	Functional	Inhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production for human Histamine H4 receptor	ChEMBL	125	2	2	2	0.5	CC(CN)c1cnc[nH]1	10.1021/jm0300025
	10583206	108859	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321920	108859	0	None	-6	2	Human	5.3	pEC50	=	5.3	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	25130915	186695	0	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL492885	186695	0	None	-	1	Rat	7.3	pEC50	=	7.3	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	283	2	1	5	1.8	Cc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1021/jm8005959
	46228125	198295	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595215	198295	0	None	-	1	Human	7.3	pEC50	=	7.3	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	44482028	5533	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccn1	10.1021/jm900526h
	CHEMBL1077580	5533	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	325	9	3	4	1.8	N#C/N=C(/NCCCCc1c[nH]cn1)NCCCc1ccccn1	10.1021/jm900526h
	25131232	186697	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	308	2	1	6	1.7	Cc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL492900	186697	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	308	2	1	6	1.7	Cc1c(-c2ccc(C#N)cc2)nc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	25130235	186994	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	186994	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	186994	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	186994	1	None	-4	3	Rat	7.2	pEC50	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	45257133	198212	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198212	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25128824	192565	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192565	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	155517880	176083	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	-2	3	Mouse	8.2	pEC50	=	8.2	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	45269820	193669	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202326	193669	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL551790	193669	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assayAgonist activity at histamine H4 receptor (unknown origin) expressed in HEK293T cells harboring CRE-luciferase after 6 hrs by luciferase reporter gene assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	25071004	192106	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192106	0	None	-1	2	Rat	8.2	pEC50	=	8.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	46890022	6953	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	8	3	4	4.3	Cc1cc(OCCCNCC2CCCNC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084869	6953	0	None	-	1	Human	8.2	pEC50	=	8.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	410	8	3	4	4.3	Cc1cc(OCCCNCC2CCCNC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228158	200463	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL609289	200463	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890547	6423	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082772	6423	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	1255	3134	11	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	6421522	3134	11	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL19173	3134	11	None	-19	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	9974306	202759	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72372	202759	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	10060212	202819	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72769	202819	0	None	-	1	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	136192874	56174	0	None	1	3	Mouse	7.2	pEC50	=	7.2	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56174	0	None	1	3	Mouse	7.2	pEC50	=	7.2	Functional	Agonist activity at mouse histamine H4 receptor by luciferase reporter gene assayAgonist activity at mouse histamine H4 receptor by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1236	2028	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1237	2028	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	156615	2028	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL268229	2028	23	None	-549	3	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	25129175	186786	0	None	-	1	Rat	6.2	pEC50	=	6.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL493477	186786	0	None	-	1	Rat	6.2	pEC50	=	6.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	223	2	1	6	-0.2	COc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	25130914	186661	0	None	15	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492677	186661	0	None	15	2	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at human histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25130908	171275	2	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	CHEMBL446896	171275	2	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	207	1	1	5	0.1	Cc1cnc(N)nc1N1CCN(C)CC1	10.1021/jm8005959
	24797775	186652	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492631	186652	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2cc(C(C)(C)C)nc(N)n2)CC1	10.1021/jm8005959
	1204	1893	114	None	-51	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-51	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-51	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-51	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-51	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-51	11	Mouse	5.2	pEC50	=	5.2	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25130912	188143	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL505061	188143	0	None	-	1	Rat	7.2	pEC50	=	7.2	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1021/jm8005959
	1204	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R by [35S]-GTPgammaS-binding assayAgonist activity at human H4R by [35S]-GTPgammaS-binding assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	25149521	178464	0	None	1	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL471413	178464	0	None	1	3	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1247	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1375	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	774	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	CHEMBL90	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	DB05381	1893	114	None	-1	11	Human	8.1	pEC50	=	8.1	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2015.12.035
	1267	3732	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3732	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3732	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	1269	121	33	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	37463	121	33	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	CHEMBL275443	121	33	None	35	6	Human	7.2	pEC50	=	7.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1021/jm900526h
	1267	3732	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3732	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3732	43	None	-17	5	Human	7.2	pEC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingInverse agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	44482031	5551	1	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	234	6	3	3	0.8	CCN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077756	5551	1	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	234	6	3	3	0.8	CCN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	46890019	7216	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7216	0	None	-	1	Human	6.2	pEC50	=	6.2	Functional	Agonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAgonist activity at human histamine H4 receptor in SK-N-MC cells assessed as forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	156015638	177687	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177687	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177687	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	1279	2110	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	4908365	2110	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	CHEMBL129198	2110	79	None	-1	4	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat H4R by [35S]-GTPgammaS-binding assayAgonist activity at rat H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	10978141	5066	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL105803	5066	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	58227127	116614	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393543	116614	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	44481489	5622	0	None	18	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(\NCCCc1ccccc1)NCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1078210	5622	0	None	18	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	310	8	3	3	2.0	N#C/N=C(\NCCCc1ccccc1)NCCCc1c[nH]cn1	10.1021/jm900526h
	10419556	5271	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106913	5271	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	11086114	7298	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL108649	7298	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10820930	5707	0	None	-	1	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL107877	5707	0	None	-	1	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10106063	108770	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321860	108770	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10106063	108770	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	CHEMBL321860	108770	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP production incubated for 6 hrs by CRE/beta-galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	56954318	81652	0	None	-7	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	444	13	6	4	0.1	N/C(=N\C(=O)CCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165640	81652	0	None	-7	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	444	13	6	4	0.1	N/C(=N\C(=O)CCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	10106063	108770	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321860	108770	6	None	40	2	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10017000	202770	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72431	202770	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-1	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-1	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-1	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-1	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-1	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-1	11	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	136192874	56174	0	None	-1	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56174	0	None	-1	3	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assayAgonist activity at human recombinant histamine H4 receptor expressed in human SK-N-MC cells co-expressing SRE-Luc by luciferase reporter gene assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1267	3732	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	3035905	3732	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	CHEMBL260374	3732	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assayInverse agonist activity at human histamine H4 receptor expressed in HEK293 cells assessed by forskolin induced cAMP response element activation by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	1239	1994	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1240	1994	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	912	1994	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL268490	1994	23	None	-1995	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10978141	5066	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL105803	5066	1	None	-7	2	Human	6.1	pEC50	=	6.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	1278	2110	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	1279	2110	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	4908365	2110	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	CHEMBL129198	2110	79	None	-1	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.9b01342
	42625303	180174	0	None	-501	7	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	CHEMBL475621	180174	0	None	-501	7	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/acs.jmedchem.9b01342
	10419556	5271	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106913	5271	0	None	-9	2	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	11086114	7298	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL108649	7298	0	None	-	1	Human	5.1	pEC50	=	5.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assayAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells by CRE-beta galactosidase reporter gene assay	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	155513612	175736	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-14	3	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R expressed in Sf9 cells by [35S]-GTPgammaS-binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	25130230	171879	2	None	-3	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL448085	171879	2	None	-3	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	259	2	1	7	-0.0	CN1CCN(c2cc(-n3ccnc3)nc(N)n2)CC1	10.1021/jm8005959
	25133971	186756	2	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2nc(N)cc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	CHEMBL493261	186756	2	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	249	1	1	5	1.1	CN1CCN(c2nc(N)cc(C(C)(C)C)n2)CC1	10.1021/jm8005959
	25130229	188159	6	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	271	2	1	7	0.3	CN1CCN(c2cc(-c3cncnc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL505346	188159	6	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	271	2	1	7	0.3	CN1CCN(c2cc(-c3cncnc3)nc(N)n2)CC1	10.1021/jm8005959
	56954515	81639	0	None	-16	4	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	546	17	6	6	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165628	81639	0	None	-16	4	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	546	17	6	6	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm201128q
	56954322	81654	0	None	-10	4	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	500	17	6	4	1.7	N/C(=N\C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	CHEMBL2165642	81654	0	None	-10	4	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assayAgonist activity at human H4R-RGS19 Galphai2 Gbeta1gamma2 expressed in Sf9 cells at 0.1 nM to 1 mM by steady state GTPase activity assay	ChEMBL	500	17	6	4	1.7	N/C(=N\C(=O)CCCCCCCCC(=O)/N=C(\N)NCCCc1c[nH]cn1)NCCCc1c[nH]cn1	10.1021/jm201128q
	45257131	198216	0	None	-	1	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198216	0	None	-	1	Human	8.1	pEC50	=	8.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS bindingAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of [35S]GTPgammaS binding	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	11846298	138738	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	CHEMBL379102	138738	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Activity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assayActivity at human histamine H4 receptor transfected in SK-N-MC cells by cAMP assay	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	25129176	172182	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2nc(N)ncc2-c2ccccc2)CC1	10.1021/jm8005959
	CHEMBL451108	172182	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	269	2	1	5	1.5	CN1CCN(c2nc(N)ncc2-c2ccccc2)CC1	10.1021/jm8005959
	58227388	116609	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393538	116609	1	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	1223	938	32	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	2790	938	32	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	CHEMBL14690	938	32	None	-812	4	Mouse	6.1	pEC50	=	6.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.9b01342
	10820929	107708	1	None	-30	2	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL319658	107708	1	None	-30	2	Human	4.1	pEC50	=	4.1	Functional	Inhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptorInhibition of the forskolin-stimulated cAMP production in SK-N-MC cells expressing human Histamine H4 receptor	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	53233801	61201	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770985	61201	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrsAgonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as effect on forskolin-induced cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	58227252	116615	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393544	116615	0	None	-	1	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	1204	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-51	11	Mouse	7.1	pEC50	=	7.1	Functional	Agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1274	3989	12	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	3063228	3989	12	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	CHEMBL1196470	3989	12	None	-199	4	Mouse	5.1	pEC50	=	5.1	Functional	Agonist activity at mouse H4R by [35S]-GTPgammaS-binding assayAgonist activity at mouse H4R by [35S]-GTPgammaS-binding assay	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/acs.jmedchem.9b01342
	25130236	187108	0	None	-1	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	CHEMBL495265	187108	0	None	-1	2	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	268	1	0	4	2.2	CN1CCN(c2cc(C(C)(C)C)nc(Cl)n2)CC1	10.1021/jm8005959
	1267	3732	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3732	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3732	43	None	-17	5	Human	7.1	pEC50	=	7.1	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	25129877	168882	0	None	-	1	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(I)c3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL442865	168882	0	None	-	1	Rat	6.1	pEC50	=	6.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	395	2	1	5	2.1	CN1CCN(c2cc(-c3cccc(I)c3)nc(N)n2)CC1	10.1021/jm8005959
	44568847	186656	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	6	2.0	CN1CCN(c2cc(Sc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL492652	186656	0	None	-	1	Rat	5.1	pEC50	=	5.1	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	301	3	1	6	2.0	CN1CCN(c2cc(Sc3ccccc3)nc(N)n2)CC1	10.1021/jm8005959
	25101945	192494	0	None	1	2	Mouse	8.0	pEC50	=	8.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192494	0	None	1	2	Mouse	8.0	pEC50	=	8.0	Functional	Antagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at mouse histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	25101945	192494	0	None	-1	2	Rat	8.0	pEC50	=	8.0	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192494	0	None	-1	2	Rat	8.0	pEC50	=	8.0	Functional	Antagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assayAntagonist activity at rat histamine H4 receptor expressed in HEK293 cells assessed as inhibition of histamine-induced intracellular calcium elevation by FLIPR assay	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	58227143	116612	1	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393541	116612	1	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activityAgonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as effect on forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	16061961	197987	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197987	0	None	-	1	Human	7.0	pEC50	=	7.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 assessed as stimulation of GTPase by [gamma-32P]GTP spontaneous degradation assay	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	112936251	136729	1	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752085	136729	1	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	58227163	175879	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175879	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175879	0	None	-8	3	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assayAgonist activity at rat H4R expressed in HEK293-SF-rH4R-His6-CRE-Luc cells incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25131556	188274	7	None	-3	2	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	CHEMBL507063	188274	7	None	-3	2	Rat	7.0	pEC50	=	7.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	300	3	1	7	0.9	COc1ncccc1-c1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm8005959
	25128830	187120	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	234	1	0	4	1.5	CN1CCN(c2cc(C(C)(C)C)ncn2)CC1	10.1021/jm8005959
	CHEMBL495342	187120	0	None	-	1	Rat	6.0	pEC50	=	6.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	234	1	0	4	1.5	CN1CCN(c2cc(C(C)(C)C)ncn2)CC1	10.1021/jm8005959
	127035835	136748	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752270	136748	0	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Antagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation countingAntagonist activity at human histamine 4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 assessed as [35S]GTPgammaS binding by scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	11983344	5586	5	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	220	5	3	3	0.4	CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	CHEMBL1077862	5586	5	None	-	1	Human	6.0	pEC50	=	6.0	Functional	Agonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assayAgonist activity at human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19 protein by steady-state GTPase assay	ChEMBL	220	5	3	3	0.4	CN/C(=N\C#N)NCCCCc1c[nH]cn1	10.1021/jm900526h
	25128829	186741	0	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	265	5	1	5	1.7	CN(C)CCCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	CHEMBL493100	186741	0	None	-	1	Rat	5.0	pEC50	=	5.0	Functional	Agonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assayAgonist activity at rat histamine H4 receptor expressed in HEK293 cells coexpressing Gqi5 protein assessed as calcium flux by FLIPR assay	ChEMBL	265	5	1	5	1.7	CN(C)CCCN(C)c1cc(C(C)(C)C)nc(N)n1	10.1021/jm8005959
	24745335	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	9.2	pIC50	=	9.2	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	8.9	pIC50	=	8.9	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	156013896	177655	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-3	3	Human	8.8	pIC50	=	8.8	Functional	Inverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293-SF-hH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	44129134	197817	0	None	-	1	Human	8.0	pIC50	=	8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	197817	0	None	-	1	Human	8.0	pIC50	=	8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	56835171	69382	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69382	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	16082828	79835	0	None	-6	3	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213886	79835	0	None	-6	3	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	71525678	89281	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89281	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	71525679	89282	0	None	-	1	Human	6.9	pIC50	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89282	0	None	-	1	Human	6.9	pIC50	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	1278	2110	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	1279	2110	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	4908365	2110	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	CHEMBL129198	2110	79	None	1	4	Human	7.9	pIC50	=	7.9	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2019.02.020
	10446295	2092	29	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2092	29	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2092	29	None	11	2	Human	6.9	pIC50	=	6.9	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	155563181	174721	0	None	-	1	Human	5.9	pIC50	=	5.9	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	498	12	1	7	5.1	CCOC(=O)C1=C(C)NC(C)=C(C(=O)OCC)C1c1ccc(OCCCCCN2CCCCC2)cc1	10.1021/acs.jmedchem.9b00937
	CHEMBL4573309	174721	0	None	-	1	Human	5.9	pIC50	=	5.9	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	498	12	1	7	5.1	CCOC(=O)C1=C(C)NC(C)=C(C(=O)OCC)C1c1ccc(OCCCCCN2CCCCC2)cc1	10.1021/acs.jmedchem.9b00937
	25147771	173656	0	None	-	1	Human	6.8	pIC50	=	6.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	173656	0	None	-	1	Human	6.8	pIC50	=	6.8	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	16082833	79922	0	None	-40	2	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214312	79922	0	None	-40	2	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	90645145	111673	0	None	-	1	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290578	111673	0	None	-	1	Human	5.8	pIC50	=	5.8	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1267	3732	43	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	3035905	3732	43	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	CHEMBL260374	3732	43	None	-17	5	Human	7.7	pIC50	=	7.7	Functional	Antagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assayAntagonist activity at human H4 receptor expressed in CHO-K1 cells co-expressing G protein alpha16 assessed as inhibition of histamine-induced calcium mobilization incubated for 16 hrs by aequorin-based functional assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.9b00937
	1278	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1278	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	1279	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	4908365	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	CHEMBL129198	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assayAntagonist actiivty at human H4R expressed in human U2OS cells assessed as inhibition of [35S]GTPgammaS binding after 30 mins by scintillation proximity assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	3201696	15578	3	None	-22	2	Human	4.7	pIC50	=	4.7	Functional	Antagonist activity at human histamine H4 receptorAntagonist activity at human histamine H4 receptor	ChEMBL	348	5	0	6	2.1	CN1CCN(C(c2ccccc2)c2nnnn2Cc2ccccc2)CC1	10.1016/j.bmcl.2010.07.009
	CHEMBL1222728	15578	3	None	-22	2	Human	4.7	pIC50	=	4.7	Functional	Antagonist activity at human histamine H4 receptorAntagonist activity at human histamine H4 receptor	ChEMBL	348	5	0	6	2.1	CN1CCN(C(c2ccccc2)c2nnnn2Cc2ccccc2)CC1	10.1016/j.bmcl.2010.07.009
	1278	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	1	4	Human	6.7	pIC50	=	6.7	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	63868328	173669	2	None	-	1	Human	6.6	pIC50	=	6.6	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4549015	173669	2	None	-	1	Human	6.6	pIC50	=	6.6	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71548412	1870	14	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1870	14	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1870	14	None	14	2	Human	7.6	pIC50	=	7.6	Functional	Antagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant human histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	16656871	7879	0	None	-	1	Human	6.5	pIC50	=	6.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090531	7879	0	None	-	1	Human	6.5	pIC50	=	6.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	16718567	8063	0	None	3311	2	Human	8.5	pIC50	=	8.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091874	8063	0	None	3311	2	Human	8.5	pIC50	=	8.5	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2110	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	1	4	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assayAntagonist activity at H4R in human eosinophils assessed as inhibition of imetit-induced shape change by GAFS assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	155540438	171964	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4483783	171964	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Antagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assayAntagonist activity at recombinant human H4 receptor expressed in CHOK1 cells co-expressing Galpha16/aequorin assessed as inhibition of histamine-induced intracellular calcium flux preincubated for 30 mins followed by histamine addition and measured for 30 secs in presence of coelenterazine by luminescence assay	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	44129130	197865	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	197865	0	None	-	1	Human	7.5	pIC50	=	7.5	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11682208	8175	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092542	8175	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	25112105	8064	0	None	-	1	Human	8.4	pIC50	=	8.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091875	8064	0	None	-	1	Human	8.4	pIC50	=	8.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	24745335	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced CD11b upregulation	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	1278	2110	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2110	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2110	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2110	79	None	1	4	Human	7.4	pIC50	=	7.4	Functional	Inhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptorInhibition of mouse bone-marrow mast cell chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	16656872	7815	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090190	7815	0	None	-	1	Human	7.4	pIC50	=	7.4	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	122193095	123383	0	None	-	1	Human	6.4	pIC50	=	6.4	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123383	0	None	-	1	Human	6.4	pIC50	=	6.4	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	49841846	69380	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69380	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	49841719	69379	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69379	0	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	1278	2110	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	1	4	Human	8.3	pIC50	=	8.3	Functional	Antagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisationAntagonist activity at H4R in human eosinophils assessed as inhibition of histamine-induced actin polymerisation	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	90645147	111671	0	None	-	1	Human	6.3	pIC50	=	6.3	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290576	111671	0	None	-	1	Human	6.3	pIC50	=	6.3	Functional	Antagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassayAntagonist activity at human histamine H4 receptor expressed in gamma-irradiated recombinant CHO cells assessed as inhibition of forskolin/histamine-induced intracellular cAMP accumulation preincubated for 30 mins followed by Eu chelate-labeled cAMP/anti-cAMP mAb addition measured after 1 hr by TR-FRET immunoassay	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	24745335	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	-	1	Human	7.3	pIC50	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	10446295	2092	29	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2092	29	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2092	29	None	11	2	Human	6.3	pIC50	=	6.3	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	122194625	123490	0	None	-	1	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629828	123490	0	None	-	1	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassayAntagonist activity at human H4R expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 30 mins by TR-FRET immunoassay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	1267	3732	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	3035905	3732	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	CHEMBL260374	3732	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assayAntagonist activity at histamine H4 receptor expressed in HEK293 cells co-transfected with pCRE-Luc gene assessed as inhibition of forskolin/histamine-induced cAMP accumulation after 5 hrs by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.09.061
	1267	3732	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3035905	3732	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	CHEMBL260374	3732	43	None	-17	5	Human	6.2	pIC50	=	6.2	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	25147772	172827	4	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	172827	4	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assayInverse agonist activity at human histamine H4 receptor expressed in HEK293T cells by CRE-beta-galactosidase assay	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	71548412	1870	14	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1870	14	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1870	14	None	-14	2	Mouse	6.2	pIC50	=	6.2	Functional	Antagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assayAntagonist activity at recombinant mouse histamine H4 receptor expressed in CHO-K1 cells assessed as inhibition of R-alpha-methylhistamine induced stimulation of [35S]-GTPgammaS binding by scintillation proximity assay	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	11709443	8147	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092332	8147	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71519674	89285	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89285	0	None	-	1	Human	7.2	pIC50	=	7.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	16656874	8007	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091569	8007	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assayInverse agonist activity at human histamine H4 receptor assessed as inhibition of [35S]GTPgammaS binding after 15 mins by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	49841845	69381	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69381	0	None	-	1	Human	7.1	pIC50	=	7.1	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	156013896	177655	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-38	3	Mouse	7.1	pIC50	=	7.1	Functional	Inverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assayInverse agonist activity at mouse H4R expressed in HEK293-SF-mH4R-His6-CRE-Luc cells assessed as reduction in histamine-induced inhibition of forskolin stimulated luciferase activity by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2110	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2110	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2110	79	None	1	4	Human	7.1	pIC50	=	7.1	Functional	Inhibition of human eosinophil chemotaxis mediated by histamine H4 receptorInhibition of human eosinophil chemotaxis mediated by histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	16082831	79921	0	None	-100	3	Human	6.1	pIC50	=	6.1	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL214311	79921	0	None	-100	3	Human	6.1	pIC50	=	6.1	Functional	Antagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assayAntagonist activity at human H4 receptor expressed in 293-EBNA cells assessed as inhibition of histamine agonist activity by luciferase reporter gene assay	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	71525681	89284	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89284	0	None	-	1	Human	7.0	pIC50	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human histamine H4 receptor expressed in HEK cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	1267	3732	43	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3732	43	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3732	43	None	-17	5	Human	6.0	pIC50	=	6.0	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	58227148	116618	0	None	-	1	Human	10.1	pKd	=	10.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	116618	0	None	-	1	Human	10.1	pKd	=	10.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	59777258	109631	0	None	-	0	Human	10.0	pKd	=	10	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236561	109631	0	None	-	0	Human	10.0	pKd	=	10	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	24797604	109622	2	None	-	0	Human	9.9	pKd	=	9.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	CHEMBL3236552	109622	2	None	-	0	Human	9.9	pKd	=	9.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	24994542	109624	0	None	-	0	Human	9.6	pKd	=	9.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236554	109624	0	None	-	0	Human	9.6	pKd	=	9.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	24798144	109623	2	None	-	1	Human	9.5	pKd	=	9.5	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109623	2	None	-	1	Human	9.5	pKd	=	9.5	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	53257796	109645	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236575	109645	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	54587790	61196	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770980	61196	0	None	-	0	Human	9.4	pKd	=	9.4	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	24745337	61195	0	None	-	0	Human	9.3	pKd	=	9.3	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770979	61195	0	None	-	0	Human	9.3	pKd	=	9.3	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	59777338	109626	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236556	109626	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	44547268	116597	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393526	116597	0	None	-	0	Human	9.2	pKd	=	9.2	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227340	116603	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393532	116603	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	58227200	116606	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393535	116606	0	None	-	0	Human	9.0	pKd	=	9	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	54585781	61217	0	None	-	0	Human	8.9	pKd	=	8.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771000	61217	0	None	-	0	Human	8.9	pKd	=	8.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	54583873	61185	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770969	61185	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54587787	61187	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770971	61187	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54581940	61208	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770992	61208	0	None	-	0	Human	8.8	pKd	=	8.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25130912	188143	0	None	-	1	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL505061	188143	0	None	-	1	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	23653543	67691	2	None	-	0	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914541	67691	2	None	-	0	Human	8.7	pKd	=	8.7	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	24994636	109635	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	CHEMBL3236565	109635	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	42624149	109638	1	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109638	1	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	59777262	109642	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236572	109642	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	54584824	61193	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770977	61193	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227337	116624	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393553	116624	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	58227259	116627	0	None	-	1	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	116627	0	None	-	1	Human	8.0	pKd	=	8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	24798144	109623	2	None	-	1	Mouse	8.0	pKd	=	8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109623	2	None	-	1	Mouse	8.0	pKd	=	8	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	24826766	67744	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914750	67744	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	9944571	116503	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338937	116503	0	None	-	0	Human	8.0	pKd	=	8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	24994634	2102	26	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2102	26	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2102	26	None	-	1	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	53258370	109639	1	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236569	109639	1	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	24994635	109640	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236570	109640	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	54582865	61175	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770959	61175	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	54584825	61206	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770990	61206	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	11587279	124518	3	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL364400	124518	3	None	-	0	Human	7.9	pKd	=	7.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	46890627	6496	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083098	6496	0	None	-	0	Human	7.9	pKd	=	7.9	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740470	109297	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3233048	109297	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	11687917	14781	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	CHEMBL120854	14781	0	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	9966927	18672	4	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128252	18672	4	None	-	0	Human	6.9	pKd	=	6.9	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	54581938	61188	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770972	61188	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	67087450	116621	0	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	116621	0	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	11594278	14506	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL120591	14506	0	None	-	0	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	9923162	18736	5	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128613	18736	5	None	-	1	Human	7.8	pKd	=	7.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	11120826	18715	1	None	-	0	Human	6.8	pKd	=	6.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128505	18715	1	None	-	0	Human	6.8	pKd	=	6.8	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	46890677	7195	0	None	-	0	Human	6.8	pKd	=	6.8	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086038	7195	0	None	-	0	Human	6.8	pKd	=	6.8	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7220	0	None	-3	4	Mouse	4.8	pKd	=	4.8	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7220	0	None	-3	4	Mouse	4.8	pKd	=	4.8	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740898	109632	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	CHEMBL3236562	109632	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	10468601	193908	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202324	193908	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL555434	193908	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activityAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of 2-[3-(imidazol-4-yl)-propyl]-1-(4-iodo-benzyl)-isothiourea dihydrobromide-induced intrinsic activity	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	53233904	61181	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770965	61181	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	10446295	2092	29	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2092	29	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2092	29	None	11	2	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	4242975	18639	9	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128088	18639	9	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	9900313	19211	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129444	19211	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	9839217	72159	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199062	72159	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	11565330	96758	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	CHEMBL268525	96758	0	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	4906599	168086	10	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL436381	168086	10	None	-	0	Human	7.7	pKd	=	7.7	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	24996854	109634	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	CHEMBL3236564	109634	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	42624150	109647	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236577	109647	0	None	-	0	Human	8.6	pKd	=	8.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	24797956	109628	3	None	-	1	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	109628	3	None	-	1	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	24992735	109653	0	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	CHEMBL3236582	109653	0	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	10244243	140769	3	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL383384	140769	3	None	-	0	Human	7.6	pKd	=	7.6	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	46890592	6300	0	None	-	2	Human	7.6	pKd	=	7.6	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6300	0	None	-	2	Human	7.6	pKd	=	7.6	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44547127	115405	0	None	-	1	Human	8.5	pKd	=	8.5	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115405	0	None	-	1	Human	8.5	pKd	=	8.5	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	24992077	109641	0	None	-	0	Human	8.4	pKd	=	8.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236571	109641	0	None	-	0	Human	8.4	pKd	=	8.4	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	10934567	19047	1	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL129026	19047	1	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	11630326	72211	0	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199231	72211	0	None	-	0	Human	7.5	pKd	=	7.5	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	44547567	116604	2	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393533	116604	2	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	4060730	19168	51	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129199	19168	51	None	-	0	Human	7.4	pKd	=	7.4	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	53378711	109648	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236578	109648	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	24994634	2102	26	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2102	26	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2102	26	None	-	1	Mouse	8.3	pKd	=	8.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	54753437	67826	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915048	67826	0	None	-	0	Human	8.3	pKd	=	8.3	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	42624149	109638	1	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109638	1	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at mouse histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	46890590	6931	0	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6931	0	None	-	0	Mouse	7.3	pKd	=	7.3	Functional	Antagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challengeAntagonist activity at mouse histamine H4 receptor in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP release preincubated for 10 mins before forskolin challenge	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11572877	66730	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL187285	66730	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	9992865	113915	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL333220	113915	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	11601630	132283	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL370135	132283	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	46890546	6422	0	None	-	1	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6422	0	None	-	1	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890590	6931	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6931	0	None	-	0	Human	7.3	pKd	=	7.3	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	2063135	8278	9	None	-	1	Human	8.2	pKd	=	8.2	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1093180	8278	9	None	-	1	Human	8.2	pKd	=	8.2	Functional	Antagonist activity at recombinant human histamine H4 receptorAntagonist activity at recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	11066922	19210	1	None	-	0	Human	8.2	pKd	=	8.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129436	19210	1	None	-	0	Human	8.2	pKd	=	8.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	24994634	2102	26	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2102	26	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2102	26	None	-	1	Rat	7.2	pKd	=	7.2	Functional	Antagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at rat histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	10083188	140390	3	None	-	0	Human	7.2	pKd	=	7.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL382276	140390	3	None	-	0	Human	7.2	pKd	=	7.2	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	46890019	7216	0	None	-	1	Human	7.2	pKd	=	7.2	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7216	0	None	-	1	Human	7.2	pKd	=	7.2	Functional	Intrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assayIntrinsic activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-induced cAMP responsive element-beta-galactosidase activity after 6 hrs by colorimetric cAMP assay	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1278	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	10967174	19227	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19227	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Decrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptorDecrease in the forskolin stimulatedcAMP levels was determined using SK-N-MC cells stably transfected with the human H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	42624148	109644	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236574	109644	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysisAntagonist activity at human histamine H4 receptor expressed in SK-N-MC cells assessed as reduction of histamine-induced inhibition of cAMP-mediated beta-galactosidase reporter gene activity treated 10 mins prior to histamine challenge by microplate reader analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	54583875	61211	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770995	61211	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrsAntagonist activity at human histamine H4 receptor expressed in human SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP accumulation after 6 hrs	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227179	116619	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393548	116619	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	58227086	116628	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393557	116628	0	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonist activity at human recombinant histamine H4 receptor expressed in SK-N-MC cells assessed as inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2110	79	None	1	4	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	10967174	19227	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129540	19227	3	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	10988959	117592	4	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL340740	117592	4	None	-	0	Human	8.1	pKd	=	8.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	10923241	18957	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128958	18957	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	9878733	116331	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338051	116331	1	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	11623296	116509	0	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338972	116509	0	None	-	0	Human	7.1	pKd	=	7.1	Functional	Antagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activityAntagonistic activity at human histamine H4 receptor in SK-N-MC cells by inhibition of forskolin-stimulated cAMP-mediated reporter gene activity	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	16202258	67861	0	None	-	0	Human	10.5	pKi	=	10.5	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915536	67861	0	None	-	0	Human	10.5	pKi	=	10.5	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	24745506	67862	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915537	67862	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-	1	Human	8.8	pKi	=	8.8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	44187557	193817	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	CHEMBL1202332	193817	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	CHEMBL553423	193817	0	None	-	0	Human	8.8	pKi	=	8.8	Functional	Agonistic activity at histamine H4 receptorAgonistic activity at histamine H4 receptor	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm1013488
	135525668	67855	0	None	-	0	Human	8.0	pKi	=	8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915348	67855	0	None	-	0	Human	8.0	pKi	=	8	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	136042270	69557	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938979	69557	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24745507	67863	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915538	67863	0	None	-	0	Human	7.9	pKi	=	7.9	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	57401167	69511	0	None	-	0	Human	6.9	pKi	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938846	69511	0	None	-	0	Human	6.9	pKi	=	6.9	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400778	67853	0	None	-	0	Human	6.8	pKi	=	6.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915346	67853	0	None	-	0	Human	6.8	pKi	=	6.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	135525668	67855	0	None	-	0	Human	7.8	pKi	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915348	67855	0	None	-	0	Human	7.8	pKi	=	7.8	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57391926	69544	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938967	69544	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042269	69556	0	None	-	0	Human	7.7	pKi	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938978	69556	0	None	-	0	Human	7.7	pKi	=	7.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57402929	69509	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938844	69509	0	None	-	0	Human	6.7	pKi	=	6.7	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57395538	67854	0	None	-	0	Human	6.6	pKi	=	6.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915347	67854	0	None	-	0	Human	6.6	pKi	=	6.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042267	69554	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938976	69554	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400777	67852	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915345	67852	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	57400777	67852	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915345	67852	0	None	-	0	Human	7.6	pKi	=	7.6	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	49841846	69380	0	None	-	1	Human	8.5	pKi	=	8.5	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69380	0	None	-	1	Human	8.5	pKi	=	8.5	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	11688307	69545	0	None	-	0	Human	5.5	pKi	=	5.5	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938968	69545	0	None	-	0	Human	5.5	pKi	=	5.5	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	54754351	67860	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915535	67860	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	57400778	67853	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915346	67853	0	None	-	0	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	10446295	2092	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	1277	2092	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL185951	2092	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	10446295	2092	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	1277	2092	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL185951	2092	29	None	11	2	Human	7.4	pKi	=	7.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	49841719	69379	0	None	-	1	Human	8.4	pKi	=	8.4	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69379	0	None	-	1	Human	8.4	pKi	=	8.4	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	57391927	69546	0	None	-	0	Human	5.4	pKi	=	5.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938969	69546	0	None	-	0	Human	5.4	pKi	=	5.4	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1278	2110	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2110	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2110	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2110	79	None	1	4	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	49841845	69381	0	None	-	1	Human	8.3	pKi	=	8.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69381	0	None	-	1	Human	8.3	pKi	=	8.3	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	57395538	67854	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915347	67854	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	136042265	69552	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938974	69552	0	None	-	0	Human	7.3	pKi	=	7.3	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57395620	67859	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915534	67859	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	57395620	67859	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915534	67859	0	None	-	0	Human	6.2	pKi	=	6.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	1278	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1278	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1279	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	4908365	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL129198	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor by functional assayAntagonist activity at human histamine H4 receptor by functional assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1278	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2110	79	None	1	4	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1267	3732	43	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3732	43	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3732	43	None	-17	5	Human	7.2	pKi	=	7.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	56835171	69382	0	None	-	1	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69382	0	None	-	1	Human	8.2	pKi	=	8.2	Functional	Antagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS bindingAntagonist activity at human H4 receptor expressed in HEK293T cells assessed as inhibition of histamine-induced [35S]GTPgammaS binding	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	57390145	69548	0	None	-	0	Human	5.1	pKi	=	5.1	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938970	69548	0	None	-	0	Human	5.1	pKi	=	5.1	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	136042268	69555	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938977	69555	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136042266	69553	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938975	69553	0	None	-	0	Human	8.0	pKi	=	8.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	54754351	67860	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915535	67860	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assayAntagonist activity at full length human H4R expressed in HEK293 cells assessed as reversal of forskolin-induced cAMP production by CRE-beta-lactamase reporter gene assay	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	56950378	69510	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938845	69510	0	None	-	0	Human	7.0	pKi	=	7.0	Functional	Antagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assayAntagonist activity at human histamine H4 receptor expressed in HEK293 cells assessed as rev inhibition of forskolin-stimulated cAMP accumulation by CRE-betalactamase reporter gene assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	135	2486	38	None	-2	31	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1796	2486	38	None	-2	31	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	4184	2486	38	None	-2	31	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	CHEMBL6437	2486	38	None	-2	31	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	DB06148	2486	38	None	-2	31	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1225	1438	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	3958	1438	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	667477	1438	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	CHEMBL860	1438	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	DB01142	1438	24	None	-3	11	Human	8.2	pEC50	=	8.2	Functional	NoneNone	Drug Central	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	5344	172891	98	None	60	5	Human	8.3	pIC50	=	8.3	Functional	NoneNone	Drug Central	267	3	2	5	1.7	Cc1noc(NS(=O)(=O)c2ccc(N)cc2)c1C	None
	CHEMBL453	172891	98	None	60	5	Human	8.3	pIC50	=	8.3	Functional	NoneNone	Drug Central	267	3	2	5	1.7	Cc1noc(NS(=O)(=O)c2ccc(N)cc2)c1C	None
	1272	816	0	None	-	1	Human	8.1	pIC50	None	8.1	Functional	UnclassifiedUnclassified	Guide to Pharmacology		None	None	None		None	15265943

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Ligands					Receptor			Assay information							Chemical information
Sel. page	Common name	GPCRdb ID	#Vendors	Reference ligand	Fold selectivity (Affinity)	# tested GPCRs (Affinity)	Species	p-value (-log)	Type	Activity Relation	Activity Value	Assay Type	Assay Description	Source	Mol weight	Rot Bonds	H don	H acc	LogP	Smiles	DOI
	51354301	58569	0	None	-10	2	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	CHEMBL1688969	58569	0	None	-10	2	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/acs.jmedchem.8b00435
	70692530	75324	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL2047474	75324	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1021/acs.jmedchem.8b00435
	90667434	108988	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	2	3	1.9	CC(CC(=O)/N=C(\N)NCCCn1ccnc1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL3220892	108988	0	None	-	1	Human	6.0	pEC50	=	6	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	2	3	1.9	CC(CC(=O)/N=C(\N)NCCCn1ccnc1)c1ccccc1	10.1039/C3MD00245D
	44367568	45469	5	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@@H]2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153051	45469	5	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@@H]2CCNC2)c[nH]1	10.1021/jm030905y
	9877454	203891	1	None	-4	2	Human	5.0	pEC50	=	5.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL81644	203891	1	None	-4	2	Human	5.0	pEC50	=	5.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	155512725	175657	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175657	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175657	0	None	-	2	Mouse	6.0	pEC50	=	6.0	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	1267	3732	43	None	-33	28	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	3035905	3732	43	None	-33	28	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	CHEMBL260374	3732	43	None	-33	28	Human	7.0	pEC50	=	7.0	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm9000693
	9793791	45744	3	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	137	2	2	2	0.5	NCC1CC1c1c[nH]cn1	10.1021/jm030905y
	CHEMBL153284	45744	3	None	-	1	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	137	2	2	2	0.5	NCC1CC1c1c[nH]cn1	10.1021/jm030905y
	155524115	175963	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175963	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175963	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	1204	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1247	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1375	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	774	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	DB05381	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm800841w
	1236	2028	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	1237	2028	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	156615	2028	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	CHEMBL268229	2028	23	None	-204	7	Human	6.0	pEC50	=	6.0	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm030905y
	46890592	6300	0	None	-	1	Mouse	5.9	pEC50	=	5.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6300	0	None	-	1	Mouse	5.9	pEC50	=	5.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46890166	7220	0	None	-4786	2	Mouse	7.9	pEC50	=	7.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7220	0	None	-4786	2	Mouse	7.9	pEC50	=	7.9	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1204	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1247	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1375	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	774	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	DB05381	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm9000693
	1204	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1247	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1375	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	774	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	DB05381	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C3MD00245D
	1278	2110	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	1279	2110	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	4908365	2110	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitmentAgonist activity at histamine H4 receptor (unknown origin) assessed as increase in beta-arrestin recruitment	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.8b00435
	155524115	175963	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175963	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175963	0	None	1	3	Mouse	7.9	pEC50	=	7.9	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	11344458	203477	2	None	-14	2	Human	5.9	pEC50	=	5.9	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78497	203477	2	None	-14	2	Human	5.9	pEC50	=	5.9	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	1204	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1893	114	None	-3	25	Human	7.9	pEC50	=	7.9	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	24797956	109628	3	None	-	1	Human	5.9	pEC50	=	5.9	Binding	Agonist activity at human H4 receptorAgonist activity at human H4 receptor	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	109628	3	None	-	1	Human	5.9	pEC50	=	5.9	Binding	Agonist activity at human H4 receptorAgonist activity at human H4 receptor	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	58227373	175924	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175924	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175924	0	None	-	2	Mouse	6.9	pEC50	=	6.9	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	175894	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175894	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175894	0	None	38	2	Human	6.9	pEC50	=	6.9	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	46887115	8411	0	None	-	0	Human	5.9	pEC50	=	5.9	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)C[C@H](C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1094144	8411	0	None	-	0	Human	5.9	pEC50	=	5.9	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)C[C@H](C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	58227373	175924	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175924	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175924	0	None	1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155517880	176083	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	-	2	Rat	6.8	pEC50	=	6.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	25149335	183633	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm9000693
	CHEMBL483406	183633	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm9000693
	25149335	183633	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL483406	183633	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1039/C3MD00245D
	25149335	183633	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm800841w
	CHEMBL483406	183633	0	None	-	1	Human	7.8	pEC50	=	7.8	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/jm800841w
	45267230	194798	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202318	194798	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL563342	194798	0	None	-1	2	Human	7.8	pEC50	=	7.8	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	42625223	179335	0	None	-	1	Human	6.8	pEC50	=	6.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	395	8	4	3	1.4	CN(CCC(=O)/N=C(\N)NCCCc1c[nH]cn1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm9000693
	CHEMBL474617	179335	0	None	-	1	Human	6.8	pEC50	=	6.8	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	395	8	4	3	1.4	CN(CCC(=O)/N=C(\N)NCCCc1c[nH]cn1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm9000693
	11240759	104721	5	None	-190	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL311473	104721	5	None	-190	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	9812843	203523	1	None	-4	2	Human	4.8	pEC50	=	4.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78838	203523	1	None	-4	2	Human	4.8	pEC50	=	4.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	11469514	103678	2	None	-64	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL309654	103678	2	None	-64	2	Human	5.8	pEC50	=	5.8	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	155524115	175963	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175963	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175963	0	None	-	3	Rat	7.8	pEC50	=	7.8	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	127052293	139695	1	None	-	4	Mouse	6.8	pEC50	=	6.8	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139695	1	None	-	4	Mouse	6.8	pEC50	=	6.8	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	155513612	175736	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	1	4	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42626109	179178	0	None	-	1	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	324	6	4	2	1.5	N/C(=N\C(=O)Cc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL474447	179178	0	None	-	1	Human	7.7	pEC50	=	7.7	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	324	6	4	2	1.5	N/C(=N\C(=O)Cc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	155512725	175657	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175657	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175657	0	None	-	2	Rat	6.7	pEC50	=	6.7	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	42625386	177768	0	None	-	2	Human	8.6	pEC50	=	8.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	6	3	2	0.6	CCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL465170	177768	0	None	-	2	Human	8.6	pEC50	=	8.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	6	3	2	0.6	CCCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	46830167	8658	0	None	-	0	Human	8.6	pEC50	=	8.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096429	8658	0	None	-	0	Human	8.6	pEC50	=	8.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	11171320	45654	5	None	-	1	Human	5.7	pEC50	=	5.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	179	3	2	2	1.3	c1nc(CCC2CCCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153207	45654	5	None	-	1	Human	5.7	pEC50	=	5.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	179	3	2	2	1.3	c1nc(CCC2CCCNC2)c[nH]1	10.1021/jm030905y
	170389	205243	31	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	CHEMBL4634086	205243	31	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	CHEMBL91353	205243	31	None	1	3	Human	6.7	pEC50	=	6.7	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/jm030905y
	46887041	8657	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096428	8657	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	313	7	4	3	2.2	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)c1ccccc1	10.1016/j.bmcl.2010.03.082
	155517880	176083	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	-	2	Mouse	6.6	pEC50	=	6.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	1204	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-15	25	Mouse	5.6	pEC50	=	5.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	127052293	139695	1	None	34	4	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139695	1	None	34	4	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11301441	95694	6	None	-	1	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL260549	95694	6	None	-	1	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/acs.jmedchem.8b00435
	1278	2110	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	1279	2110	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	4908365	2110	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	CHEMBL129198	2110	79	None	-3	8	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assayAgonist activity at human H4R expressed in human U2OS cells assessed as recruitment of beta-arrestin after 2 hrs by PathHunter beta-galactosidase enzyme fragment complementation assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401677g
	58227401	175867	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175867	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175867	0	None	-	2	Mouse	7.6	pEC50	=	7.6	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-2	4	Rat	7.6	pEC50	=	7.6	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42625222	179180	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	367	7	5	3	0.7	N/C(=N\C(=O)CNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL474449	179180	0	None	-	0	Human	7.6	pEC50	=	7.6	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	367	7	5	3	0.7	N/C(=N\C(=O)CNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	1267	3732	43	None	-21	28	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-21	28	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-21	28	Mouse	7.6	pEC50	=	7.6	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	90667228	108968	0	None	-	1	Human	5.5	pEC50	=	5.5	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1ccccc1	10.1039/C3MD00245D
	CHEMBL3220629	108968	0	None	-	1	Human	5.5	pEC50	=	5.5	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	313	7	3	2	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1ccccc1	10.1039/C3MD00245D
	58227401	175867	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175867	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175867	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	42625303	180174	0	None	-	2	Human	8.5	pEC50	=	8.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL475621	180174	0	None	-	2	Human	8.5	pEC50	=	8.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	223	5	3	2	0.2	CCC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	42625304	178512	0	None	-	2	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	5	3	2	0.4	CC(C)C(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL471724	178512	0	None	-	2	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	237	5	3	2	0.4	CC(C)C(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	11286909	203563	2	None	-1	2	Human	5.5	pEC50	=	5.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL79126	203563	2	None	-1	2	Human	5.5	pEC50	=	5.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	9964223	203964	11	None	-36	2	Human	4.5	pEC50	=	4.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL82244	203964	11	None	-36	2	Human	4.5	pEC50	=	4.5	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	42626111	189092	0	None	-	0	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	381	8	5	3	1.1	N/C(=N\C(=O)CCNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL515428	189092	0	None	-	0	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	381	8	5	3	1.1	N/C(=N\C(=O)CCNC(=O)c1cc2ccccc2[nH]1)NCCCc1c[nH]cn1	10.1021/jm9000693
	1204	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-3	25	Human	7.5	pEC50	=	7.5	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	58227373	175924	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175924	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175924	0	None	-	2	Rat	7.5	pEC50	=	7.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	155552061	175894	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175894	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175894	0	None	-	2	Rat	6.5	pEC50	=	6.5	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	58227163	175879	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175879	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175879	0	None	-	2	Mouse	6.5	pEC50	=	6.5	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	127050776	139704	0	None	-	2	Mouse	5.4	pEC50	=	5.4	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	139704	0	None	-	2	Mouse	5.4	pEC50	=	5.4	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1893	114	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-12	25	Rat	5.4	pEC50	=	5.4	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	45269820	193669	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL1202326	193669	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	CHEMBL551790	193669	0	None	-1	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1021/acs.jmedchem.8b00435
	155512725	175657	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175657	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175657	0	None	154	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	16078981	43808	12	None	-	1	Human	5.4	pEC50	=	5.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	c1nc(CC2CCCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL151467	43808	12	None	-	1	Human	5.4	pEC50	=	5.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	c1nc(CC2CCCNC2)c[nH]1	10.1021/jm030905y
	155524115	175963	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175963	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175963	0	None	-2	3	Human	8.4	pEC50	=	8.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	46887043	8660	0	None	-	0	Human	8.3	pEC50	=	8.3	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096431	8660	0	None	-	0	Human	8.3	pEC50	=	8.3	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	42625384	169195	0	None	-	2	Human	8.3	pEC50	=	8.3	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	209	4	3	2	-0.2	CC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL443896	169195	0	None	-	2	Human	8.3	pEC50	=	8.3	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	209	4	3	2	-0.2	CC(=O)/N=C(\N)NCCCc1c[nH]cn1	10.1021/jm9000693
	44367567	168624	3	None	-	1	Human	6.4	pEC50	=	6.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@H]2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL440730	168624	3	None	-	1	Human	6.4	pEC50	=	6.4	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(C[C@H]2CCNC2)c[nH]1	10.1021/jm030905y
	58227163	175879	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175879	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175879	0	None	40	2	Human	7.4	pEC50	=	7.4	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assayAgonist activity at mouse recombinant H4R expressed in HEK293T cells by beta-arrestin recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	156013896	177655	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-22	4	Mouse	7.3	pEC50	=	7.3	Binding	Antagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assayAntagonist activity at mouse H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment preincubated for 15 mins followed by histamine addition by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	127049855	139685	0	None	-1	4	Human	6.3	pEC50	=	6.3	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	139685	0	None	-1	4	Human	6.3	pEC50	=	6.3	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11957573	1971	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	1250	1971	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	3692	1971	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	CHEMBL19439	1971	29	None	-40	6	Human	7.3	pEC50	=	7.3	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm020415q
	155513612	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-1	4	Mouse	7.3	pEC50	=	7.3	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	127051301	139657	0	None	-	2	Mouse	6.3	pEC50	=	6.3	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139657	0	None	-	2	Mouse	6.3	pEC50	=	6.3	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1204	1893	114	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1247	1893	114	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1375	1893	114	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	774	1893	114	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90	1893	114	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	DB05381	1893	114	None	-3	25	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	46235428	6511	0	None	-	1	Mouse	6.3	pEC50	=	6.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6511	0	None	-	1	Mouse	6.3	pEC50	=	6.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1251	1975	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	3035842	1975	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	CHEMBL18661	1975	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	1251	1975	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	3035842	1975	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	CHEMBL18661	1975	34	None	-100	5	Human	7.3	pEC50	=	7.3	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm030905y
	11206058	103923	9	None	-10	2	Human	4.2	pEC50	=	4.2	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL310360	103923	9	None	-10	2	Human	4.2	pEC50	=	4.2	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	127049855	139685	0	None	-	4	Mouse	6.2	pEC50	=	6.2	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	139685	0	None	-	4	Mouse	6.2	pEC50	=	6.2	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25149708	187842	0	None	-	0	Human	8.2	pEC50	=	8.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	319	7	4	4	2.2	CC(CC(=O)NC(=N)NCCCc1c[nH]cn1)c1cccs1	10.1021/jm800841w
	CHEMBL502339	187842	0	None	-	0	Human	8.2	pEC50	=	8.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	319	7	4	4	2.2	CC(CC(=O)NC(=N)NCCCc1c[nH]cn1)c1cccs1	10.1021/jm800841w
	58227401	175867	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175867	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175867	0	None	7	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	46887079	8409	0	None	-	0	Human	5.2	pEC50	=	5.2	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)CC(C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1094142	8409	0	None	-	0	Human	5.2	pEC50	=	5.2	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	365	7	4	5	3.2	Cc1nc(N)sc1CCCNC(=N)NC(=O)CC(C)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	127051301	139657	0	None	5	2	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139657	0	None	5	2	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at human SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130914	186661	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	CHEMBL492677	186661	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assayAgonist activity at histamine H4 receptor (unknown origin) expressed in human U2OS cells increase in beta-arrestin2 recruitment after 2 hrs by luminescence assay	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1021/acs.jmedchem.8b00435
	156015638	177687	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177687	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177687	0	None	-58	4	Human	7.2	pEC50	=	7.2	Binding	Agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assayAgonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as induction of beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	58227163	175879	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175879	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175879	0	None	-	2	Rat	6.2	pEC50	=	6.2	Binding	Agonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assayAgonist activity at rat H4R expressed in HEK293T-beta-arr2-rH4R cells by beta-arrestin2 recruitment assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25149613	178611	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	382	8	4	5	2.7	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1nccs1	10.1021/jm800841w
	CHEMBL472441	178611	0	None	-	1	Human	7.2	pEC50	=	7.2	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	382	8	4	5	2.7	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1nccs1	10.1021/jm800841w
	136773074	156811	0	None	-	1	Human	5.2	pEC50	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4077914	156811	0	None	-	1	Human	5.2	pEC50	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	42625221	188987	0	None	-	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	338	7	4	2	1.9	N/C(=N\C(=O)CCc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL514641	188987	0	None	-	2	Human	8.2	pEC50	=	8.2	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	338	7	4	2	1.9	N/C(=N\C(=O)CCc1c[nH]c2ccccc12)NCCCc1c[nH]cn1	10.1021/jm9000693
	24798144	109623	2	None	-19	3	Mouse	8.1	pEC50	=	8.1	Binding	Partial agonist activity at mouse histamine H4 receptorPartial agonist activity at mouse histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109623	2	None	-19	3	Mouse	8.1	pEC50	=	8.1	Binding	Partial agonist activity at mouse histamine H4 receptorPartial agonist activity at mouse histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	10374457	28667	2	None	-	2	Human	5.2	pEC50	=	5.2	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	123	1	2	2	0.2	N[C@H]1C[C@@H]1c1c[nH]cn1	10.1021/jm030905y
	CHEMBL13795	28667	2	None	-	2	Human	5.2	pEC50	=	5.2	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	123	1	2	2	0.2	N[C@H]1C[C@@H]1c1c[nH]cn1	10.1021/jm030905y
	11819221	45488	3	None	-	1	Human	6.1	pEC50	=	6.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(CC2CCNC2)c[nH]1	10.1021/jm030905y
	CHEMBL153061	45488	3	None	-	1	Human	6.1	pEC50	=	6.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	151	2	2	2	0.6	c1nc(CC2CCNC2)c[nH]1	10.1021/jm030905y
	1204	1893	114	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	1247	1893	114	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	1375	1893	114	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	774	1893	114	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	CHEMBL90	1893	114	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	DB05381	1893	114	None	-3	25	Human	7.1	pEC50	=	7.1	Binding	Inhibition of human Histamine H4 receptorInhibition of human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm020415q
	90667229	108969	0	None	-	0	Human	6.1	pEC50	=	6.1	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	319	7	3	3	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1cccs1	10.1039/C3MD00245D
	CHEMBL3220630	108969	0	None	-	0	Human	6.1	pEC50	=	6.1	Binding	Agonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assayAgonist activity at human recombinant histamine H4 receptor-RGS19 fusion protein expressed in Sf9 cells coexpressing Galphai2,Gbeta1gamma2 preincubated for 2 mins before [gamma32P]GTP addition measured after 20 mins by steady-state GTPase activity assay	ChEMBL	319	7	3	3	2.0	CC(CC(=O)/N=C(\N)NCCCc1ncc[nH]1)c1cccs1	10.1039/C3MD00245D
	11546127	95027	2	None	-	1	Human	5.1	pEC50	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL25725	95027	2	None	-	1	Human	5.1	pEC50	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	182527	110066	7	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	167	4	4	2	-0.2	N=C(N)NCCCc1c[nH]cn1	10.1021/jm9000693
	CHEMBL325327	110066	7	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Agonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation countingAgonist activity at human H4 receptor expressed in insect Sf9 cells co-expressing RGS19 fusion protein and Gialpha2, Gbeta1gamma2 assessed as gamma[32P]GTP binding by liquid scintillation counting	ChEMBL	167	4	4	2	-0.2	N=C(N)NCCCc1c[nH]cn1	10.1021/jm9000693
	46887042	8659	0	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	CHEMBL1096430	8659	0	None	-	0	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	319	7	4	3	2.6	C[C@H](CC(=O)NC(=N)NCCCc1c[nH]cn1)C1CCCCC1	10.1016/j.bmcl.2010.03.082
	25149521	178464	0	None	-	1	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1021/jm800841w
	CHEMBL471413	178464	0	None	-	1	Human	8.1	pEC50	=	8.1	Binding	Activity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assayActivity at human recombinant histamine H4 receptor-RGS4 fusion protein expressed in Sf9 cells coexpressing Galphai2 and G-beta-1-gamma-2 by steady-state GTPase activity assay	ChEMBL	375	8	4	3	3.2	N=C(NCCCc1c[nH]cn1)NC(=O)CC(c1ccccc1)c1ccccc1	10.1021/jm800841w
	155552061	175894	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175894	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175894	0	None	-	2	Mouse	6.1	pEC50	=	6.1	Binding	Agonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assayAgonist activity at mouse H4R expressed in HEK293T-beta-arr2-mH4R cells by beta-arrestin2 recruitment assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	155517880	176083	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	16	2	Human	7.1	pEC50	=	7.1	Binding	Agonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assayAgonist activity at human H4R expressed in HEK293T-beta-arr2-hH4R cells by beta-arrestin2 recruitment assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	1249	1970	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	13499360	1970	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	CHEMBL90019	1970	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	1249	1970	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	13499360	1970	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90019	1970	14	None	-3	3	Human	7.1	pEC50	=	7.1	Binding	Inhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibitory activity determined by the inhibition of cAMP- stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm030905y
	1204	1893	114	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	1247	1893	114	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	1375	1893	114	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	774	1893	114	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	CHEMBL90	1893	114	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	DB05381	1893	114	None	-3	25	Human	8.0	pEC50	=	8.0	Binding	Activity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assayActivity at human recombinant GAIP-fused histamine H4 receptor expressed in Sf9 cells coexpressing Galphai, Gbeta1gamma2 by steady-state GTPase activity assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.03.082
	4024	1976	14	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	9989505	1976	14	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	CHEMBL82298	1976	14	None	-301	2	Human	6.0	pEC50	=	6.0	Binding	Inhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptorInhibition of the cAMP-stimulated beta-galactosidase transcription in SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	1204	1893	114	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1247	1893	114	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	1375	1893	114	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	774	1893	114	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90	1893	114	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	DB05381	1893	114	None	-15	25	Mouse	7.0	pEC50	=	7.0	Binding	Agonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assayAgonist activity at mouse SF-His6-tagged histamine H4 receptor expressed in HEK293 cells assessed as inhibition of forskolin-stimulated response incubated for 5 hrs by luciferase reporter gene assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	16718567	8063	0	None	-	1	Human	9.0	pIC50	=	9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091874	8063	0	None	-	1	Human	9.0	pIC50	=	9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885297	8315	0	None	-	0	Human	6.0	pIC50	=	6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	318	1	1	6	2.5	CN1CCN(c2nc(O)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093438	8315	0	None	-	0	Human	6.0	pIC50	=	6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	318	1	1	6	2.5	CN1CCN(c2nc(O)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	17747460	60985	8	None	-	4	Human	5.0	pIC50	=	5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	640	14	0	6	7.8	O=c1c2ccccc2c(Cc2ccc(Cl)cc2)nn1C[C@H]1CCCN1CCCCc1ccc(OCCCN2CCCCCC2)cc1	10.1021/jm1013874
	CHEMBL1767164	60985	8	None	-	4	Human	5.0	pIC50	=	5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	640	14	0	6	7.8	O=c1c2ccccc2c(Cc2ccc(Cl)cc2)nn1C[C@H]1CCCN1CCCCc1ccc(OCCCN2CCCCCC2)cc1	10.1021/jm1013874
	46884945	8146	0	None	-	0	Human	5.0	pIC50	=	5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	330	1	0	5	3.6	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CC(C)N1C	10.1016/j.bmcl.2010.02.097
	CHEMBL1092331	8146	0	None	-	0	Human	5.0	pIC50	=	5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	330	1	0	5	3.6	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CC(C)N1C	10.1016/j.bmcl.2010.02.097
	71547931	182519	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3c3ncnn23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4794724	182519	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3c3ncnn23)C1	10.1021/acs.jmedchem.7b01855
	11335478	160406	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL4115693	160406	0	None	-	0	Human	6.0	pIC50	=	6.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	1224	1399	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	3100	1399	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	8980	1399	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	916	1399	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	CHEMBL657	1399	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	DB01075	1399	79	None	-194	13	Human	5.0	pIC50	=	5.0	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	11709443	8147	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092332	8147	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	314	1	0	5	2.9	CN1C[C@@H]2C[C@H]1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71548405	181567	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	0	7	1.1	CN(C)C1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782441	181567	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	0	7	1.1	CN(C)C1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	46884998	7880	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	1	1	5	2.6	NC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090532	7880	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	1	1	5	2.6	NC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	25112105	8064	0	None	-	1	Human	7.9	pIC50	=	7.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091875	8064	0	None	-	1	Human	7.9	pIC50	=	7.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	71547681	182353	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.7	Clc1cnc2nc(N3CCNCC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4792733	182353	0	None	-	0	Human	6.9	pIC50	=	6.9	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.7	Clc1cnc2nc(N3CCNCC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	46885128	7873	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.2	CNC1(C)CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090519	7873	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.2	CNC1(C)CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885129	7874	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	1	5	3.2	Clc1ccc2oc3c(N4CC5CCCNC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090520	7874	0	None	-	0	Human	5.9	pIC50	=	5.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	1	5	3.2	Clc1ccc2oc3c(N4CC5CCCNC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	46885241	8176	0	None	-	0	Human	4.9	pIC50	=	4.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	2	1	5	3.8	CN[C@@H]1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092543	8176	0	None	-	0	Human	4.9	pIC50	=	4.9	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	2	1	5	3.8	CN[C@@H]1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	156013896	177655	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	7.8	pIC50	=	7.8	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	16656869	8005	0	None	-	0	Human	5.8	pIC50	=	5.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.1	CNC1CCN(c2nc(C)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091559	8005	0	None	-	0	Human	5.8	pIC50	=	5.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	1	5	3.1	CNC1CCN(c2nc(C)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	124087	1357	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	7157	1357	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	814	1357	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	CHEMBL1172	1357	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	DB00967	1357	106	None	-5888	15	Human	4.8	pIC50	=	4.8	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	11566111	8247	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1CC2CCC1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	CHEMBL1092933	8247	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1CC2CCC1CN2c1ncnc2c1oc1ccc(Cl)cc12	10.1016/j.bmcl.2010.02.097
	71547932	182175	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3c3ncnn23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4790239	182175	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3c3ncnn23)CC1	10.1021/acs.jmedchem.7b01855
	13680692	182842	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	313	2	0	8	0.9	CN1CCN(c2nc3ccc([N+](=O)[O-])cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4798729	182842	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	313	2	0	8	0.9	CN1CCN(c2nc3ccc([N+](=O)[O-])cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885239	8095	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092064	8095	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2010.02.097
	71547762	182127	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.7	CN1CCN(c2nc3nc(Cl)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789645	182127	0	None	-	0	Human	6.8	pIC50	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.7	CN1CCN(c2nc3nc(Cl)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	2063135	8278	9	None	-	1	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093180	8278	9	None	-	1	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	11695388	8145	0	None	-	0	Human	5.7	pIC50	=	5.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.2	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CCN1C	10.1016/j.bmcl.2010.02.097
	CHEMBL1092330	8145	0	None	-	0	Human	5.7	pIC50	=	5.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.2	CC1CN(c2ncnc3c2oc2ccc(Cl)cc23)CCN1C	10.1016/j.bmcl.2010.02.097
	9904236	13947	16	None	-	1	Human	5.7	pIC50	=	5.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL1197564	13947	16	None	-	1	Human	5.7	pIC50	=	5.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46884997	7624	0	None	-	0	Human	4.7	pIC50	=	4.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	0	5	3.2	CN(C)C1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088888	7624	0	None	-	0	Human	4.7	pIC50	=	4.7	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	2	0	5	3.2	CN(C)C1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1530	2142	44	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	3827	2142	44	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	7206	2142	44	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	CHEMBL534	2142	44	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	DB00920	2142	44	None	-21379	21	Human	4.7	pIC50	=	4.7	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	20889070	181280	5	None	-	0	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	302	1	0	6	1.7	CN1CCN(c2nc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778864	181280	5	None	-	0	Human	7.7	pIC50	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	302	1	0	6	1.7	CN1CCN(c2nc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	820836	102455	10	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	268	1	0	6	1.0	CN1CCN(c2nc3ccccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL306316	102455	10	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	268	1	0	6	1.0	CN1CCN(c2nc3ccccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	121237834	146814	0	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL3929465	146814	0	None	-	0	Human	5.6	pIC50	=	5.6	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	ChEMBL	297	0	2	3	3.5	O[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46884996	7900	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	Clc1ccc2oc3c(N4CCN5CCCC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090655	7900	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	Clc1ccc2oc3c(N4CCN5CCCC5C4)ncnc3c2c1	10.1016/j.bmcl.2010.02.097
	71548223	181268	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	401	3	0	8	2.0	CN1CCN(c2nc3nc(OCC(F)(F)F)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778712	181268	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	401	3	0	8	2.0	CN1CCN(c2nc3nc(OCC(F)(F)F)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71547685	182398	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	294	1	0	8	0.3	CN1CCN(c2nc3ncc(C#N)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4793425	182398	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	294	1	0	8	0.3	CN1CCN(c2nc3ncc(C#N)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548412	1870	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1870	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1870	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	71547764	181761	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	328	1	0	8	0.9	CN1CCN(c2nc3nc(C#N)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4784683	181761	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	328	1	0	8	0.9	CN1CCN(c2nc3nc(C#N)c(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548498	181648	0	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	348	1	0	8	0.6	CN1CCN(c2nc3ncc(Br)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4783487	181648	0	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	348	1	0	8	0.6	CN1CCN(c2nc3ncc(Br)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71548031	182316	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.4	CN1CCN(c2nc3ncc(C(F)(F)F)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4792352	182316	0	None	-	0	Human	6.6	pIC50	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	337	1	0	7	1.4	CN1CCN(c2nc3ncc(C(F)(F)F)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	16656871	7879	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090531	7879	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2110	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	1279	2110	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	4908365	2110	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	CHEMBL129198	2110	79	None	-3	8	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.7b01855
	71548412	1870	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	9879	1870	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	CHEMBL4790724	1870	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant mouse histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	10.1021/acs.jmedchem.7b01855
	71548319	182646	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	CN1CCCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4796300	182646	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	CN1CCCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885178	7617	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	336	2	1	5	3.2	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088859	7617	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	336	2	1	5	3.2	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2010.02.097
	71548224	181936	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	361	1	0	7	1.5	Cc1c(Br)cnc2nc(N3CCN(C)CC3)c3nncn3c12	10.1021/acs.jmedchem.7b01855
	CHEMBL4787243	181936	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	361	1	0	7	1.5	Cc1c(Br)cnc2nc(N3CCN(C)CC3)c3nncn3c12	10.1021/acs.jmedchem.7b01855
	71547599	181476	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3ncccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781409	181476	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3ncccc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	71547680	182235	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3cccnc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4791152	182235	0	None	-	0	Human	5.5	pIC50	=	5.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	269	1	0	7	0.4	CN1CCN(c2nc3cccnc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	11709905	8271	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	342	2	0	5	3.7	CN1CCN(c2nc(C3CC3)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093138	8271	0	None	-	0	Human	6.5	pIC50	=	6.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	342	2	0	5	3.7	CN1CCN(c2nc(C3CC3)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	16656872	7815	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1090190	7815	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	71548027	181258	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	395	1	0	7	1.0	CN1CCN(c2nc3ncc(I)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778591	181258	0	None	-	0	Human	7.5	pIC50	=	7.5	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	395	1	0	7	1.0	CN1CCN(c2nc3ncc(I)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	3934857	8004	23	None	-	0	Human	7.4	pIC50	=	7.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.1	Cc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091558	8004	23	None	-	0	Human	7.4	pIC50	=	7.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	316	1	0	5	3.1	Cc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	71547282	182481	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1C	10.1021/acs.jmedchem.7b01855
	CHEMBL4794336	182481	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	1	0	7	1.5	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1C	10.1021/acs.jmedchem.7b01855
	1278	2110	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2110	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2110	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2110	79	None	-4	8	Mouse	7.4	pIC50	=	7.4	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cellsInhibition of histamine H4 receptor-mediated chemotaxis in mouse bone marrow cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	89477685	182982	0	None	-	0	Human	5.4	pIC50	=	5.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	1	7	1.1	CN[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4800462	182982	0	None	-	0	Human	5.4	pIC50	=	5.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	2	1	7	1.1	CN[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	89477651	182856	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	315	1	0	7	1.2	CN1C[C@@H]2C[C@H]1CN2c1nc2ncc(Cl)cc2n2cnnc12	10.1021/acs.jmedchem.7b01855
	CHEMBL4798915	182856	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	315	1	0	7	1.2	CN1C[C@@H]2C[C@H]1CN2c1nc2ncc(Cl)cc2n2cnnc12	10.1021/acs.jmedchem.7b01855
	11638468	8248	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1C2CCC1CN(c1ncnc3c1oc1ccc(Cl)cc13)C2	10.1016/j.bmcl.2010.02.097
	CHEMBL1092934	8248	0	None	-	0	Human	6.4	pIC50	=	6.4	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	328	1	0	5	3.3	CN1C2CCC1CN(c1ncnc3c1oc1ccc(Cl)cc13)C2	10.1016/j.bmcl.2010.02.097
	71547281	182695	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	2	0	7	1.5	CN(C)[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4796842	182695	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	317	2	0	7	1.5	CN(C)[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	1267	3732	43	None	-33	28	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pIC50	=	6.3	Binding	Inverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assayInverse agonist activity at human H4R expressed in HEK293 cells co-expressing ELucC/ELucN-beta-arrestin2 assessed as inhibition of histamine-induced beta-arrestin recruitment by luciferase reporter gene assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	89477709	182102	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	1	7	0.9	Clc1cnc2nc(N3C[C@@H]4C[C@H]3CN4)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789385	182102	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	1	7	0.9	Clc1cnc2nc(N3C[C@@H]4C[C@H]3CN4)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547597	182539	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	283	1	0	7	0.7	Cc1cnc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4795091	182539	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	283	1	0	7	0.7	Cc1cnc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547848	182445	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	329	1	0	7	1.6	Clc1cnc2nc(N3CCN4CCCC4C3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4793974	182445	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	329	1	0	7	1.6	Clc1cnc2nc(N3CCN4CCCC4C3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71547765	181896	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	3	0	8	1.5	CCOc1nc2nc(N3CCN(C)CC3)c3nncn3c2cc1Cl	10.1021/acs.jmedchem.7b01855
	CHEMBL4786816	181896	0	None	-	0	Human	6.3	pIC50	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	3	0	8	1.5	CCOc1nc2nc(N3CCN(C)CC3)c3nncn3c2cc1Cl	10.1021/acs.jmedchem.7b01855
	71547682	181610	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	275	1	1	7	0.5	NC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782945	181610	0	None	-	0	Human	5.3	pIC50	=	5.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	275	1	1	7	0.5	NC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	89477439	181484	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	290	2	1	8	0.1	CNC1CN(c2nc3ncc(Cl)cc3n3nnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781484	181484	0	None	-	0	Human	7.3	pIC50	=	7.3	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	290	2	1	8	0.1	CNC1CN(c2nc3ncc(Cl)cc3n3nnnc23)C1	10.1021/acs.jmedchem.7b01855
	58475282	181060	0	None	-	0	Human	6.2	pIC50	=	6.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	282	1	0	6	1.3	Cc1ccc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	CHEMBL4776004	181060	0	None	-	0	Human	6.2	pIC50	=	6.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	282	1	0	6	1.3	Cc1ccc2nc(N3CCN(C)CC3)c3nncn3c2c1	10.1021/acs.jmedchem.7b01855
	71548411	181987	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	304	1	0	8	0.5	CN1CCN(c2nc3ncc(Cl)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4787983	181987	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	304	1	0	8	0.5	CN1CCN(c2nc3ncc(Cl)cc3n3nnnc23)CC1	10.1021/acs.jmedchem.7b01855
	53380276	182212	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	0	5	2.3	CN1CCN(c2cc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4790726	182212	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	301	1	0	5	2.3	CN1CCN(c2cc3ccc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	46885181	8238	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	293	2	1	6	2.0	CNC1CCN(c2ncnc3c2oc2ccc(C#N)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092880	8238	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	293	2	1	6	2.0	CNC1CCN(c2ncnc3c2oc2ccc(C#N)cc23)C1	10.1016/j.bmcl.2010.02.097
	89477539	182118	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.9	N[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4789543	182118	0	None	-	0	Human	5.2	pIC50	=	5.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	1	1	7	0.9	N[C@@H]1CCN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	71515465	181552	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	CHEMBL4782303	181552	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	289	2	1	7	0.7	CNC1CN(c2nc3ncc(Cl)cc3n3cnnc23)C1	10.1021/acs.jmedchem.7b01855
	71547683	181212	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	1	0	7	1.2	CN1CCN(c2nc3ncc(Br)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4778068	181212	0	None	-	0	Human	7.2	pIC50	=	7.2	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	347	1	0	7	1.2	CN1CCN(c2nc3ncc(Br)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	89477445	181458	0	None	-	0	Human	6.1	pIC50	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	1	7	1.1	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1	10.1021/acs.jmedchem.7b01855
	CHEMBL4781143	181458	0	None	-	0	Human	6.1	pIC50	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	1	7	1.1	C[C@H]1CN(c2nc3ncc(Cl)cc3n3cnnc23)CCN1	10.1021/acs.jmedchem.7b01855
	71547678	182748	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	CHEMBL4797517	182748	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in CHO-K1 cell membranes measured after 30 mins by microbeta scintillation counting method	ChEMBL	303	1	0	7	1.1	CN1CCN(c2nc3ncc(Cl)cc3n3cnnc23)CC1	10.1021/acs.jmedchem.7b01855
	24994634	2102	26	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2102	26	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2102	26	None	2	7	Mouse	8.1	pIC50	=	8.1	Binding	mBMMC chemotaxismBMMC chemotaxis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	11682208	8175	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1092542	8175	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	370	1	0	5	3.8	CN1CCN(c2nc(C(F)(F)F)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	46885177	8030	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	282	2	1	5	2.5	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091729	8030	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	282	2	1	5	2.5	CN[C@@H]1CCN(c2ncnc3c2oc2ccc(C)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2110	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2110	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2110	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2110	79	None	-3	8	Human	7.1	pIC50	=	7.1	Binding	Inhibition of histamine H4 receptor-mediated chemotaxis in human eosinophilsInhibition of histamine H4 receptor-mediated chemotaxis in human eosinophils	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	16656874	8007	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1091569	8007	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	288	2	1	5	2.4	CNC1CN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	46885298	8316	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	332	2	0	6	2.8	COc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093439	8316	0	None	-	0	Human	7.1	pIC50	=	7.1	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	332	2	0	6	2.8	COc1nc(N2CCN(C)CC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2010.02.097
	16656870	7625	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CNC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	CHEMBL1088889	7625	0	None	-	0	Human	7.0	pIC50	=	7.0	Binding	Displacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assayDisplacement of [3H]histamine dihydrochloride from human histamine H4 receptor after 2.5 hrs by scintillation proximity assay	ChEMBL	302	2	1	5	2.8	CNC1CCN(c2ncnc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2010.02.097
	1278	2110	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1279	2110	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	4908365	2110	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL129198	2110	79	None	-3	8	Human	8.0	pKd	=	8	Binding	pA2 value against histamine h4 receptorpA2 value against histamine h4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	71525681	89284	0	None	2	6	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89284	0	None	2	6	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846300	79841	3	None	-2	4	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79841	3	None	-2	4	Human	8.0	pKd	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	156013896	177655	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	942	89	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	942	89	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	942	89	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	942	89	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	942	89	None	-57	92	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	24994634	2102	26	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2102	26	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2102	26	None	-2	7	Human	7.9	pKd	=	7.9	Binding	Cell based cAMP Assays (pA2)Cell based cAMP Assays (pA2)	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	11846300	79841	3	None	-2	4	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79841	3	None	-2	4	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 10 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	71519674	89285	0	None	1	5	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89285	0	None	1	5	Human	7.9	pKd	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	1204	1893	114	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-12	25	Rat	6.9	pKd	=	6.9	Binding	Binding affinity to rat H4RBinding affinity to rat H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	135398737	942	89	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	942	89	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	942	89	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	942	89	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	942	89	None	-57	92	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	155513612	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156015638	177687	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177687	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177687	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1247	1893	114	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1375	1893	114	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	774	1893	114	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	CHEMBL90	1893	114	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	DB05381	1893	114	None	-3	25	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	119570	3098	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	2233	3098	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	953	3098	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	CHEMBL301265	3098	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	DB00413	3098	90	None	-301	40	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	10.1021/acs.jmedchem.1c00692
	168268716	192150	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5170276	192150	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221176	192150	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168268680	192154	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5172675	192154	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221210	192154	0	None	-2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	168268827	192167	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5179906	192167	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221314	192167	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	168268853	192173	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5180504	192173	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221353	192173	0	None	-83	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	168273471	192189	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173079	192189	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221433	192189	0	None	-50	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168273549	192191	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174342	192191	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221438	192191	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	168273640	192193	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5176229	192193	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221444	192193	0	None	-12	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	568	15	8	10	-0.1	N/C(=N\C(=O)NCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168273745	192196	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5178472	192196	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221451	192196	0	None	-11	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	4	4	1.5	N/C(=N\C(=O)NCc1ccccc1)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	168274129	192197	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5176780	192197	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221468	192197	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	370	12	5	6	1.0	NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168275750	192214	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5175558	192214	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221581	192214	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	332	6	3	4	1.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCc2ccccc2)s1	10.1021/acs.jmedchem.1c00692
	168276867	192223	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174278	192223	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221653	192223	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	168277996	192234	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173373	192234	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221736	192234	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	168278471	192236	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183205	192236	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221757	192236	0	None	-17	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	4	4	1.5	CCCCCNC(=O)/N=C(\N)NCCCc1cnc(N)s1	10.1021/acs.jmedchem.1c00692
	168278543	192239	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184846	192239	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221764	192239	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168280339	192253	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5188592	192253	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221885	192253	0	None	-3	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	8	3	5	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCn1cc(CN(C)C)nn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168281536	192269	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5189006	192269	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221966	192269	0	None	2	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	369	12	4	5	1.7	Cc1nnc(CCCN/C(N)=N/C(=O)NCCCCCCCCN)s1	10.1021/acs.jmedchem.1c00692
	168281746	192270	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184911	192270	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221974	192270	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	365	6	4	5	1.6	CC(NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1cccc(F)c1	10.1021/acs.jmedchem.1c00692
	168283097	192281	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	CHEMBL5186770	192281	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222043	192281	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	311	9	4	5	0.8	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)o1	10.1021/acs.jmedchem.1c00692
	168283321	192283	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183015	192283	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222057	192283	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	168283536	192288	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5187758	192288	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222074	192288	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	312	8	3	4	1.6	CCCCCNC(=O)/N=C(/N)NCCCc1nnc(C)s1	10.1021/acs.jmedchem.1c00692
	168284289	192294	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5194860	192294	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222123	192294	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168284480	192298	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5197548	192298	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222137	192298	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	330	6	4	4	1.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1021/acs.jmedchem.1c00692
	168286141	192312	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5192993	192312	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222231	192312	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	168286426	192314	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5198795	192314	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222241	192314	0	None	-109	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168290235	192356	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5201074	192356	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222491	192356	0	None	-1071	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	315	6	4	3	1.1	C[C@@H](NC(=O)/N=C(\N)NCCCc1ncn[nH]1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168290815	192361	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5200771	192361	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222529	192361	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	327	9	4	5	1.2	CCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168291233	192367	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5200106	192367	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222556	192367	0	None	5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	373	8	4	5	1.4	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)o2)cc1	10.1021/acs.jmedchem.1c00692
	168292954	192385	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5202592	192385	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222660	192385	0	None	-112	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	168294161	192394	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204739	192394	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222742	192394	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	168294445	192398	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208113	192398	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222768	192398	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	168294166	192402	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209081	192402	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222775	192402	0	None	-4	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	168294449	192406	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204599	192406	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222793	192406	0	None	-181	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	168294767	192407	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207281	192407	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222802	192407	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	313	8	4	5	0.9	CCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168294770	192408	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208327	192408	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222806	192408	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	168294776	192414	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209479	192414	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222831	192414	0	None	7	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	366	7	3	4	2.6	Cc1nnc(CCCN/C(N)=N/C(=O)NCC(C)C2CCCCC2)s1	10.1021/acs.jmedchem.1c00692
	168295528	192419	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5206565	192419	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222872	192419	0	None	-301	20	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	333	6	4	5	0.9	N/C(=N\C(=O)NCc1ccccc1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	168294178	192423	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5205197	192423	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222887	192423	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168295541	192428	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208845	192428	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222926	192428	0	None	-1071	6	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	347	6	4	5	1.4	C[C@@H](NC(=O)/N=C(\N)NCCCc1nnc(N)s1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	168295543	192429	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209021	192429	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222929	192429	0	None	-8	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	168295803	192433	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207206	192433	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222937	192433	0	None	-6	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168294182	192436	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208722	192436	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222960	192436	0	None	-5	4	Human	7.8	pKd	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	156013896	177655	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156015638	177687	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177687	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177687	0	None	-158	4	Mouse	6.8	pKd	=	6.8	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-1	4	Mouse	7.8	pKd	=	7.8	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177655	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	7.8	pKd	=	7.8	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	942	89	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	942	89	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	942	89	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	942	89	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	942	89	None	-57	92	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 300 nM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	71525679	89282	0	None	6	6	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89282	0	None	6	6	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	155513612	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-2	4	Rat	7.7	pKd	=	7.7	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	11846300	79841	3	None	-2	4	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79841	3	None	-2	4	Human	7.7	pKd	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 30 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	24745335	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	4	5	Human	7.6	pKd	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	1278	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	1279	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	4908365	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2013.02.091
	1223	938	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	2790	938	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	CHEMBL14690	938	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	1223	938	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	2790	938	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	CHEMBL14690	938	32	None	-60	11	Human	7.5	pKd	=	7.5	Binding	Binding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assayBinding affinity to NLuc-tagged human H4R expressed in HEK293T cell homogenates using NanoGlo as substrate preincubated for 2 hrs followed by substrate addition measured after 5 min by NanoBRET saturation binding assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.2c00125
	156013896	177655	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-22	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-1	4	Mouse	7.5	pKd	=	7.5	Binding	Binding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-2	4	Rat	7.5	pKd	=	7.5	Binding	Binding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177655	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	56835171	69382	0	None	1	9	Human	7.4	pKd	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL1935574	69382	0	None	1	9	Human	7.4	pKd	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	155513612	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	1	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as equilibrium dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177655	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	7.4	pKd	=	7.4	Binding	Binding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant human N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-15	25	Mouse	7.4	pKd	=	7.4	Binding	Binding affinity to mouse H4RBinding affinity to mouse H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	156013896	177655	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1247	1893	114	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1375	1893	114	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	774	1893	114	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL90	1893	114	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	DB05381	1893	114	None	-3	25	Human	8.3	pKd	=	8.3	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	156013896	177655	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-22	4	Mouse	7.3	pKd	=	7.3	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	155513612	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells assessed as kinetically derived dissociation constant by saturation binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	155513612	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	1	4	Human	7.2	pKd	=	7.2	Binding	Binding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding studyBinding affinity to human H4R expressed in HEK293T-SF-His6-CRE-Luc cells by kinetic binding study	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156013896	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156013896	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometryBinding affinity to recombinant mouse N- terminal NLuc-tagged H4R expressed in HEK293T cells by flow cytometry	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156013896	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-22	4	Mouse	7.2	pKd	=	7.2	Binding	Binding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	135398737	942	89	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	942	89	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	942	89	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	942	89	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	942	89	None	-57	92	Human	7.2	pKd	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uMDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells at 1 uM	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	11846300	79841	3	None	-2	4	Human	7.1	pKd	=	7.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79841	3	None	-2	4	Human	7.1	pKd	=	7.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nMDisplacement of [3H]Histamine from human histamine H4 receptor transfected in SK-N-MC cells at 100 nM	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	156015638	177687	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177687	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177687	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	156015638	177687	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177687	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177687	0	None	-58	4	Human	7.1	pKd	=	7.1	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	71525678	89281	0	None	5	6	Human	8.1	pKd	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89281	0	None	5	6	Human	8.1	pKd	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysisDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells by competitive kinetic analysis	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	156013896	177655	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	8.0	pKd	=	8.0	Binding	Binding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assayBinding affinity to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells assessed as dissociation constant measured after 30 mins in presence of thioperamide by furimazine substrate based BRET assay	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	54587788	61191	0	None	-	1	Human	10.4	pKi	=	10.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	4	3	5	1.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770975	61191	0	None	-	1	Human	10.4	pKi	=	10.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	4	3	5	1.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	23653543	67691	2	None	-	1	Human	10.0	pKi	=	10	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914541	67691	2	None	-	1	Human	10.0	pKi	=	10	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	54587787	61187	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770971	61187	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	5	3	5	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	46235428	6511	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083162	6511	0	None	-	1	Human	9.7	pKi	=	9.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	407	9	3	4	4.3	Cc1cc(OCCCNCCc2c[nH]cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	54586763	61184	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	3	2	5	1.9	CN1CCN(c2cc(N)nc(NCC(C)(C)C)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770968	61184	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	3	2	5	1.9	CN1CCN(c2cc(N)nc(NCC(C)(C)C)c2)CC1	10.1016/j.bmcl.2011.03.017
	24826169	67683	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914462	67683	0	None	-	1	Human	9.5	pKi	=	9.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	16718567	8063	0	None	-	1	Human	9.4	pKi	=	9.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1091874	8063	0	None	-	1	Human	9.4	pKi	=	9.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737950	67774	0	None	-	1	Human	9.2	pKi	=	9.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	361	2	2	6	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914781	67774	0	None	-	1	Human	9.2	pKi	=	9.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	361	2	2	6	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	58227148	116618	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393547	116618	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	57399746	67749	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914755	67749	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	54580885	61189	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	4	3	5	1.8	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770973	61189	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	4	3	5	1.8	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	24826766	67744	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914750	67744	0	None	-	1	Human	9.1	pKi	=	9.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CNC1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	59777338	109626	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236556	109626	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	1204	1893	114	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1247	1893	114	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1375	1893	114	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	774	1893	114	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	CHEMBL90	1893	114	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	DB05381	1893	114	None	-3	25	Human	9.0	pKi	=	9	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmc.2020.115924
	1223	938	32	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	938	32	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	938	32	None	-60	11	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	54581940	61208	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770992	61208	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(N)nc(NCC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	24797598	61218	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771001	61218	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(NC3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	11224088	135921	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1021/jm800876b
	CHEMBL373579	135921	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1021/jm800876b
	24798144	109623	2	None	19	3	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3236553	109623	2	None	19	3	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	58227342	116605	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393534	116605	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	11224088	135921	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	CHEMBL373579	135921	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	24825998	67754	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914760	67754	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)CC1	10.1016/j.bmcl.2011.08.014
	57396301	67776	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	2.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914783	67776	0	None	-	1	Human	9.0	pKi	=	9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	2.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(C(F)(F)F)cc23)C1	10.1016/j.bmcl.2011.08.014
	127050310	140291	0	None	-	1	Human	9.0	pKi	=	9	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	347	3	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(C(C)(F)F)cc23)C1	10.1039/C6MD00108D
	CHEMBL3819260	140291	0	None	-	1	Human	9.0	pKi	=	9	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	347	3	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(C(C)(F)F)cc23)C1	10.1039/C6MD00108D
	46222048	8881	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1098230	8881	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1016/j.bmc.2010.04.052
	24994542	109624	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236554	109624	0	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	24948752	67690	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.4	Nc1nc(N2CCCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914540	67690	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.4	Nc1nc(N2CCCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	24825727	67692	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914542	67692	2	None	-	1	Human	9.0	pKi	=	9.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	46222048	8881	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Inhibition of human recombinant histamine H4 receptor expressed in CHO cellsInhibition of human recombinant histamine H4 receptor expressed in CHO cells	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1021/jm901890s
	CHEMBL1098230	8881	0	None	-4	13	Human	9.0	pKi	=	9.0	Binding	Inhibition of human recombinant histamine H4 receptor expressed in CHO cellsInhibition of human recombinant histamine H4 receptor expressed in CHO cells	ChEMBL	260	4	1	2	4.0	CC(C)(C)c1ccc(SCCc2c[nH]cn2)cc1	10.1021/jm901890s
	155524115	175963	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175963	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175963	0	None	-2	3	Human	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	24798144	109623	2	None	19	3	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109623	2	None	19	3	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	155524115	175963	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175963	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175963	0	None	1	3	Mouse	8.9	pKi	=	8.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	24797604	109622	2	None	-	1	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	CHEMBL3236552	109622	2	None	-	1	Human	8.9	pKi	=	8.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	1	5	1.5	CN1CCN(c2cc(C3CCCC3)nc(N)n2)CC1	10.1021/jm401727m
	44231618	5798	0	None	1	4	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1079516	5798	0	None	1	4	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	25128824	192565	0	None	3	2	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192565	0	None	3	2	Rat	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	24992077	109641	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236571	109641	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	5	2	5	1.2	CCCCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	44231618	5798	0	None	-1	4	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1079516	5798	0	None	-1	4	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	287	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	44129130	197865	0	None	2	2	Rat	8.8	pKi	=	8.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	197865	0	None	2	2	Rat	8.8	pKi	=	8.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1223	938	32	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	938	32	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	938	32	None	-60	11	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	44187557	193817	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202332	193817	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL553423	193817	0	None	3	2	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	342	6	3	3	4.1	N=C(NCc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1893	114	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	1247	1893	114	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	1375	1893	114	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	774	1893	114	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	CHEMBL90	1893	114	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	DB05381	1893	114	None	-3	25	Human	8.8	pKi	=	8.8	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2016.09.049
	11957573	1971	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	1250	1971	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	3692	1971	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	CHEMBL19439	1971	29	None	-40	6	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2011.03.046
	16202258	67861	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915536	67861	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	264	4	3	6	0.9	CNC1CN(c2cc(NCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	66740859	109649	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	327	3	2	5	2.3	CN[C@@H]1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236579	109649	0	None	-	1	Human	8.8	pKi	=	8.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	327	3	2	5	2.3	CN[C@@H]1CCN(c2cc(C34CC5CC(CC(C5)C3)C4)nc(N)n2)C1	10.1021/jm401727m
	24798232	109630	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	1.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236560	109630	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	1.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCCN[C@H]3C2)n1	10.1021/jm401727m
	44231735	7688	0	None	1	4	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089390	7688	0	None	1	4	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	25129527	186821	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	186821	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	135525668	67855	0	None	263	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915348	67855	0	None	263	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	25217643	111665	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysis	ChEMBL	289	2	0	5	1.9	CN1CCN(c2ncnc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290570	111665	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor after 45 mins by scintillation counting analysis	ChEMBL	289	2	0	5	1.9	CN1CCN(c2ncnc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1249	1970	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	13499360	1970	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90019	1970	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10176414	3988	13	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1246	3988	13	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL281576	3988	13	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10060212	202819	0	None	5	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72769	202819	0	None	5	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CCC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681807	89202	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	8	3	8	1.6	CC(C)CCc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375668	89202	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	8	3	8	1.6	CC(C)CCc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681804	89227	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1ccnc(N)n1)c1cc(-c2ccccc2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375770	89227	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1ccnc(N)n1)c1cc(-c2ccccc2)nc(N)n1	10.1021/jm301886t
	1223	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1223	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1249	1970	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	13499360	1970	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	CHEMBL90019	1970	14	None	-3	3	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm0504353
	53233802	61203	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770987	61203	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	3	2	6	1.2	CN1CCN(c2cc(N)nc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	70692529	75316	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	303	2	1	2	2.7	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047466	75316	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	303	2	1	2	2.7	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	46228994	197738	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	466	6	1	7	2.7	CC1CCCCN1S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591249	197738	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	466	6	1	7	2.7	CC1CCCCN1S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	1223	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	2790	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	CHEMBL14690	938	32	None	-60	11	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2009.04.007
	45268092	194330	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1743881	194330	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560111	194330	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	352	6	3	3	3.6	N=C(NCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45268093	194331	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202321	194331	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560112	194331	0	None	-3	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	400	6	3	3	3.4	N=C(NCc1ccc(I)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45267230	194798	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202318	194798	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL563342	194798	0	None	-1	2	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1ccccc1Cl)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	24737954	67772	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(F)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914778	67772	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(F)c23)C1	10.1016/j.bmcl.2011.08.014
	24826522	67779	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914786	67779	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	24826168	67782	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914789	67782	0	None	-	1	Human	8.0	pKi	=	8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24745335	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	4	5	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	71525680	89287	0	None	97	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	284	3	2	6	1.3	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376806	89287	0	None	97	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	284	3	2	6	1.3	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	71525678	89281	0	None	5	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89281	0	None	5	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25101945	192494	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192494	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	136192886	56171	0	None	-1	4	Mouse	7.0	pKi	=	7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56171	0	None	-1	4	Mouse	7.0	pKi	=	7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	44129134	197817	0	None	-22	2	Rat	7.0	pKi	=	7	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	197817	0	None	-22	2	Rat	7.0	pKi	=	7	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11694543	75432	4	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	6	2	2	1.3	CCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204843	75432	4	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	6	2	2	1.3	CCNCCCCc1c[nH]cn1	10.1021/jm0504353
	11521414	140753	0	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	2	2	2.7	c1nc(CCCCNC2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL383324	140753	0	None	-3	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	2	2	2.7	c1nc(CCCCNC2CCCCC2)c[nH]1	10.1021/jm0504353
	9812843	203523	1	None	-4	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1016/j.bmcl.2011.06.123
	CHEMBL78838	203523	1	None	-4	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1016/j.bmcl.2011.06.123
	53233904	61181	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770965	61181	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	2	1	5	1.0	CN1CCN(c2cc(N)nc(N3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	39732699	75313	2	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	1	2	2	1.4	O=C(c1cc2cccc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047463	75313	2	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	1	2	2	1.4	O=C(c1cc2cccc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	70696623	75321	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	2	2	2	2.0	CNC1CN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047471	75321	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	2	2	2	2.0	CNC1CN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	51354298	58564	2	None	1	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	233	4	1	4	1.9	CC(C)(C)c1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688944	58564	2	None	1	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	233	4	1	4	1.9	CC(C)(C)c1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	45267237	194518	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202320	194518	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL561451	194518	0	None	-7	2	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	7	3	3	3.2	Cc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	53378933	109643	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	269	3	2	5	1.2	Nc1nc(Cc2ccccc2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236573	109643	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	269	3	2	5	1.2	Nc1nc(Cc2ccccc2)cc(N2CC[C@@H](N)C2)n1	10.1021/jm401727m
	11087453	75277	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL2047270	75277	0	None	-	1	Human	7.0	pKi	=	7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1039/C2MD20212C
	44395335	66712	0	None	25	2	Human	6.0	pKi	=	6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	266	4	2	3	1.5	CN(C)CCNC(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL187210	66712	0	None	25	2	Human	6.0	pKi	=	6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	266	4	2	3	1.5	CN(C)CCNC(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	10978141	5066	1	None	-4	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL105803	5066	1	None	-4	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	71681477	89214	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	398	7	4	8	3.3	Nc1nc(NCCNc2cc(-c3ccccc3)nc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375758	89214	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	398	7	4	8	3.3	Nc1nc(NCCNc2cc(-c3ccccc3)nc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	71681802	89225	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1cc(NCCNc2ccnc(N)n2)ncn1	10.1021/jm301886t
	CHEMBL2375769	89225	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1cc(NCCNc2ccnc(N)n2)ncn1	10.1021/jm301886t
	206876	18741	7	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL128637	18741	7	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	66572107	75272	1	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047265	75272	1	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	66572115	75292	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047442	75292	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	11469514	103678	2	None	-64	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL309654	103678	2	None	-64	2	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cc(CCCc2c[nH]cn2)ccn1	10.1021/jm049932u
	46890591	6932	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	436	6	1	4	4.8	Cc1cc(OCCCN2CC3CCCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084811	6932	0	None	-	1	Human	6.0	pKi	=	6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	436	6	1	4	4.8	Cc1cc(OCCCN2CC3CCCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	44275807	98500	0	None	-9120	10	Human	5.0	pKi	=	5	Binding	Binding affinity towards human histaminergic (H4) receptorBinding affinity towards human histaminergic (H4) receptor	ChEMBL	422	7	0	4	4.5	C[C@H]1CC[C@H](C)N1CCCOc1ccc(-c2ccc(C(=O)N3CCOCC3)cc2)cc1	10.1016/s0960-894x(03)00118-5
	CHEMBL27979	98500	0	None	-9120	10	Human	5.0	pKi	=	5	Binding	Binding affinity towards human histaminergic (H4) receptorBinding affinity towards human histaminergic (H4) receptor	ChEMBL	422	7	0	4	4.5	C[C@H]1CC[C@H](C)N1CCCOc1ccc(-c2ccc(C(=O)N3CCOCC3)cc2)cc1	10.1016/s0960-894x(03)00118-5
	4242418	116916	11	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	2	1	2	1.9	CN1CCN(Cc2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL339826	116916	11	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	2	1	2	1.9	CN1CCN(Cc2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	71681978	89209	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	310	5	3	5	1.7	CN(CCNc1ccnc(N)n1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm301886t
	CHEMBL2375753	89209	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	310	5	3	5	1.7	CN(CCNc1ccnc(N)n1)C(=O)c1cc2ccccc2[nH]1	10.1021/jm301886t
	58227158	116620	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1cc(N2CCN(C)CC2)cnn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393549	116620	0	None	-	1	Human	5.0	pKi	=	5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1cc(N2CCN(C)CC2)cnn1	10.1016/j.bmcl.2014.12.027
	57391926	69544	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938967	69544	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11846300	79841	3	None	-2	4	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL213912	79841	3	None	-2	4	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2015.12.035
	24854583	95663	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	304	2	0	4	3.0	CN1CCN(c2nc3ccccc3nc2-c2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260372	95663	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	304	2	0	4	3.0	CN1CCN(c2nc3ccccc3nc2-c2ccccc2)CC1	10.1021/jm7014217
	16082860	138069	0	None	-7	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL377572	138069	0	None	-7	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	57391032	67743	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@H]3C[C@@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914749	67743	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@H]3C[C@@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25179297	173249	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL453877	173249	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	24759518	178719	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL473302	178719	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178342	186194	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489070	186194	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179297	173249	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL453877	173249	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3nccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25178342	186194	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489070	186194	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	385	4	1	5	3.8	CN1CCN(c2nc(NCc3ccc(F)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	71518535	86046	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	1	5	2.6	CN1CCN(c2nc(N(C)C)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314763	86046	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	1	5	2.6	CN1CCN(c2nc(N(C)C)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	10978141	5066	1	None	-4	2	Human	6.0	pKi	=	6.0	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL105803	5066	1	None	-4	2	Human	6.0	pKi	=	6.0	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	57393730	67527	0	None	-2754	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	579	8	2	6	5.0	O=C(c1cc2ccccc2[nH]1)N1CCN(CCN2CCC(Nc3nc4ccccc4n3Cc3ccc(F)cc3)CC2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910386	67527	0	None	-2754	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	579	8	2	6	5.0	O=C(c1cc2ccccc2[nH]1)N1CCN(CCN2CCC(Nc3nc4ccccc4n3Cc3ccc(F)cc3)CC2)CC1	10.1016/j.bmcl.2011.09.001
	24826681	67762	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914768	67762	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.0	Nc1nc(N2CCNCC2)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	127049855	139685	0	None	-1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805220	139685	0	None	-1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	57391869	62775	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774589	62775	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789349	62775	0	None	-19	3	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	6	2	3	1.6	CC=CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	90645163	111685	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1cc(C)cc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290590	111685	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1cc(C)cc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	10656971	16229	4	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL123137	16229	4	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1016/j.bmcl.2011.07.114
	121238070	165923	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	4	1	7	1.4	CN1CCN(c2nc(N)nc(COc3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4278404	165923	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	4	1	7	1.4	CN1CCN(c2nc(N)nc(COc3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178555	173657	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454891	173657	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178555	173657	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454891	173657	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	374	4	1	7	3.1	CN1CCN(c2nc(NCc3cncs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	155540201	172329	0	None	-67	5	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	513	16	2	4	5.5	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc(Cl)cc1	10.1039/C8MD00527C
	CHEMBL4516173	172329	0	None	-67	5	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	513	16	2	4	5.5	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc(Cl)cc1	10.1039/C8MD00527C
	1278	2110	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	-281	8	Guinea pig	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	832283	12277	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL118572	12277	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	832283	12277	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL118572	12277	6	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc(NCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	10269628	138174	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	355	1	0	4	4.6	CC(C)N1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL377985	138174	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	355	1	0	4	4.6	CC(C)N1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	54582866	61204	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(N)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770988	61204	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(N)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2011.03.017
	25133970	186995	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL494679	186995	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	1	1	6	0.5	CN1CCN(c2nc(N)nc(C(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	49841962	69370	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3c(-c4ccoc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935562	69370	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3c(-c4ccoc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	44547568	116601	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393530	116601	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	13731320	158092	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	2	2	2	4.0	O=C(Nc1cccc2ccccc12)N1CCC(c2c[nH]cn2)CC1	10.1016/j.ejmech.2016.09.074
	CHEMBL4092578	158092	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	2	2	2	4.0	O=C(Nc1cccc2ccccc12)N1CCC(c2c[nH]cn2)CC1	10.1016/j.ejmech.2016.09.074
	127035837	136879	0	None	-5	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	403	4	1	5	3.7	CN1CCN(c2nc(NCc3ccc(F)cc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753334	136879	0	None	-5	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	403	4	1	5	3.7	CN1CCN(c2nc(NCc3ccc(F)cc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	21842421	111666	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	270	2	1	6	0.9	CN1CCN(c2nc(N)nc(-c3ccccc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290571	111666	2	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	270	2	1	6	0.9	CN1CCN(c2nc(N)nc(-c3ccccc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10688021	155558	0	None	-1	2	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	332	8	1	3	4.3	O=C(/C=C/c1ccccc1)c1ccc(OCCCc2c[nH]cn2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4063170	155558	0	None	-1	2	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	332	8	1	3	4.3	O=C(/C=C/c1ccccc1)c1ccc(OCCCc2c[nH]cn2)cc1	10.1016/j.ejmech.2016.09.074
	9944571	116503	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL338937	116503	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9944571	116503	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338937	116503	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	23650961	3773	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	9276	3773	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	CHEMBL3301609	3773	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	DB12522	3773	18	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	nan
	24803710	149784	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	400	6	2	5	4.1	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(F)c(F)ccc2[nH]1	nan
	CHEMBL3953011	149784	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	400	6	2	5	4.1	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(F)c(F)ccc2[nH]1	nan
	25101945	192494	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192494	0	None	-3	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	118720437	115400	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	275	3	2	5	1.0	CN[C@@H]1CCN(c2cc(CC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354811	115400	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	275	3	2	5	1.0	CN[C@@H]1CCN(c2cc(CC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	24737952	67777	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(OC)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914784	67777	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(OC)cc23)C1	10.1016/j.bmcl.2011.08.014
	9944571	116503	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338937	116503	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3cc(Cl)cc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	24996854	109634	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	CHEMBL3236564	109634	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	2	5	2.0	Nc1nc(C2CCCC2)cc(N2CCC3(CCCN3)C2)n1	10.1021/jm401727m
	71603142	89277	0	None	54	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	3	2	5	1.2	CCc1ccc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376796	89277	0	None	54	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	3	2	5	1.2	CCc1ccc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	44129282	197510	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)cc(Cl)c3n2)CC1	10.1021/jm901379s
	CHEMBL589746	197510	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)cc(Cl)c3n2)CC1	10.1021/jm901379s
	71603139	89274	0	None	13	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	2	2	5	1.5	Nc1nc(N2CCNCC2)c2ccc(C3CC3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376793	89274	0	None	13	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	2	2	5	1.5	Nc1nc(N2CCNCC2)c2ccc(C3CC3)cc2n1	10.1016/j.bmcl.2013.02.091
	45257132	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	155513612	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	44450493	96074	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	1	1	4	1.3	CN1CCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL262859	96074	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	1	1	4	1.3	CN1CCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	1278	2110	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-4	8	Mouse	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	126963283	160404	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL4115677	160404	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	1224	1399	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	3100	1399	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	8980	1399	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	916	1399	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	CHEMBL657	1399	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	DB01075	1399	79	None	-194	13	Human	5.0	pKi	=	5.0	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	nan
	1241	1998	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	1242	1998	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	3614	1998	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	CHEMBL507	1998	46	None	-89	5	Human	5.0	pKi	=	5.0	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	125	2	1	3	-0.1	Cn1cc(nc1)CCN	10.1016/j.bmcl.2016.09.049
	49841609	69364	13	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3c(Br)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935556	69364	13	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3c(Br)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	51354871	58582	0	None	-22	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	290	6	0	4	3.3	c1c(CC2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1688982	58582	0	None	-22	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	290	6	0	4	3.3	c1c(CC2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	164618508	185340	0	None	-363	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	6	4	4	2.7	Cc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4869194	185340	0	None	-363	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	6	4	4	2.7	Cc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	164620678	185427	0	None	-177	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.7	CCC(NC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4870510	185427	0	None	-177	4	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.7	CCC(NC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	58227159	175801	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4534254	175801	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595907	175801	0	None	47	2	Human	6.9	pKi	=	6.9	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	1	5	1.7	CN1CCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	122193099	123387	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	374	3	1	6	2.1	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Br)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628046	123387	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	374	3	1	6	2.1	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Br)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	51354008	58559	0	None	-3	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	253	5	1	4	1.8	c1ccc(Cc2cn(CCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688939	58559	0	None	-3	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	253	5	1	4	1.8	c1ccc(Cc2cn(CCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	51354586	58579	0	None	-15	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	2.9	CC(c1cn(CCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	CHEMBL1688978	58579	0	None	-15	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	2.9	CC(c1cn(CCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	16082824	77579	0	None	1	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL209598	77579	0	None	1	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	54585778	61205	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(N)nc(NCCO)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770989	61205	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(N)nc(NCCO)n2)CC1	10.1016/j.bmcl.2011.03.017
	58227165	116607	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393536	116607	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	1	5	1.0	CC(C)Nc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	24853664	95881	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261517	95881	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	10773349	7992	1	None	-11	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL109130	7992	1	None	-11	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	10715857	74651	1	None	-3	3	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	398	8	1	3	3.5	O=C(CCCc1ccc(I)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL20338	74651	1	None	-3	3	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	398	8	1	3	3.5	O=C(CCCc1ccc(I)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	16082820	79693	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213250	79693	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	261	5	2	2	3.0	Clc1ccc(CNC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	10776393	5407	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1076267	5407	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL156912	5407	0	None	-25	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	239	7	2	3	2.1	CC[C@H](C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	118725542	116608	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393537	116608	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	1278	2110	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2110	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2110	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2110	79	None	-3	8	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	90645161	111683	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3cc(F)cc(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290588	111683	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3cc(F)cc(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	1278	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	45257132	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	8983	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	CHEMBL595180	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	10.1016/j.bmc.2009.08.059
	24994634	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	24994634	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	8984	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1016/j.bmcl.2014.12.027
	CHEMBL3236549	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	24994635	109640	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236570	109640	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(C(C)C)nc(N)n2)C1	10.1021/jm401727m
	24826442	67750	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1ccc2oc3c(N4CCN(C)CC4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914756	67750	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1ccc2oc3c(N4CCN(C)CC4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	1278	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1278	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1204	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1247	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1375	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	774	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	DB05381	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm1013488
	1278	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1279	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	4908365	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1204	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1204	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	1247	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1247	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	1375	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	1375	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	774	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	774	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	DB05381	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014217
	DB05381	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm901379s
	155513612	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	1226	1980	16	None	2	7	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	41376	1980	16	None	2	7	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	CHEMBL12608	1980	16	None	2	7	Human	7.9	pKi	=	7.9	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1021/jm0341047
	24745335	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	71603135	89270	0	None	26	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	2	2	5	2.3	Nc1nc(N2CCNCC2)c2ccc(C3CCCC3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376789	89270	0	None	26	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	2	2	5	2.3	Nc1nc(N2CCNCC2)c2ccc(C3CCCC3)cc2n1	10.1016/j.bmcl.2013.02.091
	71525589	89288	0	None	11	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	286	3	2	6	1.5	CN[C@@H]1CCN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376807	89288	0	None	11	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	286	3	2	6	1.5	CN[C@@H]1CCN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	24745335	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	-4	5	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	24994634	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	8984	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	CHEMBL3236549	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Binding assayBinding assay	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.6019/CHEMBL5058644
	24994634	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	3035746	187587	6	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL1237146	187587	6	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	CHEMBL498770	187587	6	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human H4RBinding affinity to human H4R	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.9b01342
	1204	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	155513612	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175736	0	None	1	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1249	1970	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	13499360	1970	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90019	1970	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1253	2019	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	132937	2019	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19010	2019	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10023327	168439	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168439	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor Q7.42L mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	10023327	168439	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168439	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	10023327	168439	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168439	0	None	-144	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T6.55M mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	1253	2019	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	132937	2019	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL19010	2019	7	None	-11	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	10.1021/jm201042n
	1204	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	1247	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	1375	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	774	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	DB05381	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm060880d
	11065080	19221	2	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL129488	19221	2	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	66573460	75301	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.2	Cc1cc(Cl)cc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047451	75301	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.2	Cc1cc(Cl)cc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	21638785	188063	4	None	-4	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL503605	188063	4	None	-4	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	44129132	197843	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	6	1	7	1.3	CN1CCN(c2nc(NCCS(=O)(=O)N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592188	197843	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	6	1	7	1.3	CN1CCN(c2nc(NCCS(=O)(=O)N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10176414	3988	13	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	1246	3988	13	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL281576	3988	13	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	184	4	3	3	1.0	NC(=N)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	45267233	193228	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202319	193228	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL541171	193228	0	None	-1	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	304	7	3	4	2.8	COc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	1278	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1249	1970	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	13499360	1970	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL90019	1970	14	None	-3	3	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	25128823	192105	0	None	-2	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192105	0	None	-2	2	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	127050431	139772	0	None	4	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139772	0	None	4	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11451949	67688	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	384	6	1	4	3.9	CN1CCN(CCCOc2ccc(-c3nc4cc(Cl)ccc4[nH]3)cc2)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914533	67688	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	384	6	1	4	3.9	CN1CCN(CCCOc2ccc(-c3nc4cc(Cl)ccc4[nH]3)cc2)CC1	10.1016/j.bmcl.2011.08.014
	46228126	200916	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.4	CN1CCN(c2cc(NCc3ccc(C(F)(F)F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL612131	200916	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.4	CN1CCN(c2cc(NCc3ccc(C(F)(F)F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	60150166	174142	0	None	-323	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	524	17	2	6	4.7	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc([N+](=O)[O-])cc1	10.1039/C8MD00527C
	CHEMBL4560123	174142	0	None	-323	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	524	17	2	6	4.7	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccc([N+](=O)[O-])cc1	10.1039/C8MD00527C
	168295543	192429	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209021	192429	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222929	192429	0	None	-8	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	380	4	4	4	3.3	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	164610648	184245	0	None	-120	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4852212	184245	0	None	-120	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	10820930	5707	0	None	1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL107877	5707	0	None	1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@H]1C=C[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	24826443	67764	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914770	67764	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	10800875	6188	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1081718	6188	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL345828	6188	0	None	-75	3	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	24745335	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from guinea pig H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	-22	5	Guinea pig	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from guinea pig histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	1223	938	32	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-363	11	Mouse	6.9	pKi	=	6.9	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	10800875	6188	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1081718	6188	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL345828	6188	0	None	-75	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	253	8	2	3	2.5	CCC(C)CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	11206058	103923	9	None	-10	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL310360	103923	9	None	-10	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1ccc(CCc2c[nH]cn2)nc1	10.1021/jm049932u
	1267	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3035905	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	CHEMBL260374	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0603318
	3062060	10268	5	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	221	4	6	3	-1.0	N=C(N)NCc1cccc(CNC(=N)N)n1	10.1021/jm7014777
	CHEMBL1162346	10268	5	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	221	4	6	3	-1.0	N=C(N)NCc1cccc(CNC(=N)N)n1	10.1021/jm7014777
	11511026	15845	0	None	-208	4	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3ccc4ccccc4n3)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	CHEMBL1223683	15845	0	None	-208	4	Human	6.9	pKi	=	6.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3ccc4ccccc4n3)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	1267	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	3035905	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	CHEMBL260374	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	10106063	108770	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting method	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	CHEMBL321860	108770	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins by liquid scintillation counting method	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/acs.jmedchem.6b00120
	24826000	67786	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	2	2	6	3.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914793	67786	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	2	2	6	3.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)C1	10.1016/j.bmcl.2011.08.014
	1267	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	3035905	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	CHEMBL260374	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	1278	2110	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-4	8	Mouse	6.9	pKi	=	6.9	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10106063	108770	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321860	108770	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	9974306	202759	0	None	-15	4	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72372	202759	0	None	-15	4	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	336	8	3	2	3.4	S=C(NCCCCCc1c[nH]cn1)NCc1ccc(Cl)cc1	10.1016/j.bmcl.2010.10.041
	56849569	71253	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923033	71253	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963332	71253	0	None	-77	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	381	7	2	4	4.4	N#Cc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11499453	75843	0	None	-79	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	2	2	2.4	CC(C)CNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205834	75843	0	None	-79	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	2	2	2.4	CC(C)CNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11672850	76305	2	None	-10	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.1	CC(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206451	76305	2	None	-10	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.1	CC(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	1267	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	3035905	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	CHEMBL260374	3732	43	None	-33	28	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.04.007
	10106063	108770	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321860	108770	6	None	16	2	Human	6.9	pKi	=	6.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	5310982	93166	4	None	-1348	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	CHEMBL24665	93166	4	None	-1348	3	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	255	3	2	2	2.6	NCCc1c[nH]c(-c2cccc(C(F)(F)F)c2)n1	10.1016/j.bmcl.2010.10.041
	71681975	89206	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	296	5	4	5	1.4	Nc1nccc(NCCNC(=O)c2cc3ccccc3[nH]2)n1	10.1021/jm301886t
	CHEMBL2375750	89206	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	296	5	4	5	1.4	Nc1nccc(NCCNC(=O)c2cc3ccccc3[nH]2)n1	10.1021/jm301886t
	71681642	89221	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nc(NCCNc2ccnc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375765	89221	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nc(NCCNc2ccnc(N)n2)cc(-c2ccccc2)n1	10.1021/jm301886t
	11286909	203563	2	None	-1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL79126	203563	2	None	-1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1ccc(CCCc2c[nH]cn2)nc1	10.1021/jm049932u
	10678661	5275	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106931	5275	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	11043445	56170	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632410	56170	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	168294449	192406	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204599	192406	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222793	192406	0	None	-181	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	367	7	4	5	1.9	CC(CNC(=O)/N=C(\N)NCCCc1nnc(N)s1)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	66572111	75278	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047271	75278	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	118720435	115399	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	225	3	2	5	0.3	CN[C@@H]1CCN(c2cc(CF)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354809	115399	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	225	3	2	5	0.3	CN[C@@H]1CCN(c2cc(CF)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	90645151	111667	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290572	111667	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2014.06.032
	16082833	79922	0	None	-23	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214312	79922	0	None	-23	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	54584826	61209	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770993	61209	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	57395538	67854	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915347	67854	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	57395538	67854	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915347	67854	0	None	151	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	25178343	172012	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL448962	172012	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178343	172012	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL448962	172012	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	392	4	1	6	3.5	CN1CCN(c2nc(NCc3ccc(C#N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	57403269	67760	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	1	6	2.5	CN1CCN(c2nc(N)nc3c4cc(Cl)ccc4[s+]([O-])c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914766	67760	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	1	6	2.5	CN1CCN(c2nc(N)nc3c4cc(Cl)ccc4[s+]([O-])c23)CC1	10.1016/j.bmcl.2011.08.014
	24854705	95652	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	3	0	6	2.6	CN1CCN(c2nc3ccccc3nc2Oc2cccnc2)CC1	10.1021/jm7014217
	CHEMBL260274	95652	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	3	0	6	2.6	CN1CCN(c2nc3ccccc3nc2Oc2cccnc2)CC1	10.1021/jm7014217
	164619208	185070	0	None	-446	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccccc1F	10.1016/j.ejmech.2021.113190
	CHEMBL4864852	185070	0	None	-446	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccccc1F	10.1016/j.ejmech.2021.113190
	10678661	5275	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106931	5275	1	None	-61	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	57402929	69509	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938844	69509	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1278	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	1279	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	4908365	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	57392827	67766	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914772	67766	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.4	Nc1nc(N2CCNCC2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	58227163	175879	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4518374	175879	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596491	175879	0	None	40	2	Human	7.9	pKi	=	7.9	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	3	2	5	1.7	CC(C)(C)CNc1nccc(N2CCCNCC2)n1	10.1021/acs.jmedchem.9b01342
	1204	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	1247	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	1375	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	774	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	DB05381	1893	114	None	-3	25	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm7014777
	16082856	80686	0	None	-2	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL215628	80686	0	None	-2	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	46929647	16564	0	None	-91	3	Human	5.9	pKi	=	5.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	CHEMBL1243394	16564	0	None	-91	3	Human	5.9	pKi	=	5.9	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	11858011	158445	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	9	0	3	4.6	O=C(c1ccccc1)c1ccc(OCCCCCN2CCCC2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4096444	158445	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	9	0	3	4.6	O=C(c1ccccc1)c1ccc(OCCCCCN2CCCC2)cc1	10.1016/j.ejmech.2016.09.074
	16352	116670	11	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL339414	116670	11	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	293	0	0	4	3.1	CN1CCN(C2=Nc3ccccc3Oc3ccccc32)CC1	10.1021/jm051008s
	11240759	104721	5	None	-190	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	CHEMBL311473	104721	5	None	-190	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cc(CCc2c[nH]cn2)ccn1	10.1021/jm049932u
	24853662	95616	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	3	0	5	3.1	CN1CCN(c2nc3ccccc3nc2OC2CCCCC2)CC1	10.1021/jm7014217
	CHEMBL260043	95616	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	3	0	5	3.1	CN1CCN(c2nc3ccccc3nc2OC2CCCCC2)CC1	10.1021/jm7014217
	54584378	62814	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774595	62814	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789782	62814	0	None	-50	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164626153	185786	0	None	-6456	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccc(F)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4875600	185786	0	None	-6456	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccc(F)c1	10.1016/j.ejmech.2021.113190
	54583874	61202	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N)nc(N3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770986	61202	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N)nc(N3CCCC3)n2)CC1	10.1016/j.bmcl.2011.03.017
	54584378	62814	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774595	62814	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789782	62814	0	None	-50	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CC/C=C/CCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	46890122	7277	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	421	9	2	5	4.3	Cc1cc(OCCCNCCc2cn(C)cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086344	7277	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	421	9	2	5	4.3	Cc1cc(OCCCNCCc2cn(C)cn2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	155568016	175454	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	4	1	6	1.1	CCC(CC)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4590367	175454	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	4	1	6	1.1	CCC(CC)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	58227319	116611	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393540	116611	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	56849571	71254	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923034	71254	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963347	71254	0	None	-117	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	401	8	2	5	4.5	O=[N+]([O-])c1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	25179126	172831	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm800876b
	CHEMBL452849	172831	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm800876b
	25179126	172831	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm901379s
	CHEMBL452849	172831	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)o1	10.1021/jm901379s
	164609729	184505	0	None	-2187	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856059	184505	0	None	-2187	6	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	164622886	185350	0	None	-131	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4869389	185350	0	None	-131	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	374	7	4	4	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	164626509	185743	0	None	-3	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	358	3	4	4	3.2	N/C(=N\C(=O)NC1CCCCC1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4874969	185743	0	None	-3	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	358	3	4	4	3.2	N/C(=N\C(=O)NC1CCCCC1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	11043445	56170	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL1632410	56170	1	None	-1	2	Human	4.9	pKi	=	4.9	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	1204	1893	114	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1893	114	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1893	114	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1893	114	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1893	114	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1893	114	None	-12	25	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	11609325	14346	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.3	CN1CCN(C(=O)c2nc3c(Cl)cc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL120221	14346	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.3	CN1CCN(C(=O)c2nc3c(Cl)cc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	24825796	67738	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	NC[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914744	67738	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	NC[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	46228100	198323	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	315	4	1	6	2.1	CN1CCN(c2cc(SCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595413	198323	0	None	-	1	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	315	4	1	6	2.1	CN1CCN(c2cc(SCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	44628631	67767	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3c(Cl)cc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914773	67767	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3c(Cl)cc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	71525679	89282	0	None	-9	6	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89282	0	None	-9	6	Rat	6.9	pKi	=	6.9	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	4060730	19168	51	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL129199	19168	51	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	24853594	95678	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	4	0	5	3.3	Cc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL260470	95678	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	4	0	5	3.3	Cc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	44414495	79594	0	None	-34	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	5	0	2	6.3	CC1CC(C)CN(Cc2ccc(-c3ccc(CN4CC(C)CC(C)C4)cc3)cc2)C1	10.1016/j.bmc.2008.10.029
	CHEMBL212869	79594	0	None	-34	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	5	0	2	6.3	CC1CC(C)CN(Cc2ccc(-c3ccc(CN4CC(C)CC(C)C4)cc3)cc2)C1	10.1016/j.bmc.2008.10.029
	127026017	136820	0	None	-12	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752972	136820	0	None	-12	2	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)cc1	10.1016/j.bmcl.2015.12.035
	164614907	183991	0	None	-436	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(F)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848697	183991	0	None	-436	4	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	378	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(F)cc1	10.1016/j.ejmech.2021.113190
	57390263	67830	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	330	1	2	7	1.8	Nc1nc(N2CC3CCNC3C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915052	67830	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	330	1	2	7	1.8	Nc1nc(N2CC3CCNC3C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	9900313	19211	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129444	19211	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9900313	19211	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129444	19211	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0341047
	1278	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2110	79	None	-3	8	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	46890592	6300	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082296	6300	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@H]3CCCN[C@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	9900313	19211	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129444	19211	0	None	-	1	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3cc(F)cc(F)c3[nH]2)CC1	10.1021/jm0502081
	156013896	177655	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4635634	177655	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650570	177655	0	None	-7	4	Human	7.9	pKi	=	7.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	401	9	1	2	5.1	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25129523	186694	40	None	-1	2	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	186694	40	None	-1	2	Rat	7.9	pKi	=	7.9	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	1249	1970	14	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	13499360	1970	14	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	CHEMBL90019	1970	14	None	-3	3	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	139	4	2	2	0.7	NCCCCc1cnc[nH]1	10.1021/jm049932u
	1204	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1247	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	1375	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	774	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	CHEMBL90	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	DB05381	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Inhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptorInhibition of [3H]-histamine binding to membranes of SK-N-MC cells expressing the human Histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm030905y
	137655927	158330	0	None	17	2	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	403	7	2	3	5.2	O=C(NC(c1ccc(Cl)cc1)c1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL4095224	158330	0	None	17	2	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	403	7	2	3	5.2	O=C(NC(c1ccc(Cl)cc1)c1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	71603141	89276	0	None	14	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	5	1.2	CCc1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376795	89276	0	None	14	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	5	1.2	CCc1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	54585779	61207	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770991	61207	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25179303	188747	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCCC3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL512568	188747	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCCC3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	51354304	58573	0	None	-3	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCn2cc(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688972	58573	0	None	-3	2	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCn2cc(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	71519016	86028	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314501	86028	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	71518859	86054	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	294	2	3	5	1.6	Nc1nc(N2CCNCC2)c2cc(-c3ccccc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314772	86054	0	None	-	1	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	294	2	3	5	1.6	Nc1nc(N2CCNCC2)c2cc(-c3ccccc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	156014317	177692	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634728	177692	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650854	177692	0	None	1	2	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	575	10	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/C=C/c3ccc(N(C)C)cc3)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	11724853	96559	0	None	-19	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	340	0	0	4	3.7	CN1CCN(C2=Nc3cc(Cl)ccc3N(C)c3ccccc32)CC1	10.1021/jm051008s
	CHEMBL266723	96559	0	None	-19	5	Human	5.9	pKi	=	5.9	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	340	0	0	4	3.7	CN1CCN(C2=Nc3cc(Cl)ccc3N(C)c3ccccc32)CC1	10.1021/jm051008s
	57398985	67526	0	None	-1122	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	569	12	2	7	4.4	COc1ccc(CN(CCN(C)CC(C)Nc2ccc3[nH]c(C(=O)N4CCN(C)CC4)cc3c2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910385	67526	0	None	-1122	3	Human	4.9	pKi	=	4.9	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	569	12	2	7	4.4	COc1ccc(CN(CCN(C)CC(C)Nc2ccc3[nH]c(C(=O)N4CCN(C)CC4)cc3c2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	118725539	116594	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	2	0	4	1.8	CN1CCN(c2ccnc(N3CCCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393523	116594	0	None	-	1	Human	6.9	pKi	=	6.9	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	2	0	4	1.8	CN1CCN(c2ccnc(N3CCCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	16082822	79555	0	None	-4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@@H]2C[C@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL212705	79555	0	None	-4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@@H]2C[C@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	46228160	198167	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	308	3	0	6	1.6	CN1CCN(c2ncnc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594468	198167	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	308	3	0	6	1.6	CN1CCN(c2ncnc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	2306318	86566	12	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1039/C5MD00022J
	CHEMBL232421	86566	12	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1039/C5MD00022J
	127036980	136809	0	None	-16	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752885	136809	0	None	-16	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	57391033	67763	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	287	1	2	6	1.5	Nc1nc(N2CCNCC2)c2oc3cccc(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914769	67763	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	287	1	2	6	1.5	Nc1nc(N2CCNCC2)c2oc3cccc(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	1236	2028	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	1237	2028	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	156615	2028	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	CHEMBL268229	2028	23	None	-204	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm0341047
	24797248	198174	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594490	198174	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	324	2	1	6	1.4	CN1CCN(c2cc(N3CCc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	9951970	5411	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1076300	5411	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL154797	5411	1	None	-81	3	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	121238066	166128	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	2	8	-1.0	CN1CCN(c2nc(N)nc(CN3C(=O)NC(C)(C)C3=O)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4282283	166128	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	2	8	-1.0	CN1CCN(c2nc(N)nc(CN3C(=O)NC(C)(C)C3=O)n2)CC1	10.1016/j.ejmech.2017.09.035
	10860228	116322	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338001	116322	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0341047
	10860228	116322	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338001	116322	1	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3ccc(Br)cc3[nH]2)CC1	10.1021/jm0502081
	3840250	136081	16	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	5	2.4	Cc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	CHEMBL3739776	136081	16	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	5	2.4	Cc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	56851447	71266	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1923026	71266	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1963433	71266	0	None	-144	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	424	7	2	3	5.9	Clc1ccc(C/N=C(/NC2CCCCC2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	90645157	111679	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1Cl	10.1016/j.ejmech.2014.06.032
	CHEMBL3290584	111679	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1Cl	10.1016/j.ejmech.2014.06.032
	2971762	192614	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL523617	192614	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	44450401	155578	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	3	2	4	2.0	O=c1[nH]c2ccc(Cl)cc2nc1NCN1CCCC1	10.1021/jm7014217
	CHEMBL406345	155578	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	278	3	2	4	2.0	O=c1[nH]c2ccc(Cl)cc2nc1NCN1CCCC1	10.1021/jm7014217
	2971762	192614	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL523617	192614	4	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	347	5	1	5	3.0	CN1CCN(c2nc(NCCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	164622190	185024	0	None	-93	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4864067	185024	0	None	-93	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCCc1ccccc1	10.1016/j.ejmech.2021.113190
	54586762	61183	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	4	2	5	2.0	CN1CCN(c2cc(N)nc(NCc3ccccc3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770967	61183	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	4	2	5	2.0	CN1CCN(c2cc(N)nc(NCc3ccccc3)c2)CC1	10.1016/j.bmcl.2011.03.017
	136066109	198740	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	46231557	198740	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	CHEMBL598291	198740	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	289	6	2	2	3.7	Cc1[nH]cnc1[C@@H]1C[C@H]1CCNCc1ccc(Cl)cc1	10.1016/j.bmc.2009.12.046
	5477514	10267	1	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	408	4	4	5	2.6	C/N=C(\N)N/N=C(/C)c1ccc2sc3ccc(/C(C)=N\N/C(N)=N/C)cc3c2c1	10.1021/jm7014777
	CHEMBL1162345	10267	1	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	408	4	4	5	2.6	C/N=C(\N)N/N=C(/C)c1ccc2sc3ccc(/C(C)=N\N/C(N)=N/C)cc3c2c1	10.1021/jm7014777
	24826083	67790	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914797	67790	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	10988959	117592	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL340740	117592	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	4906599	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL436381	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10988959	117592	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL340740	117592	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0341047
	4906599	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL436381	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0341047
	57391819	69378	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935570	69378	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)c1	10.1016/j.bmcl.2011.10.104
	53233801	61201	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770985	61201	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	10988959	117592	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL340740	117592	4	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	4906599	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL436381	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	71519674	89285	0	None	-1	5	Mouse	7.8	pKi	=	7.8	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89285	0	None	-1	5	Mouse	7.8	pKi	=	7.8	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	56835171	69382	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	CHEMBL1935574	69382	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2013.02.091
	71603138	89273	0	None	32	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	2	5	1.9	CNC1CN(c2nc(N)nc3cc(C(C)(C)C)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376792	89273	0	None	32	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	2	5	1.9	CNC1CN(c2nc(N)nc3cc(C(C)(C)C)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25128822	186888	0	None	-10	5	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	186888	0	None	-10	5	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	44129214	197512	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	538	7	2	7	3.7	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Br)c(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL589782	197512	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	538	7	2	7	3.7	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Br)c(Cl)cc3n2)CC1	10.1021/jm901379s
	136192874	56174	0	None	-1	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56174	0	None	-1	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	25147772	172827	4	None	1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	172827	4	None	1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	136192874	56174	0	None	-1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56174	0	None	-1	3	Rat	7.8	pKi	=	7.8	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1247	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1375	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	774	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	CHEMBL90	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	DB05381	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]-histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C5MD00022J
	1223	938	32	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	938	32	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	938	32	None	-60	11	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	1204	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1247	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1375	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	774	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL90	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	DB05381	1893	114	None	-3	25	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/jm201042n
	1278	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	1279	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	4908365	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	CHEMBL129198	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm201042n
	10967174	19227	3	None	-2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129540	19227	3	None	-2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70681963	75320	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	2	1	2	2.9	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047470	75320	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	2	1	2	2.9	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	4906599	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL436381	168086	10	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cc(F)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1278	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	1279	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	4908365	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	CHEMBL129198	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014217
	3035746	187587	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL1237146	187587	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL498770	187587	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	45269820	193669	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202326	193669	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL551790	193669	0	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	308	6	3	3	3.5	N=C(NCc1cccc(Cl)c1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1278	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	1279	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	4908365	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	CHEMBL129198	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm051008s
	155491015	175911	3	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175911	3	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175911	3	None	-15	4	Mouse	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]histamine from mouse H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	25129525	186990	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1039/C2MD20212C
	CHEMBL494648	186990	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	299	3	1	6	1.5	COc1ccc(-c2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1039/C2MD20212C
	46228125	198295	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595215	198295	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	366	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(Cl)c(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890020	7272	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	8	3	4	4.5	Cc1cc(OCCCNCC2CCCCN2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086327	7272	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	8	3	4	4.5	Cc1cc(OCCCNCC2CCCCN2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	42641407	197695	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(Cl)cc(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL591013	197695	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(Cl)cc(Cl)cc3n2)CC1	10.1021/jm901379s
	127036981	136912	0	None	-1	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753621	136912	0	None	-1	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)c3c(n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	135570464	178509	3	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	1	5	1.7	CN1CCN(c2nc(O)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL471712	178509	3	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	1	5	1.7	CN1CCN(c2nc(O)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354297	58563	0	None	-5	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	289	6	1	6	0.4	CN1CCN(Cc2cn(CCCc3c[nH]cn3)nn2)CC1	10.1021/jm1013488
	CHEMBL1688943	58563	0	None	-5	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	289	6	1	6	0.4	CN1CCN(Cc2cn(CCCc3c[nH]cn3)nn2)CC1	10.1021/jm1013488
	46890074	6510	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	403	8	1	3	6.2	Cc1cc(OCCCCCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083160	6510	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	403	8	1	3	6.2	Cc1cc(OCCCCCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	10107667	62820	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774599	62820	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789800	62820	0	None	-6	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	25178755	176290	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCC[C@H]3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL461537	176290	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	5	2.6	CNc1nc(N2CCN3CCC[C@H]3C2)nc2ccc(Cl)cc12	10.1021/jm800876b
	24759518	178719	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL473302	178719	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	277	1	1	5	1.6	CN1CCN(c2nc(N)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1267	3732	43	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	118720439	115406	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	246	2	2	4	2.2	Nc1nc(C2CCCC2)cc(C2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354817	115406	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	246	2	2	4	2.2	Nc1nc(C2CCCC2)cc(C2CCNCC2)n1	10.1016/j.bmcl.2014.10.013
	24853527	166862	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	4	0	6	3.2	CN1CCN(c2nc3ccccc3nc2Oc2cccc(N(C)C)c2)CC1	10.1021/jm7014217
	CHEMBL429691	166862	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	4	0	6	3.2	CN1CCN(c2nc3ccccc3nc2Oc2cccc(N(C)C)c2)CC1	10.1021/jm7014217
	10107667	62820	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774599	62820	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789800	62820	0	None	-6	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	279	7	2	3	3.0	O=C(NCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	10760516	62827	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774598	62827	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789829	62827	0	None	-9	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	127050431	139772	0	None	4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806206	139772	0	None	4	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	10760516	62827	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774598	62827	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789829	62827	0	None	-9	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	7	2	3	1.9	O=C(NCCC1CC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	57391034	67787	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914794	67787	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	57396299	67752	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	1	1	6	2.0	CN1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914758	67752	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	1	1	6	2.0	CN1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	57396300	67769	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	305	1	2	6	1.6	Nc1nc(N2CCNCC2)c2oc3ccc(F)c(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914775	67769	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	305	1	2	6	1.6	Nc1nc(N2CCNCC2)c2oc3ccc(F)c(F)c3c2n1	10.1016/j.bmcl.2011.08.014
	46228161	198208	12	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	233	1	2	6	-0.3	CN1CCN(c2nc(N)nc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594694	198208	12	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	233	1	2	6	-0.3	CN1CCN(c2nc(N)nc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	1223	938	32	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-363	11	Mouse	6.8	pKi	=	6.8	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	10023327	168439	0	None	-144	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168439	0	None	-144	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11615601	74655	0	None	-10	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	1	2	3.0	c1nc(CCCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203390	74655	0	None	-10	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	1	2	3.0	c1nc(CCCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	11600849	140001	0	None	-31	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL381074	140001	0	None	-31	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	66572108	75273	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3c(O)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047266	75273	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3c(O)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	9965450	75279	3	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3ccc(Cl)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047272	75279	3	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3ccc(Cl)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	45268970	194499	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202325	194499	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL561369	194499	0	None	-7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	318	8	3	4	2.9	COc1ccc(CCNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	45267238	194546	0	None	1	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	345	7	4	2	3.5	N=C(NC=C(c1ccccc1)c1ccccc1)NCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL561651	194546	0	None	1	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	345	7	4	2	3.5	N=C(NC=C(c1ccccc1)c1ccccc1)NCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1893	114	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	6.8	pKi	=	6.8	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	127050019	140221	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	233	2	2	5	0.3	CN[C@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1039/C6MD00108D
	CHEMBL3818355	140221	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	233	2	2	5	0.3	CN[C@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1039/C6MD00108D
	1194	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	23786499	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	CHEMBL26116	46	3	None	-1318	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	10.1016/j.bmcl.2010.10.041
	127035835	136748	0	None	1	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752270	136748	0	None	1	4	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	70690368	75311	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	1	1	2	2.3	Cc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047461	75311	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	1	1	2	2.3	Cc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24854707	95835	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	338	3	0	5	3.3	CN1CCN(c2nc3ccccc3nc2Oc2ccc(F)cc2)CC1	10.1021/jm7014217
	CHEMBL261222	95835	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	338	3	0	5	3.3	CN1CCN(c2nc3ccccc3nc2Oc2ccc(F)cc2)CC1	10.1021/jm7014217
	57390224	67520	0	None	-776	3	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	503	8	1	5	4.7	COc1ccc(CN(CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910379	67520	0	None	-776	3	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	503	8	1	5	4.7	COc1ccc(CN(CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	4905344	35094	9	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2cc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL1437043	35094	9	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1ccc2cc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1016/j.ejmech.2012.06.016
	70690367	75309	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	3	2.0	COc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047459	75309	0	None	-	1	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	3	2.0	COc1cccc2cc(C(=O)N3CCCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	59777309	109652	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.3	CN[C@@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1021/jm401727m
	CHEMBL3236581	109652	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.3	CN[C@@H]1CCN(c2nc(N)nc3c2CCC3)C1	10.1021/jm401727m
	57400777	67852	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915345	67852	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	56835171	69382	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69382	0	None	1	9	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	3035746	187587	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL1237146	187587	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	CHEMBL498770	187587	6	None	-1	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 minsDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cell membrane incubated for 60 mins	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/acs.jmedchem.6b00120
	53378928	115401	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	289	4	2	5	1.4	CN[C@@H]1CCN(c2cc(CCC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354812	115401	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	289	4	2	5	1.4	CN[C@@H]1CCN(c2cc(CCC(F)(F)F)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	44547564	116602	2	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	2.2	CN1CCN(c2ccnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393531	116602	2	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	2.2	CN1CCN(c2ccnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	57400777	67852	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915345	67852	0	None	426	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	1	2	2.5	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400738	67827	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.5	Nc1nc(N2CC[C@@H](N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915049	67827	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.5	Nc1nc(N2CC[C@@H](N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	56851445	71282	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923025	71282	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963551	71282	0	None	2	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	8	2	3	3.8	CCCCN/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	71603194	89278	0	None	35	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	3	2	5	1.6	CCc1ccc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376797	89278	0	None	35	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	3	2	5	1.6	CCc1ccc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	45257133	198212	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198212	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	155540438	171964	0	None	7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4483783	171964	0	None	7	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	290	3	1	6	1.3	CN1CCN(c2nc(N)nc(CC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	57394625	67960	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	325	7	2	6	1.7	COc1ccccc1Cn1ccc(NCCc2cnc[nH]2)nc1=O	10.1016/j.bmcl.2011.07.114
	CHEMBL1916493	67960	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	325	7	2	6	1.7	COc1ccccc1Cn1ccc(NCCc2cnc[nH]2)nc1=O	10.1016/j.bmcl.2011.07.114
	124087	1357	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	7157	1357	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	814	1357	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	CHEMBL1172	1357	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	DB00967	1357	106	None	-5888	15	Human	4.8	pKi	=	4.8	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	nan
	54587791	61199	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770983	61199	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	10515050	6157	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1081541	6157	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL350478	6157	0	None	-19	3	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.5	CCCC(C)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	73355143	91424	0	None	-22	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	323	8	2	4	2.8	O=C(NCCOc1ccccc1Cl)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL2413362	91424	0	None	-22	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	323	8	2	4	2.8	O=C(NCCOc1ccccc1Cl)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	56678756	65283	3	None	-	1	Human	5.8	pKi	=	5.8	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4	ChEMBL	391	5	1	4	4.2	CCC(=O)N[C@@H]1CC[C@H](C(=O)N(C)c2ccc(-c3nc4ccccc4o3)cc2)C1	nan
	CHEMBL1834199	65283	3	None	-	1	Human	5.8	pKi	=	5.8	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000738a HRH4	ChEMBL	391	5	1	4	4.2	CCC(=O)N[C@@H]1CC[C@H](C(=O)N(C)c2ccc(-c3nc4ccccc4o3)cc2)C1	nan
	137634349	155462	0	None	-741	2	Human	4.8	pKi	=	4.8	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	6	0	3	4.7	CC(=O)c1ccc(OCc2ccc(CN3CCCC(C)C3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4062122	155462	0	None	-741	2	Human	4.8	pKi	=	4.8	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	6	0	3	4.7	CC(=O)c1ccc(OCc2ccc(CN3CCCC(C)C3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	164626370	185881	0	None	-14	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	6	4	4	1.8	CCCCCCNC(=O)/N=C(\N)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	CHEMBL4876799	185881	0	None	-14	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	6	4	4	1.8	CCCCCCNC(=O)/N=C(\N)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	155568977	175568	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	281	6	3	3	2.6	c1ccc2[nH]c(CNCC[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	CHEMBL4592695	175568	0	None	-3	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	281	6	3	3	2.6	c1ccc2[nH]c(CNCC[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	24854706	95519	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)c(Cl)c2)CC1	10.1021/jm7014217
	CHEMBL259536	95519	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	388	3	0	5	4.5	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)c(Cl)c2)CC1	10.1021/jm7014217
	1278	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1279	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	4908365	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	CHEMBL129198	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	46929554	16560	0	None	11	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	349	5	1	3	4.7	FC(F)(F)c1ccc(SCC(c2ccncc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243367	16560	0	None	11	3	Human	7.8	pKi	=	7.8	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	349	5	1	3	4.7	FC(F)(F)c1ccc(SCC(c2ccncc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	4060730	19168	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129199	19168	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	49841608	69373	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935565	69373	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	321	1	1	5	1.7	CN1CCN(c2nc(N)nc3cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	1278	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	1279	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	4908365	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	CHEMBL129198	2110	79	None	-3	8	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm7014777
	4060730	19168	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129199	19168	51	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0502081
	45379805	197737	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	7	1.9	CN1CCN(c2nc(NCCS(=O)(=O)N3CCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591248	197737	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	7	1.9	CN1CCN(c2nc(NCCS(=O)(=O)N3CCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	164585473	183969	0	None	-3	5	Human	5.8	pKi	=	5.8	Binding	Binding affinity to H4 receptor (unknown origin)Binding affinity to H4 receptor (unknown origin)	ChEMBL	503	5	1	2	7.2	O=CN1CCC(c2ccc(-c3cc(C(=O)O)cc4cc(-c5ccc(C(F)(F)F)cc5)ccc34)cc2)CC1	10.1021/acs.jmedchem.1c00164
	CHEMBL4848380	183969	0	None	-3	5	Human	5.8	pKi	=	5.8	Binding	Binding affinity to H4 receptor (unknown origin)Binding affinity to H4 receptor (unknown origin)	ChEMBL	503	5	1	2	7.2	O=CN1CCC(c2ccc(-c3cc(C(=O)O)cc4cc(-c5ccc(C(F)(F)F)cc5)ccc34)cc2)CC1	10.1021/acs.jmedchem.1c00164
	71519177	86043	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314760	86043	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	112936262	136739	1	None	3	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2nc(-c3ccccc3)cc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752195	136739	1	None	3	2	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2nc(-c3ccccc3)cc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	1403136	179917	7	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL475331	179917	7	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm901379s
	164611143	184123	0	None	-75	4	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	414	6	4	4	2.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(F)(F)F	10.1016/j.ejmech.2021.113190
	CHEMBL4850448	184123	0	None	-75	4	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	414	6	4	4	2.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(F)(F)F	10.1016/j.ejmech.2021.113190
	137646744	157266	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	391	10	1	5	3.0	O=C(CCNC(=O)C(Cc1ccccc1)Cn1ccnc1)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4083336	157266	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	391	10	1	5	3.0	O=C(CCNC(=O)C(Cc1ccccc1)Cn1ccnc1)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	56835171	69382	0	None	-10	9	Rat	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from rat H4RDisplacement of [3H]histamine from rat H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69382	0	None	-10	9	Rat	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from rat H4RDisplacement of [3H]histamine from rat H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	90645149	111669	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1cccc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290574	111669	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1cccc(-c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2014.06.032
	46890021	7273	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.8	Cc1cc(OCCCNCC2CCCCN2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086328	7273	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.8	Cc1cc(OCCCNCC2CCCCN2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	118725541	116599	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	1	4	2.0	CCCCNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393528	116599	2	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	1	4	2.0	CCCCNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	16082858	79667	0	None	-8	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL213133	79667	0	None	-8	2	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	45379809	197698	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	420	5	1	8	2.1	CN1CCN(c2nc(NCCN3C(=O)CSC3=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591027	197698	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	420	5	1	8	2.1	CN1CCN(c2nc(NCCN3C(=O)CSC3=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	135398737	942	89	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	38	942	89	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	722	942	89	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	CHEMBL42	942	89	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	DB00363	942	89	None	-57	92	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm051008s
	10575070	67524	0	None	-79	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	439	7	1	3	4.7	O=C(c1cc2ccccc2[nH]1)N1CCN(CCOC(c2ccccc2)c2ccccc2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910383	67524	0	None	-79	2	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	439	7	1	3	4.7	O=C(c1cc2ccccc2[nH]1)N1CCN(CCOC(c2ccccc2)c2ccccc2)CC1	10.1016/j.bmcl.2011.09.001
	164622330	185270	0	None	-724	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4867962	185270	0	None	-724	6	Human	4.8	pKi	=	4.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	6	4	4	2.3	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	1267	3732	43	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	3035905	3732	43	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	CHEMBL260374	3732	43	None	-33	28	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.6b00120
	122193098	123386	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628045	123386	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645162	111684	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	324	2	1	6	1.3	CN1CCN(c2nc(N)nc(-c3cc(F)c(F)c(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290589	111684	0	None	-	1	Human	5.8	pKi	=	5.8	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	324	2	1	6	1.3	CN1CCN(c2nc(N)nc(-c3cc(F)c(F)c(F)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	24745507	67863	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915538	67863	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	302	5	3	6	1.2	CNC1CN(c2cc(NCc3cccc(F)c3)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	45257133	198212	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594721	198212	0	None	19	2	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccccc3Cl)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	24826165	67757	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914763	67757	0	None	-	1	Human	7.8	pKi	=	7.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	1	1	6	2.8	CN1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	1278	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	1279	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	4908365	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	CHEMBL129198	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.09.001
	3032915	15306	20	None	-1	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1021/jm0341047
	CHEMBL12160	15306	20	None	-1	7	Human	6.8	pKi	=	6.8	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1021/jm0341047
	127052294	139759	0	None	5	4	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139759	0	None	5	4	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	57394550	67759	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	2	1	7	2.2	COc1ccc2c(c1)sc1c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914765	67759	0	None	-	1	Human	6.8	pKi	=	6.8	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	2	1	7	2.2	COc1ccc2c(c1)sc1c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.08.014
	51354009	58560	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cc2cn(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688940	58560	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cc2cn(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	156011119	176538	0	None	-3	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	841	18	4	6	8.7	Cc1cc(OCCCNC(=O)CCCCCNC(=O)COc2ccc(/C=C/C3=[N+]4C(=Cc5ccc(-c6ccc[nH]6)n5[B-]4(F)F)C=C3)cc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4633688	176538	0	None	-3	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	841	18	4	6	8.7	Cc1cc(OCCCNC(=O)CCCCCNC(=O)COc2ccc(/C=C/C3=[N+]4C(=Cc5ccc(-c6ccc[nH]6)n5[B-]4(F)F)C=C3)cc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	51354296	58562	0	None	-4	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	274	6	1	5	1.6	c1nc(CCCn2cc(CN3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688942	58562	0	None	-4	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	274	6	1	5	1.6	c1nc(CCCn2cc(CN3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	1236	2028	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	1237	2028	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	156615	2028	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	CHEMBL268229	2028	23	None	-204	7	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1021/jm7014777
	756701	198125	29	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	218	1	1	5	0.1	CN1CCN(c2ncnc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594152	198125	29	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	218	1	1	5	0.1	CN1CCN(c2ncnc3[nH]cnc23)CC1	10.1016/j.bmc.2009.08.059
	63868328	173669	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4549015	173669	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	3	1	6	0.2	CCCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	58227381	176032	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4513989	176032	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597817	176032	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.3	CC(C)(C)CNc1nccc(N2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	25128822	186888	0	None	-10	5	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL494093	186888	0	None	-10	5	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	25179124	173651	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm800876b
	CHEMBL454883	173651	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm800876b
	25179122	192660	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL523941	192660	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179124	173651	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm901379s
	CHEMBL454883	173651	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	371	4	1	6	3.5	Cc1cc(Cl)cc2c(NCc3ccco3)nc(N3CCN(C)CC3)nc12	10.1021/jm901379s
	25179122	192660	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL523941	192660	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	403	4	1	5	3.9	CN1CCN(c2nc(NCc3ccc(F)c(F)c3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	1265	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	6422124	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	CHEMBL14638	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1021/jm8003625
	24994634	2102	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2102	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2102	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from rat histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2102	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2102	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2102	26	None	-21	7	Rat	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from rat recombinant histamine H4 receptorDisplacement of [3H]histamine from rat recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL4520788	212215	6	None	-89	25	Human	5.7	pKi	=	5.7	Binding	Selectivity interaction (GPCR panel (PDSP screen)) EUB0000743a HRH4Selectivity interaction (GPCR panel (PDSP screen)) EUB0000743a HRH4	ChEMBL		None	None	None		CNCc1ccccc1-c1csc([C@H](C)Nc2nc(C)nc3cc(OC)c(OC)cc23)c1	nan
	54581937	61179	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	3	1	5	1.3	CC(C)N(C)c1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770963	61179	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	3	1	5	1.3	CC(C)N(C)c1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	137646939	157184	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	335	5	3	4	3.6	CC(C)(CN=C(c1ccccc1O)c1ccccc1O)c1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	CHEMBL4082297	157184	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	335	5	3	4	3.6	CC(C)(CN=C(c1ccccc1O)c1ccccc1O)c1c[nH]cn1	10.1016/j.ejmech.2016.09.074
	9878733	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL338051	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9878733	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL338051	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0341047
	49841610	69374	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3cc(-c4ccccc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935566	69374	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3cc(-c4ccccc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	44547127	115405	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	44547127	115405	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354816	115405	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354816	115405	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	234	4	2	4	1.7	CN[C@@H]1CCN(c2ccnc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	24739219	67696	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	289	1	2	6	1.8	Nc1nc(N2CC(N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914546	67696	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	289	1	2	6	1.8	Nc1nc(N2CC(N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	24826265	67755	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.3	CN1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914761	67755	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.3	CN1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)CC1	10.1016/j.bmcl.2011.08.014
	9992865	113915	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL333220	113915	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	262	1	1	3	1.1	CN1CCN(C(=O)c2nc3cc(F)ccc3[nH]2)CC1	10.1021/jm0502081
	9878733	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338051	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1021/jm0502081
	2063135	8278	9	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL1093180	8278	9	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2010.02.097
	44129213	197692	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)c(Cl)cc3n2)CC1	10.1021/jm901379s
	CHEMBL590995	197692	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)c(Cl)cc3n2)CC1	10.1021/jm901379s
	71525681	89284	0	None	-2	6	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89284	0	None	-2	6	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	136141537	56172	1	None	2	3	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56172	1	None	2	3	Mouse	7.7	pKi	=	7.7	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	136030655	56173	0	None	-2	3	Rat	7.7	pKi	=	7.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56173	0	None	-2	3	Rat	7.7	pKi	=	7.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1278	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1279	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	4908365	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL129198	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2004.08.035
	1251	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	3035842	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL18661	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1251	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	3035842	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	CHEMBL18661	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	1251	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	3035842	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	CHEMBL18661	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm201042n
	11673377	138719	0	None	-1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	263	7	2	2	3.2	Clc1ccc(CNCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	CHEMBL379027	138719	0	None	-1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	263	7	2	2	3.2	Clc1ccc(CNCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	25129523	186694	40	None	1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	186694	40	None	1	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	66572114	75283	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047276	75283	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70690369	75323	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	1	2	2	2.0	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047473	75323	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	281	1	2	2	2.0	O=C(c1cc2cc(Cl)cc(F)c2[nH]1)N1CCNCC1	10.1016/j.ejmech.2012.06.016
	9878733	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL338051	116331	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3cccc(Cl)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1204	1893	114	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1247	1893	114	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1375	1893	114	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	774	1893	114	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	CHEMBL90	1893	114	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	DB05381	1893	114	None	-3	25	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1039/C2MD20212C
	1278	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2110	79	None	-3	8	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	11846298	138738	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	CHEMBL379102	138738	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccc(F)cc32)CC1	10.1021/jm051008s
	155538989	175745	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4513421	175745	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595438	175745	0	None	1	2	Human	5.7	pKi	=	5.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	3	5	2.1	CC(C)(C)CNc1nccc(NC2CCNCC2)n1	10.1021/acs.jmedchem.9b01342
	164608815	183862	0	None	-47	6	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	7	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4846638	183862	0	None	-47	6	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	7	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1016/j.ejmech.2021.113190
	164611342	184495	0	None	-416	6	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	346	6	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856005	184495	0	None	-416	6	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	346	6	4	4	1.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccccc1	10.1016/j.ejmech.2021.113190
	155513612	175736	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-2	4	Rat	5.7	pKi	=	5.7	Binding	Displacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from rat H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	156015838	177661	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4640263	177661	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650622	177661	0	None	-2	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	373	7	1	2	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	127036982	136929	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	525	6	1	6	5.9	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3c2CCc2ccccc2-3)cc1-c1ccc(Cl)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753784	136929	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	525	6	1	6	5.9	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3c2CCc2ccccc2-3)cc1-c1ccc(Cl)cc1	10.1016/j.bmcl.2015.12.035
	57393769	67813	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	281	1	2	6	1.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915030	67813	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	281	1	2	6	1.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	11688307	69545	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938968	69545	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	318	2	1	3	2.2	CCN1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	136141537	56172	1	None	-10	3	Rat	6.7	pKi	=	6.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56172	1	None	-10	3	Rat	6.7	pKi	=	6.7	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	44395364	66550	0	None	15	2	Human	6.7	pKi	=	6.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	276	2	1	2	3.3	CN1CCC(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186456	66550	0	None	15	2	Human	6.7	pKi	=	6.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	276	2	1	2	3.3	CN1CCC(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	10037650	5123	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106158	5123	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	135398737	942	89	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	38	942	89	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	722	942	89	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	CHEMBL42	942	89	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	DB00363	942	89	None	-57	92	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1021/jm201042n
	11572015	74665	0	None	-50	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	9	2	2	2.5	CCCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL203441	74665	0	None	-50	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	9	2	2	2.5	CCCCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11600780	75681	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	2	2	1.7	CC(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205732	75681	0	None	-2	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	2	2	1.7	CC(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	1408942	64777	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	254	2	0	4	1.9	CN1CCN(c2cc(-c3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824046	64777	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	254	2	0	4	1.9	CN1CCN(c2cc(-c3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.06.123
	4060730	19168	51	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19168	51	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	25129527	186821	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	186821	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	66573462	75303	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	2	2	2	2.2	CNC1CN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047453	75303	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	2	2	2	2.2	CNC1CN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	767246	95558	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	242	1	0	4	1.7	Cc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL259745	95558	4	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	242	1	0	4	1.7	Cc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	155550732	176045	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4540849	176045	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4597895	176045	0	None	-794	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	323	8	0	2	5.4	c1ccc(-c2ccc(OCCCCCN3CCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	11313837	2454	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	1254	2454	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	CHEMBL175782	2454	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmcl.2010.10.041
	11313837	2454	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	1254	2454	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	CHEMBL175782	2454	7	None	-1995	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptorDisplacement of [3H]histamine binding to SK-N-MC cell membranes expressing human histamine H4 receptor	ChEMBL	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049475h
	71681480	89217	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	307	6	3	7	2.0	Nc1nc(NCCNc2ccncn2)cc(-c2ccccc2)n1	10.1021/jm301886t
	CHEMBL2375761	89217	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	307	6	3	7	2.0	Nc1nc(NCCNc2ccncn2)cc(-c2ccccc2)n1	10.1021/jm301886t
	57392460	69508	0	None	14	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL1938843	69508	0	None	14	2	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572104	75269	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ccncc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047262	75269	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ccncc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572112	75281	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3ccc([N+](=O)[O-])cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047274	75281	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3ccc([N+](=O)[O-])cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	44395361	66591	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	293	1	2	3	2.0	CN1CCN(C(=O)Nc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186640	66591	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	293	1	2	3	2.0	CN1CCN(C(=O)Nc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	10879463	5203	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106570	5203	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	10945113	109128	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL322256	109128	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	127035836	136980	0	None	-5	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	4	1	5	3.9	Cc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3754176	136980	0	None	-5	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	4	1	5	3.9	Cc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	164618283	183951	0	None	-537	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	5	2.4	COc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848026	183951	0	None	-537	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	5	2.4	COc1ccc(C(C)NC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	53233805	61180	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770964	61180	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.5	CC(C)CNc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	54585781	61217	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1771000	61217	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	118720442	115408	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	3	5	1.4	Nc1cc(N2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354820	115408	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	3	5	1.4	Nc1cc(N2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	44547268	116597	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393526	116597	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	58227255	116610	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393539	116610	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2ccnc(NCC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	58227252	116615	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393544	116615	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	25112105	8064	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1091875	8064	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737949	67775	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914782	67775	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	2	2	6	1.9	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	24825996	67781	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914788	67781	0	None	-	1	Human	8.7	pKi	=	8.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	59777341	109646	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	275	3	2	5	1.9	CN[C@@H]1CCN(c2cc(C3CCCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236576	109646	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	275	3	2	5	1.9	CN[C@@H]1CCN(c2cc(C3CCCCC3)nc(N)n2)C1	10.1021/jm401727m
	1278	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	24745335	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	24745335	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	4	5	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	53257796	109645	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236575	109645	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	3	2	5	1.1	CN[C@@H]1CCN(c2cc(C3CCC3)nc(N)n2)C1	10.1021/jm401727m
	24738269	67693	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.1	Nc1nc(N2CC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914543	67693	0	None	-	1	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	1	2	6	2.1	Nc1nc(N2CC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	1223	938	32	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	938	32	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	938	32	None	-60	11	Human	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor T5.42A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	44231735	7688	0	None	-1	4	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089390	7688	0	None	-1	4	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	301	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC32CCCC2)C1	10.1016/j.bmcl.2010.01.131
	44462956	5508	0	None	2	2	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL1077276	5508	0	None	2	2	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	1278	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1278	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	1279	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	1279	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	4908365	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	4908365	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	CHEMBL129198	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8005959
	CHEMBL129198	2110	79	None	3	8	Rat	8.6	pKi	=	8.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800670r
	137657169	159028	0	None	3	2	Human	8.6	pKi	=	8.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	8	1	4	4.1	O=[N+]([O-])c1cccc(C(OCCCc2c[nH]cn2)c2ccccc2)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4102970	159028	0	None	3	2	Human	8.6	pKi	=	8.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	337	8	1	4	4.1	O=[N+]([O-])c1cccc(C(OCCCc2c[nH]cn2)c2ccccc2)c1	10.1016/j.ejmech.2016.09.074
	59777258	109631	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236561	109631	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.5	Nc1nc(C2CCCC2)cc(N2C[C@H]3CCN[C@H]3C2)n1	10.1021/jm401727m
	25130914	186661	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL492677	186661	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	46890547	6423	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082772	6423	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	7	2	4	4.0	CNC1CCN(CCCOc2ccc(-c3nc4c(C)c(F)ccc4[nH]3)c(C)c2)C1	10.1016/j.bmcl.2010.04.017
	10446295	2092	29	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	1277	2092	29	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	CHEMBL185951	2092	29	None	269	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm401727m
	68081305	109620	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	1	1	2	1.3	CN1CCN(C(=O)c2cc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	CHEMBL3236550	109620	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	1	1	2	1.3	CN1CCN(C(=O)c2cc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	24798144	109623	2	None	-19	3	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	CHEMBL3236553	109623	2	None	-19	3	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.1	Nc1nc(C2CCCC2)cc(N2CCNCC2)n1	10.1021/jm401727m
	24738103	67773	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(OC)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914780	67773	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	3	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(OC)c23)C1	10.1016/j.bmcl.2011.08.014
	24745335	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	-6	5	Mouse	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	16362	3064	67	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	2172	3064	67	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	90	3064	67	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	CHEMBL1423	3064	67	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	DB01100	3064	67	None	-562	30	Human	5.7	pKi	=	5.7	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	ChEMBL	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	10.1016/j.ejmech.2016.09.074
	9904236	13947	16	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL1197564	13947	16	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	295	0	1	3	3.7	O=C1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46890589	6929	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	7	1	4	4.8	Cc1cc(OCCCN2CCCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084809	6929	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	7	1	4	4.8	Cc1cc(OCCCN2CCCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57396382	67962	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	9	2	7	3.8	COCc1ccc(CN2CC[C@H](Nc3cc(NCCC(C)(C)C)ncn3)C2)o1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916495	67962	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	9	2	7	3.8	COCc1ccc(CN2CC[C@H](Nc3cc(NCCC(C)(C)C)ncn3)C2)o1	10.1016/j.bmcl.2011.07.114
	57391122	67963	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	7	3	6	3.9	CC(C)(C)CCNc1cc(N[C@H]2CCN(Cc3cccc(F)c3O)C2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916496	67963	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	387	7	3	6	3.9	CC(C)(C)CCNc1cc(N[C@H]2CCN(Cc3cccc(F)c3O)C2)ncn1	10.1016/j.bmcl.2011.07.114
	57394628	67968	1	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	4	2	5	1.7	CC(C)(C)CCNc1cc(N2CCNCC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916500	67968	1	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	4	2	5	1.7	CC(C)(C)CCNc1cc(N2CCNCC2)ncn1	10.1016/j.bmcl.2011.07.114
	57397738	69506	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	245	1	0	5	0.1	CN1CCN(C(=O)c2cn3ccncc3n2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938841	69506	0	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	245	1	0	5	0.1	CN1CCN(C(=O)c2cn3ccncc3n2)CC1	10.1016/j.bmcl.2011.11.098
	10399535	117924	3	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	2	2	1.6	CC1CN(C(=O)c2cc3ccccc3[nH]2)CCN1	10.1021/jm0341047
	CHEMBL341087	117924	3	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	243	1	2	2	1.6	CC1CN(C(=O)c2cc3ccccc3[nH]2)CCN1	10.1021/jm0341047
	90645147	111671	0	None	13	3	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290576	111671	0	None	13	3	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	155567108	176052	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4586599	176052	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597946	176052	0	None	3	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	223	5	3	5	1.3	CC(C)(C)CNc1nccc(NCCN)n1	10.1021/acs.jmedchem.9b01342
	155536509	175888	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4473753	175888	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596582	175888	0	None	-21	2	Human	6.7	pKi	=	6.7	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	274	6	3	5	2.3	CC(C)(C)CNc1nccc(NCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	4687476	95851	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL261320	95851	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	9928073	201945	0	None	-67	3	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	343	0	0	4	4.1	CN1CCN(C2=Nc3cc(Cl)ccc3Sc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL67284	201945	0	None	-67	3	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	343	0	0	4	4.1	CN1CCN(C2=Nc3cc(Cl)ccc3Sc3ccccc32)CC1	10.1021/jm051008s
	90654501	109807	0	None	-21877	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	314	6	0	5	3.1	Cc1nnc(C)n1-c1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	CHEMBL3238444	109807	0	None	-21877	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	314	6	0	5	3.1	Cc1nnc(C)n1-c1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	4687476	95851	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1021/jm7014217
	CHEMBL261320	95851	4	None	-	1	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2nccc3ccccc23)CC1	10.1021/jm7014217
	10879463	5203	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106570	5203	0	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	46228132	200450	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL609161	200450	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	46228071	198484	15	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL596562	198484	15	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	51354870	58581	0	None	-26	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	305	6	0	5	2.0	CN1CCN(CCCn2cc(CC3CCCCC3)nn2)CC1	10.1021/jm1013488
	CHEMBL1688981	58581	0	None	-26	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	305	6	0	5	2.0	CN1CCN(CCCn2cc(CC3CCCCC3)nn2)CC1	10.1021/jm1013488
	57391928	69551	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4ccc(Cl)nc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938973	69551	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4ccc(Cl)nc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	23650960	148585	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(F)ccc2[nH]1	nan
	CHEMBL3943309	148585	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(F)ccc2[nH]1	nan
	42624149	109638	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3236568	109638	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1016/j.bmcl.2014.10.013
	25218146	116592	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	2	0	4	2.6	CN1CCN(c2ccnc(-c3ccsc3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393521	116592	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	2	0	4	2.6	CN1CCN(c2ccnc(-c3ccsc3)c2)CC1	10.1016/j.bmcl.2014.12.027
	42624149	109638	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109638	1	None	3	3	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	53258370	109639	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236569	109639	1	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CCc1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	57394548	67741	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@@H]3CCCN[C@@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914747	67741	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@@H]3CCCN[C@@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	57396298	67746	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	1	1	6	1.8	CN1CCN(c2nc(N)nc3c2oc2cccc(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914752	67746	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	301	1	1	6	1.8	CN1CCN(c2nc(N)nc3c2oc2cccc(F)c23)CC1	10.1016/j.bmcl.2011.08.014
	44628629	67761	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	269	1	2	6	1.4	Nc1nc(N2CCNCC2)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914767	67761	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	269	1	2	6	1.4	Nc1nc(N2CCNCC2)c2oc3ccccc3c2n1	10.1016/j.bmcl.2011.08.014
	11630326	72211	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199231	72211	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	263	1	1	3	1.9	Cc1csc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	51354582	58575	0	None	2	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.3	CC(c1cn(CCCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	CHEMBL1688974	58575	0	None	2	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.3	CC(c1cn(CCCc2c[nH]cn2)nn1)C1CCCCC1	10.1021/jm1013488
	71603195	89279	0	None	8	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	3	2	5	1.9	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376798	89279	0	None	8	2	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	3	2	5	1.9	CN[C@@H]1CCN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	1251	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	3035842	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	CHEMBL18661	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1021/jm049932u
	1530	2142	44	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	3827	2142	44	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	7206	2142	44	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	CHEMBL534	2142	44	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	DB00920	2142	44	None	-21379	21	Human	4.7	pKi	=	4.7	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	nan
	57399107	67857	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1021/acs.jmedchem.5b00516
	CHEMBL1915532	67857	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1021/acs.jmedchem.5b00516
	57399107	67857	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915532	67857	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.125
	57399107	67857	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915532	67857	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	373	8	3	7	3.1	CC(C)(C)CCNc1cc(NC2CCN(Cc3ccc(CO)o3)C2)ncn1	10.1016/j.bmcl.2011.07.114
	57395619	67858	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915533	67858	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	57395619	67858	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915533	67858	13	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	309	3	1	5	1.8	CN1CCN(c2cc(NC3Cc4ccccc4C3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	168268680	192154	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5172675	192154	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221210	192154	0	None	-2	4	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	1403136	179917	7	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL475331	179917	7	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)c3ccccc3n2)CC1	10.1021/jm800876b
	1204	1893	114	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-15	25	Mouse	5.7	pKi	=	5.7	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	164610824	184517	0	None	-14	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	452	8	4	4	3.6	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856200	184517	0	None	-14	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	452	8	4	4	3.6	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	10945113	109128	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL322256	109128	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	21896704	158967	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	429	9	1	7	2.7	CC(=O)SCC(Cc1ccc2c(c1)OCO2)C(=O)NCC(=O)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4102243	158967	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	429	9	1	7	2.7	CC(=O)SCC(Cc1ccc2c(c1)OCO2)C(=O)NCC(=O)OCc1ccccc1	10.1016/j.ejmech.2016.09.074
	46912441	10786	0	None	-117	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	309	3	1	2	3.9	FC(F)(F)c1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1172754	10786	0	None	-117	2	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	309	3	1	2	3.9	FC(F)(F)c1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	118725538	116593	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	3	1	4	2.6	CN[C@@H]1CCN(c2ccnc(-c3ccsc3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393522	116593	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	259	3	1	4	2.6	CN[C@@H]1CCN(c2ccnc(-c3ccsc3)c2)C1	10.1016/j.bmcl.2014.12.027
	1204	1893	114	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	6.7	pKi	=	6.7	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	155567030	175833	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4586133	175833	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	CHEMBL4596108	175833	0	None	-645	4	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter methodDisplacement of [3H]histamine from human recombinant H4R expressed in CHO cells incubated for 60 mins by microbeta scintillation counter method	ChEMBL	337	8	0	2	5.8	c1ccc(-c2ccc(OCCCCCN3CCCCCC3)cc2)cc1	10.1016/j.bmc.2017.07.058
	24854708	155170	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL405416	155170	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	51354587	58580	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	0	5	2.7	c1cn(CCCn2cc(CC3CCCCC3)nn2)cn1	10.1021/jm1013488
	CHEMBL1688979	58580	0	None	-3	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	0	5	2.7	c1cn(CCCn2cc(CC3CCCCC3)nn2)cn1	10.1021/jm1013488
	10702196	108068	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL320279	108068	1	None	-1	2	Human	4.7	pKi	=	4.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	165	2	2	3	0.4	NC[C@@H]1C=C[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	136016345	10271	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	377	5	4	6	2.1	CCOC(=O)c1ccc(NC2=CC(=O)/C(=N\NC(=N)N)c3ccccc32)cc1	10.1021/jm7014777
	CHEMBL1162352	10271	2	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	377	5	4	6	2.1	CCOC(=O)c1ccc(NC2=CC(=O)/C(=N\NC(=N)N)c3ccccc32)cc1	10.1021/jm7014777
	127052293	139695	1	None	34	4	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139695	1	None	34	4	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1251	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	3035842	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	CHEMBL18661	1975	34	None	-100	5	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	10.1016/j.bmc.2010.04.052
	11258459	83800	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	CHEMBL220933	83800	0	None	-	1	Human	7.7	pKi	=	7.7	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1cc(C)c2nc(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)[nH]c2c1	10.1016/j.bmcl.2006.08.117
	46228082	200683	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	340	5	2	6	2.5	CC(C)c1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL610626	200683	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	340	5	2	6	2.5	CC(C)c1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	49841721	69366	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	CHEMBL1935558	69366	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	25178551	173649	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454882	173649	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm800876b
	25178551	173649	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454882	173649	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	341	4	1	6	2.7	CN1CCN(c2nc(NCc3ccco3)c3cc(F)ccc3n2)CC1	10.1021/jm901379s
	45379807	197818	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	6	3.5	CN1CCN(c2nc(NCCC(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591970	197818	0	None	-	1	Human	6.7	pKi	=	6.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	438	6	1	6	3.5	CN1CCN(c2nc(NCCC(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10037650	5123	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106158	5123	0	None	31	2	Human	6.7	pKi	=	6.7	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	122194624	123489	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccccc1/C=C/c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629827	123489	0	None	-	1	Human	5.7	pKi	=	5.7	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccccc1/C=C/c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2015.08.014
	57400700	67521	0	None	-3090	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	470	8	1	6	3.4	COc1ccc(CN(CCN2CCN(C(=O)c3nc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910380	67521	0	None	-3090	3	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	470	8	1	6	3.4	COc1ccc(CN(CCN2CCN(C(=O)c3nc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	164610421	184668	0	None	-7	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	332	5	4	4	2.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)Nc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4858567	184668	0	None	-7	2	Human	4.7	pKi	=	4.7	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	332	5	4	4	2.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)Nc1ccccc1	10.1016/j.ejmech.2021.113190
	46890120	6444	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	416	6	1	4	5.2	Cc1cc(OCCCN2Cc3cccnc3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082851	6444	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	416	6	1	4	5.2	Cc1cc(OCCCN2Cc3cccnc3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	56851604	71270	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923028	71270	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963460	71270	0	None	-13	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	8	2	3	5.1	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	24853663	95572	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	272	3	0	5	1.8	CCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL259839	95572	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	272	3	0	5	1.8	CCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	24278976	95791	56	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2nc3ccccc3nc2Cl)CC1	10.1021/jm7014217
	CHEMBL261010	95791	56	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2nc3ccccc3nc2Cl)CC1	10.1021/jm7014217
	24854704	95675	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	CHEMBL260456	95675	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	10923241	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128958	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10923241	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128958	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0341047
	56835171	69382	0	None	-1	9	Mouse	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from mouse H4RDisplacement of [3H]histamine from mouse H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935574	69382	0	None	-1	9	Mouse	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from mouse H4RDisplacement of [3H]histamine from mouse H4R	ChEMBL	295	2	2	6	1.9	Nc1nc(N2CCNCC2)c2ccc(-c3ccco3)cc2n1	10.1016/j.bmcl.2011.10.104
	11687917	14781	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	CHEMBL120854	14781	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	292	1	1	3	1.9	Cc1cc(Cl)c2nc(C(=O)N3CCN(C)CC3)[nH]c2c1	10.1021/jm0502081
	10923241	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128958	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1021/jm0502081
	45257131	198216	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198216	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	57403350	67965	0	None	35	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	3	1	5	1.7	CN1CCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916498	67965	0	None	35	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	3	1	5	1.7	CN1CCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	24804058	143553	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	424	7	2	5	5.1	Cc1c(F)ccc2[nH]c(-c3cnc(NCCCC4CCN(C)CC4)nc3C(C)C)nc12	nan
	CHEMBL3903520	143553	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	424	7	2	5	5.1	Cc1c(F)ccc2[nH]c(-c3cnc(NCCCC4CCN(C)CC4)nc3C(C)C)nc12	nan
	126963284	144229	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	CHEMBL3909134	144229	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.Binding Assay: The specific binding of the radioactive ligand to the receptor was defined as the difference between total binding and nonspecific binding, determined in the presence of excess unlabeled ligand. [3H]-histamine was used as the ligand in this study and the affinity values were determined according to the Cheng-Prusoff equation.	ChEMBL	311	1	1	3	4.2	CO[C@H]1Cc2ccccc2C(=C2CCNCC2)c2ccsc21	nan
	46890675	7147	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	1	4	4.4	Cc1cc(OCCCN2CC3CCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085803	7147	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	1	4	4.4	Cc1cc(OCCCN2CC3CCN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	49841722	69367	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3c(-c4ccc(C#N)cc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935559	69367	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3c(-c4ccc(C#N)cc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	168276867	192223	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174278	192223	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221653	192223	0	None	-4	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	662	11	8	8	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCCCCCCNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)c1	10.1021/acs.jmedchem.1c00692
	24853598	95763	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	350	4	0	6	3.2	COc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL260851	95763	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	350	4	0	6	3.2	COc1ccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	24853596	95838	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	354	3	0	5	3.8	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	CHEMBL261225	95838	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	354	3	0	5	3.8	CN1CCN(c2nc3ccccc3nc2Oc2ccc(Cl)cc2)CC1	10.1021/jm7014217
	164610289	184540	0	None	-38	4	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	7	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4856602	184540	0	None	-38	4	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	7	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	57399827	67969	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916501	67969	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2cc(NCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	90645155	111677	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290582	111677	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	90645155	111677	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL3290582	111677	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3ccc(C#N)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	46228114	198482	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596551	198482	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	314	4	3	7	1.1	CN1CCN(c2cc(NCc3ccc(O)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	25178131	186345	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	381	5	1	5	3.7	CN1CCN(c2nc(NCCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL490072	186345	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	381	5	1	5	3.7	CN1CCN(c2nc(NCCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	90645152	111674	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	396	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(I)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290579	111674	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	396	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3ccc(I)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	49818718	69372	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935564	69372	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	54582867	61215	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770999	61215	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	25147771	173656	0	None	1	3	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	173656	0	None	1	3	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	11440060	205293	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	368	8	1	2	5.4	c1ccc(C(OCCCc2c[nH]cn2)(c2ccccc2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL91576	205293	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	368	8	1	2	5.4	c1ccc(C(OCCCc2c[nH]cn2)(c2ccccc2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	127051301	139657	0	None	5	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139657	0	None	5	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	58227216	116625	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393554	116625	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	550981	68674	12	None	2	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	280	5	2	3	2.9	OC(CNC1CC1)Cn1c2ccccc2c2ccccc21	10.1021/jm2011589
	CHEMBL1923533	68674	12	None	2	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	280	5	2	3	2.9	OC(CNC1CC1)Cn1c2ccccc2c2ccccc21	10.1021/jm2011589
	24804056	150107	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	420	8	2	5	5.1	CCCc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(C)ccc2[nH]1	nan
	CHEMBL3955597	150107	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	420	8	2	5	5.1	CCCc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)c(C)ccc2[nH]1	nan
	71461658	78632	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	4	0.5	CN1CCN(C(=O)c2nc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL2113162	78632	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	259	1	2	4	0.5	CN1CCN(C(=O)c2nc3cc(N)ccc3[nH]2)CC1	10.1021/jm0502081
	4024	1976	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	9989505	1976	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	CHEMBL82298	1976	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1021/jm049932u
	122193101	123389	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.4	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C(F)(F)F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628048	123389	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.4	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C(F)(F)F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	155546701	173073	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	2	1	6	0.8	CN1CCN(c2nc(N)nc(CC(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4534504	173073	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	264	2	1	6	0.8	CN1CCN(c2nc(N)nc(CC(C)(C)C)n2)CC1	10.1016/j.bmc.2019.02.020
	24798596	198175	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594491	198175	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	310	2	1	6	1.3	CN1CCN(c2cc(N3Cc4ccccc4C3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	21193534	11145	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL1179089	11145	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL59679	11145	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	292	7	1	2	4.1	c1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	54587789	61192	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(NCCO)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770976	61192	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	4	3	7	-0.8	CN1CCN(c2cc(NCCO)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	45257131	198216	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	CHEMBL594731	198216	0	None	18	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1ccccc1CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmc.2009.08.059
	90655027	109625	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236555	109625	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	3	2	5	1.5	CN[C@H]1CCN(c2cc(C3CCCC3)nc(N)n2)C1	10.1021/jm401727m
	66740898	109632	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	CHEMBL3236562	109632	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	287	2	1	5	1.7	CN1C[C@H]2CN(c3cc(C4CCCC4)nc(N)n3)C[C@H]2C1	10.1021/jm401727m
	24826082	67784	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914791	67784	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)C1	10.1016/j.bmcl.2011.08.014
	11587279	124518	3	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL364400	124518	3	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3cc(Cl)sc3[nH]2)CC1	10.1021/jm0502081
	1226	1980	16	None	2	7	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	41376	1980	16	None	2	7	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	CHEMBL12608	1980	16	None	2	7	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	10.1016/j.bmcl.2010.10.041
	10105117	103705	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL309819	103705	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	226	6	3	2	1.6	CC/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10466840	202766	0	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CC/N=C(\S)NCCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL72424	202766	0	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	7	3	2	2.0	CC/N=C(\S)NCCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1247	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	1375	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	774	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	CHEMBL90	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	DB05381	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.1c00692
	71681476	89212	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	316	8	4	8	1.5	CC(C)CCc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375756	89212	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	316	8	4	8	1.5	CC(C)CCc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	4060730	19168	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm301886t
	CHEMBL129199	19168	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm301886t
	10923241	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL128958	18957	1	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3cc(O)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	4060730	19168	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129199	19168	51	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	22660616	75280	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3ccc(O)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047273	75280	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	259	1	2	3	1.3	CN1CCN(C(=O)c2cc3ccc(O)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	71525588	89286	0	None	33	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.1	CNC1CN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376805	89286	0	None	33	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.1	CNC1CN(c2nc(N)nc3cc(C(C)C)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846300	79841	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm7014217
	CHEMBL213912	79841	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm7014217
	45271470	193370	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL1202329	193370	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	CHEMBL549481	193370	0	None	-3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	6	3	3	3.1	Cc1ccc(CNC(=N)SCCCc2c[nH]cn2)cc1	10.1016/j.bmc.2009.04.007
	45270644	193791	0	None	3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	257	6	4	2	1.7	N=C(NCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmc.2009.04.007
	CHEMBL552694	193791	0	None	3	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	257	6	4	2	1.7	N=C(NCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmc.2009.04.007
	45271464	193821	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202328	193821	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL553455	193821	0	None	-5	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	292	6	3	3	2.9	N=C(NCc1ccc(F)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10468601	193908	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202324	193908	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL555434	193908	0	None	-2	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	274	6	3	3	2.8	N=C(NCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	45268095	194356	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202323	194356	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560313	194356	0	None	1	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	378	9	3	3	4.8	N=C(NCCC(c1ccccc1)c1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1204	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1247	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1375	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	774	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	CHEMBL90	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	DB05381	1893	114	None	-3	25	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.ejmech.2021.113190
	1274	3989	12	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	3063228	3989	12	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	CHEMBL1196470	3989	12	None	19	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1039/C2MD20212C
	10988959	117592	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL340740	117592	4	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1039/C2MD20212C
	21638785	188063	4	None	-4	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188063	4	None	-4	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	127052294	139759	0	None	5	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806079	139759	0	None	5	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	11751419	137342	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL376268	137342	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	11751419	137342	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2006.08.117
	CHEMBL376268	137342	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	7	1	4	5.6	CN(C)C1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2006.08.117
	57402512	67810	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915022	67810	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	3	2	7	2.2	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(OC)ccc23)C1	10.1016/j.bmcl.2011.08.014
	46228092	198256	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	299	4	1	6	1.4	CN1CCN(c2cc(OCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594973	198256	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	299	4	1	6	1.4	CN1CCN(c2cc(OCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	4024	1976	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	9989505	1976	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL82298	1976	14	None	-301	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	159	2	1	2	1.4	n1ccc(cc1)Cc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	1252	1978	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	9793868	1978	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL417096	1978	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1236	2028	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1237	2028	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	156615	2028	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL268229	2028	23	None	-204	7	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	1252	1978	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	9793868	1978	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	CHEMBL417096	1978	16	None	-100	4	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/jm0504353
	81371	12646	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187946	12646	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	CHEMBL534494	12646	11	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	178	3	4	4	0.2	N=C(N)SCCSC(=N)N	10.1021/jm060880d
	52128564	75266	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	0	3	2.5	CN1CCN(C(=O)c2cc3cc(Cl)ccc3o2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047259	75266	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	278	1	0	3	2.5	CN1CCN(C(=O)c2cc3cc(Cl)ccc3o2)CC1	10.1016/j.ejmech.2012.06.016
	70684084	75302	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	2	2	2.6	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047452	75302	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	2	2	2.6	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	70692528	75308	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047458	75308	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70686224	75322	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	2	2.4	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047472	75322	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	2	2	2	2.4	CN[C@@H]1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	51355156	58587	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	3.1	c1nc(CCCc2cn(C3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688989	58587	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	5	1	4	3.1	c1nc(CCCc2cn(C3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	9990475	56168	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	CHEMBL1632408	56168	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	CHEMBL2448450	56168	0	None	3	3	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	205	2	2	3	-0.5	Cc1nc(N)[se]c1CCN	10.1016/j.bmcl.2010.10.041
	10583206	108859	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL321920	108859	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	71681640	89219	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nc(Cl)cc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375763	89219	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nc(Cl)cc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	12438303	75265	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	260	1	0	3	2.3	CN1CCN(C(=O)c2cc3ccccc3s2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047258	75265	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	260	1	0	3	2.3	CN1CCN(C(=O)c2cc3ccccc3s2)CC1	10.1016/j.ejmech.2012.06.016
	22660598	75290	1	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047440	75290	1	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	273	2	1	3	1.6	COc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24854636	155764	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	296	1	0	4	2.4	CN1CCN(c2nc3ccccc3nc2C(F)(F)F)CC1	10.1021/jm7014217
	CHEMBL406558	155764	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	296	1	0	4	2.4	CN1CCN(c2nc3ccccc3nc2C(F)(F)F)CC1	10.1021/jm7014217
	10583206	108859	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL321920	108859	0	None	-1	2	Human	5.6	pKi	=	5.6	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	44395263	66355	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	292	2	2	3	1.7	CN1CCN(NC(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL185546	66355	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	292	2	2	3	1.7	CN1CCN(NC(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	44395336	66442	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	280	4	1	3	1.9	CN(C)CCN(C)C(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL185948	66442	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	280	4	1	3	1.9	CN(C)CCN(C)C(=O)c1nc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	44395290	124984	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	279	4	1	2	2.5	CN(C)CCN(C)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL364629	124984	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	279	4	1	2	2.5	CN(C)CCN(C)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	15984057	139578	0	None	-35481	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	348	5	0	2	5.3	c1cc(-c2ccc(CN3CCCCC3)cc2)ccc1CN1CCCCC1	10.1016/j.bmc.2008.10.029
	CHEMBL380101	139578	0	None	-35481	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	348	5	0	2	5.3	c1cc(-c2ccc(CN3CCCCC3)cc2)ccc1CN1CCCCC1	10.1016/j.bmc.2008.10.029
	71519348	86049	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	3	2	6	1.5	Nc1nc(N2CCNCC2)c2ccn(Cc3ccccc3)c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314766	86049	0	None	-	1	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	3	2	6	1.5	Nc1nc(N2CCNCC2)c2ccn(Cc3ccccc3)c2n1	10.1016/j.bmcl.2012.10.139
	122193106	123393	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	335	3	2	6	1.9	CN1CCN(c2nc(N)nc(/C=C/c3c[nH]c4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628052	123393	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	335	3	2	6	1.9	CN1CCN(c2nc(N)nc(/C=C/c3c[nH]c4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	122193095	123383	0	None	3	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123383	0	None	3	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	54585776	61177	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(N)nc(NCCO)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770961	61177	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(N)nc(NCCO)n2)C1	10.1016/j.bmcl.2011.03.017
	24994630	109629	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	2	5	1.6	Nc1nc(C2CCCC2)cc(N2CCC[C@@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236559	109629	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	261	2	2	5	1.6	Nc1nc(C2CCCC2)cc(N2CCC[C@@H](N)C2)n1	10.1021/jm401727m
	25178933	186344	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL490071	186344	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178933	186344	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL490071	186344	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	367	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	122193103	123391	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	341	4	1	8	1.3	CN1CCN(c2nc(N)nc(/C=C/c3cccc([N+](=O)[O-])c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628050	123391	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	341	4	1	8	1.3	CN1CCN(c2nc(N)nc(/C=C/c3cccc([N+](=O)[O-])c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	10446295	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	1277	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL185951	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.10.104
	10446295	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	1277	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	CHEMBL185951	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.03.017
	46890627	6496	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083098	6496	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	2	4	4.0	Cc1cc(OCCCN2CC3CCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11340020	83833	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	CHEMBL221210	83833	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	136042265	69552	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938974	69552	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	303	1	2	3	2.0	CN1C[C@H]2CN(C(=N)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11340020	83833	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL221210	83833	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	454	6	1	4	5.6	CN1CCCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	11189343	141433	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	420	6	1	4	4.9	Cc1cc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)ccc1OCCCN1CCN(C)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL387342	141433	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	420	6	1	4	4.9	Cc1cc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)ccc1OCCCN1CCN(C)CC1	10.1016/j.bmcl.2006.08.117
	57394549	67742	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914748	67742	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	10446295	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	1277	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	CHEMBL185951	2092	29	None	269	2	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1021/jm0502081
	24759624	95673	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	0	5	2.0	COc1nc2cc(Cl)ccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL260444	95673	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	0	5	2.0	COc1nc2cc(Cl)ccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	25147771	173656	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL454890	173656	0	None	-1	3	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	44129211	199280	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	383	5	1	7	1.5	CN1CCN(c2nc(NCCS(C)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL601946	199280	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	383	5	1	7	1.5	CN1CCN(c2nc(NCCS(C)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11821243	18649	8	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	2	1	2	1.9	CCN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128134	18649	8	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	2	1	2	1.9	CCN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1021/jm0341047
	155549319	175740	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4538671	175740	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595399	175740	0	None	1	2	Human	5.6	pKi	=	5.6	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	5	2	5	2.0	CCC(=O)N[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	164620704	185491	0	None	-17	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	8	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)c1ccc(C(C)C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4871482	185491	0	None	-17	4	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	8	4	4	3.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)c1ccc(C(C)C)cc1	10.1016/j.ejmech.2021.113190
	127026016	136951	0	None	-13	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753967	136951	0	None	-13	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	385	4	1	5	3.6	CN1CCN(c2nc(NCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	71518861	86056	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	5	2.1	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314774	86056	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	5	2.1	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	122193102	123390	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	321	3	1	7	1.2	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628049	123390	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	321	3	1	7	1.2	CN1CCN(c2nc(N)nc(/C=C/c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	63868018	174794	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	208	1	1	6	-0.5	Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4574940	174794	2	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	208	1	1	6	-0.5	Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71518536	86053	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	3	5	0.3	Cc1cc2c(N3CCNCC3)nc(N)nc2[nH]1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314771	86053	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	3	5	0.3	Cc1cc2c(N3CCNCC3)nc(N)nc2[nH]1	10.1016/j.bmcl.2012.10.139
	57391927	69546	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938969	69546	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	332	2	1	3	2.6	CC(C)N1C[C@H]2CN(C(=O)c3nc4cc(Cl)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	3991311	136325	9	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	296	2	0	5	2.7	CCc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	CHEMBL3741986	136325	9	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	296	2	0	5	2.7	CCc1nc(N2CCN(C)CC2)c2oc3ccccc3c2n1	10.1039/C5MD00022J
	25178556	188486	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL509961	188486	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	44563707	200902	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccc(Cl)cc3nc2OCc2ccccc2)CC1	10.1021/jm800876b
	CHEMBL612059	200902	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccc(Cl)cc3nc2OCc2ccccc2)CC1	10.1021/jm800876b
	24853595	159612	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2cccc(Cl)c2)CC1	10.1021/jm7014217
	CHEMBL410925	159612	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	368	4	0	5	3.6	CN1CCN(c2nc3ccccc3nc2OCc2cccc(Cl)c2)CC1	10.1021/jm7014217
	155535098	175802	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4471442	175802	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595908	175802	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	305	3	3	5	1.3	CC(C)(C)CNc1nccc(N2CCCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	10782821	95762	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	332	4	0	4	3.4	CCN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260850	95762	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	332	4	0	4	3.4	CCN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	164616851	184357	0	None	-446	5	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4853772	184357	0	None	-446	5	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	10591811	158794	0	None	4	2	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	9	1	2	4.7	c1ccc(-c2ccc(CCCOCCCc3c[nH]cn3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4100315	158794	0	None	4	2	Human	7.6	pKi	=	7.6	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	320	9	1	2	4.7	c1ccc(-c2ccc(CCCOCCCc3c[nH]cn3)cc2)cc1	10.1016/j.ejmech.2016.09.074
	1267	3732	43	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	3035905	3732	43	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	CHEMBL260374	3732	43	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Binding affinity to the human histamine H4 receptorBinding affinity to the human histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm0341047
	24804219	148019	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	378	6	2	5	4.2	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cccc2[nH]1	nan
	CHEMBL3938862	148019	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	378	6	2	5	4.2	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cccc2[nH]1	nan
	11565330	96758	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	CHEMBL268525	96758	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	275	1	1	2	2.0	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1021/jm0502081
	1267	3732	43	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	3035905	3732	43	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	CHEMBL260374	3732	43	None	-33	28	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2010.02.097
	71525681	89284	0	None	-3	6	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89284	0	None	-3	6	Rat	7.6	pKi	=	7.6	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	11846634	77640	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209758	77640	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	57395496	67816	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915034	67816	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	51354302	58571	0	None	1	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688970	58571	0	None	1	2	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25130235	186994	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	186994	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	186994	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	186994	1	None	-7	11	Rat	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	57390225	67525	0	None	-3235	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	9	2	5	4.1	COc1ccc(CN(CCN[C@H]2CCN(C(=O)c3cc4ccccc4[nH]3)C2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910384	67525	0	None	-3235	3	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	9	2	5	4.1	COc1ccc(CN(CCN[C@H]2CCN(C(=O)c3cc4ccccc4[nH]3)C2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	55251397	172683	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	2	1	6	0.3	CC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4525008	172683	2	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	236	2	1	6	0.3	CC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	53233803	61178	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770962	61178	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	3	2	5	1.2	CC(C)Nc1cc(N2CCN(C)CC2)cc(N)n1	10.1016/j.bmcl.2011.03.017
	11293460	84405	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	434	6	1	4	5.1	CN1CCN(CCCOc2ccc(-c3nc4c(ccc5ccccc54)[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL223490	84405	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	434	6	1	4	5.1	CN1CCN(CCCOc2ccc(-c3nc4c(ccc5ccccc54)[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	42624148	109644	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236574	109644	0	None	-	1	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	3	2	5	0.7	CN[C@@H]1CCN(c2cc(C3CC3)nc(N)n2)C1	10.1021/jm401727m
	11846300	79841	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm800876b
	CHEMBL213912	79841	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm800876b
	11846300	79841	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL213912	79841	3	None	-2	4	Human	7.6	pKi	=	7.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1021/jm051008s
	217674	178691	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL473098	178691	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	217674	178691	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL473098	178691	6	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc(Oc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	156009715	177735	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634631	177735	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651103	177735	0	None	-1	2	Human	4.6	pKi	=	4.6	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	601	8	1	3	8.2	Cc1cc(OCCC[n+]2c(C)cc(/C=C/c3cc4c5c(c3)CCCN5CCC4)cc2C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	45257134	198251	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	CHEMBL594949	198251	0	None	-	1	Human	6.6	pKi	=	6.6	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	312	4	2	6	1.7	Cc1cccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)c1	10.1016/j.bmc.2009.08.059
	155539562	172264	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	304	4	1	6	1.7	CN1CCN(c2nc(N)nc(CCC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	CHEMBL4514667	172264	0	None	-	1	Human	5.6	pKi	=	5.6	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	304	4	1	6	1.7	CN1CCN(c2nc(N)nc(CCC3CCCCC3)n2)CC1	10.1016/j.bmc.2019.02.020
	10083188	140390	3	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	CHEMBL382276	140390	3	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2011.09.001
	9927983	79427	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL1795042	79427	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL212212	79427	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	341	1	0	4	4.2	CCN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	49841612	69376	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3cc(-c4ccc(C#N)cc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935568	69376	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	344	2	1	6	2.5	CN1CCN(c2nc(N)nc3cc(-c4ccc(C#N)cc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	54587786	61186	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770970	61186	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	263	4	2	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(N(C)C(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	24825897	67753	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	1	1	6	2.2	CN1CCN(c2nc(N)nc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914759	67753	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	1	1	6	2.2	CN1CCN(c2nc(N)nc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	71519674	89285	0	None	-3	5	Rat	7.5	pKi	=	7.5	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89285	0	None	-3	5	Rat	7.5	pKi	=	7.5	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	56851610	71268	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923031	71268	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963448	71268	0	None	2	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	5	2	3	3.8	CC(C)(C)N/C(=N/C1CCCC1)SCCCc1c[nH]cn1	10.1021/jm201042n
	127049856	139702	0	None	2	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805431	139702	0	None	2	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	10680721	5889	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1080089	5889	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL158085	5889	0	None	-23	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	225	6	2	3	1.9	CC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	753256	178760	10	None	2	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL473662	178760	10	None	2	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228133	198361	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595639	198361	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3cccc(Cl)c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	29136870	68664	7	None	-46	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	301	2	0	7	0.7	CN1CCN(c2nc(N3CCCC3)c3cnn(C)c3n2)CC1	10.1021/jm2011589
	CHEMBL1923522	68664	7	None	-46	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	301	2	0	7	0.7	CN1CCN(c2nc(N3CCCC3)c3cnn(C)c3n2)CC1	10.1021/jm2011589
	71519176	86042	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	2	5	2.5	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314759	86042	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	2	5	2.5	CN1CCCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	155545717	172887	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	238	3	2	2	2.6	c1ccc2[nH]c(C[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	CHEMBL4529871	172887	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 30 mins by liquid scintillation analysis	ChEMBL	238	3	2	2	2.6	c1ccc2[nH]c(C[C@@H]3C[C@H]3c3c[nH]cn3)nc2c1	10.1016/j.bmcl.2018.10.041
	156015791	177659	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4646286	177659	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650609	177659	0	None	1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	442	8	1	3	4.6	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCN1CCc2nc[nH]c2C1	10.1021/acs.jmedchem.0c00160
	24854638	154644	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL402470	154644	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCc2ccccc2)CC1	10.1021/jm7014217
	24854702	95598	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2cccnc2)CC1	10.1021/jm7014217
	CHEMBL259955	95598	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2cccnc2)CC1	10.1021/jm7014217
	24853592	155604	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	376	7	0	5	3.8	CN1CCN(c2nc3ccccc3nc2OCCCCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL406380	155604	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	376	7	0	5	3.8	CN1CCN(c2nc3ccccc3nc2OCCCCc2ccccc2)CC1	10.1021/jm7014217
	122193104	123357	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3627724	123357	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2015.08.014
	137632569	155719	0	None	4	2	Human	8.5	pKi	=	8.5	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	418	7	1	2	4.8	Ic1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4065099	155719	0	None	4	2	Human	8.5	pKi	=	8.5	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	418	7	1	2	4.8	Ic1ccc(C(OCCCc2c[nH]cn2)c2ccccc2)cc1	10.1016/j.ejmech.2016.09.074
	53233804	61182	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770966	61182	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	275	3	2	5	1.8	CN1CCN(c2cc(N)nc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2011.03.017
	54583873	61185	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770969	61185	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2011.03.017
	54584824	61193	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770977	61193	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(NC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	54585780	61214	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770998	61214	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	3	2	6	0.6	CC(C)Nc1cc(N2CCN(C)CC2)nc(N)n1	10.1016/j.bmcl.2011.03.017
	58227326	116613	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393542	116613	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	6	2	5	1.5	CCCCNc1nccc(N2CC[C@@H](NC)C2)n1	10.1016/j.bmcl.2014.12.027
	9839217	72159	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL199062	72159	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	297	1	1	3	2.6	Cc1c(Cl)sc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	54583873	61185	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL1770969	61185	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	3	5	1.3	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)c2)C1	10.1016/j.bmcl.2014.10.013
	54585781	61217	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	CHEMBL1771000	61217	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	264	4	2	6	0.9	CC(C)CNc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	71551636	89229	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	263	4	1	5	1.4	CC(C)CCc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375772	89229	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	263	4	1	5	1.4	CC(C)CCc1cc(N2CCN(C)CC2)nc(N)n1	10.1021/jm301886t
	25129527	186821	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm301886t
	CHEMBL493720	186821	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1021/jm301886t
	46881855	7649	0	None	15	4	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089040	7649	0	None	15	4	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	10967174	19227	3	None	2	2	Rat	8.5	pKi	=	8.5	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19227	3	None	2	2	Rat	8.5	pKi	=	8.5	Binding	Binding affinity of compound towards rat histamine H4 receptorBinding affinity of compound towards rat histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	46890166	7220	0	None	4786	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086105	7220	0	None	4786	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	419	6	2	4	4.5	Cc1cc(OCCCN2CCc3nc[nH]c3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	1278	2110	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	-3	8	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	71603136	89271	0	None	46	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	3	2	5	2.3	CNC1CN(c2nc(N)nc3cc(C4CCCC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376790	89271	0	None	46	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	297	3	2	5	2.3	CNC1CN(c2nc(N)nc3cc(C4CCCC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25109291	190782	9	None	-79	8	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	CHEMBL519240	190782	9	None	-79	8	Rat	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	127050309	140305	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	283	2	2	6	1.8	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1039/C6MD00108D
	CHEMBL3819470	140305	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	283	2	2	6	1.8	CN[C@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1039/C6MD00108D
	53476465	67697	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	2	2	6	2.0	NCC1CN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914547	67697	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	303	2	2	6	2.0	NCC1CN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	24737947	67770	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	283	2	2	6	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914776	67770	0	None	-	1	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	283	2	2	6	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccccc23)C1	10.1016/j.bmcl.2011.08.014
	25071004	192106	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192106	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	25071004	192106	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192106	0	None	1	2	Human	8.5	pKi	=	8.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	11957573	1971	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	1250	1971	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	3692	1971	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL19439	1971	29	None	-40	6	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm7014777
	24826358	67739	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	1	2	6	2.4	Nc1nc(N2CC3CCNC3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914745	67739	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	329	1	2	6	2.4	Nc1nc(N2CC3CCNC3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25101945	192494	0	None	3	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL522673	192494	0	None	3	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	2	2	5	1.6	CNC1CN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	58227401	175867	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4443126	175867	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596409	175867	0	None	7	2	Human	8.4	pKi	=	8.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	263	4	2	5	1.7	CN[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	25068753	187844	15	None	1	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187844	15	None	1	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1278	2110	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	-4	8	Mouse	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from mouse H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from mouse H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1267	3732	43	None	-33	28	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	3035905	3732	43	None	-33	28	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	CHEMBL260374	3732	43	None	-33	28	Human	8.4	pKi	=	8.4	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2009.08.059
	21638785	188063	4	None	4	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	CHEMBL503605	188063	4	None	4	2	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	263	1	2	2	1.9	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCNCC1	10.1021/jm800670r
	24994636	109635	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	CHEMBL3236565	109635	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	3	3	6	0.5	CN[C@H]1CN(c2cc(C3CCCC3)nc(N)n2)C[C@@H]1O	10.1021/jm401727m
	24826441	67748	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1cccc2oc3c(N4CCN(C)CC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914754	67748	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	1	7	1.7	COc1cccc2oc3c(N4CCN(C)CC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	10244243	140769	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL383384	140769	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1cccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	73353369	89269	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	6	0.4	CN1CCN(c2nc(N)nc3cccnc23)CC1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376788	89269	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	6	0.4	CN1CCN(c2nc(N)nc3cccnc23)CC1	10.1016/j.bmcl.2013.02.091
	11846636	77583	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	CHEMBL209628	77583	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(F)cccc32)CC1	10.1021/jm051008s
	155513612	175736	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4439142	175736	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4515149	175736	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	CHEMBL4595327	175736	0	None	-1	4	Mouse	7.5	pKi	=	7.5	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.0c00160
	206876	18741	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL128637	18741	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	206876	18741	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128637	18741	7	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	11249610	157412	0	None	-6309	2	Human	4.5	pKi	=	4.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	354	9	2	5	3.6	Nc1ccc(NCCc2ccc(OCCCN3CCCCC3)cc2)nc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4084820	157412	0	None	-6309	2	Human	4.5	pKi	=	4.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	354	9	2	5	3.6	Nc1ccc(NCCc2ccc(OCCCN3CCCCC3)cc2)nc1	10.1016/j.ejmech.2016.09.074
	137634777	155423	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	453	8	3	6	1.0	CCOC(=O)CN1Cc2ccccc2C[C@@H](NC(=O)C(Cc2ccccc2)C(=O)NO)C1=O	10.1016/j.ejmech.2016.09.074
	CHEMBL4061504	155423	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	453	8	3	6	1.0	CCOC(=O)CN1Cc2ccccc2C[C@@H](NC(=O)C(Cc2ccccc2)C(=O)NO)C1=O	10.1016/j.ejmech.2016.09.074
	164611474	184860	0	None	-478	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	6	4	5	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccs1	10.1016/j.ejmech.2021.113190
	CHEMBL4861704	184860	0	None	-478	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	6	4	5	2.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1cccs1	10.1016/j.ejmech.2021.113190
	164619427	185477	0	None	-7	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	5	4	4	3.1	N/C(=N\C(=O)NCCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4871221	185477	0	None	-7	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	380	5	4	4	3.1	N/C(=N\C(=O)NCCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	164627774	185973	0	None	-58	6	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	344	3	4	4	1.4	N/C(=N\C(=O)NCc1ccccc1)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	CHEMBL4878215	185973	0	None	-58	6	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	344	3	4	4	1.4	N/C(=N\C(=O)NCc1ccccc1)NC1CCc2nc(N)sc2C1	10.1016/j.ejmech.2021.113190
	24994634	2102	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2102	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2102	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2102	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2102	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2102	26	None	-60	7	Guinea pig	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from guinea pig recombinant histamine H4 receptorDisplacement of [3H]histamine from guinea pig recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	155552341	175800	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543928	175800	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595906	175800	0	None	-1	2	Human	6.5	pKi	=	6.5	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	4	3	5	1.7	CC(C)(C)CNc1nccc(NC2CCNC2)n1	10.1021/acs.jmedchem.9b01342
	44129283	199332	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3ccc(Cl)c(Cl)c3n2)CC1	10.1021/jm901379s
	CHEMBL602330	199332	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3ccc(Cl)c(Cl)c3n2)CC1	10.1021/jm901379s
	11846299	138677	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	CHEMBL378788	138677	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3cc(Cl)ccc32)CC1	10.1021/jm051008s
	10934567	19047	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	CHEMBL129026	19047	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	10934567	19047	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL129026	19047	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	25128833	187808	30	None	-4	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL501882	187808	30	None	-4	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	193	1	1	5	-0.2	CN1CCN(c2ccnc(N)n2)CC1	10.1016/j.bmcl.2011.10.104
	24826354	67783	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914790	67783	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2sc2ccc(F)cc23)C1	10.1016/j.bmcl.2011.08.014
	10934567	19047	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL129026	19047	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	271	1	1	2	2.2	Cc1cc(C)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	16006947	138515	0	None	1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL378619	138515	0	None	1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	247	6	2	2	3.1	c1nc([C@@H]2C[C@H]2CCNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	136030655	56173	0	None	-3	3	Human	7.5	pKi	=	7.5	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56173	0	None	-3	3	Human	7.5	pKi	=	7.5	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	9923162	18736	5	None	199	2	Human	7.5	pKi	=	7.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL128613	18736	5	None	199	2	Human	7.5	pKi	=	7.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	170389	205243	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634086	205243	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL91353	205243	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	1274	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	3063228	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	CHEMBL1196470	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1016/j.bmcl.2010.10.041
	170389	205243	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL4634086	205243	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL91353	205243	31	None	1	3	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.3	NCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681808	89204	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	3	8	3.1	CC(C)CNc1nccc(NCCN(C)c2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375748	89204	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	3	8	3.1	CC(C)CNc1nccc(NCCN(C)c2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	1274	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	3063228	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	CHEMBL1196470	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm201042n
	56851608	71271	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	CHEMBL1923030	71271	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	CHEMBL1963461	71271	0	None	-1	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	334	6	2	3	4.3	c1nc(CCCS/C(=N\C2CCCC2)NC2CCCCC2)c[nH]1	10.1021/jm201042n
	1274	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	3063228	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	CHEMBL1196470	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	10.1021/jm060880d
	1278	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	66572105	75270	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047263	75270	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	1	1	2	1.7	CN1CCN(C(=O)c2cc3c(F)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572106	75271	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3c(Cl)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047264	75271	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(C(=O)c2cc3c(Cl)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573463	75304	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	2	3.1	Cc1cc(Cl)cc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047454	75304	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	317	2	1	2	3.1	Cc1cc(Cl)cc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70696622	75319	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047469	75319	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10988959	117592	4	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL340740	117592	4	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cc(N)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10087533	194326	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202322	194326	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL560068	194326	0	None	-3	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	322	7	3	3	3.5	N=C(NCCc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	46929648	16565	0	None	-6	3	Human	6.5	pKi	=	6.5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	CHEMBL1243395	16565	0	None	-6	3	Human	6.5	pKi	=	6.5	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	355	5	2	3	4.3	FC(F)(F)c1ccc(SC[C@@H](c2c[nH]cn2)C2CCNCC2)cc1	10.1021/jm100643t
	137650793	156888	0	None	-1096	2	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	484	7	1	4	4.5	O=C(NCCCCN1CCN(c2cccc(Cl)c2Cl)CC1)c1cncc(Br)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4078910	156888	0	None	-1096	2	Human	5.5	pKi	=	5.5	Binding	Antagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	484	7	1	4	4.5	O=C(NCCCCN1CCN(c2cccc(Cl)c2Cl)CC1)c1cncc(Br)c1	10.1016/j.ejmech.2016.09.074
	46912442	10788	0	None	-575	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	271	4	1	3	2.9	COc1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	CHEMBL1172775	10788	0	None	-575	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO-K1 cells after 1 hr by liquid scintillation counting	ChEMBL	271	4	1	3	2.9	COc1ccc(N2CCC(Cc3c[nH]cn3)CC2)cc1	10.1016/j.bmc.2010.04.052
	57392460	69508	0	None	14	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938843	69508	0	None	14	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cccnc3[nH]2)CC1	10.1016/j.bmcl.2011.11.098
	136192886	56171	0	None	-6	4	Rat	6.5	pKi	=	6.5	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56171	0	None	-6	4	Rat	6.5	pKi	=	6.5	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1248	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	3077	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	90045	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	CHEMBL12344	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1016/j.bmcl.2010.10.041
	1239	1994	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1240	1994	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	912	1994	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL268490	1994	23	None	-154	5	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	3	2	2	0.2	CNCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	71681976	89207	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	5	4	5	2.0	Nc1nccc(NCCNC(=O)c2cc3cc(Cl)ccc3[nH]2)n1	10.1021/jm301886t
	CHEMBL2375751	89207	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	330	5	4	5	2.0	Nc1nccc(NCCNC(=O)c2cc3cc(Cl)ccc3[nH]2)n1	10.1021/jm301886t
	10023327	168439	0	None	-144	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168439	0	None	-144	3	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor N4.57Y mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11622672	74654	2	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	1	2	2.6	c1nc(CCCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203389	74654	2	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	6	1	2	2.6	c1nc(CCCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	11679919	75175	0	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204413	75175	0	None	-39	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCNCCCCCc1c[nH]cn1	10.1021/jm0504353
	11701199	75206	0	None	-3	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	1	2	2.1	CCN(CC)CCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL204615	75206	0	None	-3	2	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	1	2	2.1	CCN(CC)CCCCc1c[nH]cn1	10.1021/jm0504353
	1248	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	3077	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	90045	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	CHEMBL12344	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm060880d
	4060730	19168	51	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19168	51	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	10377814	75275	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3c(N)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047268	75275	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3c(N)cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1248	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	3077	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	90045	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	CHEMBL12344	1391	27	None	-15	7	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hrDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells after 1 hr	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1039/C2MD20212C
	4563101	66410	11	None	1	2	Human	5.5	pKi	=	5.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	265	4	2	2	2.1	CN(C)CCNC(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL185842	66410	11	None	1	2	Human	5.5	pKi	=	5.5	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	265	4	2	2	2.1	CN(C)CCNC(=O)c1cc2cc(Cl)ccc2[nH]1	10.1016/j.bmcl.2004.08.035
	71681977	89208	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	344	5	3	5	2.4	CN(CCNc1ccnc(N)n1)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1021/jm301886t
	CHEMBL2375752	89208	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	344	5	3	5	2.4	CN(CCNc1ccnc(N)n1)C(=O)c1cc2cc(Cl)ccc2[nH]1	10.1021/jm301886t
	71681479	89216	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	393	9	5	9	2.7	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375760	89216	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	393	9	5	9	2.7	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	3063453	12648	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187950	12648	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL534498	12648	1	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	189	5	5	3	-0.1	N=C(N)NCCCCSC(=N)N	10.1021/jm060880d
	112936341	136936	1	None	-12	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2nc(NCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753849	136936	1	None	-12	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2nc(NCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	164628701	185875	0	None	-186	3	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	5	4	4	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4876743	185875	0	None	-186	3	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	338	5	4	4	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC1CCCCC1	10.1016/j.ejmech.2021.113190
	57402928	69507	0	None	9	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	0	4	1.4	CN1CCN(C(=O)c2cn3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938842	69507	0	None	9	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	0	4	1.4	CN1CCN(C(=O)c2cn3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.11.098
	121238071	166676	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	271	2	1	7	0.3	CN1CCN(c2nc(N)nc(-c3ccncc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4292486	166676	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	271	2	1	7	0.3	CN1CCN(c2nc(N)nc(-c3ccncc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	11120826	18715	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128505	18715	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	11120826	18715	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128505	18715	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0341047
	49841720	69365	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3c(-c4ccccc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935557	69365	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	319	2	1	5	2.6	CN1CCN(c2nc(N)nc3c(-c4ccccc4)cccc23)CC1	10.1016/j.bmcl.2011.10.104
	44275465	98751	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL281590	98751	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	46890545	7289	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086460	7289	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	1	4	4.0	Cc1cc(OCCCN2CCN(C)CC2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	11120826	18715	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128505	18715	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(Br)cccc3[nH]2)CC1	10.1021/jm0502081
	57399829	67973	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	235	7	2	5	1.3	CN(C)CCNc1cc(NCC2CC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916505	67973	1	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	235	7	2	5	1.3	CN(C)CCNc1cc(NCC2CC2)ncn1	10.1016/j.bmcl.2011.07.114
	44126357	115402	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(N)nc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354813	115402	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	3	2	5	1.0	CN[C@@H]1CCN(c2cc(N)nc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	57393772	67820	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Br)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915041	67820	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Br)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	24854637	95859	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc3ccccc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261363	95859	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	320	3	0	5	3.2	CN1CCN(c2nc3ccccc3nc2Oc2ccccc2)CC1	10.1021/jm7014217
	11738520	138723	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	CHEMBL1794919	138723	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	CHEMBL379044	138723	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	313	0	1	4	3.4	Clc1ccc2c(c1)N=C(N1CCNCC1)c1ccccc1O2	10.1021/jm051008s
	60150162	173284	0	None	-524	5	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	479	16	2	4	4.8	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccccc1	10.1039/C8MD00527C
	CHEMBL4539444	173284	0	None	-524	5	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	479	16	2	4	4.8	N=C(N)N(CCCCN1CCN(CCCCCCCOc2ccccc2)CC1)Cc1ccccc1	10.1039/C8MD00527C
	164624107	185130	0	None	-70	6	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	9	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4865765	185130	0	None	-70	6	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	9	4	4	2.8	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCc1ccccc1	10.1016/j.ejmech.2021.113190
	164608941	183791	0	None	-75	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4845733	183791	0	None	-75	4	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	57392890	67959	0	None	-1	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	370	7	1	5	2.5	COc1cccc(C(O)(COc2ccccc2C)CN2CCN(C)CC2)c1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916492	67959	0	None	-1	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	370	7	1	5	2.5	COc1cccc(C(O)(COc2ccccc2C)CN2CCN(C)CC2)c1	10.1016/j.bmcl.2011.07.114
	21029584	188269	4	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncnc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL506969	188269	4	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncnc3ccccc23)CC1	10.1016/j.bmcl.2011.10.104
	1267	3732	43	None	-33	28	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	3035905	3732	43	None	-33	28	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	CHEMBL260374	3732	43	None	-33	28	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014777
	90655029	109633	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.4	Nc1nc(C2CCCC2)cc(N2C[C@@H]3CNC[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236563	109633	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	273	2	2	5	1.4	Nc1nc(C2CCCC2)cc(N2C[C@@H]3CNC[C@@H]3C2)n1	10.1021/jm401727m
	71518862	86057	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	342	2	2	5	2.6	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314775	86057	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	342	2	2	5	2.6	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	44547567	116604	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393533	116604	2	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	24826163	67809	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915020	67809	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	57393773	67822	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	1	7	1.8	CN1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915044	67822	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	1	7	1.8	CN1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	11572877	66730	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL187285	66730	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)c2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	44129210	197794	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	7	2	7	1.4	CNS(=O)(=O)CCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591711	197794	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	412	7	2	7	1.4	CNS(=O)(=O)CCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	156015638	177687	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4642520	177687	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4650832	177687	0	None	-58	4	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	415	10	1	2	5.5	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25179127	188481	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL509852	188481	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25179127	188481	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL509852	188481	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1ccsc1CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	71518863	86058	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	338	3	2	6	2.0	COc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314776	86058	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	338	3	2	6	2.0	COc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	8070960	155139	10	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	258	2	0	5	1.4	COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL405044	155139	10	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	258	2	0	5	1.4	COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	25130576	187033	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130576	187033	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187033	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187033	1	None	-8	4	Rat	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	86277887	123281	22	None	-	1	Human	5.5	pKi	=	5.5	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL	802	18	0	11	7.4	COc1ccc(CC[C@@H](OC(=O)[C@@H]2CCCCN2C(=O)[C@H](c2cc(OC)c(OC)c(OC)c2)C2CCCCC2)c2cccc(OCCN3CCOCC3)c2)cc1OC	10.6019/CHEMBL5058648
	CHEMBL3623630	123281	22	None	-	1	Human	5.5	pKi	=	5.5	Binding	GPCRScan assay: inhibition of H4GPCRScan assay: inhibition of H4	ChEMBL	802	18	0	11	7.4	COc1ccc(CC[C@@H](OC(=O)[C@@H]2CCCCN2C(=O)[C@H](c2cc(OC)c(OC)c(OC)c2)C2CCCCC2)c2cccc(OCCN3CCOCC3)c2)cc1OC	10.6019/CHEMBL5058648
	168294445	192398	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208113	192398	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222768	192398	0	None	-8	4	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	6	4	4	2.6	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCC2CCc3nc(N)sc3C2)cc1	10.1021/acs.jmedchem.1c00692
	25130235	186994	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	25130235	186994	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	186994	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm8005959
	CHEMBL494678	186994	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	46228076	200406	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	354	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(C(C)(C)C)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL608864	200406	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	354	4	2	6	2.7	CN1CCN(c2cc(NCc3ccc(C(C)(C)C)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228158	200463	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL609289	200463	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	4	2	7	1.3	CN1CCN(c2cc(NCc3ccc(C#N)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	57402514	67818	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Br)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915039	67818	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	375	1	2	6	2.7	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Br)cc3c2n1	10.1016/j.bmcl.2011.08.014
	164619039	185556	0	None	-17	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	4	4	4	3.0	N/C(=N\C(=O)NCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4872277	185556	0	None	-17	2	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	366	4	4	4	3.0	N/C(=N\C(=O)NCc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	57392889	67958	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	379	4	1	5	2.2	CN1CCN(c2cc(C(=O)NCc3cccc(C(F)(F)F)c3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916491	67958	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	379	4	1	5	2.2	CN1CCN(c2cc(C(=O)NCc3cccc(C(F)(F)F)c3)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	25130235	186994	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL494678	186994	1	None	1	11	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	294	2	1	6	1.3	CN1CCN(c2cc(-c3ccc(C#N)cc3)nc(N)n2)CC1	10.1021/jm800670r
	57393774	67823	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.4	Nc1nc(N2CCNCC2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915045	67823	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	1	2	7	1.4	Nc1nc(N2CCNCC2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	10083188	140390	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL382276	140390	3	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2nc3ccccc3[nH]2)CC1	10.1021/jm0502081
	1278	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	1279	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	4908365	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	CHEMBL129198	2110	79	None	-3	8	Human	7.5	pKi	=	7.5	Binding	Binding affinity to histamine 4 receptor (unknown origin)Binding affinity to histamine 4 receptor (unknown origin)	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	71603137	89272	0	None	23	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	1	2	5	1.9	CC(C)(C)c1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376791	89272	0	None	23	2	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	1	2	5	1.9	CC(C)(C)c1ccc2c(N3CCNCC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25179125	188427	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL509142	188427	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25179125	188427	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL509142	188427	0	None	-	1	Human	7.5	pKi	=	7.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3ccsc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	127051047	139768	0	None	2	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3806162	139768	0	None	2	2	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	7015108	198214	8	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	3	0	7	1.7	CN1CCN(c2ncnc3c2nnn3Cc2ccc(Cl)cc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594729	198214	8	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	3	0	7	1.7	CN1CCN(c2ncnc3c2nnn3Cc2ccc(Cl)cc2)CC1	10.1016/j.bmc.2009.08.059
	46228070	198033	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	271	4	0	5	1.1	CN1CCN(Cc2cn(Cc3ccccc3)nn2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593448	198033	0	None	-	1	Human	4.5	pKi	=	4.5	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	271	4	0	5	1.1	CN1CCN(Cc2cn(Cc3ccccc3)nn2)CC1	10.1016/j.bmc.2009.08.059
	122193100	123388	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	314	3	1	6	1.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc(F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628047	123388	0	None	-	1	Human	5.5	pKi	=	5.5	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	314	3	1	6	1.5	CN1CCN(c2nc(N)nc(/C=C/c3cccc(F)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	155513612	175736	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	1	4	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from human H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	127050018	140224	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	221	2	2	5	0.5	CN[C@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1039/C6MD00108D
	CHEMBL3818402	140224	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Inhibition of histamine H4 receptor (unknown origin)Inhibition of histamine H4 receptor (unknown origin)	ChEMBL	221	2	2	5	0.5	CN[C@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1039/C6MD00108D
	24994950	109651	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.5	CN[C@@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1021/jm401727m
	CHEMBL3236580	109651	0	None	-	1	Human	6.5	pKi	=	6.5	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.5	CN[C@@H]1CCN(c2nc(N)nc(C)c2C)C1	10.1021/jm401727m
	54583875	61211	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770995	61211	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	90655028	109627	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@H](N)C2)n1	10.1021/jm401727m
	CHEMBL3236557	109627	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	1.3	Nc1nc(C2CCCC2)cc(N2CC[C@H](N)C2)n1	10.1021/jm401727m
	59777262	109642	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236572	109642	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	283	4	2	5	1.4	CN[C@@H]1CCN(c2cc(Cc3ccccc3)nc(N)n2)C1	10.1021/jm401727m
	155512725	175657	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4437371	175657	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4594753	175657	0	None	154	2	Human	7.4	pKi	=	7.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	237	6	3	5	1.7	CC(C)(C)CNc1nccc(NCCCN)n1	10.1021/acs.jmedchem.9b01342
	46929553	16559	0	None	-5	2	Human	6.4	pKi	=	6.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2cccnc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243366	16559	0	None	-5	2	Human	6.4	pKi	=	6.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2cccnc2)c2c[nH]cn2)cc1	10.1021/jm100643t
	71519011	86023	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	376	2	2	5	3.3	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)c(Cl)c4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314496	86023	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	376	2	2	5	3.3	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Cl)c(Cl)c4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	24826598	67812	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	2	7	1.9	COc1ccc2oc3c(N4CCC[C@H](N)C4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915027	67812	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	313	2	2	7	1.9	COc1ccc2oc3c(N4CCC[C@H](N)C4)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	25178939	186257	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm800876b
	CHEMBL489464	186257	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm800876b
	24853529	95664	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	336	3	0	5	3.5	CN1CCN(c2nc3ccccc3nc2Sc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260388	95664	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	336	3	0	5	3.5	CN1CCN(c2nc3ccccc3nc2Sc2ccccc2)CC1	10.1021/jm7014217
	25178939	186257	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm901379s
	CHEMBL489464	186257	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	397	5	1	6	3.7	COc1ccc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)cc1	10.1021/jm901379s
	164611757	184310	0	None	-50	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	2.8	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4853107	184310	0	None	-50	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	2.8	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2sc(N)nc2C)cc1	10.1016/j.ejmech.2021.113190
	112936357	136821	1	None	-18	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752978	136821	1	None	-18	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)cc(-c3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	71519346	86047	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	2	6	-0.0	Cn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314764	86047	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	232	1	2	6	-0.0	Cn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	58227144	116626	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2cnnc(NC3CC4CCC3C4)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393555	116626	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2cnnc(NC3CC4CCC3C4)c2)CC1	10.1016/j.bmcl.2014.12.027
	57399018	67825	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	332	1	1	7	2.1	CN1CCCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915047	67825	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	332	1	1	7	2.1	CN1CCCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	16082831	79921	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL214311	79921	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@H]2C[C@@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	25178554	172845	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm800876b
	CHEMBL452870	172845	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm800876b
	25178554	172845	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm901379s
	CHEMBL452870	172845	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	8	2.3	Cc1noc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)n1	10.1021/jm901379s
	164610892	184605	0	None	-52	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	8	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CC1CCCCC1	10.1016/j.ejmech.2021.113190
	CHEMBL4857560	184605	0	None	-52	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	8	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CC1CCCCC1	10.1016/j.ejmech.2021.113190
	71518864	86059	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	Cc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314777	86059	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	322	2	2	5	2.3	Cc1ccc(-c2cc3c(N4CCN(C)CC4)nc(N)nc3[nH]2)cc1	10.1016/j.bmcl.2012.10.139
	122194626	123491	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629829	123491	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1ccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2015.08.014
	57399019	67829	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	2	2	7	1.4	NCC1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915051	67829	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	304	2	2	7	1.4	NCC1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	90645146	111672	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	288	2	1	6	1.0	CN1CCN(c2nc(N)nc(-c3ccc(F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290577	111672	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	288	2	1	6	1.0	CN1CCN(c2nc(N)nc(-c3ccc(F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	54584374	62779	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774590	62779	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789360	62779	0	None	-6	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	122193105	123392	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3ccc4ccccc4c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628051	123392	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	346	3	1	6	2.5	CN1CCN(c2nc(N)nc(/C=C/c3ccc4ccccc4c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	921599	19240	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0341047
	CHEMBL129660	19240	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0341047
	921599	19240	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0502081
	CHEMBL129660	19240	44	None	234	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	10.1021/jm0502081
	4060730	19168	51	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.02.097
	CHEMBL129199	19168	51	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine human recombinant histamine H4 receptorDisplacement of [3H]histamine human recombinant histamine H4 receptor	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.02.097
	25178553	172844	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm800876b
	CHEMBL452869	172844	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm800876b
	25178553	172844	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm901379s
	CHEMBL452869	172844	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	4	1	6	4.0	Cc1csc(CNc2nc(N3CCN(C)CC3)nc3ccc(Cl)cc23)c1	10.1021/jm901379s
	11846465	141388	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	CHEMBL387054	141388	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	345	0	0	4	3.9	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3cc(F)ccc32)CC1	10.1021/jm051008s
	56851606	71241	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1923029	71241	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	CHEMBL1963244	71241	0	None	-11	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	432	8	2	3	5.7	Clc1ccc(C/N=C(/NCc2ccccc2)SCCCc2c[nH]cn2)cc1Cl	10.1021/jm201042n
	51354877	58586	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCc2cn(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688988	58586	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	7	1	4	3.1	c1nc(CCCc2cn(CCC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	137658819	158751	0	None	-7585	2	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	467	9	1	5	3.9	COc1cc2c(cc1OC)C(C)=C(C(=O)NCCCCN1CCN(c3ccccc3F)CC1)C2	10.1016/j.ejmech.2016.09.074
	CHEMBL4099779	158751	0	None	-7585	2	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	467	9	1	5	3.9	COc1cc2c(cc1OC)C(C)=C(C(=O)NCCCCN1CCN(c3ccccc3F)CC1)C2	10.1016/j.ejmech.2016.09.074
	54584374	62779	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774590	62779	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789360	62779	0	None	-6	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	223	7	2	3	1.6	C=CCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	58227207	116622	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393551	116622	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	51354872	59868	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1688983	59868	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	CHEMBL1739923	59868	0	None	-323	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	291	6	0	5	2.1	c1c(CN2CCCCC2)nnn1CCCN1CCCCC1	10.1021/jm1013488
	68081433	109621	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	248	1	1	3	0.7	CN1CCN(C(=O)c2nc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	CHEMBL3236551	109621	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	248	1	1	3	0.7	CN1CCN(C(=O)c2nc3c([nH]2)CCCC3)CC1	10.1021/jm401727m
	57399747	67780	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	2	2	6	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914787	67780	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	319	2	2	6	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(F)c(F)c23)C1	10.1016/j.bmcl.2011.08.014
	25129169	186588	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186588	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	25129169	186588	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL492231	186588	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	3032915	15306	20	None	-1	7	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL12160	15306	20	None	-1	7	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	71681806	89201	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	331	8	4	9	1.0	CC(C)CNc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375667	89201	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	331	8	4	9	1.0	CC(C)CNc1cc(N(C)CCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681639	89218	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	321	6	3	7	2.3	Cc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375762	89218	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	321	6	3	7	2.3	Cc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	11492393	3985	4	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	1263	3985	4	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	CHEMBL206346	3985	4	None	-3	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	7	2	2	3.0	C(CCc1cnc[nH]1)CCNC1CCCCC1	10.1021/jm0504353
	11557610	74083	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	7	2	2	2.7	c1nc(CCCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL202744	74083	0	None	-10	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	7	2	2	2.7	c1nc(CCCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	11615406	138949	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	2	2	2.3	c1nc(CCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL379447	138949	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	2	2	2.3	c1nc(CCCCNC2CCCC2)c[nH]1	10.1021/jm0504353
	66572116	75293	1	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	2	2	1.5	Cc1cccc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047443	75293	1	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	2	2	1.5	Cc1cccc2cc(C(=O)N3CCNCC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70681961	75305	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.6	CN1CCCN(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047455	75305	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.6	CN1CCCN(C(=O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70696621	75312	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	2	2.2	O=C(c1cc2cccc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047462	75312	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	287	2	1	2	2.2	O=C(c1cc2cccc(F)c2[nH]1)N1CC(N2CCCC2)C1	10.1016/j.ejmech.2012.06.016
	70692531	75325	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.0	Cc1ccc(F)c2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047475	75325	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.0	Cc1ccc(F)c2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	24759623	95889	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm800876b
	CHEMBL261551	95889	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm800876b
	25129169	186588	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186588	0	None	-8	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	24759623	95889	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL261551	95889	16	None	18	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	318	3	0	4	3.0	CN1CCN(c2nc3ccccc3nc2Cc2ccccc2)CC1	10.1021/jm7014217
	10429016	194476	0	None	-5	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	366	7	3	3	3.6	N=C(NCCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL561250	194476	0	None	-5	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	366	7	3	3	3.6	N=C(NCCc1ccc(Br)cc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1223	938	32	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-60	11	Human	7.4	pKi	=	7.4	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	71519012	86024	0	None	218	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.8	Nc1nc(N2CCNCC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314497	86024	0	None	218	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.8	Nc1nc(N2CCNCC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	10934158	108077	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	CHEMBL320331	108077	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1016/j.bmcl.2010.10.041
	71681478	89215	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	4	8	3.2	CC(C)CCc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375759	89215	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	392	9	4	8	3.2	CC(C)CCc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)nc(N)n1	10.1021/jm301886t
	11629829	75654	0	None	-19	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	5	1	2	2.2	c1nc(CCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL205583	75654	0	None	-19	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	5	1	2	2.2	c1nc(CCCCN2CCCCC2)c[nH]1	10.1021/jm0504353
	25831	12883	26	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	CHEMBL1189742	12883	26	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	CHEMBL539343	12883	26	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	144	3	6	2	-2.0	N=C(N)NCCNC(=N)N	10.1021/jm060880d
	1278	2110	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	1279	2110	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	4908365	2110	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	CHEMBL129198	2110	79	None	-3	8	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1039/C2MD20212C
	66573456	75295	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	2	2	2	1.5	CNC1CN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047445	75295	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	2	2	2	1.5	CNC1CN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	43398194	75306	21	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	CN1CCCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047456	75306	21	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	CN1CCCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70694582	75310	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047460	75310	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	275	1	1	2	2.1	CN1CCCN(C(=O)c2cc3cccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	70686223	75315	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	2	2	2	1.4	CNC1CN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047465	75315	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	247	2	2	2	1.4	CNC1CN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	70692530	75324	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047474	75324	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	322	2	1	4	2.1	CN1CCN(C(=O)c2cc3cc(Cl)cc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1238	2043	11	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	6603865	2043	11	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	CHEMBL11919	2043	11	None	-19	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	10.1016/j.bmcl.2010.10.041
	44417445	12678	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1188154	12678	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	CHEMBL535387	12678	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	217	7	5	3	0.7	N=C(N)NCCCCCCSC(=N)N	10.1021/jm060880d
	66572102	75267	1	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ncccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047260	75267	1	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3ncccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	24854634	155603	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	400	4	0	4	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2CCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL406379	155603	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	400	4	0	4	4.5	CN1CCN(c2nc3cc(Cl)c(Cl)cc3nc2CCc2ccccc2)CC1	10.1021/jm7014217
	24853528	166518	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	329	6	0	6	1.7	CN(C)CCCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL428964	166518	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	329	6	0	6	1.7	CN(C)CCCOc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	137659490	158638	0	None	11	2	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	420	7	1	2	6.1	Brc1ccc(C(OCCCc2c[nH]cn2)c2ccc3ccccc3c2)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4098585	158638	0	None	11	2	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	420	7	1	2	6.1	Brc1ccc(C(OCCCc2c[nH]cn2)c2ccc3ccccc3c2)cc1	10.1016/j.ejmech.2016.09.074
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1007/s00044-004-0106-7
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0341047
	24803709	145385	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cc(F)cc2[nH]1	nan
	CHEMBL3917992	145385	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting methodDisplacement of [3H]-histamine from recombinant human histamine H4 receptor expressed in human SK-N-MC cells or African green monkey COS7 cell membranes after 45 mins by scintillation counting method	ChEMBL	396	6	2	5	4.3	Cc1nc(NCCCC2CCN(C)CC2)ncc1-c1nc2c(C)cc(F)cc2[nH]1	nan
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	49841846	69380	0	None	18	3	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935572	69380	0	None	18	3	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	325	2	1	6	2.7	CN1CCN(c2nc(N)nc3cc(-c4ccsc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	44129130	197865	0	None	-2	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL592379	197865	0	None	-2	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.10.104
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.03.017
	24745337	61195	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770979	61195	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	5	3	6	0.9	CN[C@@H]1CCN(c2cc(NCC(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	58227340	116603	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393532	116603	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	4	2	4	2.2	CN[C@@H]1CCN(c2ccnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm0502081
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm800876b
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	3	8	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from rat H4R expressed in HEK293T cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	44129133	197694	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	398	6	2	7	1.0	CNS(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591011	197694	0	None	-	1	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	398	6	2	7	1.0	CNS(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	1223	938	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	2790	938	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	CHEMBL14690	938	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmc.2011.03.046
	1223	938	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	2790	938	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	CHEMBL14690	938	32	None	-60	11	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1016/j.bmcl.2009.10.005
	25129169	186588	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL492231	186588	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from rat histamine H4 receptor after 1 hr by liquid scintillation counting	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	44129134	197817	0	None	22	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591969	197817	0	None	22	2	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	384	5	2	7	0.7	CN1CCN(c2nc(NCCS(N)(=O)=O)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25129169	186588	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	CHEMBL492231	186588	0	None	8	4	Rat	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	323	2	2	5	2.3	Nc1nc2c(c(N3CCNCC3)n1)CCCCC2c1ccccc1	10.1021/jm800670r
	1278	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	1279	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	4908365	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKi	=	8.4	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.5b00516
	24745506	67862	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915537	67862	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	278	5	3	6	1.3	CNC1CN(c2cc(NCCC(C)(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.07.125
	136042266	69553	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938975	69553	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	301	1	2	3	1.8	Cc1c(F)ccc2[nH]c(C(=N)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	136042268	69555	0	None	186	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938977	69555	0	None	186	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	287	1	2	3	1.5	CN1C[C@H]2CN(C(=N)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	25068753	187844	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187844	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	1278	2110	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	1279	2110	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	4908365	2110	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	CHEMBL129198	2110	79	None	3	8	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm8007618
	25068753	187844	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL502347	187844	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	2063135	8278	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1093180	8278	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1016/j.bmcl.2011.08.014
	24826437	67740	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@H]3CCCN[C@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914746	67740	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	343	1	2	6	2.8	Nc1nc(N2C[C@H]3CCCN[C@H]3C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25128823	192105	0	None	2	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	CHEMBL521995	192105	0	None	2	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	267	1	2	5	1.0	Nc1nc2c(c(N3CCNCC3)n1)Cc1ccccc1-2	10.1021/jm800670r
	1204	1893	114	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1893	114	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1893	114	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1893	114	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1893	114	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1893	114	None	-3	25	Human	8.3	pKi	=	8.3	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1278	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	1279	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	4908365	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	CHEMBL129198	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assayDisplacement of [3H]histamine from human histamine 4 receptor expressed in HEK293T cell membranes incubated for 1 hr by radioligand binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2015.12.035
	44129130	197865	0	None	-2	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592379	197865	0	None	-2	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	460	7	2	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	54581941	61213	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	288	2	2	6	0.8	Nc1nc(N2CCCC2)cc(N2CC3CCCNC3C2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770997	61213	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	288	2	2	6	0.8	Nc1nc(N2CCCC2)cc(N2CC3CCCNC3C2)n1	10.1016/j.bmcl.2011.03.017
	24826680	67771	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914777	67771	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	2	2	6	2.4	CN[C@@H]1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)C1	10.1016/j.bmcl.2011.08.014
	11601630	132283	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL370135	132283	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	283	1	1	3	2.3	CN1CCN(C(=O)c2cc3sc(Cl)cc3[nH]2)CC1	10.1021/jm0502081
	137640214	156413	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	394	6	1	2	5.5	O=C(NCCCCN1Cc2cccc3cccc(c23)C1)c1ccc2ccccc2c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4072893	156413	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	394	6	1	2	5.5	O=C(NCCCCN1Cc2cccc3cccc(c23)C1)c1ccc2ccccc2c1	10.1016/j.ejmech.2016.09.074
	71519013	86025	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	3	2	5	2.5	CCN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314498	86025	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	3	2	5	2.5	CCN1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	168284289	192294	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5194860	192294	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222123	192294	0	None	-7	4	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	428	8	4	4	3.1	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	3076002	178692	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL473099	178692	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	3076002	178692	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL473099	178692	2	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	4	0	5	3.0	CN1CCN(c2nc(OCc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	90645150	111668	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290573	111668	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	304	2	1	6	1.5	CN1CCN(c2nc(N)nc(-c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	25130576	187033	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	25130576	187033	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	CHEMBL494890	187033	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm8005959
	CHEMBL494890	187033	1	None	2	4	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	280	2	2	6	1.0	N#Cc1ccc(-c2cc(N3CCNCC3)nc(N)n2)cc1	10.1021/jm800670r
	10018335	71653	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL197477	71653	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.6	CN1CCN(C(=O)c2cc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	57393771	67817	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	311	2	2	7	1.5	COc1ccc2oc3c(N4C[C@@H]5C[C@H]4CN5)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915035	67817	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	311	2	2	7	1.5	COc1ccc2oc3c(N4C[C@@H]5C[C@H]4CN5)nc(N)nc3c2c1	10.1016/j.bmcl.2011.08.014
	10545988	62802	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774593	62802	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789558	62802	0	None	-93	2	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	46228071	198484	15	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596562	198484	15	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	283	4	1	5	1.8	CN1CCN(c2cc(NCc3ccccc3)ncn2)CC1	10.1016/j.bmc.2009.08.059
	1204	1893	114	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1893	114	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1893	114	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1893	114	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1893	114	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1893	114	None	-15	25	Mouse	7.4	pKi	=	7.4	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	46890590	6931	0	None	1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084810	6931	0	None	1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2CC3CCCNC3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57400735	67811	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	2	6	2.9	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915026	67811	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	2	6	2.9	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(C(F)(F)F)cc3c2n1	10.1016/j.bmcl.2011.08.014
	56851441	71240	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923023	71240	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963243	71240	0	None	-1	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	342	7	2	3	4.2	c1ccc(CN/C(=N/C2CCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	25147772	172827	4	None	-1	3	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL452847	172827	4	None	-1	3	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2015.12.035
	16061961	197987	0	None	7	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197987	0	None	7	2	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	10381810	203453	0	None	-3	6	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL7828	203453	0	None	-3	6	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3cc(Cl)ccc3Oc3ccccc32)CC1	10.1021/jm051008s
	137647794	157426	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	464	8	1	6	4.2	COc1ccc2c(c1)c(CC(=O)NCCCn1ccnc1)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4085023	157426	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Antagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at human H4 receptor expressed in CHO cells co-expressing Galphai2 assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	464	8	1	6	4.2	COc1ccc2c(c1)c(CC(=O)NCCCn1ccnc1)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	10545988	62802	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774593	62802	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789558	62802	0	None	-93	2	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	237	8	2	3	2.0	C=CCCCNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	122193096	123384	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628043	123384	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645154	111676	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	1.9	CN1CCN(c2nc(N)nc(-c3ccc(C(F)(F)F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290581	111676	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	1.9	CN1CCN(c2nc(N)nc(-c3ccc(C(F)(F)F)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10777275	5803	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079553	5803	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL156005	5803	1	None	-22	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	1267	3732	43	None	-33	28	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	3035905	3732	43	None	-33	28	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	CHEMBL260374	3732	43	None	-33	28	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2013.06.075
	127036978	136744	0	None	1	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752225	136744	0	None	1	2	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	415	5	1	6	3.6	COc1ccc(CNc2nc3c(c(N4CCN(C)CC4)n2)CCc2ccccc2-3)cc1	10.1016/j.bmcl.2015.12.035
	164612037	184785	0	None	-81283	20	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	326	8	4	4	1.8	CCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4860528	184785	0	None	-81283	20	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	326	8	4	4	1.8	CCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	1224	1399	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	3100	1399	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	8980	1399	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	916	1399	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	CHEMBL657	1399	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	DB01075	1399	79	None	-194	13	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	10.1016/j.bmcl.2011.09.001
	9883231	112874	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.0	CN1CCN(C(=O)c2nc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL331773	112874	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	312	1	1	3	2.0	CN1CCN(C(=O)c2nc3cc(C(F)(F)F)ccc3[nH]2)CC1	10.1021/jm0502081
	1267	3732	43	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	3035905	3732	43	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	CHEMBL260374	3732	43	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmc.2011.03.046
	1267	3732	43	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	3035905	3732	43	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	CHEMBL260374	3732	43	None	-33	28	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2009.10.005
	24992735	109653	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	CHEMBL3236582	109653	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	247	2	2	5	0.7	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC3)C1	10.1021/jm401727m
	24826081	67756	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914762	67756	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	1	1	6	2.9	CN1CCN(c2nc(N)nc3c2sc2cc(Br)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	90645153	111675	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290580	111675	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	284	2	1	6	1.2	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.ejmech.2014.06.032
	25178344	186293	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489664	186293	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10934158	108077	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	CHEMBL320331	108077	1	None	-1	2	Human	6.4	pKi	=	6.4	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	258	5	1	3	2.8	c1ccc(COC[C@@H]2CC[C@H](c3c[nH]cn3)O2)cc1	10.1021/jm0300025
	24853593	95599	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccncc2)CC1	10.1021/jm7014217
	CHEMBL259956	95599	0	None	-	1	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccncc2)CC1	10.1021/jm7014217
	54586765	61198	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(NCCO)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770982	61198	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	252	5	4	7	-0.7	CN[C@@H]1CCN(c2cc(NCCO)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	44275465	98751	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2013.02.091
	CHEMBL281590	98751	2	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	243	1	1	5	1.0	CN1CCN(c2nc(N)nc3ccccc23)CC1	10.1016/j.bmcl.2013.02.091
	24826080	67758	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	1	1	6	3.2	CN1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914764	67758	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	367	1	1	6	3.2	CN1CCN(c2nc(N)nc3c2sc2cc(C(F)(F)F)ccc23)CC1	10.1016/j.bmcl.2011.08.014
	71519349	86050	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	6	1.8	Cn1c(-c2ccc(F)cc2)cc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314767	86050	0	None	-	1	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	326	2	2	6	1.8	Cn1c(-c2ccc(F)cc2)cc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	57393770	67814	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915031	67814	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	315	1	2	6	2.2	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	155534519	175899	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4470527	175899	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596640	175899	0	None	-47	2	Human	6.4	pKi	=	6.4	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	288	7	3	5	2.7	CC(C)(C)CNc1nccc(NCCCc2c[nH]cn2)n1	10.1021/acs.jmedchem.9b01342
	51354873	58583	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688984	58583	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	267	6	1	4	2.2	c1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	51354874	58584	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	335	6	1	4	3.5	Clc1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1Cl	10.1021/jm1013488
	CHEMBL1688985	58584	0	None	-	1	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	335	6	1	4	3.5	Clc1ccc(Cn2cc(CCCc3c[nH]cn3)nn2)cc1Cl	10.1021/jm1013488
	168292954	192385	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5202592	192385	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222660	192385	0	None	-112	6	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	388	8	4	4	2.5	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2cnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	11779175	62821	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774600	62821	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789801	62821	0	None	-5	3	Human	5.4	pKi	=	5.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164612760	184125	0	None	-38	4	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4850490	184125	0	None	-38	4	Human	4.4	pKi	=	4.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	390	7	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)NC[C@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	11779175	62821	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774600	62821	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789801	62821	0	None	-5	3	Human	6.4	pKi	=	6.4	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	293	8	2	3	3.4	O=C(NCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	58227085	116616	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393545	116616	0	None	-	1	Human	7.4	pKi	=	7.4	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@H]1CCN(c2ccnc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2014.12.027
	1267	3732	43	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	3035905	3732	43	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	CHEMBL260374	3732	43	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysisDisplacement of [3H]-Nalpha-Methylhistamine from human recombinant histamine H4 receptor expressed in CHO cell membranes after 1 hrs by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1039/C8MD00527C
	11846464	78036	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	CHEMBL211170	78036	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	361	0	0	4	4.4	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3c(Cl)cccc32)CC1	10.1021/jm051008s
	118720433	115397	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	206	3	1	4	0.8	CCc1cc(N2CC[C@@H](NC)C2)ncn1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354807	115397	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	206	3	1	4	0.8	CCc1cc(N2CC[C@@H](NC)C2)ncn1	10.1016/j.bmcl.2014.10.013
	51354581	58574	0	None	-4	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	275	10	1	4	3.1	CCCCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688973	58574	0	None	-4	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	275	10	1	4	3.1	CCCCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	9877454	203891	1	None	-4	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	CHEMBL81644	203891	1	None	-4	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1ccc(Cc2c[nH]cn2)nc1	10.1021/jm049932u
	1613	2306	44	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	205	2306	44	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	3964	2306	44	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	CHEMBL831	2306	44	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	DB00408	2306	44	None	-134	45	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	10.1021/jm051008s
	164609493	183844	0	None	-2630	6	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	350	6	4	5	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccco1	10.1016/j.ejmech.2021.113190
	CHEMBL4846380	183844	0	None	-2630	6	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	350	6	4	5	1.9	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccco1	10.1016/j.ejmech.2021.113190
	71518698	86052	2	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	218	1	3	5	-0.1	Nc1nc(N2CCNCC2)c2cc[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314770	86052	2	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	218	1	3	5	-0.1	Nc1nc(N2CCNCC2)c2cc[nH]c2n1	10.1016/j.bmcl.2012.10.139
	4242975	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	CHEMBL128088	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1007/s00044-004-0106-7
	4242975	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	CHEMBL128088	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0341047
	16061961	197987	0	None	7	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593103	197987	0	None	7	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	316	4	2	6	1.6	CN1CCN(c2cc(NCc3ccc(F)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	11258458	83026	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	CHEMBL219930	83026	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	412	6	1	4	4.5	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	57398183	67695	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	347	4	3	7	1.8	Nc1nc(N2CC[C@@H](NCCO)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914545	67695	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	347	4	3	7	1.8	Nc1nc(N2CC[C@@H](NCCO)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	4242975	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	CHEMBL128088	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1021/jm0502081
	118720438	115403	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	220	3	1	4	1.4	CN[C@@H]1CCN(c2ccnc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354814	115403	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	220	3	1	4	1.4	CN[C@@H]1CCN(c2ccnc(C(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	24854640	95600	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccccn2)CC1	10.1021/jm7014217
	CHEMBL259957	95600	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	335	4	0	6	2.4	CN1CCN(c2nc3ccccc3nc2OCc2ccccn2)CC1	10.1021/jm7014217
	135589846	141276	3	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	356	2	2	5	3.1	OCCN1CCN(C2=Nc3cc(Cl)ccc3Nc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL386333	141276	3	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	356	2	2	5	3.1	OCCN1CCN(C2=Nc3cc(Cl)ccc3Nc3ccccc32)CC1	10.1021/jm051008s
	24826439	67765	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	2	2	7	1.4	COc1cccc2oc3c(N4CCNCC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	CHEMBL1914771	67765	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	299	2	2	7	1.4	COc1cccc2oc3c(N4CCNCC4)nc(N)nc3c12	10.1016/j.bmcl.2011.08.014
	10446295	2092	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	1277	2092	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL185951	2092	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.07.114
	10446295	2092	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	1277	2092	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL185951	2092	29	None	269	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2011.11.098
	10023327	168439	0	None	-144	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168439	0	None	-144	3	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	1267	3732	43	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	3035905	3732	43	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	CHEMBL260374	3732	43	None	-33	28	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm8003625
	155556721	173972	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	248	2	1	6	0.6	CC(C)=Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4556243	173972	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	248	2	1	6	0.6	CC(C)=Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	24854639	95676	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	5	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260458	95676	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	348	5	0	5	3.0	CN1CCN(c2nc3ccccc3nc2OCCc2ccccc2)CC1	10.1021/jm7014217
	11344458	203477	2	None	-14	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78497	203477	2	None	-14	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	187	4	1	2	2.0	c1cncc(CCCc2c[nH]cn2)c1	10.1021/jm049932u
	51354588	59840	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	CHEMBL1688980	59840	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	CHEMBL1739759	59840	0	None	3	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	6	0	5	2.1	c1c(CC2CCCCC2)nnn1CCCN1CCOCC1	10.1021/jm1013488
	16106	117630	28	None	-20	12	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	10.1021/jm051008s
	CHEMBL340801	117630	28	None	-20	12	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	10.1021/jm051008s
	46881855	7649	0	None	-15	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	CHEMBL1089040	7649	0	None	-15	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	337	3	2	5	2.5	CN[C@@H]1CCN(c2nc(N)nc3c2CCCC(c2ccccc2)C3)C1	10.1016/j.bmcl.2010.01.131
	71519014	86026	0	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	2	5	2.9	CC(C)N1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314499	86026	0	None	-	1	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	354	3	2	5	2.9	CC(C)N1CCN(c2nc(N)nc3[nH]c(-c4ccc(F)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	122193094	123382	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628041	123382	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	155513612	175736	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4439142	175736	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4515149	175736	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	CHEMBL4595327	175736	0	None	-1	4	Mouse	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of [3H]-UR-DEBa176 from mouse H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2ccnc(NCC(C)(C)C)n2)C1	10.1021/acs.jmedchem.9b01342
	45379808	199524	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	362	5	2	6	1.6	CC(=O)NCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL603621	199524	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	362	5	2	6	1.6	CC(=O)NCCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	168286426	192314	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5198795	192314	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222241	192314	0	None	-109	6	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	403	10	4	5	2.3	CC(CCCc1ccccc1)CNC(=O)/N=C(\N)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	164620139	185196	0	None	-56	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	436	8	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4866734	185196	0	None	-56	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	436	8	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	136042267	69554	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938976	69554	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	11623296	116509	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL338972	116509	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	280	1	1	3	1.2	CN1CCN(C(=O)c2nc3c(F)c(F)ccc3[nH]2)CC1	10.1021/jm0502081
	11066922	19210	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129436	19210	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	11066922	19210	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129436	19210	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0341047
	49841845	69381	0	None	19	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935573	69381	0	None	19	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccco4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	25068753	187844	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.10.104
	CHEMBL502347	187844	15	None	-1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	295	1	2	5	1.8	Nc1nc2c(c(N3CC[C@@H](N)C3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.10.104
	1223	938	32	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	2790	938	32	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	CHEMBL14690	938	32	None	-60	11	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm7014777
	1278	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	1278	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	1279	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	1279	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	4908365	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	4908365	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	CHEMBL129198	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.10.013
	CHEMBL129198	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2014.12.027
	58227086	116628	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393557	116628	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	1279	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	4908365	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	CHEMBL129198	2110	79	None	-3	8	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm401727m
	2063135	8278	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1021/jm401727m
	CHEMBL1093180	8278	9	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	302	1	0	5	2.8	CN1CCN(c2ncnc3c2oc2ccc(Cl)cc23)CC1	10.1021/jm401727m
	23650959	109619	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	410	7	1	4	5.3	Cc1cnc(OCCCCC2CCN(C)CC2)cc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/jm401727m
	CHEMBL3236548	109619	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	410	7	1	4	5.3	Cc1cnc(OCCCCC2CCN(C)CC2)cc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/jm401727m
	11066922	19210	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129436	19210	1	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1021/jm0502081
	155524115	175963	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4454158	175963	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	CHEMBL4597218	175963	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.0c00160
	70696620	75300	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	1	2	3	1.8	CN1CCN(C(=O)c2cc3cc(Cl)cc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047450	75300	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	292	1	2	3	1.8	CN1CCN(C(=O)c2cc3cc(Cl)cc(N)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	25129523	186694	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	CHEMBL492884	186694	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm800670r
	76311195	105558	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	284	2	2	6	1.1	CN1CCN(c2cc(-c3ccc(N)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL3133130	105558	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	284	2	2	6	1.1	CN1CCN(c2cc(-c3ccc(N)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25129523	186694	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	186694	40	None	1	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25128824	192565	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192565	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	155524115	175963	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4454158	175963	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597218	175963	0	None	-2	3	Human	8.3	pKi	=	8.3	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	286	3	2	5	2.2	CC(C)(C)CNc1nccc(N2CCc3nc[nH]c3C2)n1	10.1021/acs.jmedchem.9b01342
	25128824	192565	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	CHEMBL523225	192565	0	None	-3	2	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	313	1	2	5	1.8	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccc(F)cc1-2	10.1021/jm800670r
	25071004	192106	0	None	-1	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	CHEMBL521996	192106	0	None	-1	2	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	309	2	2	5	2.0	CN[C@@H]1CCN(c2nc(N)nc3c2CCCc2ccccc2-3)C1	10.1021/jm800670r
	11705395	15861	0	None	-1	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	CHEMBL1223749	15861	0	None	-1	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.6	Clc1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1Cl	10.1016/j.ejmech.2016.09.074
	24826162	67785	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914792	67785	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@@H]1CCN(c2nc(N)nc3c2sc2cc(Cl)ccc23)C1	10.1016/j.bmcl.2011.08.014
	71519674	89285	0	None	1	5	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376804	89285	0	None	1	5	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	270	3	2	6	0.9	CNC1CN(c2nc(N)nc3cc(C4CC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	46890072	6817	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084355	6817	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	404	8	2	4	4.9	Cc1cc(OCCCNCc2cccnc2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	24994634	2102	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	8984	2102	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	CHEMBL3236549	2102	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/jm401727m
	24994634	2102	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	8984	2102	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	CHEMBL3236549	2102	26	None	2	7	Mouse	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from mouse recombinant histamine H4 receptorDisplacement of [3H]histamine from mouse recombinant histamine H4 receptor	ChEMBL	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	10.1021/acs.jmedchem.5b00516
	44129056	197909	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	474	7	1	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592663	197909	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	474	7	1	7	2.9	CN1CCN(c2nc(NCCS(=O)(=O)N(C)c3ccccc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25128822	186888	0	None	10	5	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	CHEMBL494093	186888	0	None	10	5	Rat	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	1	5	1.6	CN1CCN(c2nc(N)nc3c2CCc2ccccc2-3)CC1	10.1021/jm800670r
	11554490	15862	0	None	-5	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.3	FC(F)(F)c1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL1223750	15862	0	None	-5	4	Human	8.3	pKi	=	8.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	428	7	1	4	5.3	FC(F)(F)c1cccc(N2CCN(CCCCNc3nccc4ccccc34)CC2)c1	10.1016/j.ejmech.2016.09.074
	24797956	109628	3	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	CHEMBL3236558	109628	3	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	233	2	2	5	0.9	Nc1nc(C2CCCC2)cc(N2CC(N)C2)n1	10.1021/jm401727m
	24825514	186262	0	None	5	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	256	4	1	2	3.6	CC(C)(C)CC(=O)c1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	CHEMBL489493	186262	0	None	5	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	256	4	1	2	3.6	CC(C)(C)CC(=O)c1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	24825515	186724	0	None	-1	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	238	2	1	1	3.4	CC(C)(C)C#Cc1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	CHEMBL493014	186724	0	None	-1	3	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	238	2	1	1	3.4	CC(C)(C)C#Cc1cccc(Cc2c[nH]cn2)c1	10.1021/jm7014149
	11301441	95694	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm800876b
	CHEMBL260549	95694	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm800876b
	11301441	95694	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL260549	95694	6	None	-	1	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1021/jm7014217
	24825791	67698	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914548	67698	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	25109291	190782	9	None	-501	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	CHEMBL519240	190782	9	None	-501	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells by liquid scintillation counting	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1021/jm8007618
	45379922	197872	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	552	7	2	7	2.8	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(I)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL592434	197872	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	552	7	2	7	2.8	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3cc(I)ccc3n2)CC1	10.1021/jm901379s
	54585777	61190	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	5	3	5	2.1	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770974	61190	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	297	5	3	5	2.1	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)c2)C1	10.1016/j.bmcl.2011.03.017
	118720441	115407	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	3	4	2.5	Nc1cc(C2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354819	115407	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	3	4	2.5	Nc1cc(C2CCNCC2)cc(NC2CCCC2)n1	10.1016/j.bmcl.2014.10.013
	24826518	67747	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2cccc(C(F)(F)F)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914753	67747	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	2.7	CN1CCN(c2nc(N)nc3c2oc2cccc(C(F)(F)F)c23)CC1	10.1016/j.bmcl.2011.08.014
	1278	2110	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	1279	2110	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	4908365	2110	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	CHEMBL129198	2110	79	None	-3	8	Human	7.3	pKi	=	7.3	Binding	Binding affinity to histamine H4 receptorBinding affinity to histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmc.2009.08.059
	136192886	56171	0	None	1	4	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632411	56171	0	None	1	4	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	257	2	2	3	2.7	CN1CCC(/C(=N/O)c2cc3ccccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136141537	56172	1	None	-2	3	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632412	56172	1	None	-2	3	Human	7.3	pKi	=	7.3	Binding	Binding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to human recombinant histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	271	2	2	3	3.0	Cc1ccc2[nH]c(/C(=N\O)C3CCN(C)CC3)cc2c1	10.1016/j.bmcl.2010.10.041
	71525678	89281	0	None	-5	6	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89281	0	None	-5	6	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	1267	3732	43	None	-14	28	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	3035905	3732	43	None	-14	28	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	CHEMBL260374	3732	43	None	-14	28	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm100064d
	1223	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1269	121	33	None	213	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	37463	121	33	None	213	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL275443	121	33	None	213	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	10.1016/j.bmcl.2010.10.041
	1255	3134	11	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	6421522	3134	11	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL19173	3134	11	None	-12	4	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	216	6	1	2	2.6	C(CCc1cnc[nH]1)OCc1ccccc1	10.1016/j.bmcl.2010.10.041
	10017000	202770	0	None	1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL72431	202770	0	None	1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	288	7	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1223	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	1223	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	2790	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	CHEMBL14690	938	32	None	-60	11	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]JNJ7777120 from human H4 receptor E5.46Q mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/jm201042n
	71681644	89223	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	412	9	4	8	3.8	CC(C)CNc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375767	89223	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	412	9	4	8	3.8	CC(C)CNc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	71681805	89228	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	426	9	3	8	3.8	CC(C)CNc1nccc(NCCN(C)c2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375771	89228	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	426	9	3	8	3.8	CC(C)CNc1nccc(NCCN(C)c2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	11506928	74078	0	None	-1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	2	2	3.0	c1nc(CCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL202728	74078	0	None	-1	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	235	6	2	2	3.0	c1nc(CCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	11600779	75680	0	None	-6	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205731	75680	0	None	-6	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	7	2	2	1.7	CCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	11536119	138165	0	None	-15	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	249	7	2	2	3.4	c1nc(CCCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL377954	138165	0	None	-15	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	249	7	2	2	3.4	c1nc(CCCCCNC2CCCCCC2)c[nH]1	10.1021/jm0504353
	4242975	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	CHEMBL128088	18639	9	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	1	1	2	1.9	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1016/j.ejmech.2012.06.016
	45379921	197514	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	426	6	1	7	3.6	CN1CCN(c2nc(NCCc3ccc([N+](=O)[O-])cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL589800	197514	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	426	6	1	7	3.6	CN1CCN(c2nc(NCCc3ccc([N+](=O)[O-])cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	9972181	193537	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202331	193537	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL550757	193537	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	302	8	3	3	3.2	N=C(NCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10063835	62778	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774601	62778	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789356	62778	0	None	-2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	46890200	7066	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	442	9	1	4	5.4	CN(C)CCN(C)CCCOc1ccc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)cc1Cl	10.1016/j.bmcl.2010.04.017
	CHEMBL1085403	7066	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	442	9	1	4	5.4	CN(C)CCN(C)CCCOc1ccc(-c2nc3cc(C(C)(C)C)ccc3[nH]2)cc1Cl	10.1016/j.bmcl.2010.04.017
	1267	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-15	25	Mouse	6.3	pKi	=	6.3	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1267	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1267	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	71681803	89172	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)ncn1	10.1021/jm301886t
	CHEMBL2375363	89172	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1cc(NCCNc2cc(-c3ccccc3)nc(N)n2)ncn1	10.1021/jm301886t
	11672774	3986	6	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	1245	3986	6	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	CHEMBL206548	3986	6	None	-31	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	181	6	1	2	1.7	CN(CCCCCc1[nH]cnc1)C	10.1021/jm0504353
	11564628	75165	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	193	5	1	2	1.8	c1nc(CCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL204360	75165	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	193	5	1	2	1.8	c1nc(CCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	11629931	75943	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	5	1	2	2.6	c1nc(CCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL205868	75943	0	None	-12	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	221	5	1	2	2.6	c1nc(CCCCN2CCCCCC2)c[nH]1	10.1021/jm0504353
	70686222	75314	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	2	2	2	1.7	CN[C@@H]1CCN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047464	75314	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	261	2	2	2	1.7	CN[C@@H]1CCN(C(=O)c2cc3cccc(F)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	135398737	942	89	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	38	942	89	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	722	942	89	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	CHEMBL42	942	89	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	DB00363	942	89	None	-57	92	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1 hr by liquid scintillation counting analysis	ChEMBL	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	10.1039/C2MD20212C
	44395314	123156	0	None	-3	2	Human	5.3	pKi	=	5.3	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	2	2	2	3.2	CN1CCC(C(O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL362163	123156	0	None	-3	2	Human	5.3	pKi	=	5.3	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	2	2	2	3.2	CN1CCC(C(O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	126688	400	28	None	1	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	4025	400	28	None	1	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	CHEMBL293762	400	28	None	1	4	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	157	2	2	4	0.5	NCCc1sc(nc1C)N	10.1016/j.bmcl.2010.10.041
	66572110	75276	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047269	75276	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	285	2	1	3	1.8	CC(=O)c1cccc2[nH]c(C(=O)N3CCN(C)CC3)cc12	10.1016/j.ejmech.2012.06.016
	70684085	75307	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	325	1	1	2	3.0	CN1CCCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047457	75307	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	325	1	1	2	3.0	CN1CCCN(C(=O)c2cc3cccc(C(F)(F)F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10063835	62778	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774601	62778	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789356	62778	0	None	-2	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	9	2	3	3.8	O=C(NCCCCC1CCCCC1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	206876	18741	7	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1039/C2MD20212C
	CHEMBL128637	18741	7	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	273	2	1	3	1.6	COc1ccc2[nH]c(C(=O)N3CCN(C)CC3)cc2c1	10.1039/C2MD20212C
	57395620	67859	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915534	67859	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.125
	57395620	67859	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915534	67859	0	None	15	3	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	90645160	111682	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3c(F)cccc3F)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290587	111682	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	306	2	1	6	1.2	CN1CCN(c2nc(N)nc(-c3c(F)cccc3F)n2)CC1	10.1016/j.ejmech.2014.06.032
	57390145	69548	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938970	69548	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	290	1	2	3	1.5	O=C(c1nc2cc(Cl)ccc2[nH]1)N1C[C@@H]2CNC[C@@H]2C1	10.1016/j.bmcl.2011.11.098
	49865668	15860	0	None	-51	4	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	390	8	1	5	4.3	COc1ccccc1N1CCN(CCCCNc2nccc3ccccc23)CC1	10.1016/j.ejmech.2016.09.074
	CHEMBL1223748	15860	0	None	-51	4	Human	7.3	pKi	=	7.3	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	390	8	1	5	4.3	COc1ccccc1N1CCN(CCCCNc2nccc3ccccc23)CC1	10.1016/j.ejmech.2016.09.074
	54586764	61194	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N(C)C(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770978	61194	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	4	2	6	0.7	CN[C@@H]1CCN(c2cc(N(C)C(C)C)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	44546966	116596	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	4	1	4	1.9	CC(C)CNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393525	116596	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	4	1	4	1.9	CC(C)CNc1cc(N2CCN(C)CC2)ccn1	10.1016/j.bmcl.2014.12.027
	9964223	203964	11	None	-36	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL82244	203964	11	None	-36	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	173	3	1	2	1.6	c1cncc(CCc2c[nH]cn2)c1	10.1021/jm049932u
	90645148	111670	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290575	111670	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	295	2	1	7	0.7	CN1CCN(c2nc(N)nc(-c3cccc(C#N)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	56950378	69510	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	CHEMBL1938845	69510	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	302	1	1	3	1.6	Cc1c(F)ccc2[nH]c(C(=O)N3C[C@@H]4CN(C)C[C@@H]4C3)nc12	10.1016/j.bmcl.2011.11.098
	25071940	186761	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	186761	0	None	-5	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	90645145	111673	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL3290578	111673	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	66741667	109636	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3236566	109636	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	122194623	123488	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	296	3	1	6	1.4	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629826	123488	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	296	3	1	6	1.4	CN1CCN(c2nc(N)nc(/C=C/c3ccccc3)n2)CC1	10.1016/j.ejmech.2015.08.014
	90645145	111673	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290578	111673	0	None	28	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	348	2	1	6	1.6	CN1CCN(c2nc(N)nc(-c3ccc(Br)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	10083861	15317	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)nc2c1	10.1021/jm0502081
	CHEMBL121679	15317	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	1	3	1.3	Cc1ccc2[nH]c(C(=O)N3CCN(C)CC3)nc2c1	10.1021/jm0502081
	121238067	166547	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4290148	166547	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	57394636	67689	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	284	1	0	5	2.6	CN1CCN(c2ncnc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914534	67689	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	284	1	0	5	2.6	CN1CCN(c2ncnc3c2sc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	71525587	89283	0	None	177	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	255	2	2	7	-0.1	CNC1CN(c2nc(N)nc3cc(C#N)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376802	89283	0	None	177	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	255	2	2	7	-0.1	CNC1CN(c2nc(N)nc3cc(C#N)cnc23)C1	10.1016/j.bmcl.2013.02.091
	753256	178760	10	None	2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL473662	178760	10	None	2	2	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	208	1	2	6	-0.6	CN1CCN(c2cc(N)nc(N)n2)CC1	10.1016/j.bmcl.2012.10.139
	46228143	198390	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL595880	198390	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	343	5	2	8	1.3	CN1CCN(c2cc(NCc3cccc([N+](=O)[O-])c3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	24745335	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	-6	5	Mouse	7.3	pKi	=	7.3	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	24745335	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	8985	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	CHEMBL1915540	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	DB15027	292	7	None	-4	5	Rat	7.3	pKi	=	7.3	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2013.02.091
	51354583	58576	0	None	2	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.1	c1nc(CCCn2cc(CC3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688975	58576	0	None	2	2	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	287	6	1	4	3.1	c1nc(CCCn2cc(CC3CCCCCC3)nn2)c[nH]1	10.1021/jm1013488
	71518860	86055	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	2	2	5	2.0	CN1CCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314773	86055	0	None	-	1	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	308	2	2	5	2.0	CN1CCN(c2nc(N)nc3[nH]c(-c4ccccc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	46228113	200379	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	328	5	2	7	1.4	COc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	CHEMBL608717	200379	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	328	5	2	7	1.4	COc1ccc(CNc2cc(N3CCN(C)CC3)nc(N)n2)cc1	10.1016/j.bmc.2009.08.059
	164611807	184391	0	None	-162	4	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4854278	184391	0	None	-162	4	Human	4.3	pKi	=	4.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	376	6	4	4	2.5	Cc1nc(N)sc1CCCN/C(N)=N/C(=S)N[C@@H](C)c1ccccc1	10.1016/j.ejmech.2021.113190
	49841718	69377	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)cc1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935569	69377	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2ccc3c(N4CCN(C)CC4)nc(N)nc3c2)cc1	10.1016/j.bmcl.2011.10.104
	58227143	116612	1	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393541	116612	1	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	1	5	1.4	CCCCNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	67087450	116621	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393550	116621	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	5	2	5	1.3	CN[C@@H]1CCN(c2cnnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	46890071	6816	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.7	Cc1cc(OCCCNCC2CCCN(C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1084354	6816	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	424	8	2	4	4.7	Cc1cc(OCCCNCC2CCCN(C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	66740470	109297	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	CHEMBL3233048	109297	0	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	289	2	2	6	0.9	Nc1nc(C2CCCC2)cc(N2C[C@H]3NCCO[C@@H]3C2)n1	10.1021/jm401727m
	2306318	86566	12	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL232421	86566	12	None	-	1	Human	7.3	pKi	=	7.3	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	268	1	0	5	2.1	CN1CCN(c2ncnc3c2oc2ccccc23)CC1	10.1016/j.bmcl.2011.08.014
	121238068	156656	0	None	-281	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	1	6	2.0	CN1CCN(c2nc(N)nc(Cc3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4076046	156656	0	None	-281	2	Human	5.3	pKi	=	5.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	334	3	1	6	2.0	CN1CCN(c2nc(N)nc(Cc3cccc4ccccc34)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178341	186217	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489271	186217	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178341	186217	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489271	186217	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	382	4	2	6	3.2	CN1CCN(c2nc(NCc3ccc(N)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	25178556	188486	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL509961	188486	0	None	-	1	Human	6.3	pKi	=	6.3	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	423	4	1	6	4.9	CN1CCN(c2nc(NCc3cc4ccccc4s3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	23649429	95837	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	2	0	5	2.7	COc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL261224	95837	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	326	2	0	5	2.7	COc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	44478385	5684	2	None	9	4	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	CHEMBL1078642	5684	2	None	9	4	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	342	9	3	4	2.5	N#C/N=C(/NCCCCc1c[nH]cn1)NCCSc1ccccc1	10.1021/jm900526h
	25178344	186293	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489664	186293	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	321	4	2	6	1.4	CN1CCN(c2nc(NCCO)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	24853597	95836	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1cccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)c1	10.1021/jm7014217
	CHEMBL261223	95836	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	334	3	0	5	3.5	Cc1cccc(Oc2nc3ccccc3nc2N2CCN(C)CC2)c1	10.1021/jm7014217
	8070044	155399	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	300	4	0	5	2.4	CC(C)COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL406123	155399	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	300	4	0	5	2.4	CC(C)COc1nc2ccccc2nc1N1CCN(C)CC1	10.1021/jm7014217
	16061844	61197	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(NCc3ccccc3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770981	61197	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(NCc3ccccc3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	57403351	67966	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2cc(NCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916499	67966	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN(C)[C@@H]1CCN(c2cc(NCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	156011443	177756	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4636487	177756	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651225	177756	0	None	-50	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	387	8	1	2	4.7	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	25178339	186191	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489061	186191	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354585	58578	0	None	-5	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	245	5	1	4	2.0	c1nc(CCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688977	58578	0	None	-5	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	245	5	1	4	2.0	c1nc(CCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	51354876	59869	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1739924	59869	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL3216267	59869	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	12741697	95700	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL260565	95700	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	25178339	186191	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489061	186191	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	5	4.0	CN1CCN(c2nc(NCC3CCCCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	919019	136196	7	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2[nH]c3c(N4CCN(C)CC4)ncnc3c2c1	10.1039/C5MD00022J
	CHEMBL3740837	136196	7	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2[nH]c3c(N4CCN(C)CC4)ncnc3c2c1	10.1039/C5MD00022J
	929854	197397	8	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	289	4	2	4	2.7	CN(C)CCNc1ncnc2c1[nH]c1ccc(Cl)cc12	10.1039/C5MD00022J
	CHEMBL588176	197397	8	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	289	4	2	4	2.7	CN(C)CCNc1ncnc2c1[nH]c1ccc(Cl)cc12	10.1039/C5MD00022J
	168278543	192239	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5184846	192239	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221764	192239	0	None	-4	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	358	4	4	4	1.6	N/C(=N\C(=O)NCc1ccccc1)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	168295803	192433	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5207206	192433	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222937	192433	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	338	6	4	4	1.6	CCCCCNC(=O)/N=C(\N)NCC1CCc2nc(N)sc2C1	10.1021/acs.jmedchem.1c00692
	57402471	67519	0	None	-549	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	8	1	5	4.0	COc1ccc(CN(CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910378	67519	0	None	-549	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	469	8	1	5	4.0	COc1ccc(CN(CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	164614645	184539	0	None	-57	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	CHEMBL4856572	184539	0	None	-57	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	402	8	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	57394627	67964	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	5	2	5	2.4	CN1CC[C@H](Nc2cc(NCCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916497	67964	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	5	2	5	2.4	CN1CC[C@H](Nc2cc(NCCC(C)(C)C)ncn2)C1	10.1016/j.bmcl.2011.07.114
	71519179	86045	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	3	4	5	2.1	Nc1nc(N[C@H]2CCNC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314762	86045	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	3	4	5	2.1	Nc1nc(N[C@H]2CCNC2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	1204	1893	114	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1893	114	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1893	114	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1893	114	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1893	114	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1893	114	None	1	25	Guinea pig	8.2	pKi	=	8.2	Binding	Binding affinity to guinea pig histamine H4 receptorBinding affinity to guinea pig histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1278	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1278	2110	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	1279	2110	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	4908365	2110	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	CHEMBL129198	2110	79	None	3	8	Rat	8.2	pKi	=	8.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.10.104
	44538833	69361	21	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@H]2CCCC[C@H]2O1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935450	69361	21	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@H]2CCCC[C@H]2O1	10.1016/j.bmcl.2011.10.104
	49841719	69379	0	None	38	3	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935571	69379	0	None	38	3	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	309	2	1	6	2.2	CN1CCN(c2nc(N)nc3cc(-c4ccoc4)ccc23)CC1	10.1016/j.bmcl.2011.10.104
	11846300	79841	3	None	-2	4	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL213912	79841	3	None	-2	4	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	327	0	0	4	3.8	CN1CCN(C2=Nc3ccc(Cl)cc3Oc3ccccc32)CC1	10.1016/j.bmcl.2011.10.104
	11301441	95694	6	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260549	95694	6	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	312	1	1	4	2.0	CN1CCN(c2nc3cc(Cl)c(Cl)cc3[nH]c2=O)CC1	10.1016/j.bmcl.2011.10.104
	54582865	61175	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770959	61175	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(N)nc(NC3CCCC3)n2)C1	10.1016/j.bmcl.2011.03.017
	54584825	61206	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770990	61206	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2011.03.017
	25130912	188143	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	CHEMBL505061	188143	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	2	1	6	0.4	CN1CCN(c2cc(N3CCCC3)nc(N)n2)CC1	10.1016/j.bmcl.2011.03.017
	54584825	61206	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL1770990	61206	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	250	4	3	6	0.7	CN[C@@H]1CCN(c2cc(N)nc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	58227157	115404	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354815	115404	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.10.013
	58227200	116606	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393535	116606	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	286	4	2	4	2.5	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	58227337	116624	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393553	116624	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	4	2	5	1.6	CN[C@@H]1CCN(c2cnnc(NC3CCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	24745335	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	8985	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	CHEMBL1915540	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	DB15027	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human H4R expressed in HEK293T cellsDisplacement of [3H]-histamine from human H4R expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1016/j.bmcl.2011.07.125
	24745335	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	8985	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	CHEMBL1915540	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	DB15027	292	7	None	4	5	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	10.1021/acs.jmedchem.5b00516
	11957573	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1250	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	3692	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL19439	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	44595856	89213	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375757	89213	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	356	6	4	8	2.3	Nc1nccc(NCCNc2cc(-c3cccc(Cl)c3)nc(N)n2)n1	10.1021/jm301886t
	1278	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	1279	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	4908365	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	CHEMBL129198	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/jm301886t
	11957573	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	1250	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	3692	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	CHEMBL19439	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1021/jm201042n
	25129523	186694	40	None	1	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL492884	186694	40	None	1	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1016/j.bmcl.2011.06.123
	1278	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	1279	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	4908365	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	CHEMBL129198	2110	79	None	-3	8	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.ejmech.2012.06.016
	11066922	19210	1	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL129436	19210	1	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	311	1	1	2	2.9	CN1CCN(C(=O)c2cc3c(Cl)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573458	75297	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047447	75297	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3c(F)c(Cl)ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573459	75298	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047448	75298	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	295	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	9860842	75299	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)cc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047449	75299	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	1	1	2	2.5	Cc1cc(Cl)cc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1016/j.ejmech.2012.06.016
	11957573	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	1250	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	3692	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	CHEMBL19439	1971	29	None	-40	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	10.1016/j.bmc.2009.04.007
	71525681	89284	0	None	2	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376803	89284	0	None	2	6	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	298	3	2	6	1.7	CNC1CN(c2nc(N)nc3cc(C4CCCC4)cnc23)C1	10.1016/j.bmcl.2013.02.091
	136042270	69557	0	None	426	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938979	69557	0	None	426	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	269	1	2	3	1.4	CN1C[C@H]2CN(C(=N)c3nc4ccccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	1204	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	1247	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	1375	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	774	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL90	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	DB05381	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.04.017
	25178552	172829	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452848	172829	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178552	172829	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL452848	172829	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	387	5	1	6	3.8	CN1CCN(c2nc(NCCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	90645158	111680	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(Cl)cc1-c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290585	111680	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	334	3	1	7	1.5	COc1ccc(Cl)cc1-c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2014.06.032
	57399828	67970	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	345	5	1	5	3.8	CC(C)(C)CCNc1cc(N2CCN(C3CCCCC3)CC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916502	67970	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	345	5	1	5	3.8	CC(C)(C)CCNc1cc(N2CCN(C3CCCCC3)CC2)ncn1	10.1016/j.bmcl.2011.07.114
	805994	173773	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL455133	173773	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm800876b
	805994	173773	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL455133	173773	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	323	4	1	6	2.6	CN1CCN(c2nc(NCc3ccco3)c3ccccc3n2)CC1	10.1021/jm901379s
	168286141	192312	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5192993	192312	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222231	192312	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)cc1	10.1021/acs.jmedchem.1c00692
	46890167	7163	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN1CCC(N(C)CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085872	7163	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	454	7	1	4	5.6	CN1CCC(N(C)CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)C1	10.1016/j.bmcl.2010.04.017
	9966927	18672	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128252	18672	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	9966927	18672	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128252	18672	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0341047
	9966927	18672	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128252	18672	4	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cccc(Br)c3[nH]2)CC1	10.1021/jm0502081
	12686479	161234	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1021/jm7014217
	CHEMBL414183	161234	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1021/jm7014217
	1265	927	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	6422124	927	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	CHEMBL14638	927	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 minsDisplacement of [3H]histamine from human full-length histamine H4 receptor expressed in HEK293 cells after 60 mins	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmc.2011.03.046
	1265	927	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	6422124	927	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	CHEMBL14638	927	54	None	-2290	8	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	10.1016/j.bmcl.2009.10.005
	57390262	67828	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	290	1	2	7	1.2	Nc1nc(N2CC(N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915050	67828	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	290	1	2	7	1.2	Nc1nc(N2CC(N)C2)c2oc3ncc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	155552102	175979	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4576393	175979	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4597352	175979	0	None	9	2	Human	6.2	pKi	=	6.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	3	3	5	1.0	CC(C)(C)CNc1nccc(N2CCN(C(=N)N)CC2)n1	10.1021/acs.jmedchem.9b01342
	25178936	186190	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL489060	186190	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25178936	186190	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL489060	186190	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	305	2	0	5	2.1	CN1CCN(c2nc(N(C)C)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	137646103	157352	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	393	8	3	3	3.7	O=C(O)C(Cc1cccc2ccccc12)NC(=O)[C@@H](CS)Cc1ccccc1	10.1016/j.ejmech.2016.09.074
	CHEMBL4084275	157352	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Antagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting methodAntagonist activity at H4 receptor in human SH-SY5Y cells assessed as inhibition of imetit-induced GTPgamma[35S] binding after 30 mins by microbeta scintillation counting method	ChEMBL	393	8	3	3	3.7	O=C(O)C(Cc1cccc2ccccc12)NC(=O)[C@@H](CS)Cc1ccccc1	10.1016/j.ejmech.2016.09.074
	42624149	109638	1	None	-3	3	Mouse	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236568	109638	1	None	-3	3	Mouse	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from mouse histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	221	3	2	5	0.4	CCc1cc(N2CC[C@@H](NC)C2)nc(N)n1	10.1021/jm401727m
	58227127	116614	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393543	116614	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2ccnc(NC3CCCC3)n2)CC1	10.1016/j.bmcl.2014.12.027
	1204	1893	114	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-12	25	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	44395308	66479	0	None	39	2	Human	7.2	pKi	=	7.2	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	369	1	1	2	2.2	CN1CCN(C(=O)c2cc3cc(I)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	CHEMBL186108	66479	0	None	39	2	Human	7.2	pKi	=	7.2	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	369	1	1	2	2.2	CN1CCN(C(=O)c2cc3cc(I)ccc3[nH]2)CC1	10.1016/j.bmcl.2004.08.035
	155552061	175894	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4543119	175894	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596606	175894	0	None	38	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	277	3	1	5	2.1	CN1CCCN(c2ccnc(NCC(C)(C)C)n2)CC1	10.1021/acs.jmedchem.9b01342
	1204	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from human recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-UR-PI294 from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19 measured after 60 mins by microbeta scintillation counting method	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes co-expressing RGS19	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10017821	103297	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	CHEMBL308913	103297	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	302	8	3	2	2.4	S=C(NCCCCc1c[nH]cn1)NCCc1ccccc1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R expressed in HEK293T cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenatesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell homogenates	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1204	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1247	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1375	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	774	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL90	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	DB05381	1893	114	None	-3	25	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranesDisplacement of [3H]-histamine from human recombinant H4R stably expressed in human SK-N-MC cell membranes	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.0c00160
	10023327	168439	0	None	-144	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL43934	168439	0	None	-144	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor V3.40A mutant expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	390	7	2	3	5.2	Clc1ccc(CN/C(=N\C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	11499454	74056	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	6	2	2	2.5	CC(C)(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL202638	74056	0	None	-1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	6	2	2	2.5	CC(C)(C)NCCCCCc1c[nH]cn1	10.1021/jm0504353
	10446240	74634	1	None	-50	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	277	8	2	2	3.6	Clc1ccc(CNCCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	CHEMBL203263	74634	1	None	-50	3	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	277	8	2	2	3.6	Clc1ccc(CNCCCCCc2c[nH]cn2)cc1	10.1021/jm0504353
	11622495	76209	0	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206303	76209	0	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	8	2	2	2.1	CCCCNCCCCc1c[nH]cn1	10.1021/jm0504353
	1267	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	3035905	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	CHEMBL260374	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.ejmech.2014.03.014
	12686479	161234	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL414183	161234	2	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	244	1	1	5	1.1	CN1CCN(c2nc3ccccc3nc2O)CC1	10.1016/j.bmcl.2011.06.123
	25129523	186694	40	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL492884	186694	40	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor E182'5.46Q mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1039/C2MD20212C
	25129527	186821	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL493720	186821	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from human histamine H4 receptor L175'5.39V mutant transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	303	2	1	5	2.1	CN1CCN(c2cc(-c3ccc(Cl)cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	70681962	75317	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.3	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCNCC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047467	75317	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	277	1	2	2	2.3	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCNCC1	10.1016/j.ejmech.2012.06.016
	70688339	75318	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047468	75318	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	309	1	1	2	2.7	CN1CCCN(C(=O)c2cc3cc(Cl)cc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	1267	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	3035905	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	CHEMBL260374	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm1013488
	1267	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	1267	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	3035905	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	3035905	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	CHEMBL260374	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm7014217
	CHEMBL260374	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/jm901379s
	24854635	95882	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	310	1	0	4	3.0	Cc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	CHEMBL261519	95882	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	310	1	0	4	3.0	Cc1nc2cc(Cl)c(Cl)cc2nc1N1CCN(C)CC1	10.1021/jm7014217
	10085528	193514	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL1202330	193514	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL550570	193514	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	288	7	3	3	2.8	N=C(NCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	10041484	193570	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	316	9	3	3	3.6	N=C(NCCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	CHEMBL551047	193570	0	None	-3	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in human SK-N-MC cells by liquid scintillation counting	ChEMBL	316	9	3	3	3.6	N=C(NCCCCc1ccccc1)SCCCc1c[nH]cn1	10.1016/j.bmc.2009.04.007
	1267	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-33	28	Human	7.2	pKi	=	7.2	Binding	Displacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assayDisplacement of clobenpropit-BODIPY-630/650 from recombinant human NLuc-fused H4R expressed in HEK293T cells by Nano-BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	1252	1978	16	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	9793868	1978	16	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL417096	1978	16	None	-100	4	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting methodDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes measured after 60 mins by microbeta scintillation counting method	ChEMBL	153	5	2	2	1.1	NCCCCCc1cnc[nH]1	10.1021/acs.jmedchem.0c00160
	71681641	89220	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	350	8	4	8	2.2	CC(C)CCc1cc(NCCNc2cc(Cl)nc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375764	89220	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	350	8	4	8	2.2	CC(C)CCc1cc(NCCNc2cc(Cl)nc(N)n2)nc(N)n1	10.1021/jm301886t
	11492189	75638	0	None	-10	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	1	2	2.5	CCN(CC)CCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205511	75638	0	None	-10	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	209	8	1	2	2.5	CCN(CC)CCCCCc1c[nH]cn1	10.1021/jm0504353
	14712103	61541	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	201	5	2	2	1.7	c1ccc(CNCCc2c[nH]cn2)cc1	10.1016/j.bmcl.2011.06.123
	CHEMBL17740	61541	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	201	5	2	2	1.7	c1ccc(CNCCc2c[nH]cn2)cc1	10.1016/j.bmcl.2011.06.123
	12741697	95700	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL260565	95700	2	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in HEK293 cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	0	3	2.6	CN1CCN(c2ccc3cc(Cl)ccc3n2)CC1	10.1016/j.bmcl.2011.06.123
	9793921	64782	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	164	2	1	1	2.5	c1nc(CC2CCCCC2)c[nH]1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824228	64782	7	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	164	2	1	1	2.5	c1nc(CC2CCCCC2)c[nH]1	10.1016/j.bmcl.2011.06.123
	24764570	75294	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	2	1.9	CN[C@@H]1CCN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047444	75294	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	257	2	2	2	1.9	CN[C@@H]1CCN(C(=O)c2cc3cccc(C)c3[nH]2)C1	10.1016/j.ejmech.2012.06.016
	25178938	186167	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm901379s
	CHEMBL488885	186167	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm901379s
	933006	136037	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2c(c1)[nH]c1c(N3CCN(C)CC3)ncnc12	10.1039/C5MD00022J
	CHEMBL3739430	136037	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	297	2	1	5	1.9	COc1ccc2c(c1)[nH]c1c(N3CCN(C)CC3)ncnc12	10.1039/C5MD00022J
	10419556	5271	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106913	5271	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	168273549	192191	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5174342	192191	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221438	192191	0	None	-5	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2ccc(-c3sc(N)nc3C)cc2)cc1	10.1021/acs.jmedchem.1c00692
	66572103	75268	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cnccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047261	75268	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	244	1	1	3	1.0	CN1CCN(C(=O)c2cc3cnccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	164627020	185816	0	None	-144	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	422	7	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4876025	185816	0	None	-144	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	422	7	4	4	3.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)c1ccccc1	10.1016/j.ejmech.2021.113190
	10515993	5869	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079918	5869	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL154944	5869	1	None	-27	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	267	9	2	3	3.0	CCCCC[C@@H](C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	54587792	61210	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.2	Nc1nc(N2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770994	61210	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.2	Nc1nc(N2CCCC2)cc(N2CC[C@@H](N)C2)n1	10.1016/j.bmcl.2011.03.017
	90645156	111678	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	313	3	1	7	0.9	CN1CCN(c2nc(N)nc(-c3ccc(N(C)C)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290583	111678	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	313	3	1	7	0.9	CN1CCN(c2nc(N)nc(-c3ccc(N(C)C)cc3)n2)CC1	10.1016/j.ejmech.2014.06.032
	763018	136198	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	6	1.3	Cc1nnc2c(N3CCN(C)CC3)nc3ccccc3n12	10.1039/C5MD00022J
	CHEMBL3740846	136198	10	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	282	1	0	6	1.3	Cc1nnc2c(N3CCN(C)CC3)nc3ccccc3n12	10.1039/C5MD00022J
	44414498	79571	0	None	-173	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	7	0	2	6.9	CN(Cc1ccc(-c2ccc(CN(C)C3CCCCC3)cc2)cc1)C1CCCCC1	10.1016/j.bmc.2008.10.029
	CHEMBL212775	79571	0	None	-173	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	404	7	0	2	6.9	CN(Cc1ccc(-c2ccc(CN(C)C3CCCCC3)cc2)cc1)C1CCCCC1	10.1016/j.bmc.2008.10.029
	44414433	138986	0	None	-8	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	352	13	2	2	5.9	CCCCCNCc1ccc(-c2ccc(CNCCCCC)cc2)cc1	10.1016/j.bmc.2008.10.029
	CHEMBL379545	138986	0	None	-8	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]RAMHA from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	352	13	2	2	5.9	CCCCCNCc1ccc(-c2ccc(CNCCCCC)cc2)cc1	10.1016/j.bmc.2008.10.029
	10419556	5271	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106913	5271	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	164623155	184983	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	352	3	4	4	3.4	N/C(=N\C(=O)Nc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4863393	184983	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	352	3	4	4	3.4	N/C(=N\C(=O)Nc1ccccc1)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	57395260	69371	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2nc(N)nc(N3CCN(C)CC3)c2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935563	69371	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1ccc2nc(N)nc(N3CCN(C)CC3)c2c1	10.1016/j.bmcl.2011.10.104
	11408069	83834	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	CHEMBL221211	83834	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2010.04.017
	11408069	83834	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL221211	83834	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)cc2Cl)CC1	10.1016/j.bmcl.2006.08.117
	24825995	67788	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914795	67788	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	377	2	2	6	3.0	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Br)cc23)C1	10.1016/j.bmcl.2011.08.014
	1267	3732	43	None	-21	28	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-21	28	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-21	28	Mouse	7.2	pKi	=	7.2	Binding	Inhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to mouse recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	762810	198215	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	262	2	0	5	2.0	CCc1cc2c(N3CCN(C)CC3)ncnc2s1	10.1016/j.bmc.2009.08.059
	CHEMBL594730	198215	11	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	262	2	0	5	2.0	CCc1cc2c(N3CCN(C)CC3)ncnc2s1	10.1016/j.bmc.2009.08.059
	51354299	58565	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	247	8	1	4	2.4	CCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	CHEMBL1688945	58565	0	None	1	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	247	8	1	4	2.4	CCCCCc1cn(CCCc2c[nH]cn2)nn1	10.1021/jm1013488
	136773074	156811	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	CHEMBL4077914	156811	0	None	-	1	Human	5.2	pKi	=	5.2	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	335	6	2	4	3.6	COc1ccc(/C(=N/[C@H](C)Cc2cnc[nH]2)c2ccccc2)c(O)c1	10.1016/j.ejmech.2016.09.074
	57400473	69369	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935561	69369	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	361	3	1	6	2.8	CC(=O)c1cccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)c1	10.1016/j.bmcl.2011.10.104
	57397301	67523	0	None	-891	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	560	11	1	6	4.6	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910382	67523	0	None	-891	3	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	560	11	1	6	4.6	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4cc(Cl)ccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	127026018	136954	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753985	136954	0	None	-3	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	73346048	91421	0	None	-15	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	7	2	3	3.0	O=C(NCCc1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL2413359	91421	0	None	-15	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	307	7	2	3	3.0	O=C(NCCc1ccc(Cl)cc1)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	164617783	183997	0	None	-104	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	8	4	4	2.8	CCC(CNC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4848815	183997	0	None	-104	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	8	4	4	2.8	CCC(CNC(=O)/N=C(\N)NCCCc1sc(N)nc1C)c1ccccc1	10.1016/j.ejmech.2021.113190
	71519499	86051	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	1	6	2.1	CN1CCN(c2nc(N)nc3c2cc(-c2ccc(F)cc2)n3C)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314768	86051	0	None	-	1	Human	4.2	pKi	=	4.2	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	340	2	1	6	2.1	CN1CCN(c2nc(N)nc3c2cc(-c2ccc(F)cc2)n3C)CC1	10.1016/j.bmcl.2012.10.139
	54583872	61176	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770960	61176	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	298	5	3	6	1.5	CN[C@@H]1CCN(c2cc(N)nc(NCc3ccccc3)n2)C1	10.1016/j.bmcl.2011.03.017
	1278	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	1279	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	4908365	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	CHEMBL129198	2110	79	None	-3	8	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 cells co-expressing mammalian Galphai2 and Gbeta1gamma2	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1021/acs.jmedchem.6b00120
	58227388	116609	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393538	116609	1	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	249	4	1	5	1.3	CC(C)CNc1nccc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2014.12.027
	155517880	176083	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	155517880	176083	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4445423	176083	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4598198	176083	0	None	16	2	Human	7.2	pKi	=	7.2	Binding	Displacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 40 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	291	4	4	5	1.0	CC(C)(C)CNc1nccc(N2CC[C@@H](NC(=N)N)C2)n1	10.1021/acs.jmedchem.9b01342
	46890626	6495	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@@H]3CCCN[C@@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083097	6495	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	422	6	2	4	4.4	Cc1cc(OCCCN2C[C@@H]3CCCN[C@@H]3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	25178938	186167	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm800876b
	CHEMBL488885	186167	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	271	2	1	5	1.7	CNc1nc(N2CCN(C)CC2)nc2cccc(C)c12	10.1021/jm800876b
	9812843	203523	1	None	-4	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	CHEMBL78838	203523	1	None	-4	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	159	2	1	2	1.4	c1cncc(Cc2c[nH]cn2)c1	10.1021/jm049932u
	647957	28849	12	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	2	0	6	0.4	Cc1cc(N2CCN(C)CC2)nc(N2CCOCC2)n1	10.1039/C5MD00022J
	CHEMBL1381010	28849	12	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	277	2	0	6	0.4	Cc1cc(N2CCN(C)CC2)nc(N2CCOCC2)n1	10.1039/C5MD00022J
	747187	136102	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	249	4	1	3	2.8	Cc1cc2nc(SCCN(C)C)[nH]c2cc1C	10.1039/C5MD00022J
	CHEMBL3739949	136102	9	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293T cells after 1 to 1.5 hrs by liquid scintillation counting analysis	ChEMBL	249	4	1	3	2.8	Cc1cc2nc(SCCN(C)C)[nH]c2cc1C	10.1039/C5MD00022J
	168268716	192150	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5170276	192150	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221176	192150	0	None	-3	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	372	3	4	4	3.5	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NC2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	168268853	192173	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5180504	192173	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221353	192173	0	None	-83	6	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	389	8	4	5	1.9	Cc1ccc(CC(C)CNC(=O)/N=C(\N)NCCCc2nnc(N)s2)cc1	10.1021/acs.jmedchem.1c00692
	5013	99760	28	None	-16	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2ccc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL288591	99760	28	None	-16	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	227	1	0	3	2.0	CN1CCN(c2ccc3ccccc3n2)CC1	10.1021/jm7014217
	10243990	5802	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079552	5802	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL155830	5802	0	None	-79	3	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	253	8	2	3	2.6	CCCCC(C)NC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	11065080	19221	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1007/s00044-004-0106-7
	CHEMBL129488	19221	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1007/s00044-004-0106-7
	11065080	19221	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0341047
	CHEMBL129488	19221	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0341047
	117724523	116600	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393529	116600	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	6	2	4	2.1	CCCCNc1cc(N2CC[C@@H](NC)C2)ccn1	10.1016/j.bmcl.2014.12.027
	11594278	14506	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	CHEMBL120591	14506	0	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	276	1	1	3	1.4	Cc1c(F)ccc2[nH]c(C(=O)N3CCN(C)CC3)nc12	10.1021/jm0502081
	11065080	19221	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	CHEMBL129488	19221	2	None	-	1	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	257	1	1	2	1.9	Cc1cccc2cc(C(=O)N3CCN(C)CC3)[nH]c12	10.1021/jm0502081
	44462956	5508	0	None	-2	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	CHEMBL1077276	5508	0	None	-2	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation countingDisplacement of [3H]-histamine from human histamine H4 receptor expressed in HEK293 cells after 1 hr by liquid scintillation counting	ChEMBL	363	2	2	5	3.1	Nc1nc2c(c(N3CC4CCCNC4C3)n1)CCCCC2c1ccccc1	10.1016/j.bmcl.2010.01.131
	71603140	89275	0	None	97	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	3	2	5	1.5	CNC1CN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376794	89275	0	None	97	2	Human	8.2	pKi	=	8.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	269	3	2	5	1.5	CNC1CN(c2nc(N)nc3cc(C4CC4)ccc23)C1	10.1016/j.bmcl.2013.02.091
	25147772	172827	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452847	172827	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	25147772	172827	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL452847	172827	4	None	-1	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	373	4	1	6	3.7	CN1CCN(c2nc(NCc3cccs3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	44129131	197763	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	440	8	1	7	2.1	CCN(CC)S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL591500	197763	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	440	8	1	7	2.1	CCN(CC)S(=O)(=O)CCNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	25071319	188464	8	None	9	2	Rat	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188464	8	None	9	2	Rat	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	16082828	79835	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	CHEMBL213886	79835	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	275	6	2	2	3.3	Clc1ccc(CNCC[C@@H]2C[C@H]2c2c[nH]cn2)cc1	10.1021/jm0603318
	57398091	67751	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	3.0	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914757	67751	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	1	1	6	3.0	CN1CCN(c2nc(N)nc3c2oc2ccc(Cl)c(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	57401517	67768	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)c(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914774	67768	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	337	1	2	6	2.7	Nc1nc(N2CCNCC2)c2oc3ccc(Cl)c(Cl)c3c2n1	10.1016/j.bmcl.2011.08.014
	3035746	187587	6	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL1237146	187587	6	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	CHEMBL498770	187587	6	None	-1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	414	7	2	3	3.2	N/C(=N/CCc1ccc(I)cc1)SCCCc1cnc[nH]1	10.1021/jm7014777
	136030655	56173	0	None	2	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632413	56173	0	None	2	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	1	2	3	2.2	CN1CCN(/C(=N\O)c2cc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	136192874	56174	0	None	1	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632414	56174	0	None	1	3	Mouse	8.1	pKi	=	8.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	292	2	2	4	2.7	CN1CCC(/C(=N/O)c2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2010.10.041
	1204	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1247	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1375	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	774	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2004.08.035
	1204	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	53321919	56169	0	None	-3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL1632409	56169	0	None	-3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	322	7	3	3	3.8	N=C(NCc1ccc(Cl)cc1)SCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	10060213	102425	1	None	3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	CHEMBL306077	102425	1	None	3	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	240	6	3	2	2.0	CC(C)/N=C(\S)NCCCCc1c[nH]cn1	10.1016/j.bmcl.2010.10.041
	155491015	175911	3	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4441731	175911	3	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	CHEMBL4596734	175911	3	None	-7	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysisDisplacement of [3H]NAMH from human H4R expressed in HEK293T cells incubated for 2 hrs by microbeta scintillation counting analysis	ChEMBL	207	4	2	5	0.2	CCCNC1CN(c2ccnc(N)n2)C1	10.1021/acs.jmedchem.9b01462
	44609412	89230	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	322	6	4	8	1.6	Nc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375773	89230	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	322	6	4	8	1.6	Nc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	25129523	186694	40	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm301886t
	CHEMBL492884	186694	40	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 minsDisplacement of [3H]histamine displacement from human recombinant histamine H4 receptor expressed in SK-NM-C cells after 45 mins	ChEMBL	269	2	1	5	1.5	CN1CCN(c2cc(-c3ccccc3)nc(N)n2)CC1	10.1021/jm301886t
	51354584	58577	0	None	11	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	6	1	4	2.4	c1nc(CCCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688976	58577	0	None	11	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	6	1	4	2.4	c1nc(CCCn2cc(CC3CCCC3)nn2)c[nH]1	10.1021/jm1013488
	76314798	105559	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	314	3	1	7	1.4	CN1CCN(c2cc(-c3ccc([N+](=O)[O-])cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	CHEMBL3133131	105559	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	314	3	1	7	1.4	CN1CCN(c2cc(-c3ccc([N+](=O)[O-])cc3)nc(N)n2)CC1	10.1039/C2MD20212C
	136042269	69556	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938978	69556	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	323	1	2	3	1.8	CN1C[C@H]2CN(C(=N)c3nc4c(F)c(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	71525678	89281	0	None	-7	6	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376800	89281	0	None	-7	6	Rat	7.2	pKi	=	7.2	Binding	Binding affinity to rat histamine H4 receptorBinding affinity to rat histamine H4 receptor	ChEMBL	258	3	2	6	0.6	CCc1cnc2c(N3CC(NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25178937	186192	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL489062	186192	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25071319	188464	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188464	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	44129209	200335	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	586	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccc(I)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL608373	200335	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	586	7	2	7	3.5	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccc(I)cc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	11013044	18707	1	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	333	4	1	2	3.2	O=C(c1cc2ccccc2[nH]1)N1CCN(CCc2ccccc2)CC1	10.1021/jm0341047
	CHEMBL128470	18707	1	None	-	1	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	333	4	1	2	3.2	O=C(c1cc2ccccc2[nH]1)N1CCN(CCc2ccccc2)CC1	10.1021/jm0341047
	122194625	123490	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	CHEMBL3629828	123490	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	310	3	1	6	1.7	Cc1cccc(/C=C/c2nc(N)nc(N3CCN(C)CC3)n2)c1	10.1016/j.ejmech.2015.08.014
	52952957	62818	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1774594	62818	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	CHEMBL1789799	62818	0	None	-29	2	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2 subunit after 60 mins by liquid scintillation counting	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmc.2011.03.046
	24854703	166323	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	364	5	0	6	3.0	COc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	CHEMBL428606	166323	0	None	-	1	Human	6.2	pKi	=	6.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	364	5	0	6	3.0	COc1ccc(COc2nc3ccccc3nc2N2CCN(C)CC2)cc1	10.1021/jm7014217
	156013005	176915	0	None	-2	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	899	20	2	7	10.6	Cc1cc(OCCCNC(=O)CCCCCN2/C(=C\C=C\C=C\C3=[N+](CCCCS(=O)(=O)[O-])c4ccccc4C3(C)C)C(C)(C)c3ccccc32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	CHEMBL4639472	176915	0	None	-2	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	899	20	2	7	10.6	Cc1cc(OCCCNC(=O)CCCCCN2/C(=C\C=C\C=C\C3=[N+](CCCCS(=O)(=O)[O-])c4ccccc4C3(C)C)C(C)(C)c3ccccc32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1021/acs.jmedchem.0c00160
	168268827	192167	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5179906	192167	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221314	192167	0	None	-6	4	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	450	8	4	4	4.8	Cc1nc(N)sc1-c1cccc(N/C(N)=N/C(=O)NCC(C)CCCc2ccccc2)c1	10.1021/acs.jmedchem.1c00692
	52952957	62818	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1774594	62818	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	CHEMBL1789799	62818	0	None	-29	2	Human	5.2	pKi	=	5.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunitDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Gai2 and Gb1c2 subunit	ChEMBL	251	8	2	3	2.4	CCC/C=C/CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2013.06.075
	25071319	188464	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	CHEMBL509540	188464	8	None	-9	2	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1021/jm800670r
	46228101	198106	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL594022	198106	0	None	-	1	Human	7.2	pKi	=	7.2	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	332	4	2	6	2.1	CN1CCN(c2cc(NCc3ccc(Cl)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46228083	198049	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	374	5	2	6	3.1	CN1CCN(c2cc(NCc3ccc(-c4ccccc4)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL593551	198049	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	374	5	2	6	3.1	CN1CCN(c2cc(NCc3ccc(-c4ccccc4)cc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	46890549	6492	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083085	6492	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	396	6	2	4	4.2	Cc1cc(OCCCN2CCCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	112936352	136956	1	None	-17	2	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2cc(-c3ccccc3)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	CHEMBL3754008	136956	1	None	-17	2	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	389	6	1	6	3.5	COc1ccc(CNc2cc(-c3ccccc3)nc(N3CCN(C)CC3)n2)cc1	10.1016/j.bmcl.2015.12.035
	155563489	174747	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.7	CCC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4573929	174747	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	250	3	1	6	0.7	CCC(C)c1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	71518865	86021	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	333	2	2	6	1.8	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(C#N)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314494	86021	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	333	2	2	6	1.8	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(C#N)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	57401167	69511	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938846	69511	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	306	1	1	3	1.5	CN1C[C@H]2CN(C(=O)c3nc4c(F)c(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24826679	67745	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914751	67745	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	1	6	2.4	CN1CCN(c2nc(N)nc3c2oc2cccc(Cl)c23)CC1	10.1016/j.bmcl.2011.08.014
	168294161	192394	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5204739	192394	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222742	192394	0	None	-7	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1cccc(-c2sc(N)nc2C)c1	10.1021/acs.jmedchem.1c00692
	168294770	192408	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208327	192408	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222806	192408	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	436	6	4	4	4.3	Cc1ccc(CC(C)CNC(=O)/N=C(\N)Nc2cccc(-c3sc(N)nc3C)c2)cc1	10.1021/acs.jmedchem.1c00692
	44450438	95662	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc3ccccc3nc2NCc2ccccc2)CC1	10.1021/jm7014217
	CHEMBL260371	95662	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	333	4	1	5	3.0	CN1CCN(c2nc3ccccc3nc2NCc2ccccc2)CC1	10.1021/jm7014217
	71528209	86530	0	None	-1148	7	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	465	6	0	3	5.8	CC1=CC(C)=[N+]2C1=C(c1ccc(OCCCN3CCCCC3)cc1)c1c(C)cc(C)n1[B-]2(F)F	10.1021/ml300383n
	CHEMBL2323582	86530	0	None	-1148	7	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells	ChEMBL	465	6	0	3	5.8	CC1=CC(C)=[N+]2C1=C(c1ccc(OCCCN3CCCCC3)cc1)c1c(C)cc(C)n1[B-]2(F)F	10.1021/ml300383n
	121238069	158568	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting methodDisplacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.04.033
	CHEMBL4097856	158568	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting methodDisplacement of [3H]-histamine from human histamine H4R expressed in insect Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.04.033
	24826268	67694	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	3	2	6	2.8	CCN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914544	67694	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	3	2	6	2.8	CCN[C@@H]1CCN(c2nc(N)nc3c2oc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	56851443	71269	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1923024	71269	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	CHEMBL1963459	71269	0	None	-7	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	356	7	2	3	4.6	c1ccc(CN/C(=N/C2CCCCC2)SCCCc2c[nH]cn2)cc1	10.1021/jm201042n
	121238069	158568	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	CHEMBL4097856	158568	0	None	-79	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	284	3	1	6	0.8	CN1CCN(c2nc(N)nc(Cc3ccccc3)n2)CC1	10.1016/j.ejmech.2017.09.035
	25178132	186346	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL490073	186346	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25178132	186346	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL490073	186346	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	2.9	CC(C)Nc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	168283321	192283	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5183015	192283	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222057	192283	0	None	-6	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	364	7	4	4	2.3	Cc1nc(N)oc1CCCN/C(N)=N/C(=O)NCC(C)C1CCCCC1	10.1021/acs.jmedchem.1c00692
	24759622	169101	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL443764	169101	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm800876b
	24759622	169101	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm7014217
	24759622	169101	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL443764	169101	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL443764	169101	1	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2ncc3ccccc3n2)CC1	10.1021/jm901379s
	164622259	185138	0	None	-7244	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	336	6	4	5	1.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccco1	10.1016/j.ejmech.2021.113190
	CHEMBL4865965	185138	0	None	-7244	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	336	6	4	5	1.4	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCc1ccco1	10.1016/j.ejmech.2021.113190
	24826167	67789	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914796	67789	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	333	2	2	6	2.9	CN[C@H]1CCN(c2nc(N)nc3c2sc2ccc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	54581938	61188	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770972	61188	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	261	3	2	5	1.1	CN[C@@H]1CCN(c2cc(N)nc(N3CCCC3)c2)C1	10.1016/j.bmcl.2011.03.017
	46890019	7216	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086085	7216	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2C3CN(C)CC32)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	24994945	109637	1	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CN[C@@H]1CCN(c2cc(C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236567	109637	1	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	207	2	2	5	0.2	CN[C@@H]1CCN(c2cc(C)nc(N)n2)C1	10.1021/jm401727m
	1223	938	32	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	2790	938	32	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	CHEMBL14690	938	32	None	-60	11	Human	7.1	pKi	=	7.1	Binding	Inhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assayInhibition of UR-DEBa242 binding to human recombinant NLuc/GPCR-fused H4R expressed in HEK293T cells measured after 30 mins by furimazine substrate based BRET assay	ChEMBL	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	10.1021/acs.jmedchem.0c00160
	51354301	58569	0	None	-10	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688969	58569	0	None	-10	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.4	c1nc(CCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	164609017	183833	0	None	-13	6	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	10	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCCc1ccccc1	10.1016/j.ejmech.2021.113190
	CHEMBL4846201	183833	0	None	-13	6	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	416	10	4	4	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NCC(C)CCCc1ccccc1	10.1016/j.ejmech.2021.113190
	168294166	192402	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5209081	192402	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222775	192402	0	None	-4	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	360	6	4	4	3.3	CCCCCNC(=O)/N=C(\N)Nc1ccc(-c2sc(N)nc2C)cc1	10.1021/acs.jmedchem.1c00692
	168294178	192423	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5205197	192423	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222887	192423	0	None	-5	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	414	5	4	4	4.4	Cc1nc(N)sc1-c1ccc(N/C(N)=N/C(=O)NCC(C)C2CCCCC2)cc1	10.1021/acs.jmedchem.1c00692
	127026019	136922	0	None	-6	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	378	2	0	6	1.7	CN1CCN(c2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753705	136922	0	None	-6	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	378	2	0	6	1.7	CN1CCN(c2nc3c(c(N4CCN(C)CC4)n2)-c2ccccc2CC3)CC1	10.1016/j.bmcl.2015.12.035
	11546127	95027	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	CHEMBL25725	95027	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Agonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assayAgonist activity at human H4 receptor expressed in CHO cell membranes co-expressing Galphai2 by GTPgamma[35S] binding assay	ChEMBL	414	7	2	5	3.3	COc1ccc2c(c1)c(CC(=O)NCCCO)c(C)n2C(=O)c1ccc(Cl)cc1	10.1016/j.ejmech.2016.09.074
	10715823	5838	0	None	-20	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	281	10	2	3	3.3	CCCCC(CC)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	CHEMBL1079747	5838	0	None	-20	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in SF9 cells coexpressing Galphai2, Gbeta1gamma2	ChEMBL	281	10	2	3	3.3	CCCCC(CC)CNC(=O)OCCCc1c[nH]cn1	10.1016/j.bmcl.2009.10.005
	1267	3732	43	None	-33	28	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	3035905	3732	43	None	-33	28	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	CHEMBL260374	3732	43	None	-33	28	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1016/j.bmcl.2011.10.104
	25071319	188464	8	None	-9	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.06.123
	CHEMBL509540	188464	8	None	-9	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	295	1	2	5	1.6	Nc1nc2c(c(N3CCNCC3)n1)CCCc1ccccc1-2	10.1016/j.bmcl.2011.06.123
	25109291	190782	9	None	-501	8	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1016/j.bmcl.2011.06.123
	CHEMBL519240	190782	9	None	-501	8	Human	7.1	pKi	=	7.1	Binding	Binding affinity to H4R receptorBinding affinity to H4R receptor	ChEMBL	327	1	2	6	1.7	Nc1nc2c(c(N3CCNCC3)n1)CCC1=C2[C@@H]2CCCC[C@@H]2O1	10.1016/j.bmcl.2011.06.123
	1204	1893	114	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1247	1893	114	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	1375	1893	114	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	774	1893	114	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL90	1893	114	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	DB05381	1893	114	None	-15	25	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2010.10.041
	10446295	2092	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	1277	2092	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	CHEMBL185951	2092	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	10.1016/j.bmcl.2004.08.035
	1267	3732	43	None	-21	28	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	3035905	3732	43	None	-21	28	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	CHEMBL260374	3732	43	None	-21	28	Mouse	7.1	pKi	=	7.1	Binding	Displacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cellsDisplacement of UR-DEBa176 from mouse recombinant H4R expressed in HEK293T-SF-His6-CRE-Luc cells	ChEMBL	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	10.1021/acs.jmedchem.0c00160
	937120	174519	8	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	244	1	0	3	1.8	CN1CCN(C(=O)c2cc3ccccc3o2)CC1	10.1016/j.bmc.2020.115924
	CHEMBL4568949	174519	8	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	244	1	0	3	1.8	CN1CCN(C(=O)c2cc3ccccc3o2)CC1	10.1016/j.bmc.2020.115924
	71681974	89205	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1cc(-c2ccccc2)nc(N)n1)c1ccnc(N)n1	10.1021/jm301886t
	CHEMBL2375749	89205	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	336	6	3	8	1.6	CN(CCNc1cc(-c2ccccc2)nc(N)n1)c1ccnc(N)n1	10.1021/jm301886t
	71681475	89211	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	317	8	5	9	1.0	CC(C)CNc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	CHEMBL2375755	89211	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	317	8	5	9	1.0	CC(C)CNc1cc(NCCNc2ccnc(N)n2)nc(N)n1	10.1021/jm301886t
	71681643	89222	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375766	89222	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	378	9	4	8	3.1	CC(C)CNc1nccc(NCCNc2cc(-c3ccccc3)nc(N)n2)n1	10.1021/jm301886t
	71681645	89224	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375768	89224	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	302	8	4	8	1.4	CC(C)CNc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	11679993	75649	0	None	-25	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.0	CC(C)CNCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL205559	75649	0	None	-25	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	7	2	2	2.0	CC(C)CNCCCCc1c[nH]cn1	10.1021/jm0504353
	11087453	75277	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047270	75277	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cc([N+](=O)[O-])ccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66572113	75282	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3ccc(N)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047275	75282	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3ccc(N)cc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	66573457	75296	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	2	1	2	2.4	Cc1cccc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	CHEMBL2047446	75296	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	283	2	1	2	2.4	Cc1cccc2cc(C(=O)N3CC(N4CCCC4)C3)[nH]c12	10.1016/j.ejmech.2012.06.016
	70688340	75326	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3c(F)ccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047476	75326	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	279	1	1	2	1.8	CN1CCN(C(=O)c2cc3c(F)ccc(F)c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	51354875	58585	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688986	58585	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCc2cn(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25178937	186192	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL489062	186192	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	11846635	78255	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	CHEMBL211242	78255	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cellsDisplacement of [3H]histamine from human histamine H4 receptor transfected in SK-N-MC cells	ChEMBL	307	0	0	4	3.4	Cc1ccc2c(c1)Oc1ccccc1C(N1CCN(C)CC1)=N2	10.1021/jm051008s
	4060730	19168	51	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	CHEMBL129199	19168	51	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysisDisplacement of [3H]histamine from wild type human histamine H4 receptor transfected in HEK293T cells after 1.5 hrs by liquid scintillation counting analysis	ChEMBL	243	1	1	2	1.6	CN1CCN(C(=O)c2cc3ccccc3[nH]2)CC1	10.1039/C2MD20212C
	90645159	111681	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	2.2	CN1CCN(c2nc(N)nc(-c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	CHEMBL3290586	111681	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	338	2	1	6	2.2	CN1CCN(c2nc(N)nc(-c3ccc(Cl)c(Cl)c3)n2)CC1	10.1016/j.ejmech.2014.06.032
	162671115	182355	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	266	2	0	3	1.9	CN1CCN(CC2Cc3cc(Cl)ccc3O2)CC1	10.1016/j.bmc.2020.115924
	CHEMBL4792753	182355	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysisDisplacement of [3H]-Histamine from human histamine 4 receptor transfected in HEK293T cells incubated for 16 hrs by liquid scintillation counter analysis	ChEMBL	266	2	0	3	1.9	CN1CCN(CC2Cc3cc(Cl)ccc3O2)CC1	10.1016/j.bmc.2020.115924
	10197870	5214	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL106612	5214	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	91613	17789	31	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	CHEMBL12620	17789	31	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	125	2	2	2	0.2	Cc1ncc(CCN)[nH]1	10.1016/j.bmcl.2010.10.041
	71681474	89210	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	246	5	4	8	-0.0	Nc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	CHEMBL2375754	89210	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 minsDisplacement of [3H]histamine displacement from human histamine H4 receptor expressed in SK-NM-C cells after 60 mins	ChEMBL	246	5	4	8	-0.0	Nc1nccc(NCCNc2ccnc(N)n2)n1	10.1021/jm301886t
	11637064	74590	0	None	-25	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	1	2	2.2	c1nc(CCCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	CHEMBL203228	74590	0	None	-25	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	207	6	1	2	2.2	c1nc(CCCCCN2CCCC2)c[nH]1	10.1021/jm0504353
	11708125	76151	3	None	-251	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	5	1	2	1.3	CN(C)CCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL206000	76151	3	None	-251	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	167	5	1	2	1.3	CN(C)CCCCc1c[nH]cn1	10.1021/jm0504353
	11499376	137961	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	5	2	2	2.1	CC(C)(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	CHEMBL377491	137961	0	None	-1	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human H4 receptor expressed in SK-N-MC cells	ChEMBL	195	5	2	2	2.1	CC(C)(C)NCCCCc1c[nH]cn1	10.1021/jm0504353
	56672434	64778	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	1	2	3.3	CN1CCC(=Cc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2011.06.123
	CHEMBL1824048	64778	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation countingDisplacement of [3H]histamine from human H4R receptor expressed in SK-N-MC cells at 10 uM after 1.5 hrs by liquid scintillation counting	ChEMBL	261	1	1	2	3.3	CN1CCC(=Cc2nc3cc(Cl)ccc3[nH]2)CC1	10.1016/j.bmcl.2011.06.123
	66572109	75274	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3c([N+](=O)[O-])cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047267	75274	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3c([N+](=O)[O-])cccc3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	10957150	75291	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cccc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	CHEMBL2047441	75291	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	288	2	1	4	1.5	CN1CCN(C(=O)c2cc3cccc([N+](=O)[O-])c3[nH]2)CC1	10.1016/j.ejmech.2012.06.016
	3939214	126111	7	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	262	1	1	1	3.4	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCCC1	10.1016/j.bmcl.2004.08.035
	CHEMBL365257	126111	7	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Binding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranesBinding affinity towards human histamine H4 receptor was determined by [3H]Na-methylhistamine binding to SK-N-MC cell membranes	ChEMBL	262	1	1	1	3.4	O=C(c1cc2cc(Cl)ccc2[nH]1)N1CCCCC1	10.1016/j.bmcl.2004.08.035
	11086114	7298	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	CHEMBL108649	7298	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1016/j.bmcl.2010.10.041
	2247	502	77	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	249	502	77	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	2603	502	77	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	CHEMBL296419	502	77	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	DB00637	502	77	None	-117	42	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	10.1016/j.bmcl.2011.09.001
	44417471	12647	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	CHEMBL1187948	12647	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	CHEMBL534496	12647	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	175	4	5	3	-0.5	N=C(N)NCCCSC(=N)N	10.1021/jm060880d
	164614841	184682	0	None	-223	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	7	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	CHEMBL4858849	184682	0	None	-223	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	388	7	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(c1ccccc1)C(C)C	10.1016/j.ejmech.2021.113190
	9923162	18736	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL128613	18736	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1007/s00044-004-0106-7
	10967174	19227	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	CHEMBL129540	19227	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Antagonist activity at Homo sapiens (human) histamine H4 receptorAntagonist activity at Homo sapiens (human) histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1007/s00044-004-0106-7
	1204	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1247	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	1375	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	774	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Binding affinity to histamine H4 receptor (unknown origin)Binding affinity to histamine H4 receptor (unknown origin)	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1021/acs.jmedchem.5b00516
	9923162	18736	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0341047
	CHEMBL128613	18736	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0341047
	10967174	19227	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	CHEMBL129540	19227	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]- histamine from the recombinant human histamine H4 receptorDisplacement of [3H]- histamine from the recombinant human histamine H4 receptor	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0341047
	1278	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	1279	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	4908365	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	CHEMBL129198	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.125
	54580746	61047	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.1	Nc1nc(N2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770160	61047	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	248	2	2	6	0.1	Nc1nc(N2CCCC2)cc(N2CCNCC2)n1	10.1016/j.bmcl.2011.03.017
	1278	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	1279	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	4908365	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	CHEMBL129198	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.07.114
	58227157	115404	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3354815	115404	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	235	4	2	5	1.1	CN[C@@H]1CCN(c2ccnc(NC(C)C)n2)C1	10.1016/j.bmcl.2014.12.027
	58227179	116619	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393548	116619	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@H]1CCN(c2ccnc(NC3CC4CCC3C4)n2)C1	10.1016/j.bmcl.2014.12.027
	58227259	116627	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393556	116627	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	4	2	5	1.9	CN[C@@H]1CCN(c2cnnc(NC3CC4CCC3C4)c2)C1	10.1016/j.bmcl.2014.12.027
	1278	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	1279	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	4908365	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	CHEMBL129198	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	10.1016/j.bmcl.2011.11.098
	42624150	109647	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	CHEMBL3236577	109647	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	249	2	2	5	1.2	CN[C@@H]1CCN(c2cc(C(C)(C)C)nc(N)n2)C1	10.1021/jm401727m
	24827129	67778	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2c(Cl)cc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914785	67778	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	351	2	2	6	3.1	CN[C@@H]1CCN(c2nc(N)nc3c2oc2c(Cl)cc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	9923162	18736	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL128613	18736	5	None	199	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	321	1	1	2	2.3	CN1CCN(C(=O)c2cc3cc(Br)ccc3[nH]2)CC1	10.1021/jm0502081
	10967174	19227	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	CHEMBL129540	19227	3	None	-2	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	258	1	2	3	1.1	CN1CCN(C(=O)c2cc3cccc(N)c3[nH]2)CC1	10.1021/jm0502081
	44129212	197762	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.3	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(C(F)(F)F)cccc3n2)CC1	10.1021/jm901379s
	CHEMBL591496	197762	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	494	7	2	7	3.3	CN1CCN(c2nc(NCCS(=O)(=O)Nc3ccccc3)c3c(C(F)(F)F)cccc3n2)CC1	10.1021/jm901379s
	24825522	187050	0	None	-6	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	283	3	1	3	3.3	CC1CC(C)(C)N=C(c2cccc(Cc3c[nH]cn3)c2)O1	10.1021/jm7014149
	CHEMBL494982	187050	0	None	-6	3	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells	ChEMBL	283	3	1	3	3.3	CC1CC(C)(C)N=C(c2cccc(Cc3c[nH]cn3)c2)O1	10.1021/jm7014149
	51354300	58566	0	None	15	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688946	58566	0	None	15	2	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	273	6	1	4	2.8	c1nc(CCCn2cc(CC3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	58227373	175924	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4455324	175924	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	CHEMBL4596881	175924	0	None	1	2	Human	8.1	pKi	=	8.1	Binding	Displacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assayDisplacement of 10 nM [3H]-histamine from human H4R expressed in Sf9 cell membranes co-expressing Gia2 and beta1gamma2 by competition binding assay	ChEMBL	249	3	2	5	1.5	CC(C)(C)CNc1nccc(N2CC[C@@H](N)C2)n1	10.1021/acs.jmedchem.9b01342
	168294182	192436	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5208722	192436	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	CHEMBL5222960	192436	0	None	-5	4	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	314	6	4	2	1.7	C[C@@H](NC(=O)/N=C(\N)NCCCc1c[nH]cn1)c1ccccc1	10.1021/acs.jmedchem.1c00692
	46890546	6422	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1082771	6422	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	382	6	2	4	3.8	Cc1cc(OCCCN2CCC(N)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46228069	198456	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	3	1	7	1.2	CN1CCN(c2nc(N)nc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL596348	198456	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	323	3	1	7	1.2	CN1CCN(c2nc(N)nc3c2ncn3Cc2ccccc2)CC1	10.1016/j.bmc.2009.08.059
	63868172	175472	2	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	222	2	1	6	-0.2	CCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	CHEMBL4590628	175472	2	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2Displacement of [3H]-histamine from recombinant human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2/Gbeta1gamma2	ChEMBL	222	2	1	6	-0.2	CCc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.bmc.2019.02.020
	164614549	184350	0	None	-575	6	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	340	9	4	4	2.2	CCCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4853668	184350	0	None	-575	6	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	340	9	4	4	2.2	CCCCCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	164621681	184988	0	None	-64	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	444	7	4	5	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(OC(F)(F)F)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4863523	184988	0	None	-64	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	444	7	4	5	3.2	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(OC(F)(F)F)cc1	10.1016/j.ejmech.2021.113190
	164627241	185837	0	None	-269	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	6	4	4	3.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Br)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4876267	185837	0	None	-269	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	438	6	4	4	3.1	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Br)cc1	10.1016/j.ejmech.2021.113190
	11086114	7298	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL108649	7298	0	None	-1	2	Human	5.1	pKi	=	5.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	248	3	3	4	0.3	C/N=C(\NC#N)NC[C@H]1CC[C@@H](c2c[nH]cn2)O1	10.1021/jm0300025
	71519347	86048	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	274	4	2	6	1.2	CCCCn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	CHEMBL2314765	86048	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	274	4	2	6	1.2	CCCCn1ccc2c(N3CCNCC3)nc(N)nc21	10.1016/j.bmcl.2012.10.139
	54754351	67860	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915535	67860	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.125
	54754351	67860	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1915535	67860	0	None	10	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	277	4	1	5	2.1	CN1CCN(c2cc(NCCC(C)(C)C)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	58227242	116623	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2cnnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393552	116623	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	261	3	1	5	1.6	CN1CCN(c2cnnc(NC3CCCC3)c2)CC1	10.1016/j.bmcl.2014.12.027
	121238067	166547	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2017.09.035
	CHEMBL4290148	166547	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]histamine from human H4R expressed in Sf9 cell membranes co-expressed with G protein Gai2 and Gb1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	250	3	1	6	0.4	CC(C)Cc1nc(N)nc(N2CCN(C)CC2)n1	10.1016/j.ejmech.2017.09.035
	57400778	67853	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915346	67853	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.07.125
	54584827	61212	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770996	61212	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	262	3	2	6	0.5	CN[C@H]1CCN(c2cc(N3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	57400778	67853	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915346	67853	0	None	199	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	288	1	1	3	1.3	CN1C[C@H]2CN(C(=O)c3nc4cc(F)ccc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	57400589	69550	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)cnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938972	69550	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	304	1	1	3	1.9	CN1C[C@H]2CN(C(=O)c3cc4cc(Cl)cnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	90645164	111686	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1C	10.1016/j.ejmech.2014.06.032
	CHEMBL3290591	111686	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor expressed in insect Sf9 cell membrane co-expressing Galphai2/Gbeta1gamma2 subunits after 60 mins by liquid scintillation counting analysis	ChEMBL	298	2	1	6	1.5	Cc1ccc(-c2nc(N)nc(N3CCN(C)CC3)n2)cc1C	10.1016/j.ejmech.2014.06.032
	57399016	67819	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915040	67819	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	46890548	6491	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1083084	6491	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	410	7	1	4	4.4	Cc1cc(OCCCN2CCC(N(C)C)C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	46929551	16550	0	None	-52	2	Human	6.1	pKi	=	6.1	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2ccccn2)c2c[nH]cn2)cc1	10.1021/jm100643t
	CHEMBL1243335	16550	0	None	-52	2	Human	6.1	pKi	=	6.1	Binding	Binding affinity to human histamine H4 receptorBinding affinity to human histamine H4 receptor	ChEMBL	337	5	1	3	5.0	CC(C)(C)c1ccc(SCC(c2ccccn2)c2c[nH]cn2)cc1	10.1021/jm100643t
	5159263	10270	11	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	259	4	3	4	0.8	Cc1cc(C)nc(N/C(N)=N/CCc2c[nH]cn2)n1	10.1021/jm7014777
	CHEMBL1162351	10270	11	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	259	4	3	4	0.8	Cc1cc(C)nc(N/C(N)=N/CCc2c[nH]cn2)n1	10.1021/jm7014777
	71525679	89282	0	None	-6	6	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89282	0	None	-6	6	Mouse	7.1	pKi	=	7.1	Binding	Binding affinity to mouse histamine H4 receptorBinding affinity to mouse histamine H4 receptor	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25178130	189143	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	CHEMBL515870	189143	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm800876b
	25178130	189143	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	CHEMBL515870	189143	2	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	319	3	1	5	3.1	CN1CCN(c2nc(Nc3ccccc3)c3ccccc3n2)CC1	10.1021/jm901379s
	164622153	184976	0	None	-181	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	6	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Cl)cc1	10.1016/j.ejmech.2021.113190
	CHEMBL4863347	184976	0	None	-181	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	394	6	4	4	3.0	Cc1nc(N)sc1CCCN/C(N)=N/C(=O)NC(C)c1ccc(Cl)cc1	10.1016/j.ejmech.2021.113190
	54581939	61200	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770984	61200	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	264	3	1	6	0.7	CC(C)N(C)c1nc(N)cc(N2CCN(C)CC2)n1	10.1016/j.bmcl.2011.03.017
	122193095	123383	0	None	-3	2	Mouse	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assayDisplacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628042	123383	0	None	-3	2	Mouse	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assayDisplacement of [3H]histamine from mouse H4 receptor expressed in HEK293 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 1 hr by liquid scintillation counting assay	ChEMBL	330	3	1	6	2.0	CN1CCN(c2nc(N)nc(/C=C/c3cccc(Cl)c3)n2)CC1	10.1016/j.ejmech.2015.08.014
	58227260	116617	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393546	116617	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	287	3	1	5	1.8	CN1CCN(c2ccnc(NC3CC4CCC3C4)n2)CC1	10.1016/j.bmcl.2014.12.027
	57398176	67971	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	273	2	0	5	1.4	CN1CCN(c2cc(N3[C@@H]4CCC[C@@H]3C4)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916503	67971	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	273	2	0	5	1.4	CN1CCN(c2cc(N3[C@@H]4CCC[C@@H]3C4)ncn2)CC1	10.1016/j.bmcl.2011.07.114
	127050776	139704	0	None	6	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805437	139704	0	None	6	2	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@@H]1CC[C@@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	164627189	185729	0	None	-831	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	298	6	4	4	1.0	CCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	CHEMBL4874758	185729	0	None	-831	4	Human	4.1	pKi	=	4.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	298	6	4	4	1.0	CCCNC(=O)/N=C(\N)NCCCc1sc(N)nc1C	10.1016/j.ejmech.2021.113190
	71519015	86027	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	246	1	2	5	0.7	CN1CCCN(c2nc(N)nc3[nH]ccc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314500	86027	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	246	1	2	5	0.7	CN1CCCN(c2nc(N)nc3[nH]ccc23)CC1	10.1016/j.bmcl.2012.10.139
	57400737	67821	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Cl)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915042	67821	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	331	1	2	6	2.6	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2sc3cc(Cl)ccc3c2n1	10.1016/j.bmcl.2011.08.014
	57395494	67815	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915032	67815	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	349	1	2	6	2.5	Nc1nc(N2C[C@@H]3C[C@H]2CN3)c2oc3cccc(C(F)(F)F)c3c2n1	10.1016/j.bmcl.2011.08.014
	57395499	67824	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	2	7	1.8	C[C@H]1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CCN1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915046	67824	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	1	2	7	1.8	C[C@H]1CN(c2nc(N)nc3c2oc2ncc(Cl)cc23)CCN1	10.1016/j.bmcl.2011.08.014
	46890677	7195	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1086038	7195	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	394	6	1	4	3.8	Cc1cc(OCCCN2CC3C(C2)N3C)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	56851612	71242	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923032	71242	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963245	71242	0	None	-19	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	398	9	2	3	5.3	CCCCN/C(=N/Cc1ccc(Cl)c(Cl)c1)SCCCc1c[nH]cn1	10.1021/jm201042n
	56851602	71244	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1923027	71244	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	CHEMBL1963260	71244	0	None	-18	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation countingDisplacement of [3H]histamine from human H4 receptor expressed in HEK293 cells after 1 to 1.5 hrs by scintillation counting	ChEMBL	364	9	2	3	4.7	CCCCN/C(=N/Cc1ccc(Cl)cc1)SCCCc1c[nH]cn1	10.1021/jm201042n
	25178345	172864	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccco3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	CHEMBL452925	172864	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccco3)c3cc(Cl)ccc3n2)CC1	10.1021/jm800876b
	51354303	58572	0	None	-1	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.7	c1nc(CCCn2cc(C3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	CHEMBL1688971	58572	0	None	-1	2	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	259	5	1	4	2.7	c1nc(CCCn2cc(C3CCCCC3)nn2)c[nH]1	10.1021/jm1013488
	25147771	173656	0	None	-1	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL454890	173656	0	None	-1	3	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	357	4	1	6	3.2	CN1CCN(c2nc(NCc3ccoc3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	10656971	16229	4	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1021/jm7014217
	CHEMBL123137	16229	4	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	228	1	0	4	1.4	CN1CCN(c2cnc3ccccc3n2)CC1	10.1021/jm7014217
	25179123	188501	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	CHEMBL510181	188501	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm901379s
	10197870	5214	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	CHEMBL106612	5214	0	None	-3	2	Human	6.1	pKi	=	6.1	Binding	Histamine H4 receptor competition binding using [3H]Na-methylhistamineHistamine H4 receptor competition binding using [3H]Na-methylhistamine	ChEMBL	167	2	2	3	0.6	NC[C@H]1CC[C@H](c2c[nH]cn2)O1	10.1021/jm0300025
	168273471	192189	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5173079	192189	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	CHEMBL5221433	192189	0	None	-50	4	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysisDisplacement of [3H]-histamine from human histamine H4 receptor stably expressed in baculovirus infected Sf9 cell membrane co-expressing G-alphai2 and G-beta1gamma2 incubated for 60 mins by scintillation counting analysis	ChEMBL	596	17	8	10	0.7	N/C(=N\C(=O)NCCCCCCCCNC(=O)/N=C(\N)NCCCc1nnc(N)s1)NCCCc1nnc(N)s1	10.1021/acs.jmedchem.1c00692
	57402472	67522	0	None	-1819	3	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	526	11	1	6	4.0	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	CHEMBL1910381	67522	0	None	-1819	3	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	ChEMBL	526	11	1	6	4.0	COc1ccc(CN(CCN(C)CCN2CCN(C(=O)c3cc4ccccc4[nH]3)CC2)c2ccccn2)cc1	10.1016/j.bmcl.2011.09.001
	44576985	10269	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	212	4	7	4	-1.3	CC(/C=C(\C)NNC(=N)N)=N/NC(=N)N	10.1021/jm7014777
	CHEMBL1162347	10269	0	None	-	1	Human	6.1	pKi	=	6.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	212	4	7	4	-1.3	CC(/C=C(\C)NNC(=N)N)=N/NC(=N)N	10.1021/jm7014777
	71519010	86022	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	386	2	2	5	2.7	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Br)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314495	86022	0	None	-	1	Human	5.1	pKi	=	5.1	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	386	2	2	5	2.7	CN1CCN(c2nc(N)nc3[nH]c(-c4ccc(Br)cc4)cc23)CC1	10.1016/j.bmcl.2012.10.139
	1204	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	1247	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	1375	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	774	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	111	2	2	2	-0.1	NCCc1cnc[nH]1	10.1016/j.bmcl.2011.10.104
	54587790	61196	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	CHEMBL1770980	61196	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human histamine H4 receptorDisplacement of [3H]histamine from human histamine H4 receptor	ChEMBL	276	4	3	6	1.2	CN[C@@H]1CCN(c2cc(NC3CCCC3)nc(N)n2)C1	10.1016/j.bmcl.2011.03.017
	54753437	67826	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL1915048	67826	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2014.12.027
	11750684	83033	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	CHEMBL219982	83033	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	426	6	1	4	4.9	Cc1ccc2[nH]c(-c3ccc(OCCCN4CCCN(C)CC4)cc3Cl)nc2c1C	10.1016/j.bmcl.2006.08.117
	54753437	67826	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1021/jm401727m
	CHEMBL1915048	67826	0	None	-	1	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1021/jm401727m
	24825794	67699	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	CHEMBL1914549	67699	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	317	1	2	6	2.5	Nc1nc(N2CCC[C@@H](N)C2)c2oc3ccc(Cl)cc3c2n1	10.1016/j.bmcl.2011.08.014
	71525679	89282	0	None	6	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376801	89282	0	None	6	6	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	3	2	6	1.0	CCc1cnc2c(N3CC[C@@H](NC)C3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	25071940	186761	0	None	5	2	Rat	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	CHEMBL493281	186761	0	None	5	2	Rat	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cellsDisplacement of [3H]histamine from rat histamine H4 receptor expressed in HEK293 cells	ChEMBL	281	1	2	5	1.2	Nc1nc2c(c(N3CCNCC3)n1)CCc1ccccc1-2	10.1021/jm800670r
	71525586	89280	0	None	22	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	2	1	6	0.9	CCc1cnc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	CHEMBL2376799	89280	0	None	22	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	272	2	1	6	0.9	CCc1cnc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2013.02.091
	44129135	197739	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	454	6	1	8	1.1	CN1CCN(c2nc(NCCS(=O)(=O)N3CCOCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	CHEMBL591250	197739	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	454	6	1	8	1.1	CN1CCN(c2nc(NCCS(=O)(=O)N3CCOCC3)c3cc(Cl)ccc3n2)CC1	10.1021/jm901379s
	53378711	109648	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	CHEMBL3236578	109648	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysisDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells after 45 mins by competition binding analysis	ChEMBL	235	1	2	5	0.9	CC(C)(C)c1cc(N2CC[C@@H](N)C2)nc(N)n1	10.1021/jm401727m
	54753437	67826	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	CHEMBL1915048	67826	0	None	-	1	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptorDisplacement of [3H]histamine from recombinant human histamine H4 receptor	ChEMBL	318	2	2	7	1.8	CN[C@@H]1CCN(c2nc(N)nc3c2oc2ncc(Cl)cc23)C1	10.1016/j.bmcl.2011.08.014
	135525668	67855	0	None	263	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1915348	67855	0	None	263	2	Human	8.0	pKi	=	8.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	305	1	2	3	1.7	CN1C[C@H]2CN(C(=N)c3nc4cc(F)c(F)cc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	49841611	69375	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935567	69375	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	347	2	1	5	3.2	Cc1cccc(C)c1-c1ccc2c(N3CCN(C)CC3)nc(N)nc2c1	10.1016/j.bmcl.2011.10.104
	1248	1391	27	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	3077	1391	27	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	90045	1391	27	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	CHEMBL12344	1391	27	None	-15	7	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in SK-NM-C cells	ChEMBL	161	4	2	3	0.6	CN(CCCSC(=N)N)C	10.1021/jm7014777
	11615753	72578	6	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3sccc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL200546	72578	6	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3sccc3[nH]2)CC1	10.1021/jm0502081
	10444761	123307	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3ccsc3[nH]2)CC1	10.1021/jm0502081
	CHEMBL362495	123307	0	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cellsDisplacement of [3H]histamine from recombinant human histamine H4 receptor in SK-N-MC cells	ChEMBL	249	1	1	3	1.6	CN1CCN(C(=O)c2cc3ccsc3[nH]2)CC1	10.1021/jm0502081
	16082826	79888	0	None	3	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@H]2C[C@@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	CHEMBL214156	79888	0	None	3	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine form human H4 receptorDisplacement of [3H]histamine form human H4 receptor	ChEMBL	233	5	2	2	2.7	c1nc([C@H]2C[C@@H]2CNCC2CCCCC2)c[nH]1	10.1021/jm0603318
	122193097	123385	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	CHEMBL3628044	123385	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assayDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membranes co-expressing Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting assay	ChEMBL	364	3	1	6	2.7	CN1CCN(c2nc(N)nc(/C=C/c3ccc(Cl)cc3Cl)n2)CC1	10.1016/j.ejmech.2015.08.014
	57390344	67864	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	248	5	3	6	0.3	CNC1CN(c2cc(N)nc(NCC3CC3)n2)C1	10.1016/j.bmcl.2011.07.125
	CHEMBL1915539	67864	0	None	-	1	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]-histamine from full length human H4R expressed in HEK293 cellsDisplacement of [3H]-histamine from full length human H4R expressed in HEK293 cells	ChEMBL	248	5	3	6	0.3	CNC1CN(c2cc(N)nc(NCC3CC3)n2)C1	10.1016/j.bmcl.2011.07.125
	49841607	69363	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	CHEMBL1935555	69363	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	257	1	1	5	1.3	Cc1cccc2c(N3CCN(C)CC3)nc(N)nc12	10.1016/j.bmcl.2011.10.104
	12686451	202342	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL1743969	202342	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	CHEMBL6992	202342	2	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	262	1	0	4	2.0	CN1CCN(c2cnc3cc(Cl)ccc3n2)CC1	10.1021/jm7014217
	51354010	58561	0	None	-1	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	281	7	1	4	2.6	c1ccc(Cc2cn(CCCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	CHEMBL1688941	58561	0	None	-1	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293 cells after 60 mins by liquid scintillation counting	ChEMBL	281	7	1	4	2.6	c1ccc(Cc2cn(CCCCc3c[nH]cn3)nn2)cc1	10.1021/jm1013488
	127036979	136950	0	None	1	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3753962	136950	0	None	1	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	399	5	1	5	3.6	CN1CCN(c2nc(NCCc3ccccc3)nc3c2CCc2ccccc2-3)CC1	10.1016/j.bmcl.2015.12.035
	118720434	115398	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	241	3	2	5	0.6	CN[C@@H]1CCN(c2cc(CCl)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	CHEMBL3354808	115398	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	241	3	2	5	0.6	CN[C@@H]1CCN(c2cc(CCl)nc(N)n2)C1	10.1016/j.bmcl.2014.10.013
	11212855	83801	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	CHEMBL220934	83801	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cellsDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in SK-N-MC cells	ChEMBL	440	6	1	4	5.2	CN1CCN(CCCOc2ccc(-c3nc4cc(C(C)(C)C)ccc4[nH]3)c(Cl)c2)CC1	10.1016/j.bmcl.2006.08.117
	57401602	67972	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	242	1	0	4	1.3	Cc1ccc(C#N)c(N2CC3CN(C)CC3C2)n1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916504	67972	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	242	1	0	4	1.3	Cc1ccc(C#N)c(N2CC3CN(C)CC3C2)n1	10.1016/j.bmcl.2011.07.114
	156010537	177724	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4634235	177724	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	CHEMBL4651053	177724	0	None	-69	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranesDisplacement of [3H]-histamine from Galphai2/Gbeta1gamma2-coupled human recombinant H4R expressed in baculovirus infected Sf9 insect cell membranes	ChEMBL	430	11	2	3	4.3	Cc1cc(/C=C/C=C/c2ccc(N(C)C)cc2)cc(C)[n+]1CCCNCCc1c[nH]cn1	10.1021/acs.jmedchem.0c00160
	90654503	109810	0	None	-123	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	483	9	1	4	4.9	O=C1NC(c2ccccc2)(c2ccccc2)C(=O)N1Cc1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	CHEMBL3238447	109810	0	None	-123	3	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysisDisplacement of [3H]histamine from human H4 receptor expressed in Sf9 cell membrane co-transfected with Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting analysis	ChEMBL	483	9	1	4	4.9	O=C1NC(c2ccccc2)(c2ccccc2)C(=O)N1Cc1ccc(OCCCN2CCCCC2)cc1	10.1016/j.ejmech.2014.03.014
	57394626	67961	0	None	14	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	CHEMBL1916494	67961	0	None	14	2	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting techniqueDisplacement of [3H]histamine from human histamine H4 receptor expressed in CHO cells after 90 mins by scintillation counting technique	ChEMBL	263	5	3	5	2.1	CC(C)(C)CCNc1cc(N[C@@H]2CCNC2)ncn1	10.1016/j.bmcl.2011.07.114
	49841723	69368	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)cc1	10.1016/j.bmcl.2011.10.104
	CHEMBL1935560	69368	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human H4 receptor expressed in HEK cell membranesDisplacement of [3H]histamine from human H4 receptor expressed in HEK cell membranes	ChEMBL	349	3	1	6	2.6	COc1ccc(-c2cccc3c(N4CCN(C)CC4)nc(N)nc23)cc1	10.1016/j.bmcl.2011.10.104
	112936251	136729	1	None	1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752085	136729	1	None	1	4	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	359	5	1	5	3.5	CN1CCN(c2cc(-c3ccccc3)nc(NCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	25178340	186193	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm800876b
	CHEMBL489069	186193	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm800876b
	25179123	188501	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	CHEMBL510181	188501	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK cells	ChEMBL	363	5	1	7	2.0	CCOC(=O)CNc1nc(N2CCN(C)CC2)nc2ccc(Cl)cc12	10.1021/jm800876b
	25178340	186193	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm901379s
	CHEMBL489069	186193	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	291	2	1	5	2.1	CNc1nc(N2CCN(C)CC2)nc2cc(Cl)ccc12	10.1021/jm901379s
	164618690	185564	0	None	-13	5	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	3.4	CCCCCCNC(=O)/N=C(\N)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	CHEMBL4872375	185564	0	None	-13	5	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting methodDisplacement of [3H]histamine from human histamine H4 receptor expressed in sf9 insect cell membranes co-expressing Galphai2/Gbeta1gamma2 incubated for 60 mins by microbeta scintillation counting method	ChEMBL	360	7	4	4	3.4	CCCCCCNC(=O)/N=C(\N)Nc1cccc(-c2csc(N)n2)c1	10.1016/j.ejmech.2021.113190
	44547126	116598	1	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393527	116598	1	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	248	5	2	4	1.9	CN[C@H]1CCN(c2ccnc(NCC(C)C)c2)C1	10.1016/j.bmcl.2014.12.027
	118725540	116595	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	1.9	CN[C@@H]1CCN(c2ccnc(N3CCCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	CHEMBL3393524	116595	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	260	3	1	4	1.9	CN[C@@H]1CCN(c2ccnc(N3CCCCC3)c2)C1	10.1016/j.bmcl.2014.12.027
	46890676	7148	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	1	4	4.0	Cc1cc(OCCCN2CC3CN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	CHEMBL1085804	7148	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptorDisplacement of [3H]histamine from human recombinant histamine H4 receptor	ChEMBL	408	6	1	4	4.0	Cc1cc(OCCCN2CC3CN(C)C3C2)ccc1-c1nc2c(C)c(F)ccc2[nH]1	10.1016/j.bmcl.2010.04.017
	57393651	69549	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3cc4cccnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	CHEMBL1938971	69549	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assayDisplacement of [3H]histamine from human recombinant histamine H4 receptor expressed in CHO cells coexpressing Ga15 by radioligand filtration binding assay	ChEMBL	270	1	1	3	1.2	CN1C[C@H]2CN(C(=O)c3cc4cccnc4[nH]3)C[C@H]2C1	10.1016/j.bmcl.2011.11.098
	24853666	155565	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	1	1	4	1.7	CN1CCCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	CHEMBL406327	155565	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cellsDisplacement of [3H]histamine from human histamine H4 receptor expressed in HEK293T cells	ChEMBL	292	1	1	4	1.7	CN1CCCN(c2nc3cc(Cl)ccc3[nH]c2=O)CC1	10.1021/jm7014217
	112936267	136758	1	None	-2	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2cc(-c3ccccc3)nc(NCCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	CHEMBL3752383	136758	1	None	-2	2	Human	5.0	pKi	=	5.0	Binding	Displacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation countingDisplacement of [3H]histamine from human histamine 4 receptor expressed in Sf9 cell membranes by liquid scintillation counting	ChEMBL	373	6	1	5	3.6	CN1CCN(c2cc(-c3ccccc3)nc(NCCc3ccccc3)n2)CC1	10.1016/j.bmcl.2015.12.035
	127051301	139657	0	None	5	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3804906	139657	0	None	5	2	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	246	3	3	3	0.9	CN/C(=N\C#N)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	25130914	186661	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	CHEMBL492677	186661	0	None	-	1	Human	7.0	pKi	=	7.0	Binding	Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2Displacement of [3H]histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai2 and Gbeta1gamma2	ChEMBL	298	4	2	6	1.4	CN1CCN(c2cc(NCc3ccccc3)nc(N)n2)CC1	10.1016/j.bmc.2009.08.059
	71519178	86044	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	CHEMBL2314761	86044	0	None	-	1	Human	6.0	pKi	=	6.0	Binding	Displacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gammaDisplacement of [3H]Histamine from human histamine H4 receptor expressed in Sf9 cells co-expressing Galphai/o and Gbeta1gamma	ChEMBL	312	2	3	5	1.9	Nc1nc(N2CC[C@H](N)C2)c2cc(-c3ccc(F)cc3)[nH]c2n1	10.1016/j.bmcl.2012.10.139
	127052293	139695	1	None	34	4	Human	7.0	pKi	=	7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	CHEMBL3805361	139695	1	None	34	4	Human	7.0	pKi	=	7	Binding	Displacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting methodDisplacement of [3H]UR-PI294 from human histamine H4 receptor expressed in sf9 cell membrane co-expressing mammalian Galphai2 and Gbeta1gamma2 incubated for 60 mins by liquid scintillation counting method	ChEMBL	368	7	3	4	3.1	N#C/N=C(/NCCSc1ccccc1)NC[C@H]1CC[C@H](c2c[nH]cn2)C1	10.1021/acs.jmedchem.6b00120
	1530	2142	44	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	3827	2142	44	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	7206	2142	44	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	CHEMBL534	2142	44	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	DB00920	2142	44	None	-21379	21	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	309	0	0	3	4.0	CN1CCC(=C2c3ccccc3CC(=O)c3c2ccs3)CC1	None
	124087	1357	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	7157	1357	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	814	1357	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	CHEMBL1172	1357	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	DB00967	1357	106	None	-5888	15	Human	8.3	pIC50	=	8.3	Binding	Histamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding AssayHistamine H-4 Receptor Binding Assay: Histamine H-4 Receptor Binding Assay	Drug Central	310	0	1	2	4.0	Clc1ccc2c(c1)CCc1c(C2=C2CCNCC2)nccc1	None
	71548412	1870	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	9879	1870	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	CHEMBL4790724	1870	14	None	-	0	Mouse	6.5	pIC50	=	6.5	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing mouse H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	71548412	1870	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	9879	1870	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	CHEMBL4790724	1870	14	None	-	0	Human	7.6	pIC50	=	7.6	Binding	Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.Radioligand competitive binding assay using [<sup>3</sup>H]Histamine as tracer, and membrane preparations from CHO-K1 cells expressing human H<sub>4</sub> receptor.	Guide to Pharmacology	334	2	1	8	0.2	CNC1CN(C1)c1nc2ncc(cc2n2c1nnn2)Br	29579390
	1278	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1279	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	4908365	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	CHEMBL129198	2110	79	None	-3	8	Human	8.4	pKd	=	8.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1204	1893	114	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1893	114	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1893	114	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1893	114	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1893	114	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1893	114	None	-12	25	Rat	6.9	pKd	None	6.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1236	2028	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1237	2028	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	156615	2028	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	CHEMBL268229	2028	23	None	-204	7	Human	7.2	pKd	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1204	1893	114	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1893	114	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1893	114	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1893	114	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1893	114	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1893	114	None	-15	25	Mouse	7.4	pKd	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1241	1998	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1242	1998	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	3614	1998	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	CHEMBL507	1998	46	None	-89	5	Human	7.8	pKd	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1204	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1204	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1204	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1204	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1204	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1247	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1247	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1247	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1247	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1247	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1375	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	1375	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1375	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	1375	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1375	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	774	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	774	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	774	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	774	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	774	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	DB05381	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	10973974
	DB05381	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	DB05381	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179435
	DB05381	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	DB05381	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1893	114	None	-3	25	Human	7.9	pKd	None	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	None	214362	0	3H-Histamine	4	7	Guinea pig	10.0	pKi	=	10	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	1204	1893	114	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-Histamine	1	25	Guinea pig	9.9	pKi	=	9.9	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	None	214362	0	3H-Histamine	4	7	Guinea pig	9.8	pKi	=	9.8	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	179	396	107	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	2159	396	107	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	963	396	107	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	CHEMBL243712	396	107	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	DB06288	396	107	3H-Histamine	-4365	51	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	369	7	2	6	1.3	CCN1CCCC1CNC(=O)c1cc(c(cc1OC)N)S(=O)(=O)CC	None
	242	467	117	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	34	467	117	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	60795	467	117	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	CHEMBL1112	467	117	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	DB01238	467	117	3H-Histamine	-7244	52	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	447	7	1	4	4.9	O=C1CCc2c(N1)cc(cc2)OCCCCN1CCN(CC1)c1cccc(c1Cl)Cl	None
	1231	922	112	3H-Histamine	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	1231	922	112	3H-PYRILAMINE	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	2756	922	112	3H-Histamine	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	2756	922	112	3H-PYRILAMINE	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	645	922	112	3H-Histamine	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	645	922	112	3H-PYRILAMINE	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	CHEMBL30	922	112	3H-Histamine	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	CHEMBL30	922	112	3H-PYRILAMINE	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	DB00501	922	112	3H-Histamine	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	DB00501	922	112	3H-PYRILAMINE	-81	8	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	252	5	3	4	0.6	N#CNC(=NC)NCCSCc1nc[nH]c1C	None
	277	1269	55	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1269	55	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1269	55	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1269	55	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1269	55	3H-Histamine	-301	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1248	1391	27	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	3077	1391	27	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	90045	1391	27	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	CHEMBL12344	1391	27	3H-PYRILAMINE	-15	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	1224	1399	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	3100	1399	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	8980	1399	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	916	1399	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	CHEMBL657	1399	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	DB01075	1399	79	3H-Histamine	-194	13	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	1212	1624	45	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	204	1624	45	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	3372	1624	45	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	CHEMBL726	1624	45	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	DB00623	1624	45	3H-Histamine	-19952	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	437	6	1	5	4.3	OCCN1CCN(CC1)CCCN1c2ccccc2Sc2c1cc(cc2)C(F)(F)F	None
	1353	1872	85	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	3559	1872	85	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	86	1872	85	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	CHEMBL54	1872	85	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	DB00502	1872	85	3H-Histamine	-6918	86	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	375	6	1	3	4.4	Fc1ccc(cc1)C(=O)CCCN1CCC(CC1)(O)c1ccc(cc1)Cl	None
	135398745	2858	108	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	47	2858	108	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	CHEMBL715	2858	108	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	DB00334	2858	108	3H-Histamine	-5128	66	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	312	1	1	5	1.7	CN1CCN(CC1)C1=c2cc(sc2=Nc2c(N1)cccc2)C	None
	119570	3098	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	2233	3098	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	953	3098	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	CHEMBL301265	3098	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	DB00413	3098	90	3H-Histamine	-301	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	211	3	2	4	1.6	CCCN[C@H]1CCc2c(C1)sc(n2)N	None
	2337	3193	72	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	50	3193	72	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	5002	3193	72	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	CHEMBL716	3193	72	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	DB01224	3193	72	3H-Histamine	-1122	63	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	383	5	1	6	2.9	OCCOCCN1CCN(CC1)C1=Nc2ccccc2Sc2c1cccc2	None
	1234	3227	0	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	3001055	3227	0	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	CHEMBL512	3227	0	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	DB00863	3227	0	3H-Histamine	-190	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	314	10	2	7	1.5	CN/C(=C\[N+](=O)[O-])/NCCSCc1ccc(o1)CN(C)C	None
	2389	3267	114	3H-Histamine	-32359	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	5073	3267	114	3H-Histamine	-32359	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	96	3267	114	3H-Histamine	-32359	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	CHEMBL85	3267	114	3H-Histamine	-32359	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	DB00734	3267	114	3H-Histamine	-32359	68	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	410	4	0	6	3.6	Fc1ccc2c(c1)onc2C1CCN(CC1)CCc1c(C)nc2n(c1=O)CCCC2	None
	6917970	3622	54	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	8370	3622	54	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	CHEMBL487387	3622	54	3H-Histamine	-2290	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	327	2	2	5	2.8	COc1cc2CCN3[C@H](c2cc1O)Cc1c(C3)c(OC)c(cc1)O	None
	213	3780	50	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	2717	3780	50	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	5533	3780	50	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	CHEMBL621	3780	50	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	DB00656	3780	50	3H-Histamine	-912	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	371	5	0	6	2.4	Clc1cccc(c1)N1CCN(CC1)CCCn1nc2n(c1=O)cccc2	None
	185	3931	54	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	5311271	3931	54	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	CHEMBL74355	3931	54	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	DB16351	3931	54	3H-Histamine	-30902	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	1	4	3.8	COc1cccc(c1OC)[C@@H](C1CCN(CC1)CCc1ccc(cc1)F)O	None
	2865	4064	67	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	59	4064	67	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	60854	4064	67	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	CHEMBL708	4064	67	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	DB00246	4064	67	3H-Histamine	-20417	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	412	4	1	5	3.8	O=C1Nc2c(C1)cc(c(c2)Cl)CCN1CCN(CC1)c1nsc2c1cccc2	None
	10297	26890	29	3H-Histamine	-38	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	C[C@H](N)[C@H](O)c1ccccc1	None
	CHEMBL136560	26890	29	3H-Histamine	-38	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	C[C@H](N)[C@H](O)c1ccccc1	None
	156391	46522	95	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	CHEMBL1200806	46522	95	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	CHEMBL154	46522	95	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	COc1ccc2cc([C@H](C)C(=O)O)ccc2c1	None
	10624	69942	17	3H-Histamine	-776	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	138543650	69942	17	3H-Histamine	-776	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	CHEMBL194378	69942	17	3H-Histamine	-776	33	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	284	5	3	3	1.7	CN(C)CCc1c[nH]c2cccc(OP(=O)(O)O)c12	None
	21830793	91389	5	3H-8-OH-DPAT	-66069	46	Bovine	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	0	8	0.6	COc1ccccc1N1CCN(CCCCn2ncc(=O)n(C)c2=O)CC1	None
	CHEMBL2413154	91389	5	3H-8-OH-DPAT	-66069	46	Bovine	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	373	7	0	8	0.6	COc1ccccc1N1CCN(CCCCn2ncc(=O)n(C)c2=O)CC1	None
	46780481	107032	18	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	9903970	107032	18	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	CHEMBL3187365	107032	18	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	CHEMBL3544974	107032	18	3H-Histamine	-58884	54	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	285	0	0	2	4.3	CN1CC2c3ccccc3Oc3ccc(Cl)cc3C2C1	None
	446220	132985	13	3H-Histamine	-1778	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	303	3	0	5	1.9	COC(=O)[C@H]1[C@@H](OC(=O)c2ccccc2)C[C@@H]2CC[C@H]1N2C	None
	CHEMBL370805	132985	13	3H-Histamine	-1778	46	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	303	3	0	5	1.9	COC(=O)[C@H]1[C@@H](OC(=O)c2ccccc2)C[C@@H]2CC[C@H]1N2C	None
	4495	194850	87	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	308	5	1	5	2.8	CS(=O)(=O)Nc1ccc([N+](=O)[O-])cc1Oc1ccccc1	None
	CHEMBL56367	194850	87	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	308	5	1	5	2.8	CS(=O)(=O)Nc1ccc([N+](=O)[O-])cc1Oc1ccccc1	None
	54677470	198857	110	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	CHEMBL1256873	198857	110	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	CHEMBL599	198857	110	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	351	2	2	6	2.0	Cc1cnc(NC(=O)C2=C(O)c3ccccc3S(=O)(=O)N2C)s1	None
	162265	200578	19	3H-Histamine	-239	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	4786	200578	19	3H-Histamine	-239	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	CHEMBL61006	200578	19	3H-Histamine	-239	45	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	151	2	2	2	1.1	CC(N)C(O)c1ccccc1	None
	4054	203780	64	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	CHEMBL1699	203780	64	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	CHEMBL807	203780	64	3H-Histamine	-1	37	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	179	0	1	1	2.7	CC12CC3CC(C)(C1)CC(N)(C3)C2	None
	11954259	214191	0	3H-Histamine	-33113	44	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	443	5	0	5	3.5	CN1CCN(CC1)CCC=C2C3=CC=CC=C3SC4=C2C=C(C=C4)S(=O)(=O)N(C)C	None
	None	214207	0	3H-Histamine	-1	28	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	329	2	0	4	3.6	CC1=NC=CN1CC2CCC3=C(C2=O)C4=CC=CC=C4N3C.Cl	None
	104911	214239	0	3H-Histamine	-41686	38	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	530	7	0	5	5.1	COC1=CC=CC=C1N2CCN(CC2)CCN(C3=CC=CC=N3)C(=O)C4CCCCC4.Cl.Cl.Cl	None
	None	214362	0	3H-PYRILAMINE	-7585	7	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	25137849	214415	0	3H-Histamine	-4	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	165	3	2	2	1.3	CC(C(C1=CC=CC=C1)O)NC	None
	71290	214415	0	3H-Histamine	-4	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	165	3	2	2	1.3	CC(C(C1=CC=CC=C1)O)NC	None
	None	214550	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	240	7	4	6	-0.8	C(C(C(=O)O)N)SSCC(C(=O)O)N	None
	None	214551	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	149	4	2	3	0.2	CSCCC(C(=O)O)N	None
	None	214552	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	135	3	3	3	-0.3	C(CS)C(C(=O)O)N	None
	None	214553	0	3H-Histamine	-1	34	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	121	2	3	3	-0.7	C(C(C(=O)O)N)S	None
	None	214554	0	3H-Histamine	-1	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	117	0	1	3	-0.0	C1CSC(=O)C1N	None
	None	214555	0	3H-Histamine	-1	40	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	153	3	3	3	-1.4	C(C(C(=O)O)N)S(=O)O	None
	None	214556	0	3H-Histamine	-1	39	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	169	3	3	4	-1.7	C(C(C(=O)O)N)S(=O)(=O)O	None
	None	214564	0	3H-Histamine	-13	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	149	2	1	2	1.2	CC(C(=O)C1=CC=CC=C1)N	None
	1576	214565	0	3H-Histamine	-16	41	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	163	3	1	2	1.5	CC(C(=O)C1=CC=CC=C1)NC	None
	135269	214629	0	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	222	5	1	3	2.5	CCCCC(=O)OC1=CC=CC=C1C(=O)O	None
	23681059	214630	0	3H-Histamine	-1	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	230	3	1	2	3.0	CC(C1=CC2=C(C=C1)C=C(C=C2)OC)C(=O)O	None
	123619	214634	0	3H-Histamine	-1412	27	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	358	3	0	4	4.2	CC1=NC=C(C=C1)C2=NC=C(C=C2C3=CC=C(C=C3)S(=O)(=O)C)Cl	None
	119828	214635	0	3H-Histamine	-1	30	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	370	5	1	5	3.5	CCC(=O)NS(=O)(=O)C1=CC=C(C=C1)C2=C(ON=C2C3=CC=CC=C3)C	None
	None	214638	0	3H-Histamine	-7	29	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	517	8	2	5	5.2	CC1=C(C2=C(N1C(=O)C3=CC=C(C=C3)Cl)C=CC(=C2)OC)CC(=O)NCCC4=CC=C(C=C4)NC(=O)C	None
	None	214737	0	3H-Histamine	-4570	27	Human	5.0	pKi	=	5	Binding	NoneNone	PDSP KiDatabase	347	6	0	3	5.0	CC(=O)N(CC1=CC=CC=C1OC)C2=CC=CC=C2OC3=CC=CC=C3	None
	135409468	1996	63	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	333	1996	63	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	CHEMBL845	1996	63	3H-Histamine	-363	40	Human	6.0	pKi	=	6.0	Binding	NoneNone	PDSP KiDatabase	312	1	2	4	1.7	Clc1ccc2c(c1)NC(=c1c(=N2)cccc1)N1CCNCC1	None
	1204	1893	114	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-Histamine	1	25	Guinea pig	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1225	1438	24	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	3958	1438	24	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	667477	1438	24	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	CHEMBL860	1438	24	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	DB01142	1438	24	3H-PYRILAMINE	-371	22	Human	7.0	pKi	=	7.0	Binding	NoneNone	PDSP KiDatabase	279	3	0	2	4.0	CN(CC/C=C\1/c2ccccc2OCc2c1cccc2)C	None
	1226	1980	16	3H-Histamine	2	7	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	41376	1980	16	3H-Histamine	2	7	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	CHEMBL12608	1980	16	3H-Histamine	2	7	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	1223	938	32	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	938	32	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	938	32	3H-JNJ 7777120	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1251	1975	34	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1975	34	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1975	34	3H-JNJ 7777120	-100	5	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1253	2019	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	132937	2019	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	CHEMBL19010	2019	7	3H-N-alpha-methylhistamine	-11	4	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	342	6	1	2	3.2	Ic1ccc(cc1)COCCCc1cnc[nH]1	None
	None	214492	0	3H-Histamine	-13	21	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	None	214492	0	3H-N-alpha-methylhistamine	-13	21	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	1223	938	32	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	938	32	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	938	32	3H-Histamine	-60	11	Human	7.9	pKi	=	7.9	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1267	3732	43	3H-Histamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3732	43	3H-N-alpha-methylhistamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	3H-Histamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	3H-N-alpha-methylhistamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	3H-Histamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	3H-N-alpha-methylhistamine	-33	28	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1547484	925	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	654	925	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	9072	925	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	CHEMBL43064	925	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	DB00568	925	70	3H-PYRILAMINE	-5	20	Human	6.9	pKi	=	6.9	Binding	NoneNone	PDSP KiDatabase	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	45037031	214385	0	3H-Histamine	-70	4	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@H](CC1=CN=CN1)N.Br.Br	None
	1223	938	32	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	938	32	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	938	32	125I-iodophenpropit	-60	11	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1204	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1893	114	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-N-alpha-methylhistamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	2286	3122	48	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	4927	3122	48	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	7282	3122	48	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	CHEMBL643	3122	48	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	DB01069	3122	48	3H-PYRILAMINE	-10	30	Human	6.8	pKi	=	6.8	Binding	NoneNone	PDSP KiDatabase	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	1204	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-Histamine	-3	25	Human	7.8	pKi	=	7.8	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	3032915	15306	20	3H-Histamine	-1	7	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15306	20	3H-Histamine	-1	7	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	1204	1893	114	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-JNJ 7777120	-3	25	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1251	1975	34	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1251	1975	34	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	1251	1975	34	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1975	34	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1975	34	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1975	34	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1975	34	125I-iodophenpropit	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1975	34	3H-Histamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1975	34	3H-N-alpha-methylhistamine	-100	5	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	None	214492	0	125I-iodophenpropit	-13	21	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	None	214492	0	3H-JNJ 7777120	-13	21	Human	7.7	pKi	=	7.7	Binding	NoneNone	PDSP KiDatabase	416	9	3	4	2.8	C1=CC(=CC=C1CCNC(N)SCCCC2=CN=CN2)I	None
	103	4074	56	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2875	4074	56	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	5736	4074	56	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL285802	4074	56	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB09225	4074	56	3H-Histamine	-89	54	Human	5.7	pKi	=	5.7	Binding	NoneNone	PDSP KiDatabase	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	135398737	942	89	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	3H-Histamine	-57	92	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	1204	1893	114	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-Histamine	-3	25	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	11957573	1971	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1971	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1971	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1971	29	3H-Histamine	-40	6	Human	8.6	pKi	=	8.6	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	277	1269	55	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1269	55	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1269	55	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1269	55	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1269	55	3H-PYRILAMINE	-301	46	Human	6.7	pKi	=	6.7	Binding	NoneNone	PDSP KiDatabase	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1241	1998	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	1242	1998	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	3614	1998	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	CHEMBL507	1998	46	3H-Histamine	-89	5	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	125	2	1	3	-0.1	Cn1cc(nc1)CCN	None
	None	214362	0	3H-Histamine	-7585	7	Human	6.6	pKi	=	6.6	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	100	3733	52	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	2637	3733	52	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	5452	3733	52	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	CHEMBL479	3733	52	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	DB00679	3733	52	3H-Histamine	-56	56	Human	5.6	pKi	=	5.6	Binding	NoneNone	PDSP KiDatabase	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	1204	1893	114	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	125I-iodophenpropit	-3	25	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1226	1980	16	3H-N-alpha-methylhistamine	2	7	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	41376	1980	16	3H-N-alpha-methylhistamine	2	7	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	CHEMBL12608	1980	16	3H-N-alpha-methylhistamine	2	7	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	None
	45037031	214385	0	3H-N-alpha-methylhistamine	-70	4	Human	6.6	pKi	=	6.6	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@H](CC1=CN=CN1)N.Br.Br	None
	1267	3732	43	3H-Histamine	-33	28	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	3H-Histamine	-33	28	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	3H-Histamine	-33	28	Human	7.6	pKi	=	7.6	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1210	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	1213	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2725	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	33036	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	4411	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	616	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	6976	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	716121	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	90475904	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1201353	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1554789	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL505	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB01114	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB13679	903	45	3H-PYRILAMINE	-186	21	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	135398737	942	89	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	125I-iodophenpropit	-57	92	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	1239	1994	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	1240	1994	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	912	1994	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	CHEMBL268490	1994	23	3H-N-alpha-methylhistamine	-154	5	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	3	2	2	0.2	CNCCc1cnc[nH]1	None
	180	397	50	3H-PYRILAMINE	-32	39	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	200	397	50	3H-PYRILAMINE	-32	39	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	2160	397	50	3H-PYRILAMINE	-32	39	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	CHEMBL629	397	50	3H-PYRILAMINE	-32	39	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	DB00321	397	50	3H-PYRILAMINE	-32	39	Human	7.5	pKi	=	7.5	Binding	NoneNone	PDSP KiDatabase	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	1204	1893	114	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-PYRILAMINE	-3	25	Human	5.5	pKi	=	5.5	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	None	214362	0	3H-Histamine	-7585	7	Human	6.5	pKi	=	6.5	Binding	NoneNone	PDSP KiDatabase	125	2	2	2	0.3	CC(CC1=CN=CN1)N	None
	1248	1391	27	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	3077	1391	27	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	90045	1391	27	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	CHEMBL12344	1391	27	3H-Histamine	-15	7	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	161	4	2	3	0.6	CN(CCCSC(=N)N)C	None
	11957573	1971	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1971	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1971	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1971	29	3H-PYRILAMINE	-40	6	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	921599	19240	44	3H-Histamine	234	2	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	None
	CHEMBL129660	19240	44	3H-Histamine	234	2	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	229	1	2	2	1.2	O=C(c1cc2ccccc2[nH]1)N1CCNCC1	None
	1204	1893	114	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-Histamine	-3	25	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1267	3732	43	125I-iodophenpropit	-33	28	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	125I-iodophenpropit	-33	28	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	125I-iodophenpropit	-33	28	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3032915	15306	20	3H-Histamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	3032915	15306	20	3H-N-alpha-methylhistamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15306	20	3H-Histamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	CHEMBL12160	15306	20	3H-N-alpha-methylhistamine	-1	7	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	212	5	3	2	1.2	C/N=C(\S)NCCCCc1c[nH]cn1	None
	135398737	942	89	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	3H-JNJ 7777120	-57	92	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	45073439	214386	0	3H-N-alpha-methylhistamine	-75	4	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	285	2	2	2	1.5	C[C@@H](CC1=CN=CN1)N.Br.Br	None
	1224	1399	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	3100	1399	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	8980	1399	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	916	1399	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	CHEMBL657	1399	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	DB01075	1399	79	None	-194	13	Human	8.4	pKi	=	8.4	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	255	6	0	2	3.4	CN(CCOC(c1ccccc1)c1ccccc1)C	None
	2782	31769	62	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL1407943	31769	62	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL3216363	31769	62	3H-PYRILAMINE	-	1	Human	6.4	pKi	=	6.4	Binding	NoneNone	PDSP KiDatabase	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	1223	938	32	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	938	32	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	938	32	3H-Histamine	-60	11	Human	7.4	pKi	=	7.4	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	4761	13315	39	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1193	13315	39	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1580221	13315	39	3H-PYRILAMINE	-	1	Human	5.4	pKi	=	5.4	Binding	NoneNone	PDSP KiDatabase	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	1267	3732	43	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3732	43	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	3H-Histamine	-33	28	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	115237	55281	113	None	-891	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	CHEMBL1621	55281	113	None	-891	55	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	2247	502	77	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	249	502	77	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	2603	502	77	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	CHEMBL296419	502	77	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	DB00637	502	77	None	-117	42	Human	8.3	pKi	=	8.3	Binding	Displacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation countingDisplacement of [3H]histamine from human H4R expressed in Sf9 cells co-expressing RGS19, Galphai2 and Gbeta1gamma2 after 60 mins by liquid scintillation counting	Drug Central	458	8	1	5	5.4	COc1ccc(cc1)CCN1CCC(CC1)Nc1nc2c(n1Cc1ccc(cc1)F)cccc2	None
	5625	215961	0	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	456	6	3	6	2.7	CC(C)NC1=C(N=CC=C1)N1CCN(CC1)C(=O)C1=CC2=C(N1)C=CC(NS(C)(=O)=O)=C2	None
	1613	2306	44	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	205	2306	44	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	3964	2306	44	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	CHEMBL831	2306	44	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	DB00408	2306	44	None	-134	45	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	16106	117630	28	None	-20	12	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	None
	CHEMBL340801	117630	28	None	-20	12	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	291	0	0	3	2.9	CN1CCN(C2=Nc3ccccc3Cc3ccccc32)CC1	None
	4761	13315	39	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1193	13315	39	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	CHEMBL1580221	13315	39	None	-	1	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	240	5	0	2	3.2	CN(C)CCC(c1ccccc1)c1ccccn1	None
	135398737	942	89	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	None	-54	92	Mouse	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	100	3733	52	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	2637	3733	52	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	5452	3733	52	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	CHEMBL479	3733	52	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	DB00679	3733	52	None	-56	56	Human	8.3	pKi	=	8.3	Binding	NoneNone	Drug Central	370	4	0	4	5.9	CSc1ccc2c(c1)N(CCC1CCCCN1C)c1c(S2)cccc1	None
	16362	3064	67	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	2172	3064	67	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	90	3064	67	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	CHEMBL1423	3064	67	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	DB01100	3064	67	None	-562	30	Human	8.2	pKi	=	8.2	Binding	Antagonist activity at H4 receptor (unknown origin)Antagonist activity at H4 receptor (unknown origin)	Drug Central	461	7	1	3	5.9	Fc1ccc(cc1)C(c1ccc(cc1)F)CCCN1CCC(CC1)n1c(=O)[nH]c2c1cccc2	None
	135398737	942	89	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	None	-44	92	Rat	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	103	4074	56	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2875	4074	56	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	5736	4074	56	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL285802	4074	56	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB09225	4074	56	None	-89	54	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	331	4	0	3	4.9	CN(CCOC1=Cc2ccccc2Sc2c1cc(Cl)cc2)C	None
	2726	904	64	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	904	64	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	904	64	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	904	64	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	904	64	3H-PYRILAMINE	-70	73	Human	7.3	pKi	=	7.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	2726	904	64	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	904	64	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	904	64	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	904	64	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	904	64	3H-Histamine	-70	73	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	1613	2306	44	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	205	2306	44	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	3964	2306	44	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	CHEMBL831	2306	44	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	DB00408	2306	44	3H-Histamine	-134	45	Human	5.3	pKi	=	5.3	Binding	NoneNone	PDSP KiDatabase	327	0	0	4	3.8	CN1CCN(CC1)C1=Nc2ccccc2Oc2c1cc(Cl)cc2	None
	135398737	942	89	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	3H-Histamine	-57	92	Human	6.3	pKi	=	6.3	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	11957573	1971	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	1250	1971	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	3692	1971	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	CHEMBL19439	1971	29	3H-N-alpha-methylhistamine	-40	6	Human	8.2	pKi	=	8.2	Binding	NoneNone	PDSP KiDatabase	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	None
	2782	31769	62	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL1407943	31769	62	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	CHEMBL3216363	31769	62	None	-	1	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	325	4	0	3	4.3	Clc1ccc(Cn2c(CN3CCCC3)nc3ccccc32)cc1	None
	1210	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	1213	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2725	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	33036	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	4411	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	616	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	6976	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	716121	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	90475904	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1201353	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL1554789	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	CHEMBL505	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB01114	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	DB13679	903	45	None	-186	21	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	274	5	0	2	3.8	CN(CCC(c1ccccn1)c1ccc(cc1)Cl)C	None
	2286	3122	48	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	4927	3122	48	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	7282	3122	48	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	CHEMBL643	3122	48	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	DB01069	3122	48	None	-10	30	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	284	3	0	3	4.2	CN(C(CN1c2ccccc2Sc2c1cccc2)C)C	None
	2726	904	64	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	621	904	64	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	83	904	64	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	CHEMBL71	904	64	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	DB00477	904	64	None	-70	73	Human	8.2	pKi	=	8.2	Binding	NoneNone	Drug Central	318	4	0	3	4.9	CN(CCCN1c2ccccc2Sc2c1cc(Cl)cc2)C	None
	1204	1893	114	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	None	-15	25	Mouse	8.2	pKi	=	8.2	Binding	Binding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to mouse histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1893	114	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	None	-12	25	Rat	8.1	pKi	=	8.1	Binding	Binding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assayBinding affinity to rat histamine H4 receptor expressed in human SK-N-MC cells by radioligand displacement assay	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1223	938	32	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	1223	938	32	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	938	32	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	2790	938	32	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	938	32	3H-Histamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	CHEMBL14690	938	32	3H-N-alpha-methylhistamine	-60	11	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	None
	135	2486	38	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1796	2486	38	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	4184	2486	38	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	CHEMBL6437	2486	38	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	DB06148	2486	38	3H-PYRILAMINE	-389	58	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	264	0	0	2	3.1	CN1CCN2C(C1)c1ccccc1Cc1c2cccc1	None
	1267	3732	43	3H-JNJ 7777120	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1267	3732	43	3H-JNJ-5207852	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	3H-JNJ 7777120	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	3035905	3732	43	3H-JNJ-5207852	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	3H-JNJ 7777120	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	CHEMBL260374	3732	43	3H-JNJ-5207852	-33	28	Human	7.1	pKi	=	7.1	Binding	NoneNone	PDSP KiDatabase	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	None
	1204	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	=	8.1	Binding	Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2Displacement of [3H]-histamine from human histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1204	1893	114	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	3H-Histamine	-3	25	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	180	397	50	None	-32	39	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	200	397	50	None	-32	39	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	2160	397	50	None	-32	39	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	CHEMBL629	397	50	None	-32	39	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	DB00321	397	50	None	-32	39	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	277	3	0	1	4.2	CN(CCC=C1c2ccccc2CCc2c1cccc2)C	None
	277	1269	55	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	2913	1269	55	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	765	1269	55	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	CHEMBL516	1269	55	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	DB00434	1269	55	None	-301	46	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	287	0	0	1	4.7	CN1CCC(=C2c3ccccc3C=Cc3c2cccc3)CC1	None
	1204	1893	114	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1247	1893	114	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	1375	1893	114	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	774	1893	114	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	CHEMBL90	1893	114	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	DB05381	1893	114	None	1	25	Guinea pig	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	111	2	2	2	-0.1	NCCc1cnc[nH]1	None
	135398737	942	89	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	None	-57	92	Human	8.1	pKi	=	8.1	Binding	NoneNone	Drug Central	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	135398737	942	89	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	3H-Histamine	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	135398737	942	89	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	38	942	89	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	722	942	89	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	CHEMBL42	942	89	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	DB00363	942	89	3H-PYRILAMINE	-57	92	Human	6.1	pKi	=	6.1	Binding	NoneNone	PDSP KiDatabase	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	None
	115237	55281	113	3H-Histamine	-891	55	Human	5.1	pKi	=	5.1	Binding	NoneNone	PDSP KiDatabase	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	CHEMBL1621	55281	113	3H-Histamine	-891	55	Human	5.1	pKi	=	5.1	Binding	NoneNone	PDSP KiDatabase	426	4	1	7	3.1	Cc1nc2n(c(=O)c1CCN1CCC(c3noc4cc(F)ccc34)CC1)CCCC2O	None
	1251	1975	34	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	3035842	1975	34	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	CHEMBL18661	1975	34	3H-Histamine	-100	5	Human	8.1	pKi	=	8.1	Binding	NoneNone	PDSP KiDatabase	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	None
	23650961	3773	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	9276	3773	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	CHEMBL3301609	3773	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	DB12522	3773	18	None	-	1	Human	7.1	pKi	=	7.1	Binding	Displacament of histamine from human H4 recepotor expressed in mammalian cells.Displacament of histamine from human H4 recepotor expressed in mammalian cells.	Guide to Pharmacology	392	6	2	5	4.5	CN1CCC(CC1)CCCNc1ncc(c(n1)C)c1nc2c([nH]1)cc(cc2C)C	None
	1265	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	1265	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	1265	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	6422124	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	6422124	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	6422124	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	CHEMBL14638	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	12606603
	CHEMBL14638	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	15294456
	CHEMBL14638	927	54	None	-2290	8	Human	5.7	pKi	=	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	270	7	1	3	3.0	O=C(c1ccc(cc1)OCCCc1[nH]cnc1)C1CC1	26084539
	1267	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	1267	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	1267	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	1267	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1267	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	1267	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	3035905	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	3035905	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	3035905	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	3035905	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	3035905	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	CHEMBL260374	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179434
	CHEMBL260374	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11179436
	CHEMBL260374	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11181941
	CHEMBL260374	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	12606603
	CHEMBL260374	3732	43	None	-33	28	Human	7.0	pKi	=	7.0	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	15294456
	10446295	2092	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	1277	2092	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	CHEMBL185951	2092	29	None	269	2	Human	7.1	pKi	=	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	278	1	1	3	1.6	CN1CCN(CC1)C(=O)c1nc2c([nH]1)cc(cc2)Cl	15454206
	1223	938	32	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	938	32	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	938	32	None	-338	11	Rat	7.2	pKi	=	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1274	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	3063228	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	CHEMBL1196470	3989	12	None	19	2	Human	7.5	pKi	=	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	3	4	3	-1.1	NC(=N)SCCN=C(N)N	17154494
	1269	121	33	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	1269	121	33	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	37463	121	33	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	37463	121	33	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	CHEMBL275443	121	33	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	15947036
	CHEMBL275443	121	33	None	213	4	Human	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.2	Cc1c(CCN)nc[nH]1	16432504
	1223	938	32	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	938	32	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	938	32	None	-363	11	Mouse	7.8	pKi	=	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1223	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	1223	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	1223	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	1223	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	1223	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	2790	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	2790	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	2790	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	2790	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	2790	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	CHEMBL14690	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11179434
	CHEMBL14690	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11181941
	CHEMBL14690	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	11561071
	CHEMBL14690	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	12606603
	CHEMBL14690	938	32	None	-60	11	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	308	6	2	3	3.2	Clc1ccc(cc1)CN=C(SCCCc1cnc[nH]1)N	15947036
	24994634	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	24994634	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	8984	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	8984	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	CHEMBL3236549	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	24495018
	CHEMBL3236549	2102	26	None	-2	7	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	2	2	5	0.7	N[C@@H]1CCN(C1)c1cc(nc(n1)N)C(C)C	26084539
	45257132	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	8983	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	CHEMBL595180	3613	3	None	42	4	Human	7.9	pKi	=	7.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	366	4	2	6	2.7	CN1CCN(CC1)c1cc(NCc2c(Cl)cccc2Cl)nc(n1)N	26084539
	1278	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1278	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	1278	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	1279	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	1279	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	1279	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	4908365	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	4908365	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	4908365	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	CHEMBL129198	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	14722321
	CHEMBL129198	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	15947036
	CHEMBL129198	2110	79	None	-3	8	Human	8.1	pKi	=	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	277	1	1	2	2.2	CN1CCN(CC1)C(=O)c1cc2c([nH]1)ccc(c2)Cl	16854056
	117072554	1984	0	None	81	4	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	450	5	2	6	2.3	CN1CCN(CC1)C1=CC(=NC(N1)N)c1ccc2c(c1)CN(CC2)C(=O)CCC1CCCC1	26084539
	8982	1984	0	None	81	4	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	450	5	2	6	2.3	CN1CCN(CC1)C1=CC(=NC(N1)N)c1ccc2c(c1)CN(CC2)C(=O)CCC1CCCC1	26084539
	24745335	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	8985	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	CHEMBL1915540	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	DB15027	292	7	None	4	5	Human	8.3	pKi	=	8.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	6	0.7	CN[C@@H]1CCN(C1)c1cc(NCC2CC2)nc(n1)N	26084539
	5048	3072	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	8924	3072	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	9948102	3072	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	CHEMBL462605	3072	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	DB11642	3072	57	None	-48977	7	Human	4.0	pKi	>	4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	295	8	0	2	4.2	Clc1ccc(cc1)CCCOCCCN1CCCCC1	26084539
	441082	1136	40	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	8981	1136	40	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	CHEMBL191703	1136	40	None	-2137	8	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	356	1	0	2	4.8	CN([C@H]1CC[C@]2(C(=CC[C@@H]3[C@@H]2CC[C@]24[C@H]3CC[C@@H]4[C@@H](N(C2)C)C)C1)C)C	26084539
	11697697	2500	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	7346	2500	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	CHEMBL476323	2500	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	DB11910	2500	19	None	-6309	6	Human	5.0	pKi	>	5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	431	6	0	5	4.6	Cc1nc2cccc(c2c(=O)n1c1ccc(cc1)OCCCN1CCCC1)C(F)(F)F	26084539
	1547484	925	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	654	925	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	9072	925	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	CHEMBL43064	925	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	DB00568	925	70	None	-5	20	Human	8.2	pKi	None	8.2	Binding	NoneNone	Drug Central	368	6	0	2	5.1	c1ccc(cc1)/C=C/CN1CCN(CC1)C(c1ccccc1)c1ccccc1	None
	10058807	1979	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	1276	1979	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	CHEMBL2261340	1979	2	None	-1	2	Human	5.2	pKi	None	5.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	206	4	3	3	-0.0	CN=C(NC#N)NCCCc1cnc[nH]1	11179436
	135398737	942	89	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	942	89	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	942	89	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	942	89	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	942	89	None	-54	92	Mouse	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	1238	2043	11	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	6603865	2043	11	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	CHEMBL11919	2043	11	None	-19	3	Human	5.5	pKi	None	5.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@@H](Cc1cnc[nH]1)N	11181941
	1248	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	1248	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	1248	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	1248	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	3077	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	3077	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	3077	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	3077	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	90045	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	90045	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	90045	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	90045	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	CHEMBL12344	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179434
	CHEMBL12344	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11179436
	CHEMBL12344	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	11181941
	CHEMBL12344	1391	27	None	-15	7	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	161	4	2	3	0.6	CN(CCCSC(=N)N)C	15947036
	135398737	942	89	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	942	89	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	942	89	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	942	89	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	942	89	None	-44	92	Rat	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	11313837	2454	7	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	1254	2454	7	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	CHEMBL175782	2454	7	None	-1995	2	Human	5.7	pKi	None	5.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	179	2	1	2	1.3	CN1CCC(CC1)Cc1[nH]cnc1	15771452
	124846	1907	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	1273	1907	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	CHEMBL67601	1907	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	382	10	3	3	4.1	CC(NCCc1cnc[nH]1)CCCCC(=O)Nc1ccc(cc1)C(F)(F)F	11179436
	12656	2001	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	1270	2001	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	CHEMBL12704	2001	0	None	-	1	Human	5.9	pKi	None	5.9	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	2	0.5	CN(CCc1[nH]cnc1)C	16432504
	1194	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	23786499	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	CHEMBL26116	46	3	None	-1318	4	Human	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	265	3	2	2	2.3	NCCc1cnc([nH]1)c1cccc(c1)Br	15947036
	1229	730	0	None	-19	6	Rat	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	730	0	None	-19	6	Rat	6.0	pKi	None	6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1229	730	0	None	-15	6	Mouse	6.1	pKi	None	6.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	730	0	None	-15	6	Mouse	6.1	pKi	None	6.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1236	2028	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2028	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2028	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2028	23	None	-851	7	Rat	6.2	pKi	None	6.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1241	1998	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	1998	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	1998	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	1998	46	None	-407	5	Rat	6.3	pKi	None	6.3	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1268	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	15947036
	1268	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	16432504
	92613	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	15947036
	92613	82	0	None	-	1	Human	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	221	4	3	5	-2.0	OCCN1CN(CO)C(=S)N(C1)CO	16432504
	1236	2028	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2028	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2028	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2028	23	None	-537	7	Mouse	6.4	pKi	None	6.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	135398737	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	135398737	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	135398737	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	135398737	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	38	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	38	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	38	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	38	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	722	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	722	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	722	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	722	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	CHEMBL42	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	CHEMBL42	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	CHEMBL42	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	CHEMBL42	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	DB00363	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179434
	DB00363	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11179436
	DB00363	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	11561071
	DB00363	942	89	None	-57	92	Human	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	326	0	1	4	3.7	CN1CCN(CC1)C1=Nc2cc(Cl)ccc2Nc2c1cccc2	15947036
	1241	1998	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	1998	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	1998	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	1998	46	None	-257	5	Mouse	6.5	pKi	None	6.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1236	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1236	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	1236	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	1236	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1237	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	1237	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	1237	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	1237	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	156615	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	156615	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	156615	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	156615	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	CHEMBL268229	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179434
	CHEMBL268229	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11179436
	CHEMBL268229	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11181941
	CHEMBL268229	2028	23	None	-204	7	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	2	2	0.3	C[C@H](Cc1cnc[nH]1)N	11561071
	1271	1997	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	44292270	1997	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	CHEMBL46296	1997	0	None	-	1	Human	6.7	pKi	None	6.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	139	3	1	3	0.4	NCCc1ncn(c1)CC	16432504
	1239	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	1239	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	1240	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	1240	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	912	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	912	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	CHEMBL268490	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11179436
	CHEMBL268490	1994	23	None	-154	5	Human	7.0	pKi	None	7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	3	2	2	0.2	CNCCc1cnc[nH]1	11181941
	1229	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179434
	1229	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179436
	1229	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11181941
	1229	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	1229	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	15947036
	5311293	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179434
	5311293	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11179436
	5311293	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11181941
	5311293	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	11561071
	5311293	730	0	None	-1	6	Human	7.1	pKi	None	7.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	262	5	3	2	2.0	CNC(=S)NCCCCc1cccc2c1cn[nH]2	15947036
	1204	1893	114	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1893	114	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1893	114	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1893	114	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1893	114	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1893	114	None	-12	25	Rat	7.2	pKi	None	7.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1893	114	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1893	114	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1893	114	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1893	114	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1893	114	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1893	114	None	-15	25	Mouse	7.4	pKi	None	7.4	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1241	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1241	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1241	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	1242	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	1242	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	1242	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	3614	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	3614	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	3614	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	CHEMBL507	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11179434
	CHEMBL507	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	11561071
	CHEMBL507	1998	46	None	-89	5	Human	7.5	pKi	None	7.5	Binding	UnclassifiedUnclassified	Guide to Pharmacology	125	2	1	3	-0.1	Cn1cc(nc1)CCN	16432504
	1267	3732	43	None	-21	28	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1267	3732	43	None	-14	28	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3732	43	None	-21	28	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	3035905	3732	43	None	-14	28	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3732	43	None	-21	28	Mouse	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	CHEMBL260374	3732	43	None	-14	28	Rat	7.6	pKi	None	7.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	292	2	2	2	2.8	S=C(N1CCC(CC1)c1cnc[nH]1)NC1CCCCC1	11561071
	1266	2018	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	3960	2018	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	5311186	2018	0	None	-23	3	Human	7.7	pKi	None	7.7	Binding	UnclassifiedUnclassified	Guide to Pharmacology	416	9	3	4	2.8	N[C@H](SCCCc1cnc[nH]1)NCCc1ccc(cc1)I	11179436
	1226	1980	16	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	1226	1980	16	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	41376	1980	16	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	41376	1980	16	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	CHEMBL12608	1980	16	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	11179434
	CHEMBL12608	1980	16	None	2	7	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	321	9	4	4	1.2	NC(=NCCCc1cnc[nH]1)NCCSCc1nc[nH]c1C	15947036
	1251	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	1251	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	1251	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	3035842	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	3035842	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	3035842	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	CHEMBL18661	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179434
	CHEMBL18661	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	11179436
	CHEMBL18661	1975	34	None	-100	5	Human	7.8	pKi	None	7.8	Binding	UnclassifiedUnclassified	Guide to Pharmacology	165	2	2	2	1.0	N1CCC(CC1)Cc1[nH]cnc1	14640553
	1204	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1204	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1204	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1204	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1204	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1204	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1204	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1247	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1247	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1247	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1247	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1247	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1247	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1247	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	1375	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	1375	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	1375	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	1375	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	1375	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	1375	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	1375	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	774	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	774	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	774	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	774	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	774	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	774	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	774	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	CHEMBL90	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179434
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11179436
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11181941
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	11561071
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15454206
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	15947036
	DB05381	1893	114	None	-3	25	Human	8.1	pKi	None	8.1	Binding	UnclassifiedUnclassified	Guide to Pharmacology	111	2	2	2	-0.1	NCCc1cnc[nH]1	16432504
	11957573	1971	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	11957573	1971	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1971	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1971	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1971	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1971	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1971	29	None	-27	6	Mouse	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1971	29	None	-27	6	Rat	8.2	pKi	None	8.2	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	11957573	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	11957573	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	11957573	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	11957573	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	1250	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	1250	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	1250	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	1250	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	3692	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	3692	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	3692	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	3692	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071
	CHEMBL19439	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179434
	CHEMBL19439	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11179436
	CHEMBL19439	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11181941
	CHEMBL19439	1971	29	None	-40	6	Human	8.6	pKi	None	8.6	Binding	UnclassifiedUnclassified	Guide to Pharmacology	170	3	3	3	0.6	NC(=N)SCCc1cnc[nH]1	11561071

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